Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS00290 and LRK53_RS00295 are separated by 50 nucleotides LRK53_RS00295 and LRK53_RS00300 are separated by 42 nucleotides
LRK53_RS00290: LRK53_RS00290 - Xaa-Pro peptidase family protein, at 67,494 to 68,855
_RS00290
LRK53_RS00295: LRK53_RS00295 - hypothetical protein, at 68,906 to 69,151
_RS00295
LRK53_RS00300: LRK53_RS00300 - undecaprenyl-diphosphate phosphatase, at 69,194 to 69,991
_RS00300
Position (kb) 68
69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 67.917 kb on + strand, within LRK53_RS00290 at 67.917 kb on + strand, within LRK53_RS00290 at 67.917 kb on + strand, within LRK53_RS00290 at 67.917 kb on + strand, within LRK53_RS00290 at 67.917 kb on + strand, within LRK53_RS00290 at 67.917 kb on + strand, within LRK53_RS00290 at 67.917 kb on + strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 67.918 kb on - strand, within LRK53_RS00290 at 68.078 kb on - strand, within LRK53_RS00290 at 68.078 kb on - strand, within LRK53_RS00290 at 68.078 kb on - strand, within LRK53_RS00290 at 68.078 kb on - strand, within LRK53_RS00290 at 68.226 kb on + strand, within LRK53_RS00290 at 68.226 kb on + strand, within LRK53_RS00290 at 68.226 kb on + strand, within LRK53_RS00290 at 68.227 kb on - strand, within LRK53_RS00290 at 68.298 kb on + strand, within LRK53_RS00290 at 68.299 kb on - strand, within LRK53_RS00290 at 68.346 kb on + strand, within LRK53_RS00290 at 68.347 kb on - strand, within LRK53_RS00290 at 68.437 kb on - strand, within LRK53_RS00290 at 68.437 kb on - strand, within LRK53_RS00290 at 68.437 kb on - strand, within LRK53_RS00290 at 68.456 kb on + strand, within LRK53_RS00290 at 68.456 kb on + strand, within LRK53_RS00290 at 68.457 kb on - strand, within LRK53_RS00290 at 68.457 kb on - strand, within LRK53_RS00290 at 68.497 kb on + strand, within LRK53_RS00290 at 68.497 kb on + strand, within LRK53_RS00290 at 68.497 kb on + strand, within LRK53_RS00290 at 68.497 kb on + strand, within LRK53_RS00290 at 68.498 kb on - strand, within LRK53_RS00290 at 68.498 kb on - strand, within LRK53_RS00290 at 68.620 kb on - strand, within LRK53_RS00290 at 68.625 kb on + strand, within LRK53_RS00290 at 68.625 kb on + strand, within LRK53_RS00290 at 68.625 kb on + strand, within LRK53_RS00290 at 68.625 kb on + strand, within LRK53_RS00290 at 68.625 kb on + strand, within LRK53_RS00290 at 68.626 kb on - strand, within LRK53_RS00290 at 68.626 kb on - strand, within LRK53_RS00290 at 68.626 kb on - strand, within LRK53_RS00290 at 68.676 kb on + strand, within LRK53_RS00290 at 68.676 kb on + strand, within LRK53_RS00290 at 68.676 kb on + strand, within LRK53_RS00290 at 68.676 kb on + strand, within LRK53_RS00290 at 68.677 kb on - strand, within LRK53_RS00290 at 68.677 kb on - strand, within LRK53_RS00290 at 68.677 kb on - strand, within LRK53_RS00290 at 68.677 kb on - strand, within LRK53_RS00290 at 68.679 kb on + strand, within LRK53_RS00290 at 68.679 kb on + strand, within LRK53_RS00290 at 68.679 kb on + strand, within LRK53_RS00290 at 68.679 kb on + strand, within LRK53_RS00290 at 68.679 kb on + strand, within LRK53_RS00290 at 68.679 kb on + strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.680 kb on - strand, within LRK53_RS00290 at 68.686 kb on + strand, within LRK53_RS00290 at 68.742 kb on - strand at 68.742 kb on - strand at 68.742 kb on - strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.745 kb on + strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.746 kb on - strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.787 kb on + strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.788 kb on - strand at 68.853 kb on + strand at 68.898 kb on + strand at 68.898 kb on + strand at 68.899 kb on - strand at 68.899 kb on - strand at 68.993 kb on + strand, within LRK53_RS00295 at 68.993 kb on + strand, within LRK53_RS00295 at 68.993 kb on + strand, within LRK53_RS00295 at 68.994 kb on - strand, within LRK53_RS00295 at 68.994 kb on - strand, within LRK53_RS00295 at 68.994 kb on - strand, within LRK53_RS00295 at 68.994 kb on - strand, within LRK53_RS00295 at 69.403 kb on - strand, within LRK53_RS00300 at 69.418 kb on - strand, within LRK53_RS00300 at 69.817 kb on - strand, within LRK53_RS00300 at 70.055 kb on + strand at 70.055 kb on + strand at 70.055 kb on + strand at 70.056 kb on - strand at 70.056 kb on - strand at 70.056 kb on - strand at 70.056 kb on - strand at 70.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 5 mM remove 67,917 + LRK53_RS00290 0.31 +1.1 67,917 + LRK53_RS00290 0.31 +0.1 67,917 + LRK53_RS00290 0.31 -0.5 67,917 + LRK53_RS00290 0.31 -0.4 67,917 + LRK53_RS00290 0.31 +2.1 67,917 + LRK53_RS00290 0.31 +0.1 67,917 + LRK53_RS00290 0.31 -0.8 67,918 - LRK53_RS00290 0.31 +0.1 67,918 - LRK53_RS00290 0.31 +1.1 67,918 - LRK53_RS00290 0.31 -0.5 67,918 - LRK53_RS00290 0.31 -2.3 67,918 - LRK53_RS00290 0.31 -1.3 67,918 - LRK53_RS00290 0.31 +0.1 67,918 - LRK53_RS00290 0.31 +0.1 67,918 - LRK53_RS00290 0.31 -0.5 67,918 - LRK53_RS00290 0.31 -1.0 68,078 - LRK53_RS00290 0.43 +0.1 68,078 - LRK53_RS00290 0.43 -0.2 68,078 - LRK53_RS00290 0.43 -1.3 68,078 - LRK53_RS00290 0.43 +2.7 68,226 + LRK53_RS00290 0.54 +0.4 68,226 + LRK53_RS00290 0.54 -3.3 68,226 + LRK53_RS00290 0.54 +0.5 68,227 - LRK53_RS00290 0.54 -1.5 68,298 + LRK53_RS00290 0.59 -0.9 68,299 - LRK53_RS00290 0.59 -2.8 68,346 + LRK53_RS00290 0.63 -1.2 68,347 - LRK53_RS00290 0.63 -0.8 68,437 - LRK53_RS00290 0.69 -1.1 68,437 - LRK53_RS00290 0.69 +1.1 68,437 - LRK53_RS00290 0.69 +1.1 68,456 + LRK53_RS00290 0.71 +0.1 68,456 + LRK53_RS00290 0.71 -1.0 68,457 - LRK53_RS00290 0.71 -0.5 68,457 - LRK53_RS00290 0.71 -1.5 68,497 + LRK53_RS00290 0.74 -1.0 68,497 + LRK53_RS00290 0.74 -0.5 68,497 + LRK53_RS00290 0.74 -1.7 68,497 + LRK53_RS00290 0.74 -2.0 68,498 - LRK53_RS00290 0.74 +0.1 68,498 - LRK53_RS00290 0.74 +0.4 68,620 - LRK53_RS00290 0.83 -0.2 68,625 + LRK53_RS00290 0.83 +0.7 68,625 + LRK53_RS00290 0.83 -0.6 68,625 + LRK53_RS00290 0.83 -0.7 68,625 + LRK53_RS00290 0.83 -0.2 68,625 + LRK53_RS00290 0.83 +1.1 68,626 - LRK53_RS00290 0.83 -0.5 68,626 - LRK53_RS00290 0.83 +0.5 68,626 - LRK53_RS00290 0.83 +0.5 68,676 + LRK53_RS00290 0.87 -1.9 68,676 + LRK53_RS00290 0.87 -0.6 68,676 + LRK53_RS00290 0.87 +1.1 68,676 + LRK53_RS00290 0.87 -2.0 68,677 - LRK53_RS00290 0.87 -0.5 68,677 - LRK53_RS00290 0.87 +0.1 68,677 - LRK53_RS00290 0.87 -0.5 68,677 - LRK53_RS00290 0.87 +0.7 68,679 + LRK53_RS00290 0.87 -0.1 68,679 + LRK53_RS00290 0.87 +1.7 68,679 + LRK53_RS00290 0.87 +0.3 68,679 + LRK53_RS00290 0.87 -1.7 68,679 + LRK53_RS00290 0.87 -2.2 68,679 + LRK53_RS00290 0.87 -0.8 68,680 - LRK53_RS00290 0.87 -0.2 68,680 - LRK53_RS00290 0.87 -0.7 68,680 - LRK53_RS00290 0.87 +1.1 68,680 - LRK53_RS00290 0.87 -2.3 68,680 - LRK53_RS00290 0.87 +0.1 68,680 - LRK53_RS00290 0.87 -0.9 68,680 - LRK53_RS00290 0.87 -0.9 68,680 - LRK53_RS00290 0.87 +2.1 68,680 - LRK53_RS00290 0.87 -0.9 68,680 - LRK53_RS00290 0.87 +0.1 68,680 - LRK53_RS00290 0.87 +0.3 68,686 + LRK53_RS00290 0.88 +0.1 68,742 - +0.2 68,742 - +0.5 68,742 - -1.5 68,745 + +0.5 68,745 + -0.6 68,745 + -0.9 68,745 + -1.5 68,745 + -0.9 68,745 + -0.2 68,745 + -0.2 68,745 + -1.5 68,745 + -1.0 68,745 + -1.9 68,745 + +1.1 68,745 + +0.5 68,745 + +0.1 68,745 + -0.5 68,745 + -0.5 68,745 + -1.5 68,745 + -2.7 68,745 + +0.9 68,745 + -0.5 68,745 + +2.1 68,745 + -0.0 68,745 + +0.1 68,745 + +1.1 68,745 + +0.1 68,745 + -1.0 68,745 + +0.5 68,746 - +1.1 68,746 - -0.8 68,746 - -0.9 68,746 - +0.4 68,746 - -0.3 68,746 - +0.3 68,746 - -0.5 68,746 - -0.9 68,746 - +1.9 68,746 - -0.5 68,746 - +1.1 68,746 - -0.3 68,746 - +2.1 68,746 - -1.2 68,746 - -0.2 68,746 - +0.5 68,787 + -1.0 68,787 + +0.1 68,787 + +0.1 68,787 + -1.5 68,787 + -0.6 68,787 + +0.1 68,787 + +0.1 68,787 + +0.0 68,787 + -0.2 68,787 + -0.5 68,787 + -1.2 68,787 + +1.1 68,787 + -2.7 68,787 + +0.4 68,787 + +0.1 68,787 + +0.1 68,787 + -2.2 68,787 + +0.4 68,787 + -1.5 68,787 + -1.2 68,787 + -0.9 68,787 + +1.1 68,787 + +0.1 68,787 + -0.2 68,787 + -0.9 68,787 + -0.5 68,787 + +1.3 68,787 + -1.1 68,787 + -1.4 68,787 + -1.2 68,787 + +0.1 68,787 + -0.5 68,787 + -1.5 68,787 + +0.1 68,787 + +0.1 68,787 + -0.5 68,787 + +2.1 68,787 + +1.1 68,787 + -1.0 68,788 - +0.4 68,788 - -1.0 68,788 - -0.9 68,788 - -0.6 68,788 - -1.2 68,788 - +0.5 68,788 - +0.9 68,788 - -1.2 68,788 - +0.1 68,788 - -1.5 68,788 - -1.0 68,788 - -0.6 68,788 - -1.0 68,788 - -0.5 68,788 - -0.2 68,788 - +1.1 68,788 - -0.5 68,788 - -0.9 68,788 - -1.2 68,788 - +1.1 68,788 - +1.1 68,788 - -0.5 68,788 - -0.2 68,788 - +0.4 68,788 - -1.4 68,788 - +0.1 68,788 - -0.1 68,788 - -1.9 68,788 - -0.5 68,788 - -0.9 68,788 - -1.4 68,788 - -0.7 68,788 - -0.9 68,788 - +0.1 68,788 - -0.5 68,788 - +1.1 68,788 - -0.5 68,788 - +0.4 68,788 - +1.1 68,788 - +0.1 68,788 - -0.9 68,853 + -1.7 68,898 + -1.2 68,898 + -0.5 68,899 - +0.1 68,899 - +1.1 68,993 + LRK53_RS00295 0.35 -2.0 68,993 + LRK53_RS00295 0.35 -0.9 68,993 + LRK53_RS00295 0.35 -1.3 68,994 - LRK53_RS00295 0.36 -2.9 68,994 - LRK53_RS00295 0.36 -0.4 68,994 - LRK53_RS00295 0.36 -1.9 68,994 - LRK53_RS00295 0.36 -0.5 69,403 - LRK53_RS00300 0.26 -0.9 69,418 - LRK53_RS00300 0.28 +1.1 69,817 - LRK53_RS00300 0.78 +1.1 70,055 + -1.2 70,055 + -0.7 70,055 + -0.9 70,056 - +0.4 70,056 - +0.7 70,056 - +2.1 70,056 - -0.5 70,056 - -1.0
Or see this region's nucleotide sequence