Experiment: G2M5_D4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HUW76_04615 and HUW76_04620 are separated by 8 nucleotides HUW76_04620 and HUW76_04625 are separated by 8 nucleotides HUW76_04625 and HUW76_04630 overlap by 11 nucleotides HUW76_04630 and HUW76_04635 are separated by 22 nucleotides
HUW76_04615: HUW76_04615 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA, at 930,663 to 932,117
_04615
HUW76_04620: HUW76_04620 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC, at 932,126 to 932,416
_04620
HUW76_04625: HUW76_04625 - rRNA pseudouridine synthase, at 932,425 to 933,129
_04625
HUW76_04630: HUW76_04630 - SMC-Scp complex subunit ScpB, at 933,119 to 933,664
_04630
HUW76_04635: HUW76_04635 - rod shape-determining protein, at 933,687 to 934,733
_04635
Position (kb) 932
933
934 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 932.129 kb on - strand at 932.484 kb on - strand at 932.569 kb on - strand, within HUW76_04625 at 932.569 kb on - strand, within HUW76_04625 at 932.901 kb on - strand, within HUW76_04625 at 933.088 kb on - strand at 933.093 kb on - strand at 933.093 kb on - strand at 933.143 kb on - strand at 933.143 kb on - strand at 933.143 kb on - strand at 933.143 kb on - strand at 933.193 kb on - strand, within HUW76_04630 at 933.252 kb on - strand, within HUW76_04630 at 933.281 kb on - strand, within HUW76_04630 at 933.378 kb on - strand, within HUW76_04630
Per-strain Table
Position Strand Gene LocusTag Fraction G2M5_D4 remove 932,129 - -1.9 932,484 - -2.4 932,569 - HUW76_04625 0.20 -2.4 932,569 - HUW76_04625 0.20 -1.3 932,901 - HUW76_04625 0.68 -2.6 933,088 - -2.8 933,093 - -2.7 933,093 - -1.6 933,143 - -2.5 933,143 - +1.2 933,143 - -3.1 933,143 - -2.7 933,193 - HUW76_04630 0.14 -2.3 933,252 - HUW76_04630 0.24 -2.4 933,281 - HUW76_04630 0.30 -2.4 933,378 - HUW76_04630 0.47 -0.6
Or see this region's nucleotide sequence