Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18450 and LRK53_RS18455 are separated by 30 nucleotides LRK53_RS18455 and LRK53_RS18460 are separated by 62 nucleotides LRK53_RS18460 and LRK53_RS18465 are separated by 136 nucleotides
LRK53_RS18450: LRK53_RS18450 - hypothetical protein, at 64,679 to 64,990
_RS18450
LRK53_RS18455: LRK53_RS18455 - DUF3761 domain-containing protein, at 65,021 to 65,623
_RS18455
LRK53_RS18460: LRK53_RS18460 - hypothetical protein, at 65,686 to 66,612
_RS18460
LRK53_RS18465: LRK53_RS18465 - hypothetical protein, at 66,749 to 67,123
_RS18465
Position (kb) 65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 64.750 kb on + strand, within LRK53_RS18450 at 64.751 kb on - strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.868 kb on + strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.869 kb on - strand, within LRK53_RS18450 at 64.895 kb on + strand, within LRK53_RS18450 at 64.895 kb on + strand, within LRK53_RS18450 at 64.896 kb on - strand, within LRK53_RS18450 at 64.896 kb on - strand, within LRK53_RS18450 at 65.020 kb on + strand at 65.020 kb on + strand at 65.020 kb on + strand at 65.020 kb on + strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.021 kb on - strand at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.180 kb on + strand, within LRK53_RS18455 at 65.181 kb on - strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.393 kb on + strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.394 kb on - strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.473 kb on + strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.474 kb on - strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.525 kb on + strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.526 kb on - strand, within LRK53_RS18455 at 65.644 kb on + strand at 65.644 kb on + strand at 65.644 kb on + strand at 65.644 kb on + strand at 65.647 kb on + strand at 65.712 kb on - strand at 65.712 kb on - strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.764 kb on + strand at 65.765 kb on - strand at 65.765 kb on - strand at 65.765 kb on - strand at 65.765 kb on - strand at 65.765 kb on - strand at 66.184 kb on + strand, within LRK53_RS18460 at 66.184 kb on + strand, within LRK53_RS18460 at 66.184 kb on + strand, within LRK53_RS18460 at 66.185 kb on - strand, within LRK53_RS18460 at 66.435 kb on - strand, within LRK53_RS18460 at 66.572 kb on + strand at 66.716 kb on + strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.717 kb on - strand at 66.761 kb on + strand at 66.794 kb on + strand, within LRK53_RS18465 at 66.794 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.927 kb on + strand, within LRK53_RS18465 at 66.928 kb on - strand, within LRK53_RS18465 at 66.928 kb on - strand, within LRK53_RS18465 at 66.949 kb on + strand, within LRK53_RS18465 at 66.949 kb on + strand, within LRK53_RS18465 at 66.949 kb on + strand, within LRK53_RS18465 at 66.950 kb on - strand, within LRK53_RS18465 at 66.950 kb on - strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.085 kb on + strand, within LRK53_RS18465 at 67.086 kb on - strand at 67.086 kb on - strand at 67.086 kb on - strand at 67.086 kb on - strand at 67.086 kb on - strand at 67.086 kb on - strand at 67.086 kb on - strand at 67.086 kb on - strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.301 kb on + strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.302 kb on - strand at 67.398 kb on + strand at 67.399 kb on - strand at 67.399 kb on - strand at 67.399 kb on - strand at 67.404 kb on + strand at 67.404 kb on + strand at 67.404 kb on + strand at 67.404 kb on + strand at 67.404 kb on + strand at 67.405 kb on - strand at 67.405 kb on - strand at 67.405 kb on - strand at 67.405 kb on - strand at 67.405 kb on - strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.572 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 64,750 + LRK53_RS18450 0.23 -1.6 64,751 - LRK53_RS18450 0.23 -0.7 64,868 + LRK53_RS18450 0.61 -0.1 64,868 + LRK53_RS18450 0.61 -0.2 64,868 + LRK53_RS18450 0.61 -0.6 64,868 + LRK53_RS18450 0.61 -1.8 64,869 - LRK53_RS18450 0.61 -0.6 64,869 - LRK53_RS18450 0.61 -0.6 64,869 - LRK53_RS18450 0.61 -0.3 64,869 - LRK53_RS18450 0.61 +0.4 64,869 - LRK53_RS18450 0.61 -0.3 64,869 - LRK53_RS18450 0.61 -1.1 64,895 + LRK53_RS18450 0.69 -0.4 64,895 + LRK53_RS18450 0.69 -0.6 64,896 - LRK53_RS18450 0.70 -1.8 64,896 - LRK53_RS18450 0.70 -1.0 65,020 + +2.0 65,020 + +0.6 65,020 + +0.2 65,020 + -0.8 65,021 - -0.5 65,021 - +1.3 65,021 - -0.1 65,180 + LRK53_RS18455 0.26 -2.4 65,180 + LRK53_RS18455 0.26 -1.0 65,180 + LRK53_RS18455 0.26 +0.8 65,180 + LRK53_RS18455 0.26 -0.7 65,181 - LRK53_RS18455 0.27 -0.4 65,393 + LRK53_RS18455 0.62 -0.8 65,393 + LRK53_RS18455 0.62 +1.0 65,393 + LRK53_RS18455 0.62 -0.8 65,394 - LRK53_RS18455 0.62 -1.3 65,394 - LRK53_RS18455 0.62 -0.2 65,394 - LRK53_RS18455 0.62 -0.4 65,473 + LRK53_RS18455 0.75 +0.0 65,473 + LRK53_RS18455 0.75 -0.6 65,473 + LRK53_RS18455 0.75 -1.6 65,473 + LRK53_RS18455 0.75 -1.2 65,474 - LRK53_RS18455 0.75 -1.8 65,474 - LRK53_RS18455 0.75 +0.0 65,474 - LRK53_RS18455 0.75 -1.4 65,474 - LRK53_RS18455 0.75 -0.3 65,474 - LRK53_RS18455 0.75 +0.0 65,474 - LRK53_RS18455 0.75 -0.3 65,525 + LRK53_RS18455 0.84 -1.3 65,525 + LRK53_RS18455 0.84 -0.5 65,525 + LRK53_RS18455 0.84 -1.0 65,525 + LRK53_RS18455 0.84 -0.8 65,525 + LRK53_RS18455 0.84 -0.2 65,525 + LRK53_RS18455 0.84 -1.0 65,525 + LRK53_RS18455 0.84 -1.0 65,525 + LRK53_RS18455 0.84 -0.7 65,525 + LRK53_RS18455 0.84 +0.1 65,526 - LRK53_RS18455 0.84 -1.6 65,526 - LRK53_RS18455 0.84 +1.0 65,526 - LRK53_RS18455 0.84 -1.0 65,526 - LRK53_RS18455 0.84 -0.5 65,644 + +1.5 65,644 + +1.0 65,644 + -0.9 65,644 + -2.0 65,647 + +0.2 65,712 - -0.5 65,712 - +0.3 65,764 + -0.2 65,764 + -0.6 65,764 + -2.0 65,764 + +0.0 65,765 - -0.8 65,765 - -0.3 65,765 - +0.0 65,765 - +0.8 65,765 - +1.0 66,184 + LRK53_RS18460 0.54 -0.1 66,184 + LRK53_RS18460 0.54 -1.8 66,184 + LRK53_RS18460 0.54 -1.0 66,185 - LRK53_RS18460 0.54 -1.0 66,435 - LRK53_RS18460 0.81 -0.8 66,572 + -0.8 66,716 + -0.5 66,717 - -0.6 66,717 - -0.8 66,717 - -0.6 66,717 - -1.8 66,761 + -0.4 66,794 + LRK53_RS18465 0.12 +0.7 66,794 + LRK53_RS18465 0.12 +0.6 66,927 + LRK53_RS18465 0.47 -0.2 66,927 + LRK53_RS18465 0.47 -1.8 66,927 + LRK53_RS18465 0.47 -1.0 66,927 + LRK53_RS18465 0.47 -1.0 66,927 + LRK53_RS18465 0.47 -1.7 66,927 + LRK53_RS18465 0.47 +1.0 66,927 + LRK53_RS18465 0.47 +0.1 66,927 + LRK53_RS18465 0.47 -0.5 66,927 + LRK53_RS18465 0.47 -0.4 66,928 - LRK53_RS18465 0.48 +0.4 66,928 - LRK53_RS18465 0.48 -1.3 66,949 + LRK53_RS18465 0.53 +0.2 66,949 + LRK53_RS18465 0.53 +0.5 66,949 + LRK53_RS18465 0.53 -0.4 66,950 - LRK53_RS18465 0.54 +0.0 66,950 - LRK53_RS18465 0.54 -0.3 67,085 + LRK53_RS18465 0.90 -1.3 67,085 + LRK53_RS18465 0.90 -0.8 67,085 + LRK53_RS18465 0.90 -2.0 67,085 + LRK53_RS18465 0.90 -0.4 67,085 + LRK53_RS18465 0.90 -1.3 67,085 + LRK53_RS18465 0.90 -1.0 67,085 + LRK53_RS18465 0.90 -0.6 67,085 + LRK53_RS18465 0.90 -1.6 67,085 + LRK53_RS18465 0.90 +1.4 67,085 + LRK53_RS18465 0.90 -0.1 67,086 - -0.4 67,086 - -0.4 67,086 - -1.6 67,086 - +0.0 67,086 - -0.2 67,086 - -2.1 67,086 - -0.4 67,086 - +1.0 67,301 + -1.6 67,301 + +0.0 67,301 + -1.0 67,301 + +0.4 67,301 + -1.0 67,301 + -0.5 67,301 + -0.8 67,301 + +0.1 67,302 - -1.1 67,302 - +2.0 67,302 - +0.1 67,302 - -0.5 67,302 - +0.4 67,302 - -0.3 67,302 - -1.3 67,302 - -0.6 67,302 - -1.6 67,302 - +1.0 67,398 + -0.1 67,399 - -1.0 67,399 - +0.5 67,399 - +0.4 67,404 + -1.0 67,404 + +1.0 67,404 + -1.0 67,404 + -1.3 67,404 + -1.0 67,405 - -1.8 67,405 - +0.2 67,405 - -0.8 67,405 - -0.1 67,405 - -1.2 67,530 + +0.0 67,530 + +0.0 67,530 + -1.0 67,530 + -2.3 67,530 + +0.4 67,530 + +1.0 67,530 + +0.0 67,530 + +0.7 67,530 + +0.0 67,531 - -0.7 67,531 - -1.9 67,531 - -1.1 67,531 - -1.3 67,531 - -1.8 67,531 - -2.0 67,531 - -1.8 67,572 + +1.0
Or see this region's nucleotide sequence