Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18145 and LRK53_RS18150 are separated by 14 nucleotides LRK53_RS18150 and LRK53_RS18155 overlap by 4 nucleotides LRK53_RS18155 and LRK53_RS18160 are separated by 156 nucleotides LRK53_RS18160 and LRK53_RS18165 are separated by 15 nucleotides
LRK53_RS18145: LRK53_RS18145 - DHHA1 domain-containing protein, at 2,971 to 4,749
_RS18145
LRK53_RS18150: LRK53_RS18150 - hypothetical protein, at 4,764 to 5,423
_RS18150
LRK53_RS18155: LRK53_RS18155 - hypothetical protein, at 5,420 to 6,601
_RS18155
LRK53_RS18160: LRK53_RS18160 - thermonuclease family protein, at 6,758 to 7,480
_RS18160
LRK53_RS18165: LRK53_RS18165 - hypothetical protein, at 7,496 to 8,482
_RS18165
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4.673 kb on - strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.758 kb on + strand at 4.759 kb on - strand at 4.759 kb on - strand at 4.759 kb on - strand at 4.759 kb on - strand at 4.759 kb on - strand at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.197 kb on + strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.198 kb on - strand, within LRK53_RS18150 at 5.421 kb on + strand at 5.421 kb on + strand at 5.421 kb on + strand at 5.421 kb on + strand at 5.422 kb on - strand at 5.422 kb on - strand at 5.422 kb on - strand at 5.425 kb on + strand at 5.425 kb on + strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.426 kb on - strand at 5.622 kb on - strand, within LRK53_RS18155 at 5.979 kb on + strand, within LRK53_RS18155 at 6.036 kb on - strand, within LRK53_RS18155 at 6.179 kb on + strand, within LRK53_RS18155 at 6.334 kb on - strand, within LRK53_RS18155 at 6.581 kb on + strand at 6.631 kb on + strand at 6.632 kb on - strand at 6.728 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.753 kb on + strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.754 kb on - strand at 6.842 kb on + strand, within LRK53_RS18160 at 6.842 kb on + strand, within LRK53_RS18160 at 6.842 kb on + strand, within LRK53_RS18160 at 7.202 kb on + strand, within LRK53_RS18160 at 7.202 kb on + strand, within LRK53_RS18160 at 7.202 kb on + strand, within LRK53_RS18160 at 7.202 kb on + strand, within LRK53_RS18160 at 7.202 kb on + strand, within LRK53_RS18160 at 7.202 kb on + strand, within LRK53_RS18160 at 7.203 kb on - strand, within LRK53_RS18160 at 7.225 kb on - strand, within LRK53_RS18160 at 7.251 kb on - strand, within LRK53_RS18160 at 7.388 kb on + strand, within LRK53_RS18160 at 7.388 kb on + strand, within LRK53_RS18160 at 7.388 kb on + strand, within LRK53_RS18160 at 7.389 kb on - strand, within LRK53_RS18160 at 7.389 kb on - strand, within LRK53_RS18160 at 7.497 kb on + strand at 7.497 kb on + strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.498 kb on - strand at 7.499 kb on + strand at 7.500 kb on - strand at 7.500 kb on - strand at 7.500 kb on - strand at 7.587 kb on + strand at 7.587 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 4,673 - +0.4 4,758 + -1.8 4,758 + -1.1 4,758 + -1.6 4,758 + +0.2 4,758 + +0.6 4,758 + +0.0 4,759 - -1.2 4,759 - -0.5 4,759 - +0.7 4,759 - -0.6 4,759 - -0.8 5,197 + LRK53_RS18150 0.66 +0.3 5,197 + LRK53_RS18150 0.66 -0.5 5,197 + LRK53_RS18150 0.66 +0.0 5,197 + LRK53_RS18150 0.66 -0.3 5,197 + LRK53_RS18150 0.66 -1.0 5,197 + LRK53_RS18150 0.66 -0.3 5,197 + LRK53_RS18150 0.66 -0.6 5,197 + LRK53_RS18150 0.66 -0.6 5,197 + LRK53_RS18150 0.66 -1.6 5,197 + LRK53_RS18150 0.66 -1.3 5,197 + LRK53_RS18150 0.66 +0.1 5,197 + LRK53_RS18150 0.66 -0.9 5,198 - LRK53_RS18150 0.66 -0.4 5,198 - LRK53_RS18150 0.66 -0.7 5,198 - LRK53_RS18150 0.66 -1.1 5,198 - LRK53_RS18150 0.66 -0.9 5,198 - LRK53_RS18150 0.66 +1.8 5,198 - LRK53_RS18150 0.66 -1.1 5,198 - LRK53_RS18150 0.66 -1.1 5,198 - LRK53_RS18150 0.66 +1.0 5,198 - LRK53_RS18150 0.66 +0.3 5,198 - LRK53_RS18150 0.66 -0.3 5,198 - LRK53_RS18150 0.66 +0.0 5,198 - LRK53_RS18150 0.66 -0.3 5,421 + -1.6 5,421 + -0.1 5,421 + +1.0 5,421 + -0.7 5,422 - +0.0 5,422 - -1.1 5,422 - +0.0 5,425 + -0.1 5,425 + -0.6 5,426 - +1.6 5,426 - +0.0 5,426 - -0.4 5,426 - -1.3 5,426 - -0.9 5,622 - LRK53_RS18155 0.17 +0.0 5,979 + LRK53_RS18155 0.47 -0.1 6,036 - LRK53_RS18155 0.52 -2.3 6,179 + LRK53_RS18155 0.64 -1.6 6,334 - LRK53_RS18155 0.77 -0.8 6,581 + +1.0 6,631 + +0.0 6,632 - +0.0 6,728 + -0.4 6,753 + -0.7 6,753 + -0.6 6,753 + -1.8 6,753 + +0.0 6,753 + -1.8 6,753 + -1.0 6,753 + +0.4 6,753 + -2.0 6,753 + -3.0 6,753 + -0.6 6,753 + +0.3 6,753 + +0.0 6,754 - -1.6 6,754 - +0.0 6,754 - -1.0 6,754 - +0.0 6,754 - +0.9 6,754 - -0.5 6,754 - -1.2 6,754 - -2.3 6,754 - -0.1 6,754 - -0.4 6,754 - -0.7 6,754 - +0.6 6,754 - -0.3 6,754 - -0.3 6,754 - -1.6 6,754 - -0.3 6,754 - -0.9 6,842 + LRK53_RS18160 0.12 -0.7 6,842 + LRK53_RS18160 0.12 -0.5 6,842 + LRK53_RS18160 0.12 +0.0 7,202 + LRK53_RS18160 0.61 -1.0 7,202 + LRK53_RS18160 0.61 +1.6 7,202 + LRK53_RS18160 0.61 -0.8 7,202 + LRK53_RS18160 0.61 -0.7 7,202 + LRK53_RS18160 0.61 +0.2 7,202 + LRK53_RS18160 0.61 -2.0 7,203 - LRK53_RS18160 0.62 -1.3 7,225 - LRK53_RS18160 0.65 -0.6 7,251 - LRK53_RS18160 0.68 +0.0 7,388 + LRK53_RS18160 0.87 +1.0 7,388 + LRK53_RS18160 0.87 +0.0 7,388 + LRK53_RS18160 0.87 -0.7 7,389 - LRK53_RS18160 0.87 +0.0 7,389 - LRK53_RS18160 0.87 -0.6 7,497 + -0.8 7,497 + -1.0 7,498 - -1.3 7,498 - -1.4 7,498 - -0.6 7,498 - -1.0 7,498 - +2.0 7,498 - -1.6 7,498 - +0.0 7,498 - -2.9 7,499 + -0.2 7,500 - -2.3 7,500 - -1.0 7,500 - +0.0 7,587 + -0.2 7,587 + +1.0
Or see this region's nucleotide sequence