Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS18020 and LRK53_RS18025 are separated by 427 nucleotides LRK53_RS18025 and LRK53_RS18030 are separated by 87 nucleotides LRK53_RS18030 and LRK53_RS18035 are separated by 103 nucleotides
LRK53_RS18020: LRK53_RS18020 - integron integrase, at 3,966,641 to 3,967,688
_RS18020
LRK53_RS18025: LRK53_RS18025 - hypothetical protein, at 3,968,116 to 3,968,304
_RS18025
LRK53_RS18030: LRK53_RS18030 - hypothetical protein, at 3,968,392 to 3,968,832
_RS18030
LRK53_RS18035: LRK53_RS18035 - hypothetical protein, at 3,968,936 to 3,969,421
_RS18035
Position (kb)
3968
3969 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3967.261 kb on + strand, within LRK53_RS18020 at 3967.261 kb on + strand, within LRK53_RS18020 at 3967.261 kb on + strand, within LRK53_RS18020 at 3967.261 kb on + strand, within LRK53_RS18020 at 3967.261 kb on + strand, within LRK53_RS18020 at 3967.262 kb on - strand, within LRK53_RS18020 at 3967.262 kb on - strand, within LRK53_RS18020 at 3967.262 kb on - strand, within LRK53_RS18020 at 3967.262 kb on - strand, within LRK53_RS18020 at 3967.414 kb on + strand, within LRK53_RS18020 at 3967.415 kb on - strand, within LRK53_RS18020 at 3967.415 kb on - strand, within LRK53_RS18020 at 3967.415 kb on - strand, within LRK53_RS18020 at 3967.415 kb on - strand, within LRK53_RS18020 at 3967.484 kb on + strand, within LRK53_RS18020 at 3967.485 kb on - strand, within LRK53_RS18020 at 3967.485 kb on - strand, within LRK53_RS18020 at 3967.551 kb on - strand, within LRK53_RS18020 at 3967.551 kb on - strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.573 kb on + strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.574 kb on - strand, within LRK53_RS18020 at 3967.598 kb on - strand at 3967.598 kb on - strand at 3967.598 kb on - strand at 3967.598 kb on - strand at 3967.659 kb on + strand at 3967.660 kb on - strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.688 kb on + strand at 3967.689 kb on - strand at 3967.689 kb on - strand at 3967.689 kb on - strand at 3967.689 kb on - strand at 3967.689 kb on - strand at 3967.771 kb on + strand at 3967.771 kb on + strand at 3967.771 kb on + strand at 3967.772 kb on - strand at 3967.772 kb on - strand at 3967.811 kb on - strand at 3967.811 kb on - strand at 3967.811 kb on - strand at 3967.840 kb on + strand at 3967.841 kb on - strand at 3967.844 kb on + strand at 3967.844 kb on + strand at 3967.844 kb on + strand at 3967.845 kb on - strand at 3967.845 kb on - strand at 3967.857 kb on + strand at 3967.858 kb on - strand at 3967.872 kb on + strand at 3967.872 kb on + strand at 3967.873 kb on - strand at 3967.873 kb on - strand at 3967.873 kb on - strand at 3967.914 kb on + strand at 3967.914 kb on + strand at 3967.915 kb on - strand at 3967.915 kb on - strand at 3967.915 kb on - strand at 3967.915 kb on - strand at 3967.916 kb on + strand at 3967.916 kb on + strand at 3967.917 kb on - strand at 3967.917 kb on - strand at 3967.948 kb on + strand at 3967.999 kb on + strand at 3967.999 kb on + strand at 3967.999 kb on + strand at 3967.999 kb on + strand at 3967.999 kb on + strand at 3968.057 kb on + strand at 3968.058 kb on - strand at 3968.058 kb on - strand at 3968.058 kb on - strand at 3968.058 kb on - strand at 3968.062 kb on + strand at 3968.153 kb on - strand, within LRK53_RS18025 at 3968.228 kb on + strand, within LRK53_RS18025 at 3968.229 kb on - strand, within LRK53_RS18025 at 3968.406 kb on - strand at 3968.406 kb on - strand at 3968.406 kb on - strand at 3968.423 kb on + strand at 3968.446 kb on - strand, within LRK53_RS18030 at 3968.531 kb on + strand, within LRK53_RS18030 at 3968.531 kb on + strand, within LRK53_RS18030 at 3968.532 kb on - strand, within LRK53_RS18030 at 3968.532 kb on - strand, within LRK53_RS18030 at 3968.532 kb on - strand, within LRK53_RS18030 at 3968.532 kb on - strand, within LRK53_RS18030 at 3968.548 kb on + strand, within LRK53_RS18030 at 3968.549 kb on - strand, within LRK53_RS18030 at 3968.549 kb on - strand, within LRK53_RS18030 at 3968.669 kb on + strand, within LRK53_RS18030 at 3968.670 kb on - strand, within LRK53_RS18030 at 3968.684 kb on - strand, within LRK53_RS18030 at 3968.684 kb on - strand, within LRK53_RS18030 at 3968.684 kb on - strand, within LRK53_RS18030 at 3968.684 kb on - strand, within LRK53_RS18030 at 3968.684 kb on - strand, within LRK53_RS18030 at 3968.687 kb on + strand, within LRK53_RS18030 at 3968.688 kb on - strand, within LRK53_RS18030 at 3968.688 kb on - strand, within LRK53_RS18030 at 3968.704 kb on + strand, within LRK53_RS18030 at 3968.704 kb on + strand, within LRK53_RS18030 at 3968.704 kb on + strand, within LRK53_RS18030 at 3968.705 kb on - strand, within LRK53_RS18030 at 3968.705 kb on - strand, within LRK53_RS18030 at 3968.705 kb on - strand, within LRK53_RS18030 at 3968.741 kb on + strand, within LRK53_RS18030 at 3968.742 kb on - strand, within LRK53_RS18030 at 3968.742 kb on - strand, within LRK53_RS18030 at 3968.742 kb on - strand, within LRK53_RS18030 at 3968.813 kb on + strand at 3968.813 kb on + strand at 3968.813 kb on + strand at 3968.813 kb on + strand at 3968.814 kb on - strand at 3968.822 kb on + strand at 3968.822 kb on + strand at 3968.822 kb on + strand at 3968.823 kb on - strand at 3968.830 kb on + strand at 3968.830 kb on + strand at 3968.831 kb on - strand at 3968.856 kb on - strand at 3968.867 kb on + strand at 3968.867 kb on + strand at 3968.867 kb on + strand at 3968.867 kb on + strand at 3968.868 kb on - strand at 3968.868 kb on - strand at 3968.935 kb on + strand at 3968.935 kb on + strand at 3968.935 kb on + strand at 3968.936 kb on - strand at 3969.012 kb on + strand, within LRK53_RS18035 at 3969.013 kb on - strand, within LRK53_RS18035 at 3969.026 kb on - strand, within LRK53_RS18035 at 3969.051 kb on - strand, within LRK53_RS18035 at 3969.051 kb on - strand, within LRK53_RS18035 at 3969.057 kb on + strand, within LRK53_RS18035 at 3969.057 kb on + strand, within LRK53_RS18035 at 3969.057 kb on + strand, within LRK53_RS18035 at 3969.057 kb on + strand, within LRK53_RS18035 at 3969.057 kb on + strand, within LRK53_RS18035 at 3969.058 kb on - strand, within LRK53_RS18035 at 3969.058 kb on - strand, within LRK53_RS18035 at 3969.058 kb on - strand, within LRK53_RS18035 at 3969.058 kb on - strand, within LRK53_RS18035 at 3969.065 kb on - strand, within LRK53_RS18035 at 3969.082 kb on + strand, within LRK53_RS18035 at 3969.116 kb on + strand, within LRK53_RS18035 at 3969.133 kb on + strand, within LRK53_RS18035 at 3969.134 kb on - strand, within LRK53_RS18035 at 3969.134 kb on - strand, within LRK53_RS18035 at 3969.134 kb on - strand, within LRK53_RS18035 at 3969.134 kb on - strand, within LRK53_RS18035 at 3969.179 kb on - strand, within LRK53_RS18035 at 3969.190 kb on + strand, within LRK53_RS18035 at 3969.191 kb on - strand, within LRK53_RS18035 at 3969.213 kb on - strand, within LRK53_RS18035 at 3969.221 kb on + strand, within LRK53_RS18035 at 3969.222 kb on - strand, within LRK53_RS18035 at 3969.236 kb on + strand, within LRK53_RS18035 at 3969.236 kb on + strand, within LRK53_RS18035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,967,261 + LRK53_RS18020 0.59 -1.3 3,967,261 + LRK53_RS18020 0.59 -2.7 3,967,261 + LRK53_RS18020 0.59 -1.0 3,967,261 + LRK53_RS18020 0.59 -0.4 3,967,261 + LRK53_RS18020 0.59 -0.8 3,967,262 - LRK53_RS18020 0.59 +0.4 3,967,262 - LRK53_RS18020 0.59 -0.6 3,967,262 - LRK53_RS18020 0.59 -1.4 3,967,262 - LRK53_RS18020 0.59 +0.6 3,967,414 + LRK53_RS18020 0.74 -0.0 3,967,415 - LRK53_RS18020 0.74 -0.6 3,967,415 - LRK53_RS18020 0.74 -0.6 3,967,415 - LRK53_RS18020 0.74 -0.0 3,967,415 - LRK53_RS18020 0.74 -0.8 3,967,484 + LRK53_RS18020 0.80 +2.6 3,967,485 - LRK53_RS18020 0.81 -0.2 3,967,485 - LRK53_RS18020 0.81 -0.2 3,967,551 - LRK53_RS18020 0.87 -1.3 3,967,551 - LRK53_RS18020 0.87 -0.0 3,967,573 + LRK53_RS18020 0.89 -0.0 3,967,573 + LRK53_RS18020 0.89 +0.7 3,967,573 + LRK53_RS18020 0.89 +0.2 3,967,573 + LRK53_RS18020 0.89 -0.5 3,967,573 + LRK53_RS18020 0.89 -1.0 3,967,573 + LRK53_RS18020 0.89 -1.5 3,967,573 + LRK53_RS18020 0.89 -0.8 3,967,573 + LRK53_RS18020 0.89 -0.3 3,967,573 + LRK53_RS18020 0.89 +0.6 3,967,573 + LRK53_RS18020 0.89 +2.0 3,967,573 + LRK53_RS18020 0.89 -0.6 3,967,573 + LRK53_RS18020 0.89 -0.8 3,967,573 + LRK53_RS18020 0.89 +1.0 3,967,573 + LRK53_RS18020 0.89 -1.6 3,967,574 - LRK53_RS18020 0.89 -2.2 3,967,574 - LRK53_RS18020 0.89 -0.3 3,967,574 - LRK53_RS18020 0.89 +0.4 3,967,574 - LRK53_RS18020 0.89 +1.6 3,967,574 - LRK53_RS18020 0.89 -2.0 3,967,574 - LRK53_RS18020 0.89 -0.0 3,967,574 - LRK53_RS18020 0.89 -0.0 3,967,574 - LRK53_RS18020 0.89 -1.7 3,967,574 - LRK53_RS18020 0.89 -1.3 3,967,574 - LRK53_RS18020 0.89 -0.4 3,967,574 - LRK53_RS18020 0.89 -0.0 3,967,574 - LRK53_RS18020 0.89 -0.7 3,967,574 - LRK53_RS18020 0.89 -2.8 3,967,598 - -0.8 3,967,598 - -1.8 3,967,598 - -0.7 3,967,598 - +1.0 3,967,659 + +1.0 3,967,660 - -0.0 3,967,688 + +0.3 3,967,688 + -0.0 3,967,688 + +0.4 3,967,688 + -0.6 3,967,688 + -0.6 3,967,688 + -0.3 3,967,688 + -0.4 3,967,688 + +0.1 3,967,688 + -1.4 3,967,688 + -0.9 3,967,688 + -0.0 3,967,689 - -1.6 3,967,689 - -0.0 3,967,689 - +0.4 3,967,689 - -2.2 3,967,689 - +2.0 3,967,771 + +2.0 3,967,771 + -0.1 3,967,771 + -1.0 3,967,772 - -0.6 3,967,772 - -1.6 3,967,811 - -0.8 3,967,811 - -1.0 3,967,811 - -0.3 3,967,840 + -1.2 3,967,841 - -0.0 3,967,844 + -0.0 3,967,844 + +2.0 3,967,844 + -1.6 3,967,845 - -0.8 3,967,845 - -0.7 3,967,857 + -1.3 3,967,858 - +0.7 3,967,872 + -0.0 3,967,872 + -2.2 3,967,873 - -0.0 3,967,873 - -0.6 3,967,873 - +0.4 3,967,914 + -0.9 3,967,914 + -0.4 3,967,915 - -0.6 3,967,915 - -0.0 3,967,915 - -0.4 3,967,915 - +0.3 3,967,916 + -0.0 3,967,916 + -0.0 3,967,917 - +0.3 3,967,917 - +0.1 3,967,948 + -1.3 3,967,999 + -0.3 3,967,999 + -0.0 3,967,999 + +0.4 3,967,999 + -0.3 3,967,999 + -0.2 3,968,057 + -0.0 3,968,058 - -0.2 3,968,058 - -0.0 3,968,058 - -0.4 3,968,058 - -0.7 3,968,062 + -0.8 3,968,153 - LRK53_RS18025 0.20 -1.4 3,968,228 + LRK53_RS18025 0.59 -1.3 3,968,229 - LRK53_RS18025 0.60 -0.6 3,968,406 - -0.7 3,968,406 - -0.0 3,968,406 - -0.1 3,968,423 + +1.0 3,968,446 - LRK53_RS18030 0.12 -1.3 3,968,531 + LRK53_RS18030 0.32 -0.4 3,968,531 + LRK53_RS18030 0.32 -0.7 3,968,532 - LRK53_RS18030 0.32 -1.6 3,968,532 - LRK53_RS18030 0.32 -0.8 3,968,532 - LRK53_RS18030 0.32 -0.0 3,968,532 - LRK53_RS18030 0.32 -0.9 3,968,548 + LRK53_RS18030 0.35 +0.3 3,968,549 - LRK53_RS18030 0.36 -0.0 3,968,549 - LRK53_RS18030 0.36 +0.6 3,968,669 + LRK53_RS18030 0.63 -1.6 3,968,670 - LRK53_RS18030 0.63 -0.6 3,968,684 - LRK53_RS18030 0.66 -0.5 3,968,684 - LRK53_RS18030 0.66 -1.3 3,968,684 - LRK53_RS18030 0.66 -0.6 3,968,684 - LRK53_RS18030 0.66 +0.3 3,968,684 - LRK53_RS18030 0.66 -2.3 3,968,687 + LRK53_RS18030 0.67 -0.0 3,968,688 - LRK53_RS18030 0.67 -0.0 3,968,688 - LRK53_RS18030 0.67 -0.4 3,968,704 + LRK53_RS18030 0.71 -1.3 3,968,704 + LRK53_RS18030 0.71 -1.0 3,968,704 + LRK53_RS18030 0.71 -0.7 3,968,705 - LRK53_RS18030 0.71 -0.0 3,968,705 - LRK53_RS18030 0.71 +0.7 3,968,705 - LRK53_RS18030 0.71 -2.0 3,968,741 + LRK53_RS18030 0.79 -0.8 3,968,742 - LRK53_RS18030 0.79 +0.1 3,968,742 - LRK53_RS18030 0.79 -1.4 3,968,742 - LRK53_RS18030 0.79 -0.5 3,968,813 + -0.0 3,968,813 + -0.0 3,968,813 + -0.0 3,968,813 + -1.3 3,968,814 - -0.6 3,968,822 + +0.4 3,968,822 + -0.6 3,968,822 + -0.3 3,968,823 - +0.7 3,968,830 + -1.3 3,968,830 + -0.2 3,968,831 - -0.3 3,968,856 - -1.0 3,968,867 + +0.3 3,968,867 + -0.5 3,968,867 + +1.0 3,968,867 + +1.0 3,968,868 - -1.3 3,968,868 - +1.0 3,968,935 + +1.6 3,968,935 + +2.0 3,968,935 + -0.2 3,968,936 - -0.0 3,969,012 + LRK53_RS18035 0.16 +0.1 3,969,013 - LRK53_RS18035 0.16 +2.0 3,969,026 - LRK53_RS18035 0.19 -0.6 3,969,051 - LRK53_RS18035 0.24 -0.0 3,969,051 - LRK53_RS18035 0.24 -0.6 3,969,057 + LRK53_RS18035 0.25 -0.0 3,969,057 + LRK53_RS18035 0.25 -0.5 3,969,057 + LRK53_RS18035 0.25 -0.4 3,969,057 + LRK53_RS18035 0.25 -0.6 3,969,057 + LRK53_RS18035 0.25 +2.0 3,969,058 - LRK53_RS18035 0.25 -0.9 3,969,058 - LRK53_RS18035 0.25 -0.6 3,969,058 - LRK53_RS18035 0.25 -0.6 3,969,058 - LRK53_RS18035 0.25 -1.0 3,969,065 - LRK53_RS18035 0.27 -1.0 3,969,082 + LRK53_RS18035 0.30 -1.0 3,969,116 + LRK53_RS18035 0.37 -2.5 3,969,133 + LRK53_RS18035 0.41 +0.4 3,969,134 - LRK53_RS18035 0.41 -0.5 3,969,134 - LRK53_RS18035 0.41 -0.9 3,969,134 - LRK53_RS18035 0.41 -0.0 3,969,134 - LRK53_RS18035 0.41 -0.0 3,969,179 - LRK53_RS18035 0.50 +0.3 3,969,190 + LRK53_RS18035 0.52 -0.6 3,969,191 - LRK53_RS18035 0.52 +1.0 3,969,213 - LRK53_RS18035 0.57 -0.8 3,969,221 + LRK53_RS18035 0.59 +1.0 3,969,222 - LRK53_RS18035 0.59 -0.0 3,969,236 + LRK53_RS18035 0.62 -0.3 3,969,236 + LRK53_RS18035 0.62 -1.0
Or see this region's nucleotide sequence