Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS17875 and LRK53_RS17880 are separated by 67 nucleotides LRK53_RS17880 and LRK53_RS17885 are separated by 154 nucleotides LRK53_RS17885 and LRK53_RS17890 are separated by 109 nucleotides
LRK53_RS17875: LRK53_RS17875 - MFS transporter, at 3,927,045 to 3,928,280
_RS17875
LRK53_RS17880: LRK53_RS17880 - exodeoxyribonuclease III, at 3,928,348 to 3,929,118
_RS17880
LRK53_RS17885: LRK53_RS17885 - orotate phosphoribosyltransferase, at 3,929,273 to 3,929,920
_RS17885
LRK53_RS17890: LRK53_RS17890 - hypothetical protein, at 3,930,030 to 3,930,674
_RS17890
Position (kb)
3928
3929
3930 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3927.388 kb on + strand, within LRK53_RS17875 at 3927.388 kb on + strand, within LRK53_RS17875 at 3927.388 kb on + strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.505 kb on + strand, within LRK53_RS17875 at 3927.506 kb on - strand, within LRK53_RS17875 at 3927.506 kb on - strand, within LRK53_RS17875 at 3927.796 kb on + strand, within LRK53_RS17875 at 3927.796 kb on + strand, within LRK53_RS17875 at 3927.797 kb on - strand, within LRK53_RS17875 at 3927.880 kb on + strand, within LRK53_RS17875 at 3927.881 kb on - strand, within LRK53_RS17875 at 3927.881 kb on - strand, within LRK53_RS17875 at 3927.931 kb on + strand, within LRK53_RS17875 at 3928.153 kb on + strand, within LRK53_RS17875 at 3928.153 kb on + strand, within LRK53_RS17875 at 3928.153 kb on + strand, within LRK53_RS17875 at 3928.153 kb on + strand, within LRK53_RS17875 at 3928.154 kb on - strand, within LRK53_RS17875 at 3928.154 kb on - strand, within LRK53_RS17875 at 3928.154 kb on - strand, within LRK53_RS17875 at 3928.154 kb on - strand, within LRK53_RS17875 at 3928.154 kb on - strand, within LRK53_RS17875 at 3928.219 kb on + strand at 3928.219 kb on + strand at 3928.315 kb on - strand at 3928.358 kb on + strand at 3928.359 kb on - strand at 3928.359 kb on - strand at 3928.370 kb on + strand at 3928.370 kb on + strand at 3928.371 kb on - strand at 3928.403 kb on + strand at 3928.404 kb on - strand at 3928.433 kb on - strand, within LRK53_RS17880 at 3928.514 kb on + strand, within LRK53_RS17880 at 3928.514 kb on + strand, within LRK53_RS17880 at 3928.688 kb on + strand, within LRK53_RS17880 at 3928.688 kb on + strand, within LRK53_RS17880 at 3928.688 kb on + strand, within LRK53_RS17880 at 3928.689 kb on - strand, within LRK53_RS17880 at 3928.689 kb on - strand, within LRK53_RS17880 at 3928.689 kb on - strand, within LRK53_RS17880 at 3928.689 kb on - strand, within LRK53_RS17880 at 3928.793 kb on + strand, within LRK53_RS17880 at 3928.793 kb on + strand, within LRK53_RS17880 at 3928.793 kb on + strand, within LRK53_RS17880 at 3928.793 kb on + strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.794 kb on - strand, within LRK53_RS17880 at 3928.836 kb on - strand, within LRK53_RS17880 at 3928.836 kb on - strand, within LRK53_RS17880 at 3928.836 kb on - strand, within LRK53_RS17880 at 3928.904 kb on + strand, within LRK53_RS17880 at 3928.904 kb on + strand, within LRK53_RS17880 at 3928.904 kb on + strand, within LRK53_RS17880 at 3928.905 kb on - strand, within LRK53_RS17880 at 3928.905 kb on - strand, within LRK53_RS17880 at 3928.905 kb on - strand, within LRK53_RS17880 at 3928.923 kb on - strand, within LRK53_RS17880 at 3928.923 kb on - strand, within LRK53_RS17880 at 3928.949 kb on + strand, within LRK53_RS17880 at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.146 kb on + strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.147 kb on - strand at 3929.205 kb on + strand at 3929.205 kb on + strand at 3929.205 kb on + strand at 3929.205 kb on + strand at 3929.205 kb on + strand at 3929.205 kb on + strand at 3929.205 kb on + strand at 3929.206 kb on - strand at 3929.206 kb on - strand at 3929.206 kb on - strand at 3929.206 kb on - strand at 3929.206 kb on - strand at 3929.206 kb on - strand at 3929.206 kb on - strand at 3929.224 kb on + strand at 3929.283 kb on - strand at 3929.283 kb on - strand at 3929.369 kb on + strand, within LRK53_RS17885 at 3929.369 kb on + strand, within LRK53_RS17885 at 3929.369 kb on + strand, within LRK53_RS17885 at 3929.370 kb on - strand, within LRK53_RS17885 at 3929.486 kb on + strand, within LRK53_RS17885 at 3929.486 kb on + strand, within LRK53_RS17885 at 3929.486 kb on + strand, within LRK53_RS17885 at 3929.561 kb on + strand, within LRK53_RS17885 at 3929.891 kb on + strand at 3929.981 kb on + strand at 3929.981 kb on + strand at 3929.981 kb on + strand at 3929.981 kb on + strand at 3929.981 kb on + strand at 3929.981 kb on + strand at 3929.982 kb on - strand at 3929.982 kb on - strand at 3929.982 kb on - strand at 3929.982 kb on - strand at 3929.982 kb on - strand at 3930.003 kb on + strand at 3930.003 kb on + strand at 3930.003 kb on + strand at 3930.004 kb on - strand at 3930.004 kb on - strand at 3930.004 kb on - strand at 3930.004 kb on - strand at 3930.004 kb on - strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.005 kb on + strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.006 kb on - strand at 3930.025 kb on + strand at 3930.025 kb on + strand at 3930.025 kb on + strand at 3930.114 kb on + strand, within LRK53_RS17890 at 3930.114 kb on + strand, within LRK53_RS17890 at 3930.114 kb on + strand, within LRK53_RS17890 at 3930.114 kb on + strand, within LRK53_RS17890 at 3930.114 kb on + strand, within LRK53_RS17890 at 3930.114 kb on + strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890 at 3930.115 kb on - strand, within LRK53_RS17890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,927,388 + LRK53_RS17875 0.28 -0.0 3,927,388 + LRK53_RS17875 0.28 +1.0 3,927,388 + LRK53_RS17875 0.28 -1.3 3,927,389 - LRK53_RS17875 0.28 +1.0 3,927,389 - LRK53_RS17875 0.28 +2.0 3,927,389 - LRK53_RS17875 0.28 -0.0 3,927,389 - LRK53_RS17875 0.28 +0.3 3,927,505 + LRK53_RS17875 0.37 -0.6 3,927,506 - LRK53_RS17875 0.37 -0.8 3,927,506 - LRK53_RS17875 0.37 -0.0 3,927,796 + LRK53_RS17875 0.61 -0.6 3,927,796 + LRK53_RS17875 0.61 -0.0 3,927,797 - LRK53_RS17875 0.61 +2.0 3,927,880 + LRK53_RS17875 0.68 +3.0 3,927,881 - LRK53_RS17875 0.68 -0.2 3,927,881 - LRK53_RS17875 0.68 -0.0 3,927,931 + LRK53_RS17875 0.72 -0.0 3,928,153 + LRK53_RS17875 0.90 +1.0 3,928,153 + LRK53_RS17875 0.90 -0.0 3,928,153 + LRK53_RS17875 0.90 -1.4 3,928,153 + LRK53_RS17875 0.90 -0.6 3,928,154 - LRK53_RS17875 0.90 -0.0 3,928,154 - LRK53_RS17875 0.90 +1.6 3,928,154 - LRK53_RS17875 0.90 +1.0 3,928,154 - LRK53_RS17875 0.90 +0.4 3,928,154 - LRK53_RS17875 0.90 -1.1 3,928,219 + +1.0 3,928,219 + +1.0 3,928,315 - +1.0 3,928,358 + +0.3 3,928,359 - +2.0 3,928,359 - -0.4 3,928,370 + -0.6 3,928,370 + -0.3 3,928,371 - -1.2 3,928,403 + -0.6 3,928,404 - +2.0 3,928,433 - LRK53_RS17880 0.11 -1.6 3,928,514 + LRK53_RS17880 0.22 +1.0 3,928,514 + LRK53_RS17880 0.22 -1.0 3,928,688 + LRK53_RS17880 0.44 +1.0 3,928,688 + LRK53_RS17880 0.44 -0.0 3,928,688 + LRK53_RS17880 0.44 -0.6 3,928,689 - LRK53_RS17880 0.44 -0.0 3,928,689 - LRK53_RS17880 0.44 -1.2 3,928,689 - LRK53_RS17880 0.44 +0.4 3,928,689 - LRK53_RS17880 0.44 -1.7 3,928,793 + LRK53_RS17880 0.58 +0.3 3,928,793 + LRK53_RS17880 0.58 -1.3 3,928,793 + LRK53_RS17880 0.58 -0.0 3,928,793 + LRK53_RS17880 0.58 -0.6 3,928,794 - LRK53_RS17880 0.58 -1.5 3,928,794 - LRK53_RS17880 0.58 -0.9 3,928,794 - LRK53_RS17880 0.58 +0.2 3,928,794 - LRK53_RS17880 0.58 +1.0 3,928,794 - LRK53_RS17880 0.58 -0.8 3,928,794 - LRK53_RS17880 0.58 -0.6 3,928,794 - LRK53_RS17880 0.58 +1.0 3,928,794 - LRK53_RS17880 0.58 -0.0 3,928,794 - LRK53_RS17880 0.58 -0.3 3,928,794 - LRK53_RS17880 0.58 -0.0 3,928,794 - LRK53_RS17880 0.58 -0.2 3,928,836 - LRK53_RS17880 0.63 -0.0 3,928,836 - LRK53_RS17880 0.63 -3.1 3,928,836 - LRK53_RS17880 0.63 -1.0 3,928,904 + LRK53_RS17880 0.72 -0.6 3,928,904 + LRK53_RS17880 0.72 -1.0 3,928,904 + LRK53_RS17880 0.72 -1.3 3,928,905 - LRK53_RS17880 0.72 +0.4 3,928,905 - LRK53_RS17880 0.72 -1.3 3,928,905 - LRK53_RS17880 0.72 -0.1 3,928,923 - LRK53_RS17880 0.75 -1.0 3,928,923 - LRK53_RS17880 0.75 -1.2 3,928,949 + LRK53_RS17880 0.78 +1.0 3,929,146 + -1.7 3,929,146 + +0.4 3,929,146 + +1.0 3,929,146 + +1.0 3,929,146 + +1.0 3,929,146 + -1.8 3,929,146 + -1.0 3,929,146 + -1.5 3,929,146 + -0.0 3,929,146 + -1.1 3,929,146 + -0.4 3,929,147 - -0.7 3,929,147 - +2.0 3,929,147 - -1.0 3,929,147 - -1.3 3,929,147 - -0.7 3,929,147 - -0.0 3,929,147 - -0.3 3,929,147 - -2.3 3,929,147 - -0.6 3,929,147 - -0.6 3,929,147 - -0.6 3,929,147 - +0.3 3,929,147 - -0.0 3,929,147 - +0.4 3,929,147 - -0.0 3,929,147 - -0.7 3,929,147 - +0.7 3,929,147 - +0.1 3,929,147 - -1.2 3,929,147 - -1.1 3,929,205 + +1.0 3,929,205 + +1.0 3,929,205 + -0.7 3,929,205 + -1.3 3,929,205 + -0.1 3,929,205 + +0.4 3,929,205 + +1.0 3,929,206 - -0.0 3,929,206 - -0.9 3,929,206 - -1.8 3,929,206 - +1.0 3,929,206 - -0.3 3,929,206 - +1.0 3,929,206 - -1.6 3,929,224 + -0.4 3,929,283 - -0.0 3,929,283 - -0.0 3,929,369 + LRK53_RS17885 0.15 +1.0 3,929,369 + LRK53_RS17885 0.15 -0.0 3,929,369 + LRK53_RS17885 0.15 +1.0 3,929,370 - LRK53_RS17885 0.15 +1.0 3,929,486 + LRK53_RS17885 0.33 +1.0 3,929,486 + LRK53_RS17885 0.33 -0.0 3,929,486 + LRK53_RS17885 0.33 +1.0 3,929,561 + LRK53_RS17885 0.44 +1.0 3,929,891 + +1.0 3,929,981 + -0.8 3,929,981 + -0.7 3,929,981 + +0.2 3,929,981 + -1.0 3,929,981 + -2.2 3,929,981 + -0.0 3,929,982 - -1.3 3,929,982 - +1.0 3,929,982 - +0.4 3,929,982 - -0.9 3,929,982 - -0.6 3,930,003 + +0.3 3,930,003 + -0.0 3,930,003 + +1.0 3,930,004 - -1.6 3,930,004 - +0.4 3,930,004 - -0.6 3,930,004 - -0.6 3,930,004 - +1.0 3,930,005 + -2.5 3,930,005 + -0.6 3,930,005 + +0.6 3,930,005 + -2.7 3,930,005 + +1.0 3,930,005 + -0.1 3,930,005 + -1.3 3,930,005 + -0.6 3,930,005 + -1.7 3,930,005 + -0.6 3,930,006 - -0.1 3,930,006 - -1.3 3,930,006 - -0.6 3,930,006 - -0.0 3,930,006 - -0.2 3,930,006 - -0.6 3,930,006 - -0.0 3,930,006 - -0.4 3,930,006 - +1.0 3,930,025 + -1.1 3,930,025 + +1.0 3,930,025 + -0.0 3,930,114 + LRK53_RS17890 0.13 -1.1 3,930,114 + LRK53_RS17890 0.13 -0.8 3,930,114 + LRK53_RS17890 0.13 -0.0 3,930,114 + LRK53_RS17890 0.13 -0.0 3,930,114 + LRK53_RS17890 0.13 +1.0 3,930,114 + LRK53_RS17890 0.13 -0.6 3,930,115 - LRK53_RS17890 0.13 -0.6 3,930,115 - LRK53_RS17890 0.13 -0.3 3,930,115 - LRK53_RS17890 0.13 -1.9 3,930,115 - LRK53_RS17890 0.13 -0.6 3,930,115 - LRK53_RS17890 0.13 -0.5 3,930,115 - LRK53_RS17890 0.13 +2.0 3,930,115 - LRK53_RS17890 0.13 -0.8 3,930,115 - LRK53_RS17890 0.13 -0.6
Or see this region's nucleotide sequence