Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS17860 and LRK53_RS17865 are separated by 13 nucleotides LRK53_RS17865 and LRK53_RS17870 overlap by 31 nucleotides LRK53_RS17870 and LRK53_RS17875 are separated by 137 nucleotides
LRK53_RS17860: LRK53_RS17860 - peptidoglycan DD-metalloendopeptidase family protein, at 3,924,037 to 3,925,482
_RS17860
LRK53_RS17865: LRK53_RS17865 - anhydro-N-acetylmuramic acid kinase, at 3,925,496 to 3,926,626
_RS17865
LRK53_RS17870: LRK53_RS17870 - hypothetical protein, at 3,926,596 to 3,926,907
_RS17870
LRK53_RS17875: LRK53_RS17875 - MFS transporter, at 3,927,045 to 3,928,280
_RS17875
Position (kb)
3925
3926
3927 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3924.673 kb on + strand, within LRK53_RS17860 at 3924.740 kb on - strand, within LRK53_RS17860 at 3924.793 kb on + strand, within LRK53_RS17860 at 3924.794 kb on - strand, within LRK53_RS17860 at 3924.806 kb on - strand, within LRK53_RS17860 at 3924.919 kb on + strand, within LRK53_RS17860 at 3925.054 kb on + strand, within LRK53_RS17860 at 3925.054 kb on + strand, within LRK53_RS17860 at 3925.108 kb on + strand, within LRK53_RS17860 at 3925.108 kb on + strand, within LRK53_RS17860 at 3925.108 kb on + strand, within LRK53_RS17860 at 3925.109 kb on - strand, within LRK53_RS17860 at 3925.109 kb on - strand, within LRK53_RS17860 at 3925.109 kb on - strand, within LRK53_RS17860 at 3925.291 kb on + strand, within LRK53_RS17860 at 3925.517 kb on + strand at 3925.517 kb on + strand at 3925.517 kb on + strand at 3925.517 kb on + strand at 3925.517 kb on + strand at 3925.518 kb on - strand at 3926.198 kb on + strand, within LRK53_RS17865 at 3926.198 kb on + strand, within LRK53_RS17865 at 3926.198 kb on + strand, within LRK53_RS17865 at 3926.199 kb on - strand, within LRK53_RS17865 at 3926.199 kb on - strand, within LRK53_RS17865 at 3926.199 kb on - strand, within LRK53_RS17865 at 3926.237 kb on + strand, within LRK53_RS17865 at 3926.237 kb on + strand, within LRK53_RS17865 at 3926.238 kb on - strand, within LRK53_RS17865 at 3926.397 kb on + strand, within LRK53_RS17865 at 3926.597 kb on + strand at 3926.624 kb on + strand at 3926.625 kb on - strand at 3926.907 kb on + strand at 3926.907 kb on + strand at 3926.908 kb on - strand at 3926.908 kb on - strand at 3926.908 kb on - strand at 3926.908 kb on - strand at 3927.046 kb on + strand at 3927.047 kb on - strand at 3927.047 kb on - strand at 3927.155 kb on - strand at 3927.245 kb on - strand, within LRK53_RS17875 at 3927.245 kb on - strand, within LRK53_RS17875 at 3927.388 kb on + strand, within LRK53_RS17875 at 3927.388 kb on + strand, within LRK53_RS17875 at 3927.388 kb on + strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.389 kb on - strand, within LRK53_RS17875 at 3927.505 kb on + strand, within LRK53_RS17875 at 3927.506 kb on - strand, within LRK53_RS17875 at 3927.506 kb on - strand, within LRK53_RS17875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,924,673 + LRK53_RS17860 0.44 +0.4 3,924,740 - LRK53_RS17860 0.49 +1.0 3,924,793 + LRK53_RS17860 0.52 -0.3 3,924,794 - LRK53_RS17860 0.52 +0.4 3,924,806 - LRK53_RS17860 0.53 -1.8 3,924,919 + LRK53_RS17860 0.61 +1.0 3,925,054 + LRK53_RS17860 0.70 +1.0 3,925,054 + LRK53_RS17860 0.70 -1.0 3,925,108 + LRK53_RS17860 0.74 -1.6 3,925,108 + LRK53_RS17860 0.74 -0.6 3,925,108 + LRK53_RS17860 0.74 -0.6 3,925,109 - LRK53_RS17860 0.74 -0.0 3,925,109 - LRK53_RS17860 0.74 +0.4 3,925,109 - LRK53_RS17860 0.74 +2.0 3,925,291 + LRK53_RS17860 0.87 +1.0 3,925,517 + -0.0 3,925,517 + -0.0 3,925,517 + -1.2 3,925,517 + +2.0 3,925,517 + -0.6 3,925,518 - -0.6 3,926,198 + LRK53_RS17865 0.62 +1.0 3,926,198 + LRK53_RS17865 0.62 -0.6 3,926,198 + LRK53_RS17865 0.62 -1.8 3,926,199 - LRK53_RS17865 0.62 -0.0 3,926,199 - LRK53_RS17865 0.62 +0.4 3,926,199 - LRK53_RS17865 0.62 -1.0 3,926,237 + LRK53_RS17865 0.66 -0.0 3,926,237 + LRK53_RS17865 0.66 +1.0 3,926,238 - LRK53_RS17865 0.66 +1.0 3,926,397 + LRK53_RS17865 0.80 -0.0 3,926,597 + -2.0 3,926,624 + +1.0 3,926,625 - -0.3 3,926,907 + -0.2 3,926,907 + -0.0 3,926,908 - -0.7 3,926,908 - -0.3 3,926,908 - +0.7 3,926,908 - +1.6 3,927,046 + -0.6 3,927,047 - -0.8 3,927,047 - -0.6 3,927,155 - +1.0 3,927,245 - LRK53_RS17875 0.16 -0.0 3,927,245 - LRK53_RS17875 0.16 -1.0 3,927,388 + LRK53_RS17875 0.28 -0.0 3,927,388 + LRK53_RS17875 0.28 +1.0 3,927,388 + LRK53_RS17875 0.28 -1.3 3,927,389 - LRK53_RS17875 0.28 +1.0 3,927,389 - LRK53_RS17875 0.28 +2.0 3,927,389 - LRK53_RS17875 0.28 -0.0 3,927,389 - LRK53_RS17875 0.28 +0.3 3,927,505 + LRK53_RS17875 0.37 -0.6 3,927,506 - LRK53_RS17875 0.37 -0.8 3,927,506 - LRK53_RS17875 0.37 -0.0
Or see this region's nucleotide sequence