Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16780 and LRK53_RS16785 are separated by 78 nucleotides LRK53_RS16785 and LRK53_RS16790 overlap by 4 nucleotides LRK53_RS16790 and LRK53_RS16795 are separated by 88 nucleotides LRK53_RS16795 and LRK53_RS16800 are separated by 96 nucleotides
LRK53_RS16780: LRK53_RS16780 - ABC transporter ATP-binding protein, at 3,691,761 to 3,692,597
_RS16780
LRK53_RS16785: LRK53_RS16785 - guanylate kinase, at 3,692,676 to 3,693,305
_RS16785
LRK53_RS16790: LRK53_RS16790 - YicC family protein, at 3,693,302 to 3,694,162
_RS16790
LRK53_RS16795: LRK53_RS16795 - ribonuclease PH, at 3,694,251 to 3,694,979
_RS16795
LRK53_RS16800: LRK53_RS16800 - RdgB/HAM1 family non-canonical purine NTP pyrophosphatase, at 3,695,076 to 3,695,672
_RS16800
Position (kb)
3693
3694
3695 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.314 kb on + strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.315 kb on - strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.338 kb on + strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.339 kb on - strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.404 kb on + strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.405 kb on - strand, within LRK53_RS16780 at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.631 kb on + strand at 3692.632 kb on - strand at 3692.632 kb on - strand at 3692.632 kb on - strand at 3692.632 kb on - strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.658 kb on + strand at 3692.659 kb on - strand at 3692.659 kb on - strand at 3692.659 kb on - strand at 3692.659 kb on - strand at 3692.659 kb on - strand at 3692.659 kb on - strand at 3694.072 kb on - strand, within LRK53_RS16790 at 3694.072 kb on - strand, within LRK53_RS16790 at 3694.072 kb on - strand, within LRK53_RS16790 at 3694.140 kb on + strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.141 kb on - strand at 3694.198 kb on + strand at 3694.199 kb on - strand at 3694.199 kb on - strand at 3694.199 kb on - strand at 3694.199 kb on - strand at 3694.199 kb on - strand at 3694.982 kb on + strand at 3694.983 kb on - strand at 3694.983 kb on - strand at 3694.983 kb on - strand at 3694.983 kb on - strand at 3694.983 kb on - strand at 3694.983 kb on - strand at 3695.042 kb on + strand at 3695.042 kb on + strand at 3695.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,692,314 + LRK53_RS16780 0.66 -3.3 3,692,314 + LRK53_RS16780 0.66 -0.3 3,692,314 + LRK53_RS16780 0.66 -0.7 3,692,314 + LRK53_RS16780 0.66 -0.0 3,692,314 + LRK53_RS16780 0.66 -0.0 3,692,314 + LRK53_RS16780 0.66 -1.0 3,692,314 + LRK53_RS16780 0.66 -0.3 3,692,314 + LRK53_RS16780 0.66 +0.3 3,692,314 + LRK53_RS16780 0.66 -0.6 3,692,314 + LRK53_RS16780 0.66 +0.4 3,692,314 + LRK53_RS16780 0.66 +0.4 3,692,314 + LRK53_RS16780 0.66 -0.8 3,692,314 + LRK53_RS16780 0.66 -2.6 3,692,314 + LRK53_RS16780 0.66 -0.0 3,692,315 - LRK53_RS16780 0.66 -1.3 3,692,315 - LRK53_RS16780 0.66 -0.0 3,692,315 - LRK53_RS16780 0.66 -1.4 3,692,315 - LRK53_RS16780 0.66 +2.3 3,692,315 - LRK53_RS16780 0.66 -1.8 3,692,315 - LRK53_RS16780 0.66 -2.3 3,692,315 - LRK53_RS16780 0.66 -1.4 3,692,315 - LRK53_RS16780 0.66 -1.3 3,692,315 - LRK53_RS16780 0.66 -0.5 3,692,338 + LRK53_RS16780 0.69 -0.6 3,692,338 + LRK53_RS16780 0.69 -0.0 3,692,338 + LRK53_RS16780 0.69 -0.0 3,692,338 + LRK53_RS16780 0.69 +1.6 3,692,338 + LRK53_RS16780 0.69 +0.3 3,692,338 + LRK53_RS16780 0.69 -0.0 3,692,338 + LRK53_RS16780 0.69 +0.5 3,692,338 + LRK53_RS16780 0.69 +0.6 3,692,338 + LRK53_RS16780 0.69 +0.2 3,692,338 + LRK53_RS16780 0.69 +0.7 3,692,338 + LRK53_RS16780 0.69 -0.0 3,692,338 + LRK53_RS16780 0.69 +0.5 3,692,338 + LRK53_RS16780 0.69 +1.0 3,692,338 + LRK53_RS16780 0.69 -1.3 3,692,338 + LRK53_RS16780 0.69 -1.3 3,692,338 + LRK53_RS16780 0.69 -0.0 3,692,338 + LRK53_RS16780 0.69 +1.0 3,692,338 + LRK53_RS16780 0.69 -0.3 3,692,338 + LRK53_RS16780 0.69 -0.8 3,692,338 + LRK53_RS16780 0.69 -1.0 3,692,338 + LRK53_RS16780 0.69 -1.9 3,692,338 + LRK53_RS16780 0.69 -0.6 3,692,338 + LRK53_RS16780 0.69 -1.3 3,692,338 + LRK53_RS16780 0.69 +0.3 3,692,338 + LRK53_RS16780 0.69 -1.2 3,692,338 + LRK53_RS16780 0.69 +1.0 3,692,338 + LRK53_RS16780 0.69 -0.6 3,692,339 - LRK53_RS16780 0.69 -0.0 3,692,339 - LRK53_RS16780 0.69 -1.8 3,692,339 - LRK53_RS16780 0.69 +2.0 3,692,339 - LRK53_RS16780 0.69 +0.6 3,692,339 - LRK53_RS16780 0.69 +0.1 3,692,339 - LRK53_RS16780 0.69 -0.0 3,692,339 - LRK53_RS16780 0.69 +1.0 3,692,339 - LRK53_RS16780 0.69 +0.5 3,692,339 - LRK53_RS16780 0.69 -0.3 3,692,339 - LRK53_RS16780 0.69 -0.6 3,692,339 - LRK53_RS16780 0.69 -0.3 3,692,339 - LRK53_RS16780 0.69 +0.6 3,692,339 - LRK53_RS16780 0.69 -1.3 3,692,339 - LRK53_RS16780 0.69 +2.0 3,692,339 - LRK53_RS16780 0.69 -0.0 3,692,339 - LRK53_RS16780 0.69 -1.8 3,692,339 - LRK53_RS16780 0.69 -1.8 3,692,339 - LRK53_RS16780 0.69 +0.3 3,692,339 - LRK53_RS16780 0.69 -0.3 3,692,339 - LRK53_RS16780 0.69 -0.0 3,692,339 - LRK53_RS16780 0.69 -0.0 3,692,339 - LRK53_RS16780 0.69 -1.0 3,692,404 + LRK53_RS16780 0.77 -1.8 3,692,404 + LRK53_RS16780 0.77 -0.0 3,692,404 + LRK53_RS16780 0.77 +1.4 3,692,404 + LRK53_RS16780 0.77 -1.7 3,692,404 + LRK53_RS16780 0.77 +1.6 3,692,404 + LRK53_RS16780 0.77 -0.0 3,692,404 + LRK53_RS16780 0.77 -1.4 3,692,404 + LRK53_RS16780 0.77 +0.6 3,692,404 + LRK53_RS16780 0.77 -1.0 3,692,404 + LRK53_RS16780 0.77 -1.0 3,692,404 + LRK53_RS16780 0.77 -1.0 3,692,404 + LRK53_RS16780 0.77 -1.5 3,692,404 + LRK53_RS16780 0.77 -2.0 3,692,404 + LRK53_RS16780 0.77 -0.6 3,692,404 + LRK53_RS16780 0.77 -1.6 3,692,404 + LRK53_RS16780 0.77 -1.6 3,692,404 + LRK53_RS16780 0.77 -1.0 3,692,404 + LRK53_RS16780 0.77 -0.7 3,692,405 - LRK53_RS16780 0.77 -0.5 3,692,405 - LRK53_RS16780 0.77 -0.8 3,692,405 - LRK53_RS16780 0.77 -2.2 3,692,405 - LRK53_RS16780 0.77 -2.2 3,692,405 - LRK53_RS16780 0.77 -1.0 3,692,405 - LRK53_RS16780 0.77 -1.2 3,692,405 - LRK53_RS16780 0.77 -0.0 3,692,405 - LRK53_RS16780 0.77 -0.2 3,692,405 - LRK53_RS16780 0.77 -1.0 3,692,405 - LRK53_RS16780 0.77 -0.3 3,692,405 - LRK53_RS16780 0.77 -1.5 3,692,405 - LRK53_RS16780 0.77 -1.3 3,692,405 - LRK53_RS16780 0.77 -1.8 3,692,405 - LRK53_RS16780 0.77 +0.3 3,692,405 - LRK53_RS16780 0.77 -1.2 3,692,405 - LRK53_RS16780 0.77 -1.5 3,692,405 - LRK53_RS16780 0.77 -0.0 3,692,405 - LRK53_RS16780 0.77 -1.1 3,692,405 - LRK53_RS16780 0.77 -1.8 3,692,405 - LRK53_RS16780 0.77 -0.3 3,692,405 - LRK53_RS16780 0.77 -2.5 3,692,405 - LRK53_RS16780 0.77 -1.8 3,692,405 - LRK53_RS16780 0.77 -1.0 3,692,405 - LRK53_RS16780 0.77 -1.0 3,692,631 + +0.7 3,692,631 + -0.6 3,692,631 + +1.0 3,692,631 + -2.3 3,692,631 + -1.3 3,692,631 + -0.3 3,692,631 + +0.6 3,692,631 + -1.0 3,692,631 + -0.0 3,692,631 + +0.6 3,692,632 - +0.4 3,692,632 - -0.0 3,692,632 - -0.6 3,692,632 - -0.0 3,692,658 + -0.6 3,692,658 + +1.0 3,692,658 + -1.7 3,692,658 + -0.6 3,692,658 + +2.0 3,692,658 + -0.0 3,692,658 + -0.0 3,692,658 + -0.6 3,692,658 + -0.9 3,692,659 - -1.0 3,692,659 - -2.5 3,692,659 - -1.3 3,692,659 - -0.3 3,692,659 - +2.0 3,692,659 - +0.4 3,694,072 - LRK53_RS16790 0.89 -0.0 3,694,072 - LRK53_RS16790 0.89 -1.3 3,694,072 - LRK53_RS16790 0.89 -1.0 3,694,140 + +1.0 3,694,141 - -1.1 3,694,141 - -0.0 3,694,141 - +0.3 3,694,141 - -1.2 3,694,141 - +1.0 3,694,141 - -2.0 3,694,141 - -0.5 3,694,141 - -0.0 3,694,141 - -0.0 3,694,141 - -0.6 3,694,141 - -1.3 3,694,141 - -0.5 3,694,141 - -1.9 3,694,198 + +0.3 3,694,199 - +0.2 3,694,199 - -0.0 3,694,199 - +1.0 3,694,199 - -0.3 3,694,199 - -1.3 3,694,982 + -1.6 3,694,983 - -0.0 3,694,983 - -0.6 3,694,983 - +0.4 3,694,983 - +0.7 3,694,983 - +0.1 3,694,983 - -0.5 3,695,042 + -0.3 3,695,042 + -3.0 3,695,043 - -0.6
Or see this region's nucleotide sequence