Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16640 and LRK53_RS16645 are separated by 237 nucleotides LRK53_RS16645 and LRK53_RS16650 are separated by 60 nucleotides LRK53_RS16650 and LRK53_RS16655 are separated by 182 nucleotides
LRK53_RS16640: LRK53_RS16640 - hypothetical protein, at 3,661,696 to 3,662,148
_RS16640
LRK53_RS16645: LRK53_RS16645 - MarR family transcriptional regulator, at 3,662,386 to 3,662,841
_RS16645
LRK53_RS16650: LRK53_RS16650 - O-acetyl-ADP-ribose deacetylase, at 3,662,902 to 3,663,420
_RS16650
LRK53_RS16655: LRK53_RS16655 - BatD family protein, at 3,663,603 to 3,665,255
_RS16655
Position (kb)
3662
3663
3664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3661.982 kb on + strand, within LRK53_RS16640 at 3661.982 kb on + strand, within LRK53_RS16640 at 3661.982 kb on + strand, within LRK53_RS16640 at 3662.251 kb on + strand at 3662.251 kb on + strand at 3662.251 kb on + strand at 3662.251 kb on + strand at 3662.252 kb on - strand at 3662.252 kb on - strand at 3662.252 kb on - strand at 3662.252 kb on - strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.322 kb on + strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.323 kb on - strand at 3662.533 kb on + strand, within LRK53_RS16645 at 3662.533 kb on + strand, within LRK53_RS16645 at 3662.533 kb on + strand, within LRK53_RS16645 at 3662.561 kb on + strand, within LRK53_RS16645 at 3662.561 kb on + strand, within LRK53_RS16645 at 3662.561 kb on + strand, within LRK53_RS16645 at 3662.561 kb on + strand, within LRK53_RS16645 at 3662.561 kb on + strand, within LRK53_RS16645 at 3662.561 kb on + strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.562 kb on - strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.708 kb on + strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.709 kb on - strand, within LRK53_RS16645 at 3662.811 kb on - strand at 3662.811 kb on - strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.883 kb on + strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.884 kb on - strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.888 kb on + strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3662.889 kb on - strand at 3663.074 kb on + strand, within LRK53_RS16650 at 3663.074 kb on + strand, within LRK53_RS16650 at 3663.075 kb on - strand, within LRK53_RS16650 at 3663.075 kb on - strand, within LRK53_RS16650 at 3663.278 kb on - strand, within LRK53_RS16650 at 3663.418 kb on + strand at 3663.418 kb on + strand at 3663.418 kb on + strand at 3663.419 kb on - strand at 3663.419 kb on - strand at 3663.419 kb on - strand at 3663.419 kb on - strand at 3663.515 kb on + strand at 3663.515 kb on + strand at 3663.516 kb on - strand at 3663.622 kb on + strand at 3663.622 kb on + strand at 3663.622 kb on + strand at 3663.622 kb on + strand at 3663.622 kb on + strand at 3663.622 kb on + strand at 3663.622 kb on + strand at 3663.623 kb on - strand at 3663.623 kb on - strand at 3663.623 kb on - strand at 3663.623 kb on - strand at 3663.624 kb on + strand at 3663.625 kb on - strand at 3663.625 kb on - strand at 3663.625 kb on - strand at 3663.733 kb on + strand at 3663.733 kb on + strand at 3663.734 kb on - strand at 3663.734 kb on - strand at 3663.734 kb on - strand at 3663.734 kb on - strand at 3663.734 kb on - strand at 3663.734 kb on - strand at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.855 kb on + strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3663.856 kb on - strand, within LRK53_RS16655 at 3664.005 kb on + strand, within LRK53_RS16655 at 3664.005 kb on + strand, within LRK53_RS16655 at 3664.005 kb on + strand, within LRK53_RS16655 at 3664.006 kb on - strand, within LRK53_RS16655 at 3664.006 kb on - strand, within LRK53_RS16655 at 3664.143 kb on + strand, within LRK53_RS16655 at 3664.143 kb on + strand, within LRK53_RS16655 at 3664.143 kb on + strand, within LRK53_RS16655 at 3664.143 kb on + strand, within LRK53_RS16655 at 3664.143 kb on + strand, within LRK53_RS16655 at 3664.143 kb on + strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.144 kb on - strand, within LRK53_RS16655 at 3664.173 kb on + strand, within LRK53_RS16655 at 3664.173 kb on + strand, within LRK53_RS16655 at 3664.174 kb on - strand, within LRK53_RS16655 at 3664.174 kb on - strand, within LRK53_RS16655 at 3664.174 kb on - strand, within LRK53_RS16655 at 3664.330 kb on + strand, within LRK53_RS16655 at 3664.331 kb on - strand, within LRK53_RS16655 at 3664.337 kb on + strand, within LRK53_RS16655 at 3664.381 kb on + strand, within LRK53_RS16655 at 3664.381 kb on + strand, within LRK53_RS16655 at 3664.382 kb on - strand, within LRK53_RS16655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,661,982 + LRK53_RS16640 0.63 +0.8 3,661,982 + LRK53_RS16640 0.63 -0.6 3,661,982 + LRK53_RS16640 0.63 +0.7 3,662,251 + +0.6 3,662,251 + -0.6 3,662,251 + -2.3 3,662,251 + +0.0 3,662,252 - +0.2 3,662,252 - -1.1 3,662,252 - -1.6 3,662,252 - +0.4 3,662,322 + -0.3 3,662,322 + -1.6 3,662,322 + +2.0 3,662,322 + +0.0 3,662,322 + -0.3 3,662,322 + +0.0 3,662,322 + -0.5 3,662,322 + -1.5 3,662,323 - +1.0 3,662,323 - -1.3 3,662,323 - -1.1 3,662,323 - +0.0 3,662,323 - +0.5 3,662,323 - +1.0 3,662,323 - +0.0 3,662,323 - +0.0 3,662,533 + LRK53_RS16645 0.32 -0.2 3,662,533 + LRK53_RS16645 0.32 +0.0 3,662,533 + LRK53_RS16645 0.32 +0.0 3,662,561 + LRK53_RS16645 0.38 -0.7 3,662,561 + LRK53_RS16645 0.38 +0.4 3,662,561 + LRK53_RS16645 0.38 +0.0 3,662,561 + LRK53_RS16645 0.38 +0.4 3,662,561 + LRK53_RS16645 0.38 -1.6 3,662,561 + LRK53_RS16645 0.38 +1.0 3,662,562 - LRK53_RS16645 0.39 -0.1 3,662,562 - LRK53_RS16645 0.39 -2.9 3,662,562 - LRK53_RS16645 0.39 -0.6 3,662,562 - LRK53_RS16645 0.39 -1.3 3,662,562 - LRK53_RS16645 0.39 -0.3 3,662,562 - LRK53_RS16645 0.39 -0.9 3,662,562 - LRK53_RS16645 0.39 -0.4 3,662,562 - LRK53_RS16645 0.39 -0.1 3,662,562 - LRK53_RS16645 0.39 -0.4 3,662,562 - LRK53_RS16645 0.39 -1.1 3,662,562 - LRK53_RS16645 0.39 -0.3 3,662,562 - LRK53_RS16645 0.39 -0.4 3,662,562 - LRK53_RS16645 0.39 -1.0 3,662,562 - LRK53_RS16645 0.39 -3.0 3,662,562 - LRK53_RS16645 0.39 -0.2 3,662,562 - LRK53_RS16645 0.39 +0.1 3,662,562 - LRK53_RS16645 0.39 -0.2 3,662,708 + LRK53_RS16645 0.71 +1.0 3,662,708 + LRK53_RS16645 0.71 -0.4 3,662,708 + LRK53_RS16645 0.71 -1.0 3,662,708 + LRK53_RS16645 0.71 +0.0 3,662,708 + LRK53_RS16645 0.71 -1.8 3,662,708 + LRK53_RS16645 0.71 -1.0 3,662,708 + LRK53_RS16645 0.71 -0.4 3,662,708 + LRK53_RS16645 0.71 -0.6 3,662,708 + LRK53_RS16645 0.71 -1.4 3,662,709 - LRK53_RS16645 0.71 -0.8 3,662,709 - LRK53_RS16645 0.71 -0.5 3,662,709 - LRK53_RS16645 0.71 +0.0 3,662,709 - LRK53_RS16645 0.71 -0.4 3,662,709 - LRK53_RS16645 0.71 +0.0 3,662,709 - LRK53_RS16645 0.71 -1.2 3,662,709 - LRK53_RS16645 0.71 -0.7 3,662,709 - LRK53_RS16645 0.71 -1.2 3,662,709 - LRK53_RS16645 0.71 +0.0 3,662,709 - LRK53_RS16645 0.71 -1.0 3,662,709 - LRK53_RS16645 0.71 +1.0 3,662,811 - +0.0 3,662,811 - +1.0 3,662,883 + +0.5 3,662,883 + -0.3 3,662,883 + -1.5 3,662,883 + -1.3 3,662,883 + -2.2 3,662,883 + -0.6 3,662,883 + -0.2 3,662,883 + -0.6 3,662,884 - -0.6 3,662,884 - +1.0 3,662,884 - -0.3 3,662,884 - +2.0 3,662,884 - -1.0 3,662,884 - -1.5 3,662,884 - -0.1 3,662,884 - -1.8 3,662,884 - -0.7 3,662,884 - +1.0 3,662,884 - -0.4 3,662,888 + -0.8 3,662,888 + -1.3 3,662,888 + -2.6 3,662,888 + -0.5 3,662,888 + +1.0 3,662,888 + -1.0 3,662,888 + +0.4 3,662,888 + -1.3 3,662,888 + -1.1 3,662,888 + +1.0 3,662,888 + +1.3 3,662,888 + -0.5 3,662,888 + +3.6 3,662,888 + +0.8 3,662,888 + +1.0 3,662,888 + -0.6 3,662,888 + -0.3 3,662,888 + -1.5 3,662,888 + +0.6 3,662,888 + -1.1 3,662,888 + -0.4 3,662,889 - -0.8 3,662,889 - +2.0 3,662,889 - -0.6 3,662,889 - +0.0 3,662,889 - +0.0 3,662,889 - -0.6 3,662,889 - -0.8 3,662,889 - -1.3 3,662,889 - -0.8 3,662,889 - -0.3 3,662,889 - -0.6 3,662,889 - +0.4 3,662,889 - +0.0 3,662,889 - -1.3 3,662,889 - +1.0 3,662,889 - -1.1 3,662,889 - +2.0 3,662,889 - -0.2 3,662,889 - -3.3 3,662,889 - -1.0 3,662,889 - -0.9 3,662,889 - +1.0 3,662,889 - -0.3 3,662,889 - -2.2 3,662,889 - +0.0 3,662,889 - -3.0 3,662,889 - -0.2 3,663,074 + LRK53_RS16650 0.33 -1.0 3,663,074 + LRK53_RS16650 0.33 -1.0 3,663,075 - LRK53_RS16650 0.33 +0.4 3,663,075 - LRK53_RS16650 0.33 -3.7 3,663,278 - LRK53_RS16650 0.72 -1.3 3,663,418 + -2.3 3,663,418 + -0.8 3,663,418 + -1.5 3,663,419 - +0.0 3,663,419 - +0.0 3,663,419 - +0.0 3,663,419 - -0.4 3,663,515 + -1.4 3,663,515 + -0.2 3,663,516 - +0.0 3,663,622 + -0.3 3,663,622 + -0.6 3,663,622 + -1.8 3,663,622 + -1.0 3,663,622 + -0.3 3,663,622 + +0.0 3,663,622 + +0.0 3,663,623 - +1.0 3,663,623 - -1.4 3,663,623 - -1.8 3,663,623 - +0.0 3,663,624 + -0.3 3,663,625 - +1.6 3,663,625 - -1.1 3,663,625 - -0.4 3,663,733 + -0.3 3,663,733 + +0.0 3,663,734 - -0.2 3,663,734 - +0.0 3,663,734 - +1.0 3,663,734 - -0.6 3,663,734 - +0.6 3,663,734 - -1.3 3,663,855 + LRK53_RS16655 0.15 -1.0 3,663,855 + LRK53_RS16655 0.15 +0.0 3,663,855 + LRK53_RS16655 0.15 -2.9 3,663,855 + LRK53_RS16655 0.15 -0.2 3,663,855 + LRK53_RS16655 0.15 +0.0 3,663,855 + LRK53_RS16655 0.15 +0.0 3,663,855 + LRK53_RS16655 0.15 -0.7 3,663,855 + LRK53_RS16655 0.15 -0.6 3,663,855 + LRK53_RS16655 0.15 +1.0 3,663,855 + LRK53_RS16655 0.15 +1.0 3,663,855 + LRK53_RS16655 0.15 -1.0 3,663,855 + LRK53_RS16655 0.15 +0.6 3,663,855 + LRK53_RS16655 0.15 -0.4 3,663,855 + LRK53_RS16655 0.15 -0.8 3,663,856 - LRK53_RS16655 0.15 +0.7 3,663,856 - LRK53_RS16655 0.15 -1.0 3,663,856 - LRK53_RS16655 0.15 +0.0 3,663,856 - LRK53_RS16655 0.15 -2.1 3,663,856 - LRK53_RS16655 0.15 +0.4 3,663,856 - LRK53_RS16655 0.15 +0.0 3,663,856 - LRK53_RS16655 0.15 +1.0 3,663,856 - LRK53_RS16655 0.15 +0.0 3,663,856 - LRK53_RS16655 0.15 +0.0 3,663,856 - LRK53_RS16655 0.15 -2.3 3,663,856 - LRK53_RS16655 0.15 +0.0 3,663,856 - LRK53_RS16655 0.15 -1.2 3,664,005 + LRK53_RS16655 0.24 +0.0 3,664,005 + LRK53_RS16655 0.24 +0.0 3,664,005 + LRK53_RS16655 0.24 -0.2 3,664,006 - LRK53_RS16655 0.24 -0.6 3,664,006 - LRK53_RS16655 0.24 -1.0 3,664,143 + LRK53_RS16655 0.33 +0.4 3,664,143 + LRK53_RS16655 0.33 +0.4 3,664,143 + LRK53_RS16655 0.33 -0.8 3,664,143 + LRK53_RS16655 0.33 -1.0 3,664,143 + LRK53_RS16655 0.33 -0.2 3,664,143 + LRK53_RS16655 0.33 +0.3 3,664,144 - LRK53_RS16655 0.33 -0.2 3,664,144 - LRK53_RS16655 0.33 -1.0 3,664,144 - LRK53_RS16655 0.33 +0.5 3,664,144 - LRK53_RS16655 0.33 -0.6 3,664,144 - LRK53_RS16655 0.33 +0.3 3,664,144 - LRK53_RS16655 0.33 -0.8 3,664,144 - LRK53_RS16655 0.33 +1.0 3,664,173 + LRK53_RS16655 0.34 +1.0 3,664,173 + LRK53_RS16655 0.34 -0.6 3,664,174 - LRK53_RS16655 0.35 +0.4 3,664,174 - LRK53_RS16655 0.35 -0.6 3,664,174 - LRK53_RS16655 0.35 +2.0 3,664,330 + LRK53_RS16655 0.44 -1.0 3,664,331 - LRK53_RS16655 0.44 -1.6 3,664,337 + LRK53_RS16655 0.44 +1.0 3,664,381 + LRK53_RS16655 0.47 +0.4 3,664,381 + LRK53_RS16655 0.47 +0.0 3,664,382 - LRK53_RS16655 0.47 +0.3
Or see this region's nucleotide sequence