Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16515 and LRK53_RS16520 are separated by 297 nucleotides LRK53_RS16520 and LRK53_RS16525 overlap by 17 nucleotides LRK53_RS16525 and LRK53_RS16530 are separated by 308 nucleotides
LRK53_RS16515: LRK53_RS16515 - branched-chain amino acid transaminase, at 3,638,089 to 3,639,006
_RS16515
LRK53_RS16520: LRK53_RS16520 - HAMP domain-containing histidine kinase, at 3,639,304 to 3,640,587
_RS16520
LRK53_RS16525: LRK53_RS16525 - response regulator transcription factor, at 3,640,571 to 3,641,257
_RS16525
LRK53_RS16530: LRK53_RS16530 - 30S ribosomal protein S6--L-glutamate ligase, at 3,641,566 to 3,642,486
_RS16530
Position (kb)
3639
3640
3641 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.308 kb on + strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.309 kb on - strand, within LRK53_RS16515 at 3638.374 kb on + strand, within LRK53_RS16515 at 3638.374 kb on + strand, within LRK53_RS16515 at 3638.374 kb on + strand, within LRK53_RS16515 at 3638.374 kb on + strand, within LRK53_RS16515 at 3638.375 kb on - strand, within LRK53_RS16515 at 3638.375 kb on - strand, within LRK53_RS16515 at 3638.375 kb on - strand, within LRK53_RS16515 at 3638.375 kb on - strand, within LRK53_RS16515 at 3638.393 kb on - strand, within LRK53_RS16515 at 3638.470 kb on + strand, within LRK53_RS16515 at 3638.471 kb on - strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.575 kb on + strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.576 kb on - strand, within LRK53_RS16515 at 3638.817 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.818 kb on + strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.819 kb on - strand, within LRK53_RS16515 at 3638.822 kb on + strand, within LRK53_RS16515 at 3638.824 kb on + strand, within LRK53_RS16515 at 3638.824 kb on + strand, within LRK53_RS16515 at 3638.824 kb on + strand, within LRK53_RS16515 at 3638.824 kb on + strand, within LRK53_RS16515 at 3638.825 kb on - strand, within LRK53_RS16515 at 3638.825 kb on - strand, within LRK53_RS16515 at 3638.825 kb on - strand, within LRK53_RS16515 at 3638.825 kb on - strand, within LRK53_RS16515 at 3638.825 kb on - strand, within LRK53_RS16515 at 3638.825 kb on - strand, within LRK53_RS16515 at 3639.102 kb on + strand at 3639.102 kb on + strand at 3639.102 kb on + strand at 3639.103 kb on - strand at 3639.103 kb on - strand at 3639.103 kb on - strand at 3639.159 kb on + strand at 3639.159 kb on + strand at 3639.159 kb on + strand at 3639.159 kb on + strand at 3639.159 kb on + strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.160 kb on - strand at 3639.558 kb on - strand, within LRK53_RS16520 at 3639.766 kb on + strand, within LRK53_RS16520 at 3639.766 kb on + strand, within LRK53_RS16520 at 3639.766 kb on + strand, within LRK53_RS16520 at 3639.767 kb on - strand, within LRK53_RS16520 at 3640.215 kb on - strand, within LRK53_RS16520 at 3640.215 kb on - strand, within LRK53_RS16520 at 3640.215 kb on - strand, within LRK53_RS16520 at 3640.262 kb on + strand, within LRK53_RS16520 at 3640.263 kb on - strand, within LRK53_RS16520 at 3640.263 kb on - strand, within LRK53_RS16520 at 3640.346 kb on + strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.347 kb on - strand, within LRK53_RS16520 at 3640.572 kb on + strand at 3640.573 kb on - strand at 3640.573 kb on - strand at 3640.573 kb on - strand at 3640.573 kb on - strand at 3640.573 kb on - strand at 3641.306 kb on + strand at 3641.306 kb on + strand at 3641.306 kb on + strand at 3641.306 kb on + strand at 3641.307 kb on - strand at 3641.307 kb on - strand at 3641.307 kb on - strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.422 kb on + strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.423 kb on - strand at 3641.433 kb on + strand at 3641.494 kb on + strand at 3641.494 kb on + strand at 3641.494 kb on + strand at 3641.494 kb on + strand at 3641.494 kb on + strand at 3641.494 kb on + strand at 3641.495 kb on - strand at 3641.496 kb on + strand at 3641.496 kb on + strand at 3641.496 kb on + strand at 3641.497 kb on - strand at 3641.497 kb on - strand at 3641.497 kb on - strand at 3641.497 kb on - strand at 3641.512 kb on - strand at 3641.512 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,638,308 + LRK53_RS16515 0.24 -1.7 3,638,308 + LRK53_RS16515 0.24 +1.0 3,638,308 + LRK53_RS16515 0.24 +0.4 3,638,308 + LRK53_RS16515 0.24 -1.5 3,638,308 + LRK53_RS16515 0.24 -0.4 3,638,308 + LRK53_RS16515 0.24 +0.0 3,638,308 + LRK53_RS16515 0.24 -0.6 3,638,308 + LRK53_RS16515 0.24 -0.5 3,638,308 + LRK53_RS16515 0.24 -0.1 3,638,308 + LRK53_RS16515 0.24 +0.4 3,638,308 + LRK53_RS16515 0.24 -1.2 3,638,309 - LRK53_RS16515 0.24 +1.0 3,638,309 - LRK53_RS16515 0.24 -0.2 3,638,309 - LRK53_RS16515 0.24 -1.4 3,638,309 - LRK53_RS16515 0.24 +2.0 3,638,309 - LRK53_RS16515 0.24 +1.0 3,638,309 - LRK53_RS16515 0.24 +1.0 3,638,309 - LRK53_RS16515 0.24 +0.2 3,638,374 + LRK53_RS16515 0.31 -0.6 3,638,374 + LRK53_RS16515 0.31 -1.1 3,638,374 + LRK53_RS16515 0.31 +0.0 3,638,374 + LRK53_RS16515 0.31 -1.8 3,638,375 - LRK53_RS16515 0.31 +0.4 3,638,375 - LRK53_RS16515 0.31 +0.0 3,638,375 - LRK53_RS16515 0.31 +0.5 3,638,375 - LRK53_RS16515 0.31 -1.0 3,638,393 - LRK53_RS16515 0.33 -1.8 3,638,470 + LRK53_RS16515 0.42 -0.3 3,638,471 - LRK53_RS16515 0.42 +0.0 3,638,575 + LRK53_RS16515 0.53 -0.3 3,638,575 + LRK53_RS16515 0.53 +0.0 3,638,575 + LRK53_RS16515 0.53 -0.6 3,638,575 + LRK53_RS16515 0.53 -0.8 3,638,575 + LRK53_RS16515 0.53 +1.7 3,638,575 + LRK53_RS16515 0.53 +1.7 3,638,575 + LRK53_RS16515 0.53 +0.4 3,638,575 + LRK53_RS16515 0.53 -0.6 3,638,575 + LRK53_RS16515 0.53 -0.7 3,638,576 - LRK53_RS16515 0.53 -2.2 3,638,576 - LRK53_RS16515 0.53 +0.4 3,638,576 - LRK53_RS16515 0.53 -1.8 3,638,576 - LRK53_RS16515 0.53 +1.0 3,638,576 - LRK53_RS16515 0.53 -0.3 3,638,576 - LRK53_RS16515 0.53 +0.0 3,638,576 - LRK53_RS16515 0.53 -0.6 3,638,576 - LRK53_RS16515 0.53 -0.2 3,638,576 - LRK53_RS16515 0.53 -1.1 3,638,576 - LRK53_RS16515 0.53 +0.0 3,638,576 - LRK53_RS16515 0.53 -1.0 3,638,576 - LRK53_RS16515 0.53 -1.3 3,638,576 - LRK53_RS16515 0.53 -1.2 3,638,576 - LRK53_RS16515 0.53 +2.0 3,638,576 - LRK53_RS16515 0.53 +0.0 3,638,576 - LRK53_RS16515 0.53 -1.0 3,638,817 + LRK53_RS16515 0.79 +0.6 3,638,818 + LRK53_RS16515 0.79 -1.1 3,638,818 + LRK53_RS16515 0.79 -3.0 3,638,818 + LRK53_RS16515 0.79 -1.0 3,638,818 + LRK53_RS16515 0.79 -1.3 3,638,818 + LRK53_RS16515 0.79 -0.3 3,638,818 + LRK53_RS16515 0.79 -1.8 3,638,818 + LRK53_RS16515 0.79 +2.0 3,638,818 + LRK53_RS16515 0.79 +0.8 3,638,818 + LRK53_RS16515 0.79 -0.8 3,638,818 + LRK53_RS16515 0.79 +0.0 3,638,818 + LRK53_RS16515 0.79 +0.0 3,638,818 + LRK53_RS16515 0.79 +0.6 3,638,819 - LRK53_RS16515 0.80 -1.0 3,638,819 - LRK53_RS16515 0.80 -1.7 3,638,819 - LRK53_RS16515 0.80 +1.0 3,638,819 - LRK53_RS16515 0.80 -1.3 3,638,819 - LRK53_RS16515 0.80 -1.2 3,638,819 - LRK53_RS16515 0.80 +0.0 3,638,819 - LRK53_RS16515 0.80 -1.3 3,638,819 - LRK53_RS16515 0.80 -0.8 3,638,819 - LRK53_RS16515 0.80 -0.8 3,638,822 + LRK53_RS16515 0.80 -1.0 3,638,824 + LRK53_RS16515 0.80 -1.5 3,638,824 + LRK53_RS16515 0.80 -2.0 3,638,824 + LRK53_RS16515 0.80 +0.3 3,638,824 + LRK53_RS16515 0.80 -0.9 3,638,825 - LRK53_RS16515 0.80 -0.7 3,638,825 - LRK53_RS16515 0.80 -0.8 3,638,825 - LRK53_RS16515 0.80 -1.2 3,638,825 - LRK53_RS16515 0.80 +0.0 3,638,825 - LRK53_RS16515 0.80 -0.6 3,638,825 - LRK53_RS16515 0.80 -1.0 3,639,102 + +0.0 3,639,102 + -0.5 3,639,102 + -1.1 3,639,103 - +0.3 3,639,103 - -2.9 3,639,103 - -0.8 3,639,159 + -3.2 3,639,159 + +1.0 3,639,159 + -0.2 3,639,159 + -0.6 3,639,159 + -1.2 3,639,160 - -0.6 3,639,160 - +0.6 3,639,160 - -1.6 3,639,160 - -0.6 3,639,160 - -0.6 3,639,160 - -0.8 3,639,160 - -0.9 3,639,160 - -2.7 3,639,160 - +2.0 3,639,160 - -1.3 3,639,160 - +1.2 3,639,160 - -1.0 3,639,558 - LRK53_RS16520 0.20 +0.0 3,639,766 + LRK53_RS16520 0.36 +0.0 3,639,766 + LRK53_RS16520 0.36 -2.2 3,639,766 + LRK53_RS16520 0.36 +1.0 3,639,767 - LRK53_RS16520 0.36 +0.6 3,640,215 - LRK53_RS16520 0.71 -1.0 3,640,215 - LRK53_RS16520 0.71 -0.6 3,640,215 - LRK53_RS16520 0.71 -0.7 3,640,262 + LRK53_RS16520 0.75 -1.4 3,640,263 - LRK53_RS16520 0.75 -1.0 3,640,263 - LRK53_RS16520 0.75 +0.0 3,640,346 + LRK53_RS16520 0.81 -2.2 3,640,347 - LRK53_RS16520 0.81 +1.0 3,640,347 - LRK53_RS16520 0.81 +1.0 3,640,347 - LRK53_RS16520 0.81 -1.0 3,640,347 - LRK53_RS16520 0.81 +1.0 3,640,347 - LRK53_RS16520 0.81 +0.0 3,640,347 - LRK53_RS16520 0.81 -1.8 3,640,347 - LRK53_RS16520 0.81 -1.0 3,640,347 - LRK53_RS16520 0.81 -0.4 3,640,347 - LRK53_RS16520 0.81 -0.6 3,640,347 - LRK53_RS16520 0.81 +0.0 3,640,347 - LRK53_RS16520 0.81 -1.0 3,640,347 - LRK53_RS16520 0.81 -1.3 3,640,347 - LRK53_RS16520 0.81 -0.8 3,640,347 - LRK53_RS16520 0.81 +1.0 3,640,347 - LRK53_RS16520 0.81 +0.0 3,640,347 - LRK53_RS16520 0.81 -0.4 3,640,572 + +1.0 3,640,573 - -1.0 3,640,573 - +1.0 3,640,573 - -0.6 3,640,573 - -1.0 3,640,573 - +2.0 3,641,306 + +0.7 3,641,306 + -0.1 3,641,306 + +2.0 3,641,306 + -0.3 3,641,307 - +1.0 3,641,307 - -1.0 3,641,307 - -1.5 3,641,422 + -0.2 3,641,422 + +1.6 3,641,422 + -1.8 3,641,422 + -0.0 3,641,422 + -1.0 3,641,422 + -0.2 3,641,422 + -1.8 3,641,422 + -0.0 3,641,422 + +1.0 3,641,422 + +0.4 3,641,422 + -1.2 3,641,422 + +1.0 3,641,423 - +0.5 3,641,423 - -0.0 3,641,423 - -0.7 3,641,423 - -1.0 3,641,423 - +0.5 3,641,423 - +1.0 3,641,423 - +0.2 3,641,423 - +2.0 3,641,423 - -0.9 3,641,423 - -1.0 3,641,423 - +1.0 3,641,423 - -0.5 3,641,433 + -1.3 3,641,494 + -1.0 3,641,494 + +0.4 3,641,494 + +1.0 3,641,494 + -1.6 3,641,494 + -0.0 3,641,494 + -2.3 3,641,495 - -0.8 3,641,496 + +1.0 3,641,496 + -0.2 3,641,496 + +1.0 3,641,497 - +2.0 3,641,497 - -0.6 3,641,497 - +2.0 3,641,497 - -1.0 3,641,512 - +2.0 3,641,512 - +0.5
Or see this region's nucleotide sequence