Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16325 and LRK53_RS16330 are separated by 80 nucleotides LRK53_RS16330 and LRK53_RS16335 are separated by 26 nucleotides LRK53_RS16335 and LRK53_RS16340 are separated by 60 nucleotides LRK53_RS16340 and LRK53_RS16345 are separated by 19 nucleotides
LRK53_RS16325: LRK53_RS16325 - nucleoside permease, at 3,594,403 to 3,595,683
_RS16325
LRK53_RS16330: LRK53_RS16330 - TonB-dependent receptor plug domain-containing protein, at 3,595,764 to 3,596,378
_RS16330
LRK53_RS16335: LRK53_RS16335 - PepSY domain-containing protein, at 3,596,405 to 3,597,487
_RS16335
LRK53_RS16340: LRK53_RS16340 - CDP-alcohol phosphatidyltransferase family protein, at 3,597,548 to 3,598,180
_RS16340
LRK53_RS16345: LRK53_RS16345 - hypothetical protein, at 3,598,200 to 3,598,487
_RS16345
Position (kb)
3596
3597
3598 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.594 kb on + strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3595.595 kb on - strand at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.070 kb on + strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.071 kb on - strand, within LRK53_RS16330 at 3596.127 kb on + strand, within LRK53_RS16330 at 3596.128 kb on - strand, within LRK53_RS16330 at 3596.128 kb on - strand, within LRK53_RS16330 at 3596.325 kb on + strand at 3596.325 kb on + strand at 3596.325 kb on + strand at 3596.326 kb on - strand at 3596.486 kb on + strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.487 kb on - strand at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.687 kb on + strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.688 kb on - strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.826 kb on + strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3596.827 kb on - strand, within LRK53_RS16335 at 3597.029 kb on + strand, within LRK53_RS16335 at 3597.063 kb on + strand, within LRK53_RS16335 at 3597.518 kb on - strand at 3597.802 kb on + strand, within LRK53_RS16340 at 3597.802 kb on + strand, within LRK53_RS16340 at 3597.803 kb on - strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.821 kb on + strand, within LRK53_RS16340 at 3597.822 kb on - strand, within LRK53_RS16340 at 3597.822 kb on - strand, within LRK53_RS16340 at 3597.875 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.935 kb on + strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.936 kb on - strand, within LRK53_RS16340 at 3597.937 kb on + strand, within LRK53_RS16340 at 3597.938 kb on - strand, within LRK53_RS16340 at 3597.938 kb on - strand, within LRK53_RS16340 at 3598.093 kb on + strand, within LRK53_RS16340 at 3598.119 kb on - strand at 3598.192 kb on + strand at 3598.192 kb on + strand at 3598.192 kb on + strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.193 kb on - strand at 3598.201 kb on + strand at 3598.333 kb on - strand, within LRK53_RS16345 at 3598.333 kb on - strand, within LRK53_RS16345 at 3598.333 kb on - strand, within LRK53_RS16345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,595,594 + -0.0 3,595,594 + -0.5 3,595,594 + -0.4 3,595,594 + -1.8 3,595,594 + -1.8 3,595,594 + -0.0 3,595,594 + -0.8 3,595,594 + +1.0 3,595,594 + +0.4 3,595,594 + -0.7 3,595,594 + -0.0 3,595,594 + -1.2 3,595,594 + +0.2 3,595,594 + -0.9 3,595,594 + +2.0 3,595,594 + -0.3 3,595,594 + -1.2 3,595,594 + -0.0 3,595,595 - -0.9 3,595,595 - -0.6 3,595,595 - -0.4 3,595,595 - -0.5 3,595,595 - -0.6 3,595,595 - -3.1 3,595,595 - -0.6 3,595,595 - +2.0 3,595,595 - +0.7 3,595,595 - +1.0 3,595,595 - +0.4 3,595,595 - -0.3 3,595,595 - -0.9 3,595,595 - -1.0 3,595,595 - -0.9 3,595,595 - -1.8 3,596,070 + LRK53_RS16330 0.50 +1.6 3,596,070 + LRK53_RS16330 0.50 -2.2 3,596,070 + LRK53_RS16330 0.50 -2.8 3,596,070 + LRK53_RS16330 0.50 -1.3 3,596,070 + LRK53_RS16330 0.50 -2.2 3,596,070 + LRK53_RS16330 0.50 -0.0 3,596,070 + LRK53_RS16330 0.50 +0.4 3,596,070 + LRK53_RS16330 0.50 -1.0 3,596,070 + LRK53_RS16330 0.50 -1.0 3,596,070 + LRK53_RS16330 0.50 -1.8 3,596,070 + LRK53_RS16330 0.50 -0.6 3,596,070 + LRK53_RS16330 0.50 +1.0 3,596,071 - LRK53_RS16330 0.50 -1.0 3,596,071 - LRK53_RS16330 0.50 -0.8 3,596,071 - LRK53_RS16330 0.50 -3.3 3,596,071 - LRK53_RS16330 0.50 -4.3 3,596,071 - LRK53_RS16330 0.50 -2.1 3,596,071 - LRK53_RS16330 0.50 -1.0 3,596,071 - LRK53_RS16330 0.50 -1.9 3,596,071 - LRK53_RS16330 0.50 -0.0 3,596,071 - LRK53_RS16330 0.50 -2.0 3,596,071 - LRK53_RS16330 0.50 -3.1 3,596,071 - LRK53_RS16330 0.50 +0.3 3,596,071 - LRK53_RS16330 0.50 -0.0 3,596,071 - LRK53_RS16330 0.50 -3.0 3,596,071 - LRK53_RS16330 0.50 -1.5 3,596,071 - LRK53_RS16330 0.50 -3.9 3,596,071 - LRK53_RS16330 0.50 -2.7 3,596,071 - LRK53_RS16330 0.50 +0.4 3,596,127 + LRK53_RS16330 0.59 +1.0 3,596,128 - LRK53_RS16330 0.59 -2.2 3,596,128 - LRK53_RS16330 0.59 -2.6 3,596,325 + -0.4 3,596,325 + -1.3 3,596,325 + -0.0 3,596,326 - -1.0 3,596,486 + -1.6 3,596,487 - -1.0 3,596,487 - -2.8 3,596,487 - -1.3 3,596,487 - -1.9 3,596,487 - +1.0 3,596,487 - -1.6 3,596,487 - -1.6 3,596,487 - -3.1 3,596,687 + LRK53_RS16335 0.26 -1.3 3,596,687 + LRK53_RS16335 0.26 -0.7 3,596,687 + LRK53_RS16335 0.26 -0.6 3,596,687 + LRK53_RS16335 0.26 -2.5 3,596,687 + LRK53_RS16335 0.26 -1.8 3,596,688 - LRK53_RS16335 0.26 -2.3 3,596,688 - LRK53_RS16335 0.26 -1.6 3,596,688 - LRK53_RS16335 0.26 -2.8 3,596,688 - LRK53_RS16335 0.26 -0.6 3,596,688 - LRK53_RS16335 0.26 -1.6 3,596,688 - LRK53_RS16335 0.26 -2.9 3,596,688 - LRK53_RS16335 0.26 -4.5 3,596,826 + LRK53_RS16335 0.39 -0.6 3,596,826 + LRK53_RS16335 0.39 -2.7 3,596,826 + LRK53_RS16335 0.39 -0.6 3,596,826 + LRK53_RS16335 0.39 -2.0 3,596,827 - LRK53_RS16335 0.39 -2.6 3,596,827 - LRK53_RS16335 0.39 +1.0 3,596,827 - LRK53_RS16335 0.39 -1.6 3,596,827 - LRK53_RS16335 0.39 -1.3 3,596,827 - LRK53_RS16335 0.39 +1.0 3,596,827 - LRK53_RS16335 0.39 -0.6 3,597,029 + LRK53_RS16335 0.58 -2.0 3,597,063 + LRK53_RS16335 0.61 -2.8 3,597,518 - -1.8 3,597,802 + LRK53_RS16340 0.40 -0.0 3,597,802 + LRK53_RS16340 0.40 -0.6 3,597,803 - LRK53_RS16340 0.40 -0.6 3,597,821 + LRK53_RS16340 0.43 +1.0 3,597,821 + LRK53_RS16340 0.43 -1.1 3,597,821 + LRK53_RS16340 0.43 -0.8 3,597,821 + LRK53_RS16340 0.43 +0.3 3,597,821 + LRK53_RS16340 0.43 -0.0 3,597,822 - LRK53_RS16340 0.43 -1.3 3,597,822 - LRK53_RS16340 0.43 -1.3 3,597,875 + LRK53_RS16340 0.52 +0.4 3,597,935 + LRK53_RS16340 0.61 -0.0 3,597,935 + LRK53_RS16340 0.61 -1.8 3,597,935 + LRK53_RS16340 0.61 -2.0 3,597,935 + LRK53_RS16340 0.61 -0.6 3,597,935 + LRK53_RS16340 0.61 -1.3 3,597,935 + LRK53_RS16340 0.61 -1.8 3,597,935 + LRK53_RS16340 0.61 -1.8 3,597,935 + LRK53_RS16340 0.61 -0.9 3,597,935 + LRK53_RS16340 0.61 -0.3 3,597,935 + LRK53_RS16340 0.61 -1.8 3,597,936 - LRK53_RS16340 0.61 -0.4 3,597,936 - LRK53_RS16340 0.61 +0.6 3,597,936 - LRK53_RS16340 0.61 -0.0 3,597,936 - LRK53_RS16340 0.61 +0.4 3,597,936 - LRK53_RS16340 0.61 +1.0 3,597,936 - LRK53_RS16340 0.61 -0.0 3,597,936 - LRK53_RS16340 0.61 +1.0 3,597,936 - LRK53_RS16340 0.61 +2.6 3,597,936 - LRK53_RS16340 0.61 -0.8 3,597,936 - LRK53_RS16340 0.61 +0.6 3,597,936 - LRK53_RS16340 0.61 -0.6 3,597,936 - LRK53_RS16340 0.61 +0.7 3,597,936 - LRK53_RS16340 0.61 -0.7 3,597,936 - LRK53_RS16340 0.61 -1.5 3,597,937 + LRK53_RS16340 0.61 -1.0 3,597,938 - LRK53_RS16340 0.62 -1.0 3,597,938 - LRK53_RS16340 0.62 -1.7 3,598,093 + LRK53_RS16340 0.86 -1.6 3,598,119 - -0.3 3,598,192 + -1.0 3,598,192 + +1.0 3,598,192 + +0.4 3,598,193 - +0.4 3,598,193 - -1.3 3,598,193 - -0.5 3,598,193 - -0.0 3,598,193 - -2.5 3,598,201 + -0.6 3,598,333 - LRK53_RS16345 0.46 +0.6 3,598,333 - LRK53_RS16345 0.46 -1.6 3,598,333 - LRK53_RS16345 0.46 -1.0
Or see this region's nucleotide sequence