Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS16290 and LRK53_RS16300 are separated by 752 nucleotides LRK53_RS16300 and LRK53_RS16305 are separated by 344 nucleotides
LRK53_RS16290: LRK53_RS16290 - division/cell wall cluster transcriptional repressor MraZ, at 3,587,502 to 3,587,957
_RS16290
LRK53_RS16300: LRK53_RS16300 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 3,588,710 to 3,589,540
_RS16300
LRK53_RS16305: LRK53_RS16305 - penicillin-binding protein activator, at 3,589,885 to 3,591,816
_RS16305
Position (kb)
3588
3589
3590 Strain fitness (log2 ratio)
-2
-1
0
1 at 3587.798 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3587.837 kb on - strand, within LRK53_RS16290 at 3588.650 kb on + strand at 3588.650 kb on + strand at 3588.650 kb on + strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.651 kb on - strand at 3588.721 kb on - strand at 3588.721 kb on - strand at 3588.721 kb on - strand at 3588.721 kb on - strand at 3588.721 kb on - strand at 3588.721 kb on - strand at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.048 kb on - strand, within LRK53_RS16300 at 3589.520 kb on + strand at 3589.521 kb on - strand at 3589.521 kb on - strand at 3589.521 kb on - strand at 3589.521 kb on - strand at 3589.823 kb on - strand at 3589.843 kb on + strand at 3589.843 kb on + strand at 3589.844 kb on - strand at 3589.844 kb on - strand at 3590.095 kb on + strand, within LRK53_RS16305 at 3590.095 kb on + strand, within LRK53_RS16305 at 3590.407 kb on + strand, within LRK53_RS16305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,587,798 - LRK53_RS16290 0.65 -0.3 3,587,837 - LRK53_RS16290 0.73 -1.6 3,587,837 - LRK53_RS16290 0.73 +0.7 3,587,837 - LRK53_RS16290 0.73 -0.5 3,587,837 - LRK53_RS16290 0.73 -1.8 3,587,837 - LRK53_RS16290 0.73 -0.6 3,587,837 - LRK53_RS16290 0.73 -0.6 3,587,837 - LRK53_RS16290 0.73 -1.3 3,587,837 - LRK53_RS16290 0.73 -0.6 3,588,650 + +1.6 3,588,650 + -1.0 3,588,650 + -0.0 3,588,651 - +0.4 3,588,651 - +1.0 3,588,651 - -0.0 3,588,651 - -1.0 3,588,651 - -0.8 3,588,651 - +1.0 3,588,651 - -0.3 3,588,651 - -1.9 3,588,651 - +0.4 3,588,651 - -0.0 3,588,651 - +0.4 3,588,651 - +0.4 3,588,651 - -0.0 3,588,651 - -0.6 3,588,651 - -0.9 3,588,651 - -0.3 3,588,721 - +1.0 3,588,721 - +0.6 3,588,721 - -0.5 3,588,721 - -0.0 3,588,721 - -0.8 3,588,721 - -1.3 3,589,048 - LRK53_RS16300 0.41 -2.7 3,589,048 - LRK53_RS16300 0.41 -1.0 3,589,048 - LRK53_RS16300 0.41 -1.2 3,589,048 - LRK53_RS16300 0.41 -1.3 3,589,048 - LRK53_RS16300 0.41 +1.0 3,589,048 - LRK53_RS16300 0.41 -1.0 3,589,048 - LRK53_RS16300 0.41 +0.4 3,589,048 - LRK53_RS16300 0.41 -1.0 3,589,048 - LRK53_RS16300 0.41 -1.0 3,589,048 - LRK53_RS16300 0.41 -1.2 3,589,520 + -1.9 3,589,521 - -0.5 3,589,521 - -0.2 3,589,521 - -0.6 3,589,521 - -0.4 3,589,823 - +0.4 3,589,843 + -1.2 3,589,843 + +1.0 3,589,844 - -0.4 3,589,844 - +0.6 3,590,095 + LRK53_RS16305 0.11 -1.0 3,590,095 + LRK53_RS16305 0.11 -2.3 3,590,407 + LRK53_RS16305 0.27 -1.2
Or see this region's nucleotide sequence