Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS15855 and LRK53_RS15860 are separated by 18 nucleotides LRK53_RS15860 and LRK53_RS15865 are separated by 7 nucleotides LRK53_RS15865 and LRK53_RS15870 are separated by 85 nucleotides
LRK53_RS15855: LRK53_RS15855 - IS110 family transposase, at 3,466,960 to 3,467,982
_RS15855
LRK53_RS15860: LRK53_RS15860 - NAD-dependent epimerase/dehydratase family protein, at 3,468,001 to 3,468,840
_RS15860
LRK53_RS15865: LRK53_RS15865 - hypothetical protein, at 3,468,848 to 3,470,554
_RS15865
LRK53_RS15870: LRK53_RS15870 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,470,640 to 3,472,775
_RS15870
Position (kb)
3468
3469
3470
3471 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3467.980 kb on + strand at 3467.980 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3467.998 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.002 kb on + strand at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.328 kb on + strand, within LRK53_RS15860 at 3468.329 kb on - strand, within LRK53_RS15860 at 3468.329 kb on - strand, within LRK53_RS15860 at 3468.329 kb on - strand, within LRK53_RS15860 at 3468.329 kb on - strand, within LRK53_RS15860 at 3468.329 kb on - strand, within LRK53_RS15860 at 3468.329 kb on - strand, within LRK53_RS15860 at 3468.331 kb on - strand, within LRK53_RS15860 at 3468.833 kb on - strand at 3468.833 kb on - strand at 3469.197 kb on + strand, within LRK53_RS15865 at 3469.197 kb on + strand, within LRK53_RS15865 at 3469.198 kb on - strand, within LRK53_RS15865 at 3469.890 kb on + strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3469.891 kb on - strand, within LRK53_RS15865 at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.587 kb on + strand at 3470.588 kb on - strand at 3470.588 kb on - strand at 3470.588 kb on - strand at 3470.802 kb on - strand at 3470.881 kb on + strand, within LRK53_RS15870 at 3470.881 kb on + strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3470.882 kb on - strand, within LRK53_RS15870 at 3471.128 kb on + strand, within LRK53_RS15870 at 3471.410 kb on - strand, within LRK53_RS15870 at 3471.410 kb on - strand, within LRK53_RS15870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,467,980 + -1.0 3,467,980 + -1.8 3,467,998 + -1.0 3,467,998 + -1.9 3,467,998 + +0.4 3,467,998 + -1.0 3,467,998 + +1.4 3,467,998 + +1.0 3,467,998 + -1.6 3,467,998 + -0.0 3,467,998 + -0.0 3,467,998 + +1.0 3,467,998 + +0.4 3,467,998 + -2.0 3,467,998 + +0.4 3,467,998 + +0.4 3,468,002 + -1.2 3,468,002 + +1.0 3,468,002 + -1.3 3,468,002 + +3.6 3,468,002 + +1.6 3,468,002 + +1.0 3,468,002 + -1.0 3,468,002 + -2.2 3,468,002 + -0.6 3,468,002 + +0.6 3,468,002 + -0.6 3,468,002 + +1.0 3,468,328 + LRK53_RS15860 0.39 -0.2 3,468,328 + LRK53_RS15860 0.39 -0.6 3,468,328 + LRK53_RS15860 0.39 -2.0 3,468,328 + LRK53_RS15860 0.39 -1.3 3,468,328 + LRK53_RS15860 0.39 -1.5 3,468,328 + LRK53_RS15860 0.39 -0.3 3,468,328 + LRK53_RS15860 0.39 -0.3 3,468,328 + LRK53_RS15860 0.39 -0.0 3,468,328 + LRK53_RS15860 0.39 -0.6 3,468,328 + LRK53_RS15860 0.39 -0.3 3,468,328 + LRK53_RS15860 0.39 +1.0 3,468,328 + LRK53_RS15860 0.39 +0.2 3,468,328 + LRK53_RS15860 0.39 -0.8 3,468,328 + LRK53_RS15860 0.39 +0.1 3,468,328 + LRK53_RS15860 0.39 -0.0 3,468,328 + LRK53_RS15860 0.39 -1.2 3,468,329 - LRK53_RS15860 0.39 -1.0 3,468,329 - LRK53_RS15860 0.39 -0.2 3,468,329 - LRK53_RS15860 0.39 -0.5 3,468,329 - LRK53_RS15860 0.39 -0.5 3,468,329 - LRK53_RS15860 0.39 +0.4 3,468,329 - LRK53_RS15860 0.39 +0.7 3,468,331 - LRK53_RS15860 0.39 -0.0 3,468,833 - -0.9 3,468,833 - -2.0 3,469,197 + LRK53_RS15865 0.20 -1.0 3,469,197 + LRK53_RS15865 0.20 -0.6 3,469,198 - LRK53_RS15865 0.21 -1.3 3,469,890 + LRK53_RS15865 0.61 -1.0 3,469,891 - LRK53_RS15865 0.61 +0.2 3,469,891 - LRK53_RS15865 0.61 -0.9 3,469,891 - LRK53_RS15865 0.61 -1.3 3,469,891 - LRK53_RS15865 0.61 -2.0 3,470,587 + -1.3 3,470,587 + -3.3 3,470,587 + -0.0 3,470,587 + +1.0 3,470,587 + -1.1 3,470,587 + +2.6 3,470,588 - -1.6 3,470,588 - -1.3 3,470,588 - -1.8 3,470,802 - +1.0 3,470,881 + LRK53_RS15870 0.11 -0.0 3,470,881 + LRK53_RS15870 0.11 +0.2 3,470,882 - LRK53_RS15870 0.11 -0.6 3,470,882 - LRK53_RS15870 0.11 +1.0 3,470,882 - LRK53_RS15870 0.11 -0.0 3,471,128 + LRK53_RS15870 0.23 -0.6 3,471,410 - LRK53_RS15870 0.36 +1.2 3,471,410 - LRK53_RS15870 0.36 -2.6
Or see this region's nucleotide sequence