Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS14950 and LRK53_RS14955 overlap by 4 nucleotides LRK53_RS14955 and LRK53_RS14960 are separated by 16 nucleotides LRK53_RS14960 and LRK53_RS14965 overlap by 11 nucleotides LRK53_RS14965 and LRK53_RS14970 are separated by 37 nucleotides
LRK53_RS14950: LRK53_RS14950 - flagellar hook-length control protein FliK, at 3,270,305 to 3,271,447
_RS14950
LRK53_RS14955: LRK53_RS14955 - hypothetical protein, at 3,271,444 to 3,271,704
_RS14955
LRK53_RS14960: LRK53_RS14960 - DUF2802 domain-containing protein, at 3,271,721 to 3,272,068
_RS14960
LRK53_RS14965: LRK53_RS14965 - chemotaxis protein CheW, at 3,272,058 to 3,272,537
_RS14965
LRK53_RS14970: LRK53_RS14970 - flagellar motor protein MotD, at 3,272,575 to 3,273,585
_RS14970
Position (kb)
3272
3273 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3271.275 kb on + strand, within LRK53_RS14950 at 3271.275 kb on + strand, within LRK53_RS14950 at 3271.362 kb on + strand at 3271.362 kb on + strand at 3271.362 kb on + strand at 3271.363 kb on - strand at 3271.363 kb on - strand at 3271.459 kb on + strand at 3271.652 kb on - strand, within LRK53_RS14955 at 3271.684 kb on + strand at 3271.684 kb on + strand at 3271.685 kb on - strand at 3271.685 kb on - strand at 3271.839 kb on + strand, within LRK53_RS14960 at 3271.839 kb on + strand, within LRK53_RS14960 at 3271.840 kb on - strand, within LRK53_RS14960 at 3271.840 kb on - strand, within LRK53_RS14960 at 3272.376 kb on + strand, within LRK53_RS14965 at 3272.377 kb on - strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.464 kb on + strand, within LRK53_RS14965 at 3272.465 kb on - strand, within LRK53_RS14965 at 3272.465 kb on - strand, within LRK53_RS14965 at 3272.465 kb on - strand, within LRK53_RS14965 at 3272.465 kb on - strand, within LRK53_RS14965 at 3272.969 kb on + strand, within LRK53_RS14970 at 3272.970 kb on - strand, within LRK53_RS14970 at 3272.970 kb on - strand, within LRK53_RS14970 at 3273.485 kb on + strand at 3273.485 kb on + strand at 3273.485 kb on + strand at 3273.485 kb on + strand at 3273.485 kb on + strand at 3273.485 kb on + strand at 3273.485 kb on + strand at 3273.486 kb on - strand at 3273.486 kb on - strand at 3273.486 kb on - strand at 3273.486 kb on - strand at 3273.486 kb on - strand at 3273.486 kb on - strand at 3273.486 kb on - strand at 3273.530 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,271,275 + LRK53_RS14950 0.85 +0.1 3,271,275 + LRK53_RS14950 0.85 -1.7 3,271,362 + +0.4 3,271,362 + +1.0 3,271,362 + +1.0 3,271,363 - -1.6 3,271,363 - -0.2 3,271,459 + -0.4 3,271,652 - LRK53_RS14955 0.80 +1.0 3,271,684 + +1.0 3,271,684 + -1.0 3,271,685 - +0.4 3,271,685 - -0.6 3,271,839 + LRK53_RS14960 0.34 -0.0 3,271,839 + LRK53_RS14960 0.34 +1.0 3,271,840 - LRK53_RS14960 0.34 -0.7 3,271,840 - LRK53_RS14960 0.34 +0.6 3,272,376 + LRK53_RS14965 0.66 +1.0 3,272,377 - LRK53_RS14965 0.66 -1.0 3,272,464 + LRK53_RS14965 0.85 -1.6 3,272,464 + LRK53_RS14965 0.85 -1.8 3,272,464 + LRK53_RS14965 0.85 +2.0 3,272,464 + LRK53_RS14965 0.85 +0.4 3,272,464 + LRK53_RS14965 0.85 +0.4 3,272,464 + LRK53_RS14965 0.85 -1.7 3,272,464 + LRK53_RS14965 0.85 -0.6 3,272,464 + LRK53_RS14965 0.85 -0.6 3,272,464 + LRK53_RS14965 0.85 +2.0 3,272,464 + LRK53_RS14965 0.85 -1.5 3,272,464 + LRK53_RS14965 0.85 -0.6 3,272,464 + LRK53_RS14965 0.85 -0.0 3,272,464 + LRK53_RS14965 0.85 -0.7 3,272,464 + LRK53_RS14965 0.85 -1.0 3,272,465 - LRK53_RS14965 0.85 -0.0 3,272,465 - LRK53_RS14965 0.85 -2.6 3,272,465 - LRK53_RS14965 0.85 +1.0 3,272,465 - LRK53_RS14965 0.85 -0.3 3,272,969 + LRK53_RS14970 0.39 -1.0 3,272,970 - LRK53_RS14970 0.39 -0.6 3,272,970 - LRK53_RS14970 0.39 -1.6 3,273,485 + +0.2 3,273,485 + -1.8 3,273,485 + -0.0 3,273,485 + -0.0 3,273,485 + -0.3 3,273,485 + +0.4 3,273,485 + -1.0 3,273,486 - -0.2 3,273,486 - -0.2 3,273,486 - -1.8 3,273,486 - -2.2 3,273,486 - -0.1 3,273,486 - -1.5 3,273,486 - -0.2 3,273,530 + -0.3
Or see this region's nucleotide sequence