Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS14785 and LRK53_RS14790 overlap by 4 nucleotides LRK53_RS14790 and LRK53_RS14795 are separated by 58 nucleotides LRK53_RS14795 and LRK53_RS14800 are separated by 123 nucleotides
LRK53_RS14785: LRK53_RS14785 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 3,237,970 to 3,239,124
_RS14785
LRK53_RS14790: LRK53_RS14790 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 3,239,121 to 3,239,624
_RS14790
LRK53_RS14795: LRK53_RS14795 - hypothetical protein, at 3,239,683 to 3,239,952
_RS14795
LRK53_RS14800: LRK53_RS14800 - DUF1631 domain-containing protein, at 3,240,076 to 3,242,334
_RS14800
Position (kb)
3239
3240 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3238.269 kb on + strand, within LRK53_RS14785 at 3238.269 kb on + strand, within LRK53_RS14785 at 3238.270 kb on - strand, within LRK53_RS14785 at 3238.270 kb on - strand, within LRK53_RS14785 at 3238.270 kb on - strand, within LRK53_RS14785 at 3238.270 kb on - strand, within LRK53_RS14785 at 3238.369 kb on + strand, within LRK53_RS14785 at 3238.370 kb on - strand, within LRK53_RS14785 at 3238.379 kb on + strand, within LRK53_RS14785 at 3238.379 kb on + strand, within LRK53_RS14785 at 3238.677 kb on - strand, within LRK53_RS14785 at 3238.955 kb on + strand, within LRK53_RS14785 at 3238.956 kb on - strand, within LRK53_RS14785 at 3238.956 kb on - strand, within LRK53_RS14785 at 3239.092 kb on + strand at 3239.189 kb on + strand, within LRK53_RS14790 at 3239.189 kb on + strand, within LRK53_RS14790 at 3239.189 kb on + strand, within LRK53_RS14790 at 3239.464 kb on + strand, within LRK53_RS14790 at 3239.464 kb on + strand, within LRK53_RS14790 at 3239.464 kb on + strand, within LRK53_RS14790 at 3239.648 kb on - strand at 3239.648 kb on - strand at 3239.652 kb on + strand at 3239.652 kb on + strand at 3239.652 kb on + strand at 3239.652 kb on + strand at 3239.652 kb on + strand at 3239.653 kb on - strand at 3239.653 kb on - strand at 3239.653 kb on - strand at 3239.653 kb on - strand at 3239.653 kb on - strand at 3239.653 kb on - strand at 3239.653 kb on - strand at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.869 kb on + strand, within LRK53_RS14795 at 3239.870 kb on - strand, within LRK53_RS14795 at 3239.870 kb on - strand, within LRK53_RS14795 at 3239.870 kb on - strand, within LRK53_RS14795 at 3239.870 kb on - strand, within LRK53_RS14795 at 3239.870 kb on - strand, within LRK53_RS14795 at 3240.011 kb on + strand at 3240.054 kb on - strand at 3240.283 kb on + strand at 3240.283 kb on + strand at 3240.283 kb on + strand at 3240.283 kb on + strand at 3240.284 kb on - strand at 3240.284 kb on - strand at 3240.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,238,269 + LRK53_RS14785 0.26 -0.0 3,238,269 + LRK53_RS14785 0.26 -0.0 3,238,270 - LRK53_RS14785 0.26 +0.4 3,238,270 - LRK53_RS14785 0.26 -0.0 3,238,270 - LRK53_RS14785 0.26 +1.0 3,238,270 - LRK53_RS14785 0.26 +1.0 3,238,369 + LRK53_RS14785 0.35 +1.0 3,238,370 - LRK53_RS14785 0.35 -0.8 3,238,379 + LRK53_RS14785 0.35 -1.8 3,238,379 + LRK53_RS14785 0.35 +0.2 3,238,677 - LRK53_RS14785 0.61 +0.2 3,238,955 + LRK53_RS14785 0.85 -0.0 3,238,956 - LRK53_RS14785 0.85 -0.0 3,238,956 - LRK53_RS14785 0.85 -0.6 3,239,092 + +0.8 3,239,189 + LRK53_RS14790 0.13 -1.4 3,239,189 + LRK53_RS14790 0.13 -2.0 3,239,189 + LRK53_RS14790 0.13 -1.4 3,239,464 + LRK53_RS14790 0.68 -1.6 3,239,464 + LRK53_RS14790 0.68 -1.4 3,239,464 + LRK53_RS14790 0.68 -1.0 3,239,648 - -3.1 3,239,648 - -1.7 3,239,652 + -0.0 3,239,652 + -0.0 3,239,652 + -0.6 3,239,652 + -0.2 3,239,652 + -0.6 3,239,653 - -0.0 3,239,653 - -0.0 3,239,653 - -1.6 3,239,653 - +1.0 3,239,653 - -0.0 3,239,653 - -0.4 3,239,653 - +0.2 3,239,869 + LRK53_RS14795 0.69 -1.6 3,239,869 + LRK53_RS14795 0.69 +0.5 3,239,869 + LRK53_RS14795 0.69 -0.6 3,239,869 + LRK53_RS14795 0.69 -1.4 3,239,869 + LRK53_RS14795 0.69 +1.6 3,239,869 + LRK53_RS14795 0.69 -2.9 3,239,869 + LRK53_RS14795 0.69 -1.0 3,239,869 + LRK53_RS14795 0.69 -0.3 3,239,869 + LRK53_RS14795 0.69 -0.0 3,239,869 + LRK53_RS14795 0.69 -0.9 3,239,870 - LRK53_RS14795 0.69 -0.0 3,239,870 - LRK53_RS14795 0.69 -0.6 3,239,870 - LRK53_RS14795 0.69 -1.5 3,239,870 - LRK53_RS14795 0.69 +0.1 3,239,870 - LRK53_RS14795 0.69 -0.8 3,240,011 + -1.3 3,240,054 - +0.0 3,240,283 + -0.6 3,240,283 + -0.0 3,240,283 + -1.0 3,240,283 + -1.0 3,240,284 - -1.4 3,240,284 - +1.6 3,240,284 - -2.3
Or see this region's nucleotide sequence