Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS14760 and LRK53_RS14765 are separated by 99 nucleotides LRK53_RS14765 and LRK53_RS14770 are separated by 60 nucleotides LRK53_RS14770 and LRK53_RS14775 overlap by 4 nucleotides LRK53_RS14775 and LRK53_RS14780 are separated by 112 nucleotides
LRK53_RS14760: LRK53_RS14760 - lysophospholipid acyltransferase family protein, at 3,233,632 to 3,235,344
_RS14760
LRK53_RS14765: LRK53_RS14765 - YhgN family NAAT transporter, at 3,235,444 to 3,236,043
_RS14765
LRK53_RS14770: LRK53_RS14770 - SDR family NAD(P)-dependent oxidoreductase, at 3,236,104 to 3,236,874
_RS14770
LRK53_RS14775: LRK53_RS14775 - Grx4 family monothiol glutaredoxin, at 3,236,871 to 3,237,194
_RS14775
LRK53_RS14780: LRK53_RS14780 - superoxide dismutase, at 3,237,307 to 3,237,900
_RS14780
Position (kb)
3236
3237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3235.292 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.380 kb on + strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.381 kb on - strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.383 kb on + strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.384 kb on - strand at 3235.431 kb on + strand at 3235.431 kb on + strand at 3235.431 kb on + strand at 3235.431 kb on + strand at 3235.431 kb on + strand at 3235.431 kb on + strand at 3235.432 kb on - strand at 3235.432 kb on - strand at 3235.432 kb on - strand at 3235.432 kb on - strand at 3235.572 kb on - strand, within LRK53_RS14765 at 3236.097 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.166 kb on - strand at 3236.226 kb on - strand, within LRK53_RS14770 at 3236.226 kb on - strand, within LRK53_RS14770 at 3236.226 kb on - strand, within LRK53_RS14770 at 3236.687 kb on + strand, within LRK53_RS14770 at 3236.687 kb on + strand, within LRK53_RS14770 at 3236.687 kb on + strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3236.688 kb on - strand, within LRK53_RS14770 at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.214 kb on - strand at 3237.237 kb on + strand at 3237.237 kb on + strand at 3237.237 kb on + strand at 3237.237 kb on + strand at 3237.237 kb on + strand at 3237.237 kb on + strand at 3237.237 kb on + strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand at 3237.238 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 3,235,292 + +2.0 3,235,380 + -0.0 3,235,380 + -0.2 3,235,380 + -0.6 3,235,380 + -1.0 3,235,380 + -0.8 3,235,380 + -0.5 3,235,380 + -0.0 3,235,380 + +2.5 3,235,381 - +1.0 3,235,381 - -0.0 3,235,381 - +0.4 3,235,381 - -0.0 3,235,381 - +1.0 3,235,381 - +0.3 3,235,381 - -0.3 3,235,381 - -0.9 3,235,381 - -0.0 3,235,381 - -1.7 3,235,381 - -0.8 3,235,381 - -0.8 3,235,381 - -0.0 3,235,381 - -0.5 3,235,381 - -2.7 3,235,381 - -0.0 3,235,381 - -2.4 3,235,383 + -1.0 3,235,383 + -0.9 3,235,383 + -0.6 3,235,383 + +0.4 3,235,383 + +1.5 3,235,383 + +1.0 3,235,383 + -3.0 3,235,383 + -1.0 3,235,383 + +0.4 3,235,384 - -0.6 3,235,384 - -1.1 3,235,384 - +2.0 3,235,384 - -0.6 3,235,384 - -1.5 3,235,384 - -1.6 3,235,384 - -0.4 3,235,384 - -1.4 3,235,384 - +0.2 3,235,384 - -0.1 3,235,384 - -2.0 3,235,431 + -0.8 3,235,431 + -0.6 3,235,431 + -0.0 3,235,431 + +1.0 3,235,431 + -1.8 3,235,431 + -0.6 3,235,432 - -0.4 3,235,432 - -1.8 3,235,432 - +0.2 3,235,432 - +0.3 3,235,572 - LRK53_RS14765 0.21 -0.0 3,236,097 - -1.7 3,236,166 - -1.0 3,236,166 - -1.4 3,236,166 - -2.1 3,236,166 - -1.2 3,236,166 - -0.0 3,236,166 - -0.6 3,236,166 - -0.6 3,236,166 - -0.0 3,236,166 - -1.4 3,236,166 - -0.4 3,236,166 - -0.0 3,236,166 - -1.0 3,236,166 - -1.6 3,236,226 - LRK53_RS14770 0.16 -1.0 3,236,226 - LRK53_RS14770 0.16 -0.6 3,236,226 - LRK53_RS14770 0.16 -0.6 3,236,687 + LRK53_RS14770 0.76 -0.0 3,236,687 + LRK53_RS14770 0.76 -0.0 3,236,687 + LRK53_RS14770 0.76 +1.0 3,236,688 - LRK53_RS14770 0.76 +1.0 3,236,688 - LRK53_RS14770 0.76 -1.8 3,236,688 - LRK53_RS14770 0.76 -1.4 3,236,688 - LRK53_RS14770 0.76 -2.5 3,236,688 - LRK53_RS14770 0.76 -1.0 3,236,688 - LRK53_RS14770 0.76 -1.0 3,236,688 - LRK53_RS14770 0.76 +1.0 3,236,688 - LRK53_RS14770 0.76 +0.4 3,236,688 - LRK53_RS14770 0.76 -1.6 3,236,688 - LRK53_RS14770 0.76 -1.6 3,236,688 - LRK53_RS14770 0.76 +1.0 3,236,688 - LRK53_RS14770 0.76 -0.6 3,236,688 - LRK53_RS14770 0.76 +1.0 3,237,214 - +1.0 3,237,214 - +0.5 3,237,214 - +1.0 3,237,214 - -1.0 3,237,214 - -1.4 3,237,214 - -1.8 3,237,214 - -1.0 3,237,214 - -0.4 3,237,214 - -0.0 3,237,214 - -1.0 3,237,214 - +2.5 3,237,237 + -0.0 3,237,237 + -2.0 3,237,237 + -0.6 3,237,237 + -1.6 3,237,237 + -1.6 3,237,237 + -1.8 3,237,237 + +0.2 3,237,238 - -0.0 3,237,238 - -1.2 3,237,238 - -0.8 3,237,238 - -0.2 3,237,238 - -0.4 3,237,238 - +1.0 3,237,238 - +0.1 3,237,238 - -1.5 3,237,238 - -0.6 3,237,238 - -1.0 3,237,238 - -0.8
Or see this region's nucleotide sequence