Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS13440 and LRK53_RS13445 overlap by 4 nucleotides LRK53_RS13445 and LRK53_RS13450 are separated by 26 nucleotides LRK53_RS13450 and LRK53_RS13455 are separated by 154 nucleotides LRK53_RS13455 and LRK53_RS13460 are separated by 40 nucleotides
LRK53_RS13440: LRK53_RS13440 - sulfurtransferase, at 2,969,429 to 2,970,277
_RS13440
LRK53_RS13445: LRK53_RS13445 - DUF1289 domain-containing protein, at 2,970,274 to 2,971,055
_RS13445
LRK53_RS13450: LRK53_RS13450 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 2,971,082 to 2,971,816
_RS13450
LRK53_RS13455: LRK53_RS13455 - enoyl-CoA hydratase-related protein, at 2,971,971 to 2,972,753
_RS13455
LRK53_RS13460: LRK53_RS13460 - DUF1684 domain-containing protein, at 2,972,794 to 2,973,699
_RS13460
Position (kb)
2971
2972 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2970.159 kb on + strand, within LRK53_RS13440 at 2970.159 kb on + strand, within LRK53_RS13440 at 2970.160 kb on - strand, within LRK53_RS13440 at 2970.160 kb on - strand, within LRK53_RS13440 at 2970.160 kb on - strand, within LRK53_RS13440 at 2970.160 kb on - strand, within LRK53_RS13440 at 2970.160 kb on - strand, within LRK53_RS13440 at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.305 kb on + strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.306 kb on - strand at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.393 kb on + strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.394 kb on - strand, within LRK53_RS13445 at 2970.395 kb on + strand, within LRK53_RS13445 at 2970.395 kb on + strand, within LRK53_RS13445 at 2970.396 kb on - strand, within LRK53_RS13445 at 2970.396 kb on - strand, within LRK53_RS13445 at 2970.396 kb on - strand, within LRK53_RS13445 at 2970.473 kb on + strand, within LRK53_RS13445 at 2970.522 kb on - strand, within LRK53_RS13445 at 2970.522 kb on - strand, within LRK53_RS13445 at 2970.551 kb on + strand, within LRK53_RS13445 at 2970.552 kb on - strand, within LRK53_RS13445 at 2970.552 kb on - strand, within LRK53_RS13445 at 2970.976 kb on + strand, within LRK53_RS13445 at 2970.976 kb on + strand, within LRK53_RS13445 at 2970.977 kb on - strand at 2971.068 kb on + strand at 2971.068 kb on + strand at 2971.068 kb on + strand at 2971.069 kb on - strand at 2971.069 kb on - strand at 2971.069 kb on - strand at 2971.069 kb on - strand at 2971.083 kb on + strand at 2971.083 kb on + strand at 2971.083 kb on + strand at 2971.084 kb on - strand at 2971.084 kb on - strand at 2971.084 kb on - strand at 2971.084 kb on - strand at 2971.084 kb on - strand at 2971.341 kb on + strand, within LRK53_RS13450 at 2971.341 kb on + strand, within LRK53_RS13450 at 2971.341 kb on + strand, within LRK53_RS13450 at 2971.342 kb on - strand, within LRK53_RS13450 at 2971.342 kb on - strand, within LRK53_RS13450 at 2971.342 kb on - strand, within LRK53_RS13450 at 2971.342 kb on - strand, within LRK53_RS13450 at 2971.407 kb on + strand, within LRK53_RS13450 at 2971.407 kb on + strand, within LRK53_RS13450 at 2971.408 kb on - strand, within LRK53_RS13450 at 2971.408 kb on - strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.464 kb on + strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.465 kb on - strand, within LRK53_RS13450 at 2971.599 kb on + strand, within LRK53_RS13450 at 2971.599 kb on + strand, within LRK53_RS13450 at 2971.599 kb on + strand, within LRK53_RS13450 at 2971.599 kb on + strand, within LRK53_RS13450 at 2971.600 kb on - strand, within LRK53_RS13450 at 2971.689 kb on + strand, within LRK53_RS13450 at 2971.859 kb on + strand at 2971.859 kb on + strand at 2971.859 kb on + strand at 2971.860 kb on - strand at 2971.860 kb on - strand at 2971.860 kb on - strand at 2971.935 kb on + strand at 2971.935 kb on + strand at 2971.935 kb on + strand at 2971.936 kb on - strand at 2971.936 kb on - strand at 2971.936 kb on - strand at 2971.977 kb on + strand at 2972.190 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.192 kb on + strand, within LRK53_RS13455 at 2972.193 kb on - strand, within LRK53_RS13455 at 2972.193 kb on - strand, within LRK53_RS13455 at 2972.193 kb on - strand, within LRK53_RS13455 at 2972.193 kb on - strand, within LRK53_RS13455 at 2972.193 kb on - strand, within LRK53_RS13455 at 2972.193 kb on - strand, within LRK53_RS13455 at 2972.490 kb on + strand, within LRK53_RS13455 at 2972.490 kb on + strand, within LRK53_RS13455 at 2972.490 kb on + strand, within LRK53_RS13455 at 2972.490 kb on + strand, within LRK53_RS13455 at 2972.491 kb on - strand, within LRK53_RS13455 at 2972.491 kb on - strand, within LRK53_RS13455 at 2972.491 kb on - strand, within LRK53_RS13455 at 2972.491 kb on - strand, within LRK53_RS13455 at 2972.810 kb on + strand at 2972.810 kb on + strand at 2972.810 kb on + strand at 2972.810 kb on + strand at 2972.810 kb on + strand at 2972.810 kb on + strand at 2972.811 kb on - strand at 2972.811 kb on - strand at 2972.811 kb on - strand at 2972.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,970,159 + LRK53_RS13440 0.86 +1.0 2,970,159 + LRK53_RS13440 0.86 +0.6 2,970,160 - LRK53_RS13440 0.86 -0.6 2,970,160 - LRK53_RS13440 0.86 +1.0 2,970,160 - LRK53_RS13440 0.86 -1.6 2,970,160 - LRK53_RS13440 0.86 -0.0 2,970,160 - LRK53_RS13440 0.86 -0.5 2,970,305 + -1.2 2,970,305 + -0.9 2,970,305 + -1.2 2,970,305 + -1.3 2,970,305 + -1.5 2,970,305 + -1.3 2,970,305 + -1.6 2,970,305 + -2.1 2,970,305 + -0.0 2,970,305 + -0.2 2,970,305 + -0.7 2,970,305 + -0.0 2,970,306 - +1.3 2,970,306 - +1.3 2,970,306 - -0.7 2,970,306 - -0.5 2,970,306 - -0.0 2,970,306 - -0.6 2,970,306 - +2.6 2,970,306 - -0.6 2,970,306 - +2.0 2,970,306 - -0.3 2,970,306 - -1.0 2,970,393 + LRK53_RS13445 0.15 -0.0 2,970,393 + LRK53_RS13445 0.15 +0.2 2,970,393 + LRK53_RS13445 0.15 -0.6 2,970,393 + LRK53_RS13445 0.15 -1.0 2,970,393 + LRK53_RS13445 0.15 -1.3 2,970,393 + LRK53_RS13445 0.15 +0.2 2,970,393 + LRK53_RS13445 0.15 -1.1 2,970,393 + LRK53_RS13445 0.15 -0.2 2,970,393 + LRK53_RS13445 0.15 +1.0 2,970,394 - LRK53_RS13445 0.15 -0.0 2,970,394 - LRK53_RS13445 0.15 +0.4 2,970,394 - LRK53_RS13445 0.15 -0.0 2,970,394 - LRK53_RS13445 0.15 -0.3 2,970,394 - LRK53_RS13445 0.15 -1.3 2,970,394 - LRK53_RS13445 0.15 -0.3 2,970,394 - LRK53_RS13445 0.15 -1.3 2,970,394 - LRK53_RS13445 0.15 -1.3 2,970,394 - LRK53_RS13445 0.15 +0.8 2,970,394 - LRK53_RS13445 0.15 -1.2 2,970,394 - LRK53_RS13445 0.15 -0.7 2,970,394 - LRK53_RS13445 0.15 -0.0 2,970,395 + LRK53_RS13445 0.15 -0.6 2,970,395 + LRK53_RS13445 0.15 -0.3 2,970,396 - LRK53_RS13445 0.16 +1.0 2,970,396 - LRK53_RS13445 0.16 -1.1 2,970,396 - LRK53_RS13445 0.16 -1.3 2,970,473 + LRK53_RS13445 0.25 +0.4 2,970,522 - LRK53_RS13445 0.32 +0.4 2,970,522 - LRK53_RS13445 0.32 +0.3 2,970,551 + LRK53_RS13445 0.35 -0.0 2,970,552 - LRK53_RS13445 0.36 -0.3 2,970,552 - LRK53_RS13445 0.36 -2.3 2,970,976 + LRK53_RS13445 0.90 +2.0 2,970,976 + LRK53_RS13445 0.90 -0.5 2,970,977 - -0.0 2,971,068 + -1.4 2,971,068 + -1.0 2,971,068 + -0.5 2,971,069 - -2.5 2,971,069 - +0.2 2,971,069 - +0.3 2,971,069 - +1.0 2,971,083 + -0.8 2,971,083 + -0.2 2,971,083 + +0.3 2,971,084 - -1.5 2,971,084 - -1.3 2,971,084 - +0.4 2,971,084 - -1.2 2,971,084 - -0.6 2,971,341 + LRK53_RS13450 0.35 -0.6 2,971,341 + LRK53_RS13450 0.35 -0.6 2,971,341 + LRK53_RS13450 0.35 -0.0 2,971,342 - LRK53_RS13450 0.35 -0.0 2,971,342 - LRK53_RS13450 0.35 +1.0 2,971,342 - LRK53_RS13450 0.35 -0.6 2,971,342 - LRK53_RS13450 0.35 -0.0 2,971,407 + LRK53_RS13450 0.44 -1.3 2,971,407 + LRK53_RS13450 0.44 +0.4 2,971,408 - LRK53_RS13450 0.44 -2.0 2,971,408 - LRK53_RS13450 0.44 -2.8 2,971,464 + LRK53_RS13450 0.52 -0.3 2,971,464 + LRK53_RS13450 0.52 -0.2 2,971,464 + LRK53_RS13450 0.52 +0.8 2,971,464 + LRK53_RS13450 0.52 -0.0 2,971,464 + LRK53_RS13450 0.52 +0.6 2,971,464 + LRK53_RS13450 0.52 -0.1 2,971,464 + LRK53_RS13450 0.52 -0.0 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 -2.3 2,971,464 + LRK53_RS13450 0.52 -0.6 2,971,464 + LRK53_RS13450 0.52 -2.7 2,971,464 + LRK53_RS13450 0.52 -1.0 2,971,464 + LRK53_RS13450 0.52 -1.3 2,971,464 + LRK53_RS13450 0.52 -0.0 2,971,464 + LRK53_RS13450 0.52 -1.0 2,971,464 + LRK53_RS13450 0.52 -1.2 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 -0.0 2,971,464 + LRK53_RS13450 0.52 -0.6 2,971,464 + LRK53_RS13450 0.52 -0.6 2,971,464 + LRK53_RS13450 0.52 -0.0 2,971,464 + LRK53_RS13450 0.52 -0.1 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 +2.0 2,971,464 + LRK53_RS13450 0.52 -1.3 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 -1.6 2,971,464 + LRK53_RS13450 0.52 -1.0 2,971,464 + LRK53_RS13450 0.52 -1.1 2,971,464 + LRK53_RS13450 0.52 +1.0 2,971,464 + LRK53_RS13450 0.52 +0.2 2,971,464 + LRK53_RS13450 0.52 -1.0 2,971,465 - LRK53_RS13450 0.52 -1.3 2,971,465 - LRK53_RS13450 0.52 -0.2 2,971,465 - LRK53_RS13450 0.52 +1.0 2,971,465 - LRK53_RS13450 0.52 -1.3 2,971,465 - LRK53_RS13450 0.52 +1.0 2,971,465 - LRK53_RS13450 0.52 -1.1 2,971,465 - LRK53_RS13450 0.52 +1.0 2,971,465 - LRK53_RS13450 0.52 -0.6 2,971,465 - LRK53_RS13450 0.52 -0.0 2,971,465 - LRK53_RS13450 0.52 -0.6 2,971,465 - LRK53_RS13450 0.52 -0.0 2,971,465 - LRK53_RS13450 0.52 -1.5 2,971,465 - LRK53_RS13450 0.52 -0.0 2,971,465 - LRK53_RS13450 0.52 +1.0 2,971,465 - LRK53_RS13450 0.52 +1.0 2,971,465 - LRK53_RS13450 0.52 -1.0 2,971,465 - LRK53_RS13450 0.52 -0.0 2,971,465 - LRK53_RS13450 0.52 -1.3 2,971,465 - LRK53_RS13450 0.52 +3.0 2,971,599 + LRK53_RS13450 0.70 -0.0 2,971,599 + LRK53_RS13450 0.70 -0.0 2,971,599 + LRK53_RS13450 0.70 -1.6 2,971,599 + LRK53_RS13450 0.70 -0.6 2,971,600 - LRK53_RS13450 0.70 -0.0 2,971,689 + LRK53_RS13450 0.83 +2.6 2,971,859 + -0.0 2,971,859 + +0.6 2,971,859 + -0.0 2,971,860 - -0.0 2,971,860 - -1.3 2,971,860 - -0.0 2,971,935 + -0.1 2,971,935 + -0.2 2,971,935 + -2.2 2,971,936 - -0.4 2,971,936 - -0.0 2,971,936 - +1.2 2,971,977 + +0.7 2,972,190 + LRK53_RS13455 0.28 -1.1 2,972,192 + LRK53_RS13455 0.28 -1.3 2,972,192 + LRK53_RS13455 0.28 -1.0 2,972,192 + LRK53_RS13455 0.28 -1.8 2,972,192 + LRK53_RS13455 0.28 -1.3 2,972,192 + LRK53_RS13455 0.28 -0.3 2,972,192 + LRK53_RS13455 0.28 +1.0 2,972,192 + LRK53_RS13455 0.28 -0.6 2,972,192 + LRK53_RS13455 0.28 +0.3 2,972,192 + LRK53_RS13455 0.28 -0.6 2,972,192 + LRK53_RS13455 0.28 -1.3 2,972,193 - LRK53_RS13455 0.28 -0.0 2,972,193 - LRK53_RS13455 0.28 -0.8 2,972,193 - LRK53_RS13455 0.28 -2.2 2,972,193 - LRK53_RS13455 0.28 +1.0 2,972,193 - LRK53_RS13455 0.28 -1.2 2,972,193 - LRK53_RS13455 0.28 -0.1 2,972,490 + LRK53_RS13455 0.66 +1.0 2,972,490 + LRK53_RS13455 0.66 -1.5 2,972,490 + LRK53_RS13455 0.66 -0.4 2,972,490 + LRK53_RS13455 0.66 -0.9 2,972,491 - LRK53_RS13455 0.66 +0.8 2,972,491 - LRK53_RS13455 0.66 -2.0 2,972,491 - LRK53_RS13455 0.66 -1.4 2,972,491 - LRK53_RS13455 0.66 -0.6 2,972,810 + -0.6 2,972,810 + -0.3 2,972,810 + +0.3 2,972,810 + +0.6 2,972,810 + +0.2 2,972,810 + +1.0 2,972,811 - +1.0 2,972,811 - +1.0 2,972,811 - -1.3 2,972,811 - -1.1
Or see this region's nucleotide sequence