Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS13170 and LRK53_RS13175 are separated by 118 nucleotides LRK53_RS13175 and LRK53_RS13180 are separated by 39 nucleotides LRK53_RS13180 and LRK53_RS13185 are separated by 338 nucleotides
LRK53_RS13170: LRK53_RS13170 - protoglobin domain-containing protein, at 2,903,141 to 2,903,737
_RS13170
LRK53_RS13175: LRK53_RS13175 - winged helix-turn-helix transcriptional regulator, at 2,903,856 to 2,904,263
_RS13175
LRK53_RS13180: LRK53_RS13180 - hypothetical protein, at 2,904,303 to 2,904,608
_RS13180
LRK53_RS13185: LRK53_RS13185 - NAD(P)H-dependent oxidoreductase, at 2,904,947 to 2,905,516
_RS13185
Position (kb)
2904
2905 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2903.394 kb on + strand, within LRK53_RS13170 at 2903.395 kb on - strand, within LRK53_RS13170 at 2903.416 kb on - strand, within LRK53_RS13170 at 2903.476 kb on - strand, within LRK53_RS13170 at 2903.476 kb on - strand, within LRK53_RS13170 at 2903.551 kb on - strand, within LRK53_RS13170 at 2903.610 kb on + strand, within LRK53_RS13170 at 2903.610 kb on + strand, within LRK53_RS13170 at 2903.610 kb on + strand, within LRK53_RS13170 at 2903.610 kb on + strand, within LRK53_RS13170 at 2903.610 kb on + strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.611 kb on - strand, within LRK53_RS13170 at 2903.709 kb on + strand at 2903.709 kb on + strand at 2903.709 kb on + strand at 2903.709 kb on + strand at 2903.709 kb on + strand at 2903.710 kb on - strand at 2903.710 kb on - strand at 2903.801 kb on + strand at 2903.802 kb on - strand at 2903.802 kb on - strand at 2903.850 kb on + strand at 2904.525 kb on - strand, within LRK53_RS13180 at 2904.553 kb on + strand, within LRK53_RS13180 at 2904.553 kb on + strand, within LRK53_RS13180 at 2904.554 kb on - strand, within LRK53_RS13180 at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.617 kb on + strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.618 kb on - strand at 2904.696 kb on - strand at 2904.749 kb on + strand at 2904.750 kb on - strand at 2904.750 kb on - strand at 2905.205 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.281 kb on + strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.282 kb on - strand, within LRK53_RS13185 at 2905.383 kb on + strand, within LRK53_RS13185 at 2905.383 kb on + strand, within LRK53_RS13185 at 2905.383 kb on + strand, within LRK53_RS13185 at 2905.384 kb on - strand, within LRK53_RS13185 at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.540 kb on + strand at 2905.541 kb on - strand at 2905.541 kb on - strand at 2905.541 kb on - strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.552 kb on + strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand at 2905.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,903,394 + LRK53_RS13170 0.42 -0.9 2,903,395 - LRK53_RS13170 0.43 -0.6 2,903,416 - LRK53_RS13170 0.46 -0.4 2,903,476 - LRK53_RS13170 0.56 -0.0 2,903,476 - LRK53_RS13170 0.56 -0.4 2,903,551 - LRK53_RS13170 0.69 -0.4 2,903,610 + LRK53_RS13170 0.79 +0.4 2,903,610 + LRK53_RS13170 0.79 -2.2 2,903,610 + LRK53_RS13170 0.79 +1.0 2,903,610 + LRK53_RS13170 0.79 -1.0 2,903,610 + LRK53_RS13170 0.79 +0.2 2,903,611 - LRK53_RS13170 0.79 -3.3 2,903,611 - LRK53_RS13170 0.79 -0.7 2,903,611 - LRK53_RS13170 0.79 -0.6 2,903,611 - LRK53_RS13170 0.79 +0.2 2,903,611 - LRK53_RS13170 0.79 -1.0 2,903,611 - LRK53_RS13170 0.79 +1.0 2,903,611 - LRK53_RS13170 0.79 -0.8 2,903,709 + -1.6 2,903,709 + -0.0 2,903,709 + -0.8 2,903,709 + +0.4 2,903,709 + +0.4 2,903,710 - -1.0 2,903,710 - -0.7 2,903,801 + -1.0 2,903,802 - -0.4 2,903,802 - -0.0 2,903,850 + -0.6 2,904,525 - LRK53_RS13180 0.73 -1.6 2,904,553 + LRK53_RS13180 0.82 -2.4 2,904,553 + LRK53_RS13180 0.82 -0.4 2,904,554 - LRK53_RS13180 0.82 -1.0 2,904,617 + -1.1 2,904,617 + -0.6 2,904,617 + +1.0 2,904,617 + -1.0 2,904,617 + -1.8 2,904,617 + -0.6 2,904,617 + -0.2 2,904,617 + -1.6 2,904,617 + -2.5 2,904,617 + -1.8 2,904,617 + +0.2 2,904,617 + +0.4 2,904,617 + -1.4 2,904,617 + -0.8 2,904,617 + +0.4 2,904,617 + +1.0 2,904,617 + -2.4 2,904,617 + -0.6 2,904,617 + -0.0 2,904,617 + +1.0 2,904,617 + -0.6 2,904,617 + +1.2 2,904,617 + +0.5 2,904,617 + -0.6 2,904,617 + -1.0 2,904,617 + -0.4 2,904,617 + -1.4 2,904,617 + -1.6 2,904,617 + -0.4 2,904,617 + +1.4 2,904,617 + -1.0 2,904,617 + -1.0 2,904,617 + -1.8 2,904,618 - -1.0 2,904,618 - -0.6 2,904,618 - +0.2 2,904,618 - +1.0 2,904,618 - -0.2 2,904,618 - +0.6 2,904,618 - -0.7 2,904,618 - -1.2 2,904,618 - +1.0 2,904,618 - -0.6 2,904,618 - +0.4 2,904,618 - -2.4 2,904,618 - -0.0 2,904,618 - +1.0 2,904,618 - -0.6 2,904,618 - -0.0 2,904,618 - -2.5 2,904,618 - -0.0 2,904,618 - +2.6 2,904,618 - -0.0 2,904,618 - +0.5 2,904,618 - -1.1 2,904,618 - -0.8 2,904,618 - -0.6 2,904,618 - -0.4 2,904,618 - -0.5 2,904,618 - -0.0 2,904,618 - -0.0 2,904,618 - -0.8 2,904,618 - -1.0 2,904,618 - -1.6 2,904,618 - -0.0 2,904,618 - -0.0 2,904,618 - -1.7 2,904,618 - -0.5 2,904,618 - -2.7 2,904,696 - -3.6 2,904,749 + -1.2 2,904,750 - +1.0 2,904,750 - -0.6 2,905,205 + LRK53_RS13185 0.45 +0.2 2,905,281 + LRK53_RS13185 0.59 +0.1 2,905,281 + LRK53_RS13185 0.59 +1.4 2,905,281 + LRK53_RS13185 0.59 -1.0 2,905,281 + LRK53_RS13185 0.59 -1.0 2,905,282 - LRK53_RS13185 0.59 -1.0 2,905,282 - LRK53_RS13185 0.59 -0.8 2,905,282 - LRK53_RS13185 0.59 -1.4 2,905,282 - LRK53_RS13185 0.59 -1.0 2,905,282 - LRK53_RS13185 0.59 -1.0 2,905,282 - LRK53_RS13185 0.59 -1.6 2,905,282 - LRK53_RS13185 0.59 -1.8 2,905,282 - LRK53_RS13185 0.59 -0.0 2,905,282 - LRK53_RS13185 0.59 +0.7 2,905,282 - LRK53_RS13185 0.59 -1.6 2,905,383 + LRK53_RS13185 0.76 -1.1 2,905,383 + LRK53_RS13185 0.76 -0.6 2,905,383 + LRK53_RS13185 0.76 +0.4 2,905,384 - LRK53_RS13185 0.77 -0.3 2,905,540 + -0.4 2,905,540 + -1.3 2,905,540 + -0.0 2,905,540 + -1.8 2,905,540 + -0.0 2,905,540 + -0.6 2,905,541 - -1.8 2,905,541 - +0.2 2,905,541 - -1.8 2,905,552 + -1.2 2,905,552 + -0.6 2,905,552 + +1.6 2,905,552 + +1.0 2,905,552 + -0.7 2,905,552 + +0.6 2,905,552 + -1.4 2,905,553 - +1.2 2,905,553 - -0.7 2,905,553 - -0.8 2,905,553 - +1.0 2,905,553 - -0.4 2,905,553 - -0.0 2,905,553 - -1.0 2,905,553 - -1.1
Or see this region's nucleotide sequence