Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12575 and LRK53_RS12580 overlap by 4 nucleotides LRK53_RS12580 and LRK53_RS12585 are separated by 9 nucleotides LRK53_RS12585 and LRK53_RS12590 are separated by 92 nucleotides
LRK53_RS12575: LRK53_RS12575 - lipid A export permease/ATP-binding protein MsbA, at 2,785,017 to 2,786,801
_RS12575
LRK53_RS12580: LRK53_RS12580 - biopolymer transporter ExbD, at 2,786,798 to 2,787,229
_RS12580
LRK53_RS12585: LRK53_RS12585 - MotA/TolQ/ExbB proton channel family protein, at 2,787,239 to 2,787,901
_RS12585
LRK53_RS12590: LRK53_RS12590 - DNA internalization-related competence protein ComEC/Rec2, at 2,787,994 to 2,790,294
_RS12590
Position (kb)
2787
2788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2787.016 kb on - strand, within LRK53_RS12580 at 2787.016 kb on - strand, within LRK53_RS12580 at 2787.016 kb on - strand, within LRK53_RS12580 at 2787.016 kb on - strand, within LRK53_RS12580 at 2787.016 kb on - strand, within LRK53_RS12580 at 2787.058 kb on + strand, within LRK53_RS12580 at 2787.059 kb on - strand, within LRK53_RS12580 at 2787.059 kb on - strand, within LRK53_RS12580 at 2787.350 kb on + strand, within LRK53_RS12585 at 2787.350 kb on + strand, within LRK53_RS12585 at 2787.350 kb on + strand, within LRK53_RS12585 at 2787.350 kb on + strand, within LRK53_RS12585 at 2787.350 kb on + strand, within LRK53_RS12585 at 2787.351 kb on - strand, within LRK53_RS12585 at 2787.382 kb on - strand, within LRK53_RS12585 at 2787.382 kb on - strand, within LRK53_RS12585 at 2787.382 kb on - strand, within LRK53_RS12585 at 2787.397 kb on - strand, within LRK53_RS12585 at 2787.397 kb on - strand, within LRK53_RS12585 at 2787.397 kb on - strand, within LRK53_RS12585 at 2787.397 kb on - strand, within LRK53_RS12585 at 2787.586 kb on - strand, within LRK53_RS12585 at 2787.942 kb on + strand at 2787.942 kb on + strand at 2787.942 kb on + strand at 2787.943 kb on - strand at 2787.943 kb on - strand at 2787.943 kb on - strand at 2787.961 kb on + strand at 2787.962 kb on - strand at 2787.962 kb on - strand at 2787.962 kb on - strand at 2788.007 kb on + strand at 2788.662 kb on - strand, within LRK53_RS12590 at 2788.679 kb on + strand, within LRK53_RS12590 at 2788.679 kb on + strand, within LRK53_RS12590 at 2788.679 kb on + strand, within LRK53_RS12590 at 2788.679 kb on + strand, within LRK53_RS12590 at 2788.679 kb on + strand, within LRK53_RS12590 at 2788.679 kb on + strand, within LRK53_RS12590 at 2788.680 kb on - strand, within LRK53_RS12590 at 2788.680 kb on - strand, within LRK53_RS12590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,787,016 - LRK53_RS12580 0.50 +1.6 2,787,016 - LRK53_RS12580 0.50 +0.2 2,787,016 - LRK53_RS12580 0.50 -1.6 2,787,016 - LRK53_RS12580 0.50 -2.2 2,787,016 - LRK53_RS12580 0.50 -2.2 2,787,058 + LRK53_RS12580 0.60 +2.0 2,787,059 - LRK53_RS12580 0.60 -1.5 2,787,059 - LRK53_RS12580 0.60 -1.8 2,787,350 + LRK53_RS12585 0.17 -1.0 2,787,350 + LRK53_RS12585 0.17 -1.8 2,787,350 + LRK53_RS12585 0.17 -0.0 2,787,350 + LRK53_RS12585 0.17 -1.8 2,787,350 + LRK53_RS12585 0.17 -0.0 2,787,351 - LRK53_RS12585 0.17 -0.6 2,787,382 - LRK53_RS12585 0.22 -0.6 2,787,382 - LRK53_RS12585 0.22 -1.6 2,787,382 - LRK53_RS12585 0.22 -1.0 2,787,397 - LRK53_RS12585 0.24 -2.0 2,787,397 - LRK53_RS12585 0.24 -1.4 2,787,397 - LRK53_RS12585 0.24 -1.3 2,787,397 - LRK53_RS12585 0.24 -1.6 2,787,586 - LRK53_RS12585 0.52 +1.2 2,787,942 + -0.0 2,787,942 + +1.0 2,787,942 + -1.2 2,787,943 - -0.6 2,787,943 - -1.1 2,787,943 - -1.0 2,787,961 + -2.0 2,787,962 - +0.3 2,787,962 - -0.0 2,787,962 - +0.4 2,788,007 + -0.7 2,788,662 - LRK53_RS12590 0.29 -1.4 2,788,679 + LRK53_RS12590 0.30 -0.0 2,788,679 + LRK53_RS12590 0.30 +0.4 2,788,679 + LRK53_RS12590 0.30 -1.3 2,788,679 + LRK53_RS12590 0.30 -0.8 2,788,679 + LRK53_RS12590 0.30 +1.0 2,788,679 + LRK53_RS12590 0.30 +0.2 2,788,680 - LRK53_RS12590 0.30 -0.0 2,788,680 - LRK53_RS12590 0.30 -0.8
Or see this region's nucleotide sequence