Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12365 and LRK53_RS12370 are separated by 112 nucleotides LRK53_RS12370 and LRK53_RS12375 are separated by 90 nucleotides
LRK53_RS12365: LRK53_RS12365 - patatin-like phospholipase family protein, at 2,745,361 to 2,746,245
_RS12365
LRK53_RS12370: LRK53_RS12370 - VOC family protein, at 2,746,358 to 2,746,786
_RS12370
LRK53_RS12375: LRK53_RS12375 - acetyl-CoA hydrolase/transferase family protein, at 2,746,877 to 2,748,385
_RS12375
Position (kb)
2746
2747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2745.476 kb on + strand, within LRK53_RS12365 at 2745.476 kb on + strand, within LRK53_RS12365 at 2745.482 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.560 kb on + strand, within LRK53_RS12365 at 2745.561 kb on - strand, within LRK53_RS12365 at 2745.561 kb on - strand, within LRK53_RS12365 at 2745.561 kb on - strand, within LRK53_RS12365 at 2745.561 kb on - strand, within LRK53_RS12365 at 2745.764 kb on + strand, within LRK53_RS12365 at 2745.865 kb on + strand, within LRK53_RS12365 at 2745.865 kb on + strand, within LRK53_RS12365 at 2745.865 kb on + strand, within LRK53_RS12365 at 2745.865 kb on + strand, within LRK53_RS12365 at 2746.073 kb on + strand, within LRK53_RS12365 at 2746.073 kb on + strand, within LRK53_RS12365 at 2746.073 kb on + strand, within LRK53_RS12365 at 2746.074 kb on - strand, within LRK53_RS12365 at 2746.074 kb on - strand, within LRK53_RS12365 at 2746.074 kb on - strand, within LRK53_RS12365 at 2746.074 kb on - strand, within LRK53_RS12365 at 2746.269 kb on - strand at 2746.269 kb on - strand at 2746.269 kb on - strand at 2746.569 kb on + strand, within LRK53_RS12370 at 2746.729 kb on - strand, within LRK53_RS12370 at 2746.729 kb on - strand, within LRK53_RS12370 at 2746.771 kb on - strand at 2746.771 kb on - strand at 2746.771 kb on - strand at 2746.903 kb on - strand at 2746.956 kb on + strand at 2746.956 kb on + strand at 2746.957 kb on - strand at 2746.957 kb on - strand at 2746.957 kb on - strand at 2746.957 kb on - strand at 2746.983 kb on + strand at 2746.983 kb on + strand at 2746.983 kb on + strand at 2746.983 kb on + strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2746.984 kb on - strand at 2747.004 kb on + strand at 2747.283 kb on + strand, within LRK53_RS12375 at 2747.283 kb on + strand, within LRK53_RS12375 at 2747.283 kb on + strand, within LRK53_RS12375 at 2747.283 kb on + strand, within LRK53_RS12375 at 2747.284 kb on - strand, within LRK53_RS12375 at 2747.284 kb on - strand, within LRK53_RS12375 at 2747.284 kb on - strand, within LRK53_RS12375 at 2747.284 kb on - strand, within LRK53_RS12375 at 2747.284 kb on - strand, within LRK53_RS12375 at 2747.284 kb on - strand, within LRK53_RS12375 at 2747.763 kb on + strand, within LRK53_RS12375 at 2747.763 kb on + strand, within LRK53_RS12375 at 2747.764 kb on - strand, within LRK53_RS12375 at 2747.764 kb on - strand, within LRK53_RS12375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,745,476 + LRK53_RS12365 0.13 +1.0 2,745,476 + LRK53_RS12365 0.13 +1.0 2,745,482 + LRK53_RS12365 0.14 +2.0 2,745,560 + LRK53_RS12365 0.22 -0.6 2,745,560 + LRK53_RS12365 0.22 -1.0 2,745,560 + LRK53_RS12365 0.22 -0.2 2,745,560 + LRK53_RS12365 0.22 -2.2 2,745,560 + LRK53_RS12365 0.22 -1.6 2,745,560 + LRK53_RS12365 0.22 -1.0 2,745,560 + LRK53_RS12365 0.22 +2.6 2,745,561 - LRK53_RS12365 0.23 -0.5 2,745,561 - LRK53_RS12365 0.23 +1.7 2,745,561 - LRK53_RS12365 0.23 -0.6 2,745,561 - LRK53_RS12365 0.23 -0.5 2,745,764 + LRK53_RS12365 0.46 +0.4 2,745,865 + LRK53_RS12365 0.57 -1.6 2,745,865 + LRK53_RS12365 0.57 -0.0 2,745,865 + LRK53_RS12365 0.57 -1.1 2,745,865 + LRK53_RS12365 0.57 -0.0 2,746,073 + LRK53_RS12365 0.80 +2.0 2,746,073 + LRK53_RS12365 0.80 -0.6 2,746,073 + LRK53_RS12365 0.80 +2.6 2,746,074 - LRK53_RS12365 0.81 -1.5 2,746,074 - LRK53_RS12365 0.81 -1.6 2,746,074 - LRK53_RS12365 0.81 -0.3 2,746,074 - LRK53_RS12365 0.81 +0.6 2,746,269 - -1.9 2,746,269 - -0.0 2,746,269 - -2.8 2,746,569 + LRK53_RS12370 0.49 -1.5 2,746,729 - LRK53_RS12370 0.86 +1.0 2,746,729 - LRK53_RS12370 0.86 -3.3 2,746,771 - +0.8 2,746,771 - -0.2 2,746,771 - -1.8 2,746,903 - -1.0 2,746,956 + -0.8 2,746,956 + +1.0 2,746,957 - -1.2 2,746,957 - +1.0 2,746,957 - +1.0 2,746,957 - +2.0 2,746,983 + -0.0 2,746,983 + -0.6 2,746,983 + +1.0 2,746,983 + +0.6 2,746,984 - -0.0 2,746,984 - -0.9 2,746,984 - -1.6 2,746,984 - -1.0 2,746,984 - +0.5 2,746,984 - -0.3 2,746,984 - +0.2 2,746,984 - -0.6 2,746,984 - +0.1 2,746,984 - -1.1 2,747,004 + -1.0 2,747,283 + LRK53_RS12375 0.27 +1.0 2,747,283 + LRK53_RS12375 0.27 +1.0 2,747,283 + LRK53_RS12375 0.27 +1.5 2,747,283 + LRK53_RS12375 0.27 -1.5 2,747,284 - LRK53_RS12375 0.27 +2.0 2,747,284 - LRK53_RS12375 0.27 -0.4 2,747,284 - LRK53_RS12375 0.27 +0.4 2,747,284 - LRK53_RS12375 0.27 -0.5 2,747,284 - LRK53_RS12375 0.27 -0.5 2,747,284 - LRK53_RS12375 0.27 +0.2 2,747,763 + LRK53_RS12375 0.59 -0.7 2,747,763 + LRK53_RS12375 0.59 -0.8 2,747,764 - LRK53_RS12375 0.59 -1.0 2,747,764 - LRK53_RS12375 0.59 -0.0
Or see this region's nucleotide sequence