Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS12265 and LRK53_RS12270 are separated by 3 nucleotides LRK53_RS12270 and LRK53_RS12275 are separated by 148 nucleotides LRK53_RS12275 and LRK53_RS12280 are separated by 153 nucleotides
LRK53_RS12265: LRK53_RS12265 - protein-L-isoaspartate(D-aspartate) O-methyltransferase, at 2,722,413 to 2,723,093
_RS12265
LRK53_RS12270: LRK53_RS12270 - 5'/3'-nucleotidase SurE, at 2,723,097 to 2,723,894
_RS12270
LRK53_RS12275: LRK53_RS12275 - Smr/MutS family protein, at 2,724,043 to 2,724,588
_RS12275
LRK53_RS12280: LRK53_RS12280 - tRNA pseudouridine(13) synthase TruD, at 2,724,742 to 2,725,752
_RS12280
Position (kb)
2724
2725 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2723.083 kb on + strand at 2723.083 kb on + strand at 2723.083 kb on + strand at 2723.083 kb on + strand at 2723.083 kb on + strand at 2723.083 kb on + strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.084 kb on - strand at 2723.494 kb on + strand, within LRK53_RS12270 at 2723.494 kb on + strand, within LRK53_RS12270 at 2723.494 kb on + strand, within LRK53_RS12270 at 2723.494 kb on + strand, within LRK53_RS12270 at 2723.494 kb on + strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.495 kb on - strand, within LRK53_RS12270 at 2723.577 kb on + strand, within LRK53_RS12270 at 2723.590 kb on + strand, within LRK53_RS12270 at 2723.590 kb on + strand, within LRK53_RS12270 at 2723.590 kb on + strand, within LRK53_RS12270 at 2723.590 kb on + strand, within LRK53_RS12270 at 2723.590 kb on + strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.591 kb on - strand, within LRK53_RS12270 at 2723.714 kb on - strand, within LRK53_RS12270 at 2723.999 kb on - strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.031 kb on + strand at 2724.032 kb on - strand at 2724.032 kb on - strand at 2724.032 kb on - strand at 2724.032 kb on - strand at 2724.032 kb on - strand at 2724.265 kb on + strand, within LRK53_RS12275 at 2724.265 kb on + strand, within LRK53_RS12275 at 2724.265 kb on + strand, within LRK53_RS12275 at 2724.265 kb on + strand, within LRK53_RS12275 at 2724.265 kb on + strand, within LRK53_RS12275 at 2724.266 kb on - strand, within LRK53_RS12275 at 2724.266 kb on - strand, within LRK53_RS12275 at 2724.586 kb on + strand at 2724.586 kb on + strand at 2724.586 kb on + strand at 2724.587 kb on - strand at 2724.587 kb on - strand at 2724.587 kb on - strand at 2724.587 kb on - strand at 2724.621 kb on + strand at 2724.621 kb on + strand at 2724.622 kb on - strand at 2724.622 kb on - strand at 2724.622 kb on - strand at 2724.622 kb on - strand at 2724.622 kb on - strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.743 kb on + strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.744 kb on - strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.782 kb on + strand at 2724.783 kb on - strand at 2724.783 kb on - strand at 2724.783 kb on - strand at 2724.783 kb on - strand at 2724.783 kb on - strand at 2724.783 kb on - strand at 2724.783 kb on - strand at 2725.283 kb on + strand, within LRK53_RS12280 at 2725.283 kb on + strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280 at 2725.284 kb on - strand, within LRK53_RS12280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,723,083 + -1.8 2,723,083 + -1.6 2,723,083 + -1.1 2,723,083 + -1.0 2,723,083 + -0.3 2,723,083 + -3.5 2,723,084 - -1.2 2,723,084 - -1.5 2,723,084 - -1.0 2,723,084 - -2.1 2,723,084 - -0.0 2,723,084 - -0.0 2,723,084 - -0.8 2,723,084 - -0.3 2,723,084 - -1.8 2,723,084 - -0.4 2,723,084 - -1.4 2,723,084 - -0.0 2,723,084 - -2.6 2,723,084 - -1.0 2,723,084 - +1.0 2,723,494 + LRK53_RS12270 0.50 -0.6 2,723,494 + LRK53_RS12270 0.50 -1.0 2,723,494 + LRK53_RS12270 0.50 -1.3 2,723,494 + LRK53_RS12270 0.50 -0.0 2,723,494 + LRK53_RS12270 0.50 -0.5 2,723,495 - LRK53_RS12270 0.50 -2.0 2,723,495 - LRK53_RS12270 0.50 -0.0 2,723,495 - LRK53_RS12270 0.50 -0.3 2,723,495 - LRK53_RS12270 0.50 -2.0 2,723,495 - LRK53_RS12270 0.50 -0.0 2,723,495 - LRK53_RS12270 0.50 -0.7 2,723,495 - LRK53_RS12270 0.50 +1.0 2,723,577 + LRK53_RS12270 0.60 -0.7 2,723,590 + LRK53_RS12270 0.62 -1.8 2,723,590 + LRK53_RS12270 0.62 -3.2 2,723,590 + LRK53_RS12270 0.62 -1.0 2,723,590 + LRK53_RS12270 0.62 -1.5 2,723,590 + LRK53_RS12270 0.62 -1.6 2,723,591 - LRK53_RS12270 0.62 -2.5 2,723,591 - LRK53_RS12270 0.62 +2.0 2,723,591 - LRK53_RS12270 0.62 -1.0 2,723,591 - LRK53_RS12270 0.62 -0.0 2,723,591 - LRK53_RS12270 0.62 -0.6 2,723,591 - LRK53_RS12270 0.62 -2.3 2,723,591 - LRK53_RS12270 0.62 -0.0 2,723,591 - LRK53_RS12270 0.62 +1.0 2,723,591 - LRK53_RS12270 0.62 +0.2 2,723,591 - LRK53_RS12270 0.62 -0.6 2,723,591 - LRK53_RS12270 0.62 -1.1 2,723,714 - LRK53_RS12270 0.77 -1.9 2,723,999 - -0.6 2,724,031 + +0.2 2,724,031 + -0.8 2,724,031 + +1.2 2,724,031 + +2.0 2,724,031 + -0.3 2,724,031 + -1.0 2,724,031 + -1.0 2,724,031 + -1.8 2,724,031 + -0.4 2,724,031 + -1.0 2,724,031 + -1.2 2,724,031 + -1.2 2,724,031 + -2.2 2,724,031 + -0.3 2,724,031 + -1.3 2,724,031 + -0.8 2,724,031 + +0.2 2,724,031 + -1.3 2,724,031 + -0.0 2,724,031 + -0.0 2,724,031 + -1.6 2,724,032 - -2.2 2,724,032 - -1.7 2,724,032 - -1.8 2,724,032 - -1.2 2,724,032 - -0.0 2,724,265 + LRK53_RS12275 0.41 -1.2 2,724,265 + LRK53_RS12275 0.41 -1.8 2,724,265 + LRK53_RS12275 0.41 -0.0 2,724,265 + LRK53_RS12275 0.41 +0.4 2,724,265 + LRK53_RS12275 0.41 -1.6 2,724,266 - LRK53_RS12275 0.41 -1.3 2,724,266 - LRK53_RS12275 0.41 +0.4 2,724,586 + -3.1 2,724,586 + +1.4 2,724,586 + -1.9 2,724,587 - +0.2 2,724,587 - -0.7 2,724,587 - -0.0 2,724,587 - +0.2 2,724,621 + +2.0 2,724,621 + -0.3 2,724,622 - -0.6 2,724,622 - -0.5 2,724,622 - -0.8 2,724,622 - -1.0 2,724,622 - +0.3 2,724,743 + -1.3 2,724,743 + -0.2 2,724,743 + -1.3 2,724,743 + -0.9 2,724,743 + -1.0 2,724,743 + -2.0 2,724,743 + -0.6 2,724,743 + -0.6 2,724,743 + -1.6 2,724,743 + -0.7 2,724,743 + +0.4 2,724,743 + -0.9 2,724,743 + -0.6 2,724,743 + -0.0 2,724,743 + -0.0 2,724,743 + +0.2 2,724,743 + -0.8 2,724,743 + -1.0 2,724,743 + +1.0 2,724,743 + +1.0 2,724,743 + -1.7 2,724,743 + -0.4 2,724,743 + +1.0 2,724,743 + -1.6 2,724,743 + +0.6 2,724,743 + -0.0 2,724,744 - +1.0 2,724,744 - +1.3 2,724,744 - -1.2 2,724,744 - -1.7 2,724,744 - +1.0 2,724,744 - -1.3 2,724,744 - -0.5 2,724,744 - +2.6 2,724,744 - +0.2 2,724,744 - +1.0 2,724,744 - +0.4 2,724,782 + -0.6 2,724,782 + -0.8 2,724,782 + -0.0 2,724,782 + -0.9 2,724,782 + -0.0 2,724,782 + +2.0 2,724,782 + -1.4 2,724,782 + +1.6 2,724,782 + -0.4 2,724,782 + -0.5 2,724,783 - -0.7 2,724,783 - -0.5 2,724,783 - -1.0 2,724,783 - +1.0 2,724,783 - -1.6 2,724,783 - -0.6 2,724,783 - -0.0 2,725,283 + LRK53_RS12280 0.54 -1.5 2,725,283 + LRK53_RS12280 0.54 +1.0 2,725,284 - LRK53_RS12280 0.54 -0.3 2,725,284 - LRK53_RS12280 0.54 -0.8 2,725,284 - LRK53_RS12280 0.54 -1.3 2,725,284 - LRK53_RS12280 0.54 -0.6 2,725,284 - LRK53_RS12280 0.54 -1.0
Or see this region's nucleotide sequence