Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11795 and LRK53_RS11800 overlap by 17 nucleotides LRK53_RS11800 and LRK53_RS11805 overlap by 4 nucleotides LRK53_RS11805 and LRK53_RS11810 overlap by 4 nucleotides LRK53_RS11810 and LRK53_RS11815 are separated by 237 nucleotides LRK53_RS11815 and LRK53_RS11820 are separated by 66 nucleotides
LRK53_RS11795: LRK53_RS11795 - cytochrome-c oxidase, cbb3-type subunit III, at 2,627,552 to 2,628,460
_RS11795
LRK53_RS11800: LRK53_RS11800 - hypothetical protein, at 2,628,444 to 2,628,680
_RS11800
LRK53_RS11805: LRK53_RS11805 - heavy metal translocating P-type ATPase, at 2,628,677 to 2,630,932
_RS11805
LRK53_RS11810: LRK53_RS11810 - cbb3-type cytochrome oxidase assembly protein CcoS, at 2,630,929 to 2,631,123
_RS11810
LRK53_RS11815: LRK53_RS11815 - hypothetical protein, at 2,631,361 to 2,631,552
_RS11815
LRK53_RS11820: LRK53_RS11820 - phospholipase D-like domain-containing protein, at 2,631,619 to 2,633,085
_RS11820
Position (kb)
2628
2629
2630
2631 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2627.772 kb on - strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.297 kb on + strand, within LRK53_RS11795 at 2628.298 kb on - strand, within LRK53_RS11795 at 2628.298 kb on - strand, within LRK53_RS11795 at 2628.416 kb on + strand at 2628.422 kb on + strand at 2628.423 kb on - strand at 2628.879 kb on + strand at 2629.052 kb on + strand, within LRK53_RS11805 at 2629.053 kb on - strand, within LRK53_RS11805 at 2629.053 kb on - strand, within LRK53_RS11805 at 2629.053 kb on - strand, within LRK53_RS11805 at 2629.053 kb on - strand, within LRK53_RS11805 at 2629.053 kb on - strand, within LRK53_RS11805 at 2629.068 kb on - strand, within LRK53_RS11805 at 2629.271 kb on - strand, within LRK53_RS11805 at 2629.271 kb on - strand, within LRK53_RS11805 at 2629.296 kb on - strand, within LRK53_RS11805 at 2629.296 kb on - strand, within LRK53_RS11805 at 2630.753 kb on + strand at 2630.753 kb on + strand at 2630.849 kb on + strand at 2630.850 kb on - strand at 2631.378 kb on + strand at 2631.379 kb on - strand at 2631.380 kb on + strand at 2631.380 kb on + strand at 2631.380 kb on + strand at 2631.380 kb on + strand at 2631.380 kb on + strand at 2631.380 kb on + strand at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.381 kb on - strand, within LRK53_RS11815 at 2631.422 kb on + strand, within LRK53_RS11815 at 2631.422 kb on + strand, within LRK53_RS11815 at 2631.423 kb on - strand, within LRK53_RS11815 at 2631.423 kb on - strand, within LRK53_RS11815 at 2631.506 kb on + strand, within LRK53_RS11815 at 2631.506 kb on + strand, within LRK53_RS11815 at 2631.507 kb on - strand, within LRK53_RS11815 at 2631.607 kb on + strand at 2631.607 kb on + strand at 2631.607 kb on + strand at 2631.608 kb on - strand at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.802 kb on + strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820 at 2631.803 kb on - strand, within LRK53_RS11820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,627,772 - LRK53_RS11795 0.24 +1.0 2,628,297 + LRK53_RS11795 0.82 -0.0 2,628,297 + LRK53_RS11795 0.82 +1.0 2,628,297 + LRK53_RS11795 0.82 +1.0 2,628,297 + LRK53_RS11795 0.82 +1.0 2,628,297 + LRK53_RS11795 0.82 -0.0 2,628,297 + LRK53_RS11795 0.82 +1.0 2,628,297 + LRK53_RS11795 0.82 -0.6 2,628,297 + LRK53_RS11795 0.82 -0.0 2,628,298 - LRK53_RS11795 0.82 +1.0 2,628,298 - LRK53_RS11795 0.82 -0.0 2,628,416 + +1.0 2,628,422 + -0.0 2,628,423 - -0.0 2,628,879 + -0.6 2,629,052 + LRK53_RS11805 0.17 -1.0 2,629,053 - LRK53_RS11805 0.17 -0.6 2,629,053 - LRK53_RS11805 0.17 -0.6 2,629,053 - LRK53_RS11805 0.17 -0.6 2,629,053 - LRK53_RS11805 0.17 +2.0 2,629,053 - LRK53_RS11805 0.17 -0.0 2,629,068 - LRK53_RS11805 0.17 +1.0 2,629,271 - LRK53_RS11805 0.26 +1.0 2,629,271 - LRK53_RS11805 0.26 -0.0 2,629,296 - LRK53_RS11805 0.27 +1.0 2,629,296 - LRK53_RS11805 0.27 +1.0 2,630,753 + +1.0 2,630,753 + +1.0 2,630,849 + -1.3 2,630,850 - -0.6 2,631,378 + -0.5 2,631,379 - -0.3 2,631,380 + -1.0 2,631,380 + +0.2 2,631,380 + -1.2 2,631,380 + -0.9 2,631,380 + +2.0 2,631,380 + -0.6 2,631,381 - LRK53_RS11815 0.10 -1.0 2,631,381 - LRK53_RS11815 0.10 -1.6 2,631,381 - LRK53_RS11815 0.10 -0.7 2,631,381 - LRK53_RS11815 0.10 -0.0 2,631,381 - LRK53_RS11815 0.10 +0.6 2,631,381 - LRK53_RS11815 0.10 -1.5 2,631,381 - LRK53_RS11815 0.10 +1.0 2,631,381 - LRK53_RS11815 0.10 -0.0 2,631,381 - LRK53_RS11815 0.10 -1.3 2,631,381 - LRK53_RS11815 0.10 +1.0 2,631,381 - LRK53_RS11815 0.10 -0.3 2,631,381 - LRK53_RS11815 0.10 -1.8 2,631,381 - LRK53_RS11815 0.10 -1.3 2,631,381 - LRK53_RS11815 0.10 +0.2 2,631,381 - LRK53_RS11815 0.10 -0.3 2,631,381 - LRK53_RS11815 0.10 -0.1 2,631,381 - LRK53_RS11815 0.10 -0.6 2,631,422 + LRK53_RS11815 0.32 -1.3 2,631,422 + LRK53_RS11815 0.32 -1.0 2,631,423 - LRK53_RS11815 0.32 -0.6 2,631,423 - LRK53_RS11815 0.32 +2.6 2,631,506 + LRK53_RS11815 0.76 -0.3 2,631,506 + LRK53_RS11815 0.76 -0.4 2,631,507 - LRK53_RS11815 0.76 -1.1 2,631,607 + -1.3 2,631,607 + -1.0 2,631,607 + -1.0 2,631,608 - -1.0 2,631,802 + LRK53_RS11820 0.12 -0.6 2,631,802 + LRK53_RS11820 0.12 -1.6 2,631,802 + LRK53_RS11820 0.12 +0.6 2,631,802 + LRK53_RS11820 0.12 -0.0 2,631,802 + LRK53_RS11820 0.12 +0.4 2,631,802 + LRK53_RS11820 0.12 +0.3 2,631,802 + LRK53_RS11820 0.12 -0.0 2,631,802 + LRK53_RS11820 0.12 -1.0 2,631,803 - LRK53_RS11820 0.13 +0.2 2,631,803 - LRK53_RS11820 0.13 -0.3 2,631,803 - LRK53_RS11820 0.13 -0.0 2,631,803 - LRK53_RS11820 0.13 -0.8 2,631,803 - LRK53_RS11820 0.13 -1.0 2,631,803 - LRK53_RS11820 0.13 -0.8 2,631,803 - LRK53_RS11820 0.13 +1.0 2,631,803 - LRK53_RS11820 0.13 +1.0
Or see this region's nucleotide sequence