Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11610 and LRK53_RS11615 are separated by 40 nucleotides LRK53_RS11615 and LRK53_RS11620 overlap by 2 nucleotides LRK53_RS11620 and LRK53_RS11625 are separated by 620 nucleotides
LRK53_RS11610: LRK53_RS11610 - low molecular weight phosphotyrosine protein phosphatase, at 2,587,408 to 2,587,842
_RS11610
LRK53_RS11615: LRK53_RS11615 - hypothetical protein, at 2,587,883 to 2,588,311
_RS11615
LRK53_RS11620: LRK53_RS11620 - hypothetical protein, at 2,588,310 to 2,588,447
_RS11620
LRK53_RS11625: LRK53_RS11625 - Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB, at 2,589,068 to 2,590,345
_RS11625
Position (kb)
2588
2589 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2587.365 kb on + strand at 2587.366 kb on - strand at 2587.421 kb on + strand at 2587.421 kb on + strand at 2587.421 kb on + strand at 2587.422 kb on - strand at 2587.422 kb on - strand at 2587.422 kb on - strand at 2587.443 kb on + strand at 2587.443 kb on + strand at 2587.443 kb on + strand at 2587.443 kb on + strand at 2587.444 kb on - strand at 2587.444 kb on - strand at 2587.444 kb on - strand at 2587.444 kb on - strand at 2587.444 kb on - strand at 2587.444 kb on - strand at 2587.468 kb on + strand, within LRK53_RS11610 at 2587.468 kb on + strand, within LRK53_RS11610 at 2587.584 kb on + strand, within LRK53_RS11610 at 2587.685 kb on + strand, within LRK53_RS11610 at 2587.686 kb on - strand, within LRK53_RS11610 at 2587.717 kb on + strand, within LRK53_RS11610 at 2587.718 kb on - strand, within LRK53_RS11610 at 2587.718 kb on - strand, within LRK53_RS11610 at 2587.744 kb on - strand, within LRK53_RS11610 at 2587.750 kb on - strand, within LRK53_RS11610 at 2587.781 kb on + strand, within LRK53_RS11610 at 2587.803 kb on - strand at 2587.822 kb on - strand at 2587.822 kb on - strand at 2587.822 kb on - strand at 2587.835 kb on - strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.901 kb on + strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.902 kb on - strand at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.940 kb on + strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2587.941 kb on - strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.204 kb on + strand, within LRK53_RS11615 at 2588.205 kb on - strand, within LRK53_RS11615 at 2588.205 kb on - strand, within LRK53_RS11615 at 2588.205 kb on - strand, within LRK53_RS11615 at 2588.300 kb on + strand at 2588.300 kb on + strand at 2588.300 kb on + strand at 2588.402 kb on + strand, within LRK53_RS11620 at 2588.402 kb on + strand, within LRK53_RS11620 at 2588.402 kb on + strand, within LRK53_RS11620 at 2588.403 kb on - strand, within LRK53_RS11620 at 2588.403 kb on - strand, within LRK53_RS11620 at 2588.403 kb on - strand, within LRK53_RS11620 at 2588.445 kb on + strand at 2588.445 kb on + strand at 2588.445 kb on + strand at 2588.445 kb on + strand at 2588.445 kb on + strand at 2588.445 kb on + strand at 2588.446 kb on - strand at 2588.446 kb on - strand at 2588.446 kb on - strand at 2588.539 kb on + strand at 2588.539 kb on + strand at 2588.540 kb on - strand at 2588.540 kb on - strand at 2588.540 kb on - strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.752 kb on + strand at 2588.753 kb on - strand at 2588.753 kb on - strand at 2588.803 kb on + strand at 2588.803 kb on + strand at 2588.803 kb on + strand at 2588.803 kb on + strand at 2588.804 kb on - strand at 2588.804 kb on - strand at 2588.967 kb on + strand at 2588.967 kb on + strand at 2588.967 kb on + strand at 2588.967 kb on + strand at 2588.968 kb on - strand at 2588.968 kb on - strand at 2588.968 kb on - strand at 2588.968 kb on - strand at 2588.968 kb on - strand at 2588.968 kb on - strand at 2589.067 kb on + strand at 2589.068 kb on - strand at 2589.113 kb on + strand at 2589.113 kb on + strand at 2589.113 kb on + strand at 2589.113 kb on + strand at 2589.114 kb on - strand at 2589.114 kb on - strand at 2589.114 kb on - strand at 2589.114 kb on - strand at 2589.114 kb on - strand at 2589.152 kb on + strand at 2589.152 kb on + strand at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.305 kb on + strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.306 kb on - strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.419 kb on + strand, within LRK53_RS11625 at 2589.420 kb on - strand, within LRK53_RS11625 at 2589.420 kb on - strand, within LRK53_RS11625 at 2589.420 kb on - strand, within LRK53_RS11625 at 2589.420 kb on - strand, within LRK53_RS11625 at 2589.420 kb on - strand, within LRK53_RS11625 at 2589.420 kb on - strand, within LRK53_RS11625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,587,365 + -0.2 2,587,366 - -1.3 2,587,421 + -0.6 2,587,421 + +0.8 2,587,421 + -3.7 2,587,422 - -1.8 2,587,422 - +0.3 2,587,422 - -1.0 2,587,443 + -0.5 2,587,443 + -2.2 2,587,443 + -1.2 2,587,443 + +2.0 2,587,444 - +1.0 2,587,444 - -0.0 2,587,444 - +0.7 2,587,444 - +1.0 2,587,444 - -1.0 2,587,444 - -1.0 2,587,468 + LRK53_RS11610 0.14 -0.3 2,587,468 + LRK53_RS11610 0.14 -1.5 2,587,584 + LRK53_RS11610 0.40 -0.0 2,587,685 + LRK53_RS11610 0.64 -1.6 2,587,686 - LRK53_RS11610 0.64 -1.6 2,587,717 + LRK53_RS11610 0.71 -0.5 2,587,718 - LRK53_RS11610 0.71 -0.8 2,587,718 - LRK53_RS11610 0.71 -0.0 2,587,744 - LRK53_RS11610 0.77 +0.5 2,587,750 - LRK53_RS11610 0.79 -1.3 2,587,781 + LRK53_RS11610 0.86 -1.8 2,587,803 - -1.8 2,587,822 - -0.6 2,587,822 - -0.0 2,587,822 - -0.0 2,587,835 - -2.2 2,587,901 + -1.6 2,587,901 + +0.6 2,587,901 + -0.3 2,587,901 + -0.0 2,587,901 + -1.3 2,587,901 + -1.3 2,587,901 + -0.2 2,587,901 + -2.5 2,587,901 + -0.6 2,587,901 + -0.7 2,587,901 + -0.3 2,587,901 + +1.0 2,587,901 + -1.8 2,587,901 + +0.5 2,587,901 + +0.2 2,587,901 + +1.0 2,587,901 + +0.4 2,587,901 + -1.0 2,587,901 + -1.3 2,587,902 - -0.0 2,587,902 - +1.0 2,587,902 - +1.0 2,587,902 - -1.8 2,587,902 - -0.9 2,587,902 - -0.0 2,587,902 - -1.8 2,587,902 - -0.0 2,587,902 - -0.9 2,587,902 - +1.0 2,587,902 - -0.6 2,587,940 + LRK53_RS11615 0.13 -0.0 2,587,940 + LRK53_RS11615 0.13 +2.0 2,587,940 + LRK53_RS11615 0.13 +0.3 2,587,940 + LRK53_RS11615 0.13 -0.2 2,587,940 + LRK53_RS11615 0.13 -0.0 2,587,940 + LRK53_RS11615 0.13 -2.0 2,587,940 + LRK53_RS11615 0.13 -2.3 2,587,940 + LRK53_RS11615 0.13 -0.6 2,587,940 + LRK53_RS11615 0.13 -1.6 2,587,940 + LRK53_RS11615 0.13 +0.3 2,587,940 + LRK53_RS11615 0.13 -2.5 2,587,940 + LRK53_RS11615 0.13 -0.4 2,587,940 + LRK53_RS11615 0.13 -0.7 2,587,940 + LRK53_RS11615 0.13 -0.8 2,587,940 + LRK53_RS11615 0.13 +0.3 2,587,940 + LRK53_RS11615 0.13 -1.3 2,587,940 + LRK53_RS11615 0.13 -1.3 2,587,940 + LRK53_RS11615 0.13 +0.4 2,587,940 + LRK53_RS11615 0.13 +1.0 2,587,941 - LRK53_RS11615 0.14 -0.5 2,587,941 - LRK53_RS11615 0.14 -0.6 2,587,941 - LRK53_RS11615 0.14 -0.6 2,587,941 - LRK53_RS11615 0.14 -0.0 2,587,941 - LRK53_RS11615 0.14 +0.4 2,587,941 - LRK53_RS11615 0.14 +0.2 2,587,941 - LRK53_RS11615 0.14 -0.6 2,587,941 - LRK53_RS11615 0.14 -2.5 2,587,941 - LRK53_RS11615 0.14 -0.7 2,588,204 + LRK53_RS11615 0.75 -1.2 2,588,204 + LRK53_RS11615 0.75 -0.6 2,588,204 + LRK53_RS11615 0.75 -1.7 2,588,204 + LRK53_RS11615 0.75 -0.2 2,588,204 + LRK53_RS11615 0.75 -0.7 2,588,204 + LRK53_RS11615 0.75 -1.8 2,588,204 + LRK53_RS11615 0.75 -0.5 2,588,205 - LRK53_RS11615 0.75 -0.4 2,588,205 - LRK53_RS11615 0.75 -1.0 2,588,205 - LRK53_RS11615 0.75 +1.0 2,588,300 + +0.2 2,588,300 + -0.0 2,588,300 + -1.0 2,588,402 + LRK53_RS11620 0.67 -0.4 2,588,402 + LRK53_RS11620 0.67 +1.6 2,588,402 + LRK53_RS11620 0.67 -0.0 2,588,403 - LRK53_RS11620 0.67 +1.0 2,588,403 - LRK53_RS11620 0.67 -0.6 2,588,403 - LRK53_RS11620 0.67 -0.2 2,588,445 + -0.2 2,588,445 + -1.3 2,588,445 + -0.0 2,588,445 + -0.0 2,588,445 + +0.2 2,588,445 + +0.4 2,588,446 - -2.0 2,588,446 - -0.6 2,588,446 - -0.8 2,588,539 + +0.3 2,588,539 + -0.5 2,588,540 - -1.2 2,588,540 - +0.7 2,588,540 - +1.0 2,588,752 + -1.0 2,588,752 + -0.0 2,588,752 + -0.6 2,588,752 + +1.0 2,588,752 + -1.2 2,588,752 + -1.9 2,588,752 + -1.3 2,588,752 + -0.0 2,588,753 - -0.2 2,588,753 - +1.0 2,588,803 + -1.3 2,588,803 + -0.2 2,588,803 + -0.6 2,588,803 + +0.8 2,588,804 - +1.0 2,588,804 - +0.5 2,588,967 + -2.0 2,588,967 + -0.3 2,588,967 + -0.9 2,588,967 + +1.0 2,588,968 - +0.7 2,588,968 - -0.2 2,588,968 - +1.4 2,588,968 - +0.4 2,588,968 - -0.6 2,588,968 - -0.0 2,589,067 + -0.0 2,589,068 - +0.2 2,589,113 + +1.0 2,589,113 + +0.2 2,589,113 + -0.5 2,589,113 + -0.7 2,589,114 - +2.0 2,589,114 - -0.2 2,589,114 - -0.7 2,589,114 - -0.2 2,589,114 - -0.6 2,589,152 + +1.0 2,589,152 + +1.0 2,589,305 + LRK53_RS11625 0.19 -1.8 2,589,305 + LRK53_RS11625 0.19 -0.7 2,589,305 + LRK53_RS11625 0.19 -1.6 2,589,305 + LRK53_RS11625 0.19 -1.0 2,589,305 + LRK53_RS11625 0.19 +0.2 2,589,305 + LRK53_RS11625 0.19 +0.0 2,589,305 + LRK53_RS11625 0.19 -0.6 2,589,305 + LRK53_RS11625 0.19 +1.0 2,589,305 + LRK53_RS11625 0.19 -1.1 2,589,305 + LRK53_RS11625 0.19 -1.0 2,589,305 + LRK53_RS11625 0.19 -0.0 2,589,305 + LRK53_RS11625 0.19 +2.6 2,589,305 + LRK53_RS11625 0.19 -0.7 2,589,305 + LRK53_RS11625 0.19 +2.0 2,589,306 - LRK53_RS11625 0.19 -2.5 2,589,306 - LRK53_RS11625 0.19 -0.6 2,589,306 - LRK53_RS11625 0.19 -0.8 2,589,306 - LRK53_RS11625 0.19 -0.5 2,589,306 - LRK53_RS11625 0.19 +0.3 2,589,306 - LRK53_RS11625 0.19 -0.0 2,589,306 - LRK53_RS11625 0.19 +1.6 2,589,306 - LRK53_RS11625 0.19 -1.0 2,589,306 - LRK53_RS11625 0.19 -1.6 2,589,306 - LRK53_RS11625 0.19 -0.0 2,589,306 - LRK53_RS11625 0.19 +1.0 2,589,306 - LRK53_RS11625 0.19 -0.4 2,589,306 - LRK53_RS11625 0.19 -1.3 2,589,419 + LRK53_RS11625 0.27 -1.0 2,589,419 + LRK53_RS11625 0.27 -0.4 2,589,419 + LRK53_RS11625 0.27 -1.3 2,589,419 + LRK53_RS11625 0.27 -1.0 2,589,419 + LRK53_RS11625 0.27 -0.5 2,589,419 + LRK53_RS11625 0.27 -0.2 2,589,419 + LRK53_RS11625 0.27 +1.0 2,589,419 + LRK53_RS11625 0.27 +1.0 2,589,419 + LRK53_RS11625 0.27 -1.0 2,589,419 + LRK53_RS11625 0.27 -0.0 2,589,419 + LRK53_RS11625 0.27 -1.0 2,589,420 - LRK53_RS11625 0.28 +0.8 2,589,420 - LRK53_RS11625 0.28 -0.0 2,589,420 - LRK53_RS11625 0.28 -0.6 2,589,420 - LRK53_RS11625 0.28 -0.9 2,589,420 - LRK53_RS11625 0.28 -1.3 2,589,420 - LRK53_RS11625 0.28 +0.4
Or see this region's nucleotide sequence