Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11415 and LRK53_RS11420 are separated by 74 nucleotides LRK53_RS11420 and LRK53_RS11425 are separated by 17 nucleotides LRK53_RS11425 and LRK53_RS11430 overlap by 8 nucleotides LRK53_RS11430 and LRK53_RS11435 are separated by 204 nucleotides
LRK53_RS11415: LRK53_RS11415 - ferric iron uptake transcriptional regulator, at 2,546,184 to 2,546,606
_RS11415
LRK53_RS11420: LRK53_RS11420 - outer membrane protein assembly factor BamE, at 2,546,681 to 2,547,151
_RS11420
LRK53_RS11425: LRK53_RS11425 - RnfH family protein, at 2,547,169 to 2,547,447
_RS11425
LRK53_RS11430: LRK53_RS11430 - type II toxin-antitoxin system RatA family toxin, at 2,547,440 to 2,547,871
_RS11430
LRK53_RS11435: LRK53_RS11435 - SsrA-binding protein SmpB, at 2,548,076 to 2,548,567
_RS11435
Position (kb)
2547
2548 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.644 kb on + strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.645 kb on - strand at 2546.863 kb on + strand, within LRK53_RS11420 at 2546.863 kb on + strand, within LRK53_RS11420 at 2546.891 kb on + strand, within LRK53_RS11420 at 2546.891 kb on + strand, within LRK53_RS11420 at 2546.892 kb on - strand, within LRK53_RS11420 at 2546.895 kb on + strand, within LRK53_RS11420 at 2546.962 kb on + strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.963 kb on - strand, within LRK53_RS11420 at 2546.974 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.032 kb on + strand, within LRK53_RS11420 at 2547.033 kb on - strand, within LRK53_RS11420 at 2547.033 kb on - strand, within LRK53_RS11420 at 2547.033 kb on - strand, within LRK53_RS11420 at 2547.033 kb on - strand, within LRK53_RS11420 at 2547.033 kb on - strand, within LRK53_RS11420 at 2547.221 kb on + strand, within LRK53_RS11425 at 2547.221 kb on + strand, within LRK53_RS11425 at 2547.221 kb on + strand, within LRK53_RS11425 at 2547.222 kb on - strand, within LRK53_RS11425 at 2547.222 kb on - strand, within LRK53_RS11425 at 2547.222 kb on - strand, within LRK53_RS11425 at 2547.413 kb on + strand, within LRK53_RS11425 at 2547.414 kb on - strand, within LRK53_RS11425 at 2547.946 kb on + strand at 2547.946 kb on + strand at 2547.946 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,546,644 + +1.7 2,546,644 + -1.4 2,546,644 + -2.2 2,546,644 + -1.3 2,546,644 + -0.2 2,546,644 + -0.4 2,546,644 + -0.0 2,546,644 + -0.7 2,546,644 + +0.4 2,546,644 + -0.1 2,546,644 + -0.6 2,546,644 + -1.6 2,546,644 + -0.0 2,546,644 + -0.4 2,546,644 + +1.0 2,546,644 + -0.6 2,546,644 + -2.4 2,546,644 + -1.4 2,546,644 + -0.1 2,546,644 + -0.4 2,546,644 + +0.1 2,546,644 + -0.3 2,546,644 + -1.4 2,546,644 + +1.0 2,546,644 + -1.4 2,546,645 - +1.0 2,546,645 - -3.2 2,546,645 - -0.8 2,546,645 - -0.6 2,546,645 - -1.6 2,546,645 - -0.6 2,546,645 - -0.0 2,546,645 - +0.2 2,546,645 - -0.3 2,546,645 - +0.4 2,546,645 - -1.6 2,546,645 - -0.6 2,546,863 + LRK53_RS11420 0.39 +1.0 2,546,863 + LRK53_RS11420 0.39 -0.0 2,546,891 + LRK53_RS11420 0.45 +1.0 2,546,891 + LRK53_RS11420 0.45 +1.0 2,546,892 - LRK53_RS11420 0.45 +1.0 2,546,895 + LRK53_RS11420 0.45 +1.0 2,546,962 + LRK53_RS11420 0.60 -0.0 2,546,963 - LRK53_RS11420 0.60 -0.0 2,546,963 - LRK53_RS11420 0.60 -0.6 2,546,963 - LRK53_RS11420 0.60 -0.6 2,546,963 - LRK53_RS11420 0.60 +1.6 2,546,963 - LRK53_RS11420 0.60 +0.6 2,546,963 - LRK53_RS11420 0.60 -0.8 2,546,963 - LRK53_RS11420 0.60 -0.0 2,546,963 - LRK53_RS11420 0.60 -0.0 2,546,974 + LRK53_RS11420 0.62 +1.0 2,547,032 + LRK53_RS11420 0.75 -1.8 2,547,032 + LRK53_RS11420 0.75 -1.3 2,547,032 + LRK53_RS11420 0.75 -0.8 2,547,032 + LRK53_RS11420 0.75 -1.0 2,547,032 + LRK53_RS11420 0.75 -0.0 2,547,032 + LRK53_RS11420 0.75 +0.5 2,547,032 + LRK53_RS11420 0.75 -0.6 2,547,032 + LRK53_RS11420 0.75 +0.4 2,547,033 - LRK53_RS11420 0.75 -1.2 2,547,033 - LRK53_RS11420 0.75 -0.6 2,547,033 - LRK53_RS11420 0.75 +1.0 2,547,033 - LRK53_RS11420 0.75 -0.3 2,547,033 - LRK53_RS11420 0.75 -2.4 2,547,221 + LRK53_RS11425 0.19 -0.4 2,547,221 + LRK53_RS11425 0.19 -1.5 2,547,221 + LRK53_RS11425 0.19 -2.0 2,547,222 - LRK53_RS11425 0.19 +1.6 2,547,222 - LRK53_RS11425 0.19 -1.5 2,547,222 - LRK53_RS11425 0.19 -0.9 2,547,413 + LRK53_RS11425 0.87 -0.6 2,547,414 - LRK53_RS11425 0.88 -0.0 2,547,946 + -2.4 2,547,946 + -0.5 2,547,946 + -0.0
Or see this region's nucleotide sequence