Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11325 and LRK53_RS11330 are separated by 176 nucleotides LRK53_RS11330 and LRK53_RS11335 are separated by 111 nucleotides LRK53_RS11335 and LRK53_RS11340 are separated by 88 nucleotides
LRK53_RS11325: LRK53_RS11325 - 30S ribosomal protein S1, at 2,523,692 to 2,525,365
_RS11325
LRK53_RS11330: LRK53_RS11330 - (d)CMP kinase, at 2,525,542 to 2,526,219
_RS11330
LRK53_RS11335: LRK53_RS11335 - acyl-CoA-binding protein, at 2,526,331 to 2,526,588
_RS11335
LRK53_RS11340: LRK53_RS11340 - NADPH-dependent 2,4-dienoyl-CoA reductase, at 2,526,677 to 2,528,695
_RS11340
Position (kb)
2525
2526
2527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.564 kb on - strand at 2525.602 kb on - strand at 2525.704 kb on - strand, within LRK53_RS11330 at 2525.704 kb on - strand, within LRK53_RS11330 at 2525.733 kb on + strand, within LRK53_RS11330 at 2525.733 kb on + strand, within LRK53_RS11330 at 2525.733 kb on + strand, within LRK53_RS11330 at 2525.733 kb on + strand, within LRK53_RS11330 at 2525.733 kb on + strand, within LRK53_RS11330 at 2525.733 kb on + strand, within LRK53_RS11330 at 2525.734 kb on - strand, within LRK53_RS11330 at 2525.735 kb on + strand, within LRK53_RS11330 at 2525.736 kb on - strand, within LRK53_RS11330 at 2526.081 kb on - strand, within LRK53_RS11330 at 2526.095 kb on + strand, within LRK53_RS11330 at 2526.269 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.285 kb on + strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.286 kb on - strand at 2526.289 kb on + strand at 2526.289 kb on + strand at 2526.290 kb on - strand at 2526.290 kb on - strand at 2526.290 kb on - strand at 2526.330 kb on + strand at 2526.330 kb on + strand at 2526.330 kb on + strand at 2526.330 kb on + strand at 2526.330 kb on + strand at 2526.330 kb on + strand at 2526.331 kb on - strand at 2526.331 kb on - strand at 2526.331 kb on - strand at 2526.331 kb on - strand at 2526.331 kb on - strand at 2526.421 kb on + strand, within LRK53_RS11335 at 2526.421 kb on + strand, within LRK53_RS11335 at 2526.430 kb on + strand, within LRK53_RS11335 at 2526.430 kb on + strand, within LRK53_RS11335 at 2526.431 kb on - strand, within LRK53_RS11335 at 2526.431 kb on - strand, within LRK53_RS11335 at 2526.431 kb on - strand, within LRK53_RS11335 at 2526.556 kb on + strand, within LRK53_RS11335 at 2526.556 kb on + strand, within LRK53_RS11335 at 2526.556 kb on + strand, within LRK53_RS11335 at 2526.556 kb on + strand, within LRK53_RS11335 at 2526.556 kb on + strand, within LRK53_RS11335 at 2526.557 kb on - strand, within LRK53_RS11335 at 2526.557 kb on - strand, within LRK53_RS11335 at 2526.571 kb on + strand at 2526.571 kb on + strand at 2526.571 kb on + strand at 2526.571 kb on + strand at 2526.572 kb on - strand at 2526.572 kb on - strand at 2526.649 kb on + strand at 2526.683 kb on + strand at 2526.683 kb on + strand at 2526.683 kb on + strand at 2526.785 kb on + strand at 2526.785 kb on + strand at 2526.785 kb on + strand at 2526.786 kb on - strand at 2526.790 kb on + strand at 2526.790 kb on + strand at 2526.790 kb on + strand at 2526.790 kb on + strand at 2526.790 kb on + strand at 2526.790 kb on + strand at 2526.791 kb on - strand at 2526.791 kb on - strand at 2526.791 kb on - strand at 2526.803 kb on + strand at 2526.803 kb on + strand at 2526.803 kb on + strand at 2526.803 kb on + strand at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.967 kb on + strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.968 kb on - strand, within LRK53_RS11340 at 2526.999 kb on - strand, within LRK53_RS11340 at 2527.005 kb on - strand, within LRK53_RS11340 at 2527.005 kb on - strand, within LRK53_RS11340 at 2527.175 kb on + strand, within LRK53_RS11340 at 2527.175 kb on + strand, within LRK53_RS11340 at 2527.175 kb on + strand, within LRK53_RS11340 at 2527.175 kb on + strand, within LRK53_RS11340 at 2527.175 kb on + strand, within LRK53_RS11340 at 2527.176 kb on - strand, within LRK53_RS11340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,525,564 - -1.6 2,525,564 - +1.0 2,525,564 - -0.3 2,525,564 - -1.2 2,525,564 - -0.5 2,525,564 - -0.0 2,525,564 - -0.4 2,525,564 - +0.5 2,525,564 - -0.0 2,525,564 - +2.0 2,525,602 - -0.0 2,525,704 - LRK53_RS11330 0.24 -0.6 2,525,704 - LRK53_RS11330 0.24 -0.0 2,525,733 + LRK53_RS11330 0.28 +1.0 2,525,733 + LRK53_RS11330 0.28 -0.0 2,525,733 + LRK53_RS11330 0.28 -0.6 2,525,733 + LRK53_RS11330 0.28 -0.6 2,525,733 + LRK53_RS11330 0.28 +1.0 2,525,733 + LRK53_RS11330 0.28 +1.0 2,525,734 - LRK53_RS11330 0.28 -0.6 2,525,735 + LRK53_RS11330 0.28 -2.0 2,525,736 - LRK53_RS11330 0.29 -1.6 2,526,081 - LRK53_RS11330 0.79 -0.0 2,526,095 + LRK53_RS11330 0.82 +1.0 2,526,269 + -1.0 2,526,285 + -1.6 2,526,285 + -1.5 2,526,285 + +0.6 2,526,285 + -0.0 2,526,285 + -1.8 2,526,285 + -0.0 2,526,285 + +1.0 2,526,285 + -1.4 2,526,285 + +0.8 2,526,285 + -1.2 2,526,285 + +0.2 2,526,285 + +0.4 2,526,285 + -1.2 2,526,285 + -1.6 2,526,285 + -1.0 2,526,285 + +0.6 2,526,285 + -0.6 2,526,286 - -3.0 2,526,286 - -1.5 2,526,286 - +0.4 2,526,286 - -1.1 2,526,286 - -0.2 2,526,286 - -1.0 2,526,286 - -0.6 2,526,286 - +2.0 2,526,286 - -0.6 2,526,286 - +1.0 2,526,286 - -0.0 2,526,286 - -0.6 2,526,286 - -1.8 2,526,286 - -0.6 2,526,286 - -1.0 2,526,286 - -0.6 2,526,289 + -0.9 2,526,289 + +2.6 2,526,290 - +2.0 2,526,290 - -1.4 2,526,290 - -1.0 2,526,330 + +1.0 2,526,330 + -0.6 2,526,330 + -0.6 2,526,330 + -0.8 2,526,330 + -1.4 2,526,330 + -1.2 2,526,331 - -0.0 2,526,331 - -0.0 2,526,331 - -1.4 2,526,331 - -0.7 2,526,331 - -0.8 2,526,421 + LRK53_RS11335 0.35 -0.5 2,526,421 + LRK53_RS11335 0.35 -1.7 2,526,430 + LRK53_RS11335 0.38 +0.1 2,526,430 + LRK53_RS11335 0.38 +0.4 2,526,431 - LRK53_RS11335 0.39 -0.3 2,526,431 - LRK53_RS11335 0.39 -0.3 2,526,431 - LRK53_RS11335 0.39 -2.6 2,526,556 + LRK53_RS11335 0.87 -2.0 2,526,556 + LRK53_RS11335 0.87 -1.7 2,526,556 + LRK53_RS11335 0.87 -0.6 2,526,556 + LRK53_RS11335 0.87 +1.0 2,526,556 + LRK53_RS11335 0.87 -1.4 2,526,557 - LRK53_RS11335 0.88 +0.4 2,526,557 - LRK53_RS11335 0.88 -0.9 2,526,571 + -1.3 2,526,571 + -0.0 2,526,571 + -0.6 2,526,571 + -1.6 2,526,572 - -0.0 2,526,572 - +1.0 2,526,649 + -1.8 2,526,683 + -0.4 2,526,683 + -1.0 2,526,683 + -0.0 2,526,785 + -2.9 2,526,785 + +1.0 2,526,785 + -1.4 2,526,786 - +1.0 2,526,790 + -0.3 2,526,790 + +1.0 2,526,790 + -0.0 2,526,790 + -0.6 2,526,790 + -1.4 2,526,790 + -0.6 2,526,791 - -0.3 2,526,791 - -1.5 2,526,791 - -0.6 2,526,803 + -0.0 2,526,803 + +1.6 2,526,803 + -1.6 2,526,803 + +0.4 2,526,967 + LRK53_RS11340 0.14 -0.6 2,526,967 + LRK53_RS11340 0.14 +0.2 2,526,967 + LRK53_RS11340 0.14 -1.6 2,526,967 + LRK53_RS11340 0.14 -0.5 2,526,967 + LRK53_RS11340 0.14 -0.5 2,526,967 + LRK53_RS11340 0.14 +1.0 2,526,967 + LRK53_RS11340 0.14 -0.9 2,526,967 + LRK53_RS11340 0.14 -0.0 2,526,968 - LRK53_RS11340 0.14 -0.1 2,526,968 - LRK53_RS11340 0.14 +0.3 2,526,968 - LRK53_RS11340 0.14 -0.0 2,526,968 - LRK53_RS11340 0.14 -0.6 2,526,968 - LRK53_RS11340 0.14 -1.0 2,526,968 - LRK53_RS11340 0.14 -1.4 2,526,968 - LRK53_RS11340 0.14 -1.0 2,526,968 - LRK53_RS11340 0.14 +0.4 2,526,999 - LRK53_RS11340 0.16 -2.2 2,527,005 - LRK53_RS11340 0.16 -1.2 2,527,005 - LRK53_RS11340 0.16 -1.0 2,527,175 + LRK53_RS11340 0.25 +1.0 2,527,175 + LRK53_RS11340 0.25 +1.0 2,527,175 + LRK53_RS11340 0.25 -1.4 2,527,175 + LRK53_RS11340 0.25 -1.0 2,527,175 + LRK53_RS11340 0.25 -0.5 2,527,176 - LRK53_RS11340 0.25 +0.6
Or see this region's nucleotide sequence