Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11145 and LRK53_RS11150 are separated by 4 nucleotides LRK53_RS11150 and LRK53_RS11155 are separated by 79 nucleotides LRK53_RS11155 and LRK53_RS11160 are separated by 165 nucleotides
LRK53_RS11145: LRK53_RS11145 - VOC family protein, at 2,487,521 to 2,488,078
_RS11145
LRK53_RS11150: LRK53_RS11150 - tRNA adenosine(34) deaminase TadA, at 2,488,083 to 2,488,571
_RS11150
LRK53_RS11155: LRK53_RS11155 - glutamine-hydrolyzing GMP synthase, at 2,488,651 to 2,490,243
_RS11155
LRK53_RS11160: LRK53_RS11160 - IMP dehydrogenase, at 2,490,409 to 2,491,866
_RS11160
Position (kb)
2488
2489
2490
2491 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.020 kb on + strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.021 kb on - strand, within LRK53_RS11145 at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.593 kb on + strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.594 kb on - strand at 2488.635 kb on + strand at 2488.636 kb on - strand at 2488.636 kb on - strand at 2488.636 kb on - strand at 2488.636 kb on - strand at 2488.636 kb on - strand at 2488.653 kb on - strand at 2488.653 kb on - strand at 2488.695 kb on - strand at 2488.760 kb on + strand at 2488.760 kb on + strand at 2488.761 kb on - strand at 2488.761 kb on - strand at 2488.832 kb on + strand, within LRK53_RS11155 at 2488.833 kb on - strand, within LRK53_RS11155 at 2488.907 kb on + strand, within LRK53_RS11155 at 2488.907 kb on + strand, within LRK53_RS11155 at 2488.907 kb on + strand, within LRK53_RS11155 at 2488.907 kb on + strand, within LRK53_RS11155 at 2488.907 kb on + strand, within LRK53_RS11155 at 2488.908 kb on - strand, within LRK53_RS11155 at 2488.908 kb on - strand, within LRK53_RS11155 at 2488.970 kb on + strand, within LRK53_RS11155 at 2488.970 kb on + strand, within LRK53_RS11155 at 2488.970 kb on + strand, within LRK53_RS11155 at 2488.970 kb on + strand, within LRK53_RS11155 at 2488.971 kb on - strand, within LRK53_RS11155 at 2488.971 kb on - strand, within LRK53_RS11155 at 2489.033 kb on + strand, within LRK53_RS11155 at 2489.034 kb on - strand, within LRK53_RS11155 at 2489.216 kb on + strand, within LRK53_RS11155 at 2489.360 kb on + strand, within LRK53_RS11155 at 2489.360 kb on + strand, within LRK53_RS11155 at 2489.360 kb on + strand, within LRK53_RS11155 at 2489.361 kb on - strand, within LRK53_RS11155 at 2489.361 kb on - strand, within LRK53_RS11155 at 2489.697 kb on + strand, within LRK53_RS11155 at 2489.697 kb on + strand, within LRK53_RS11155 at 2489.698 kb on - strand, within LRK53_RS11155 at 2489.698 kb on - strand, within LRK53_RS11155 at 2489.698 kb on - strand, within LRK53_RS11155 at 2489.729 kb on + strand, within LRK53_RS11155 at 2489.730 kb on - strand, within LRK53_RS11155 at 2489.837 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.839 kb on + strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.840 kb on - strand, within LRK53_RS11155 at 2489.994 kb on + strand, within LRK53_RS11155 at 2489.994 kb on + strand, within LRK53_RS11155 at 2489.994 kb on + strand, within LRK53_RS11155 at 2489.994 kb on + strand, within LRK53_RS11155 at 2489.995 kb on - strand, within LRK53_RS11155 at 2489.995 kb on - strand, within LRK53_RS11155 at 2490.149 kb on + strand at 2490.149 kb on + strand at 2490.149 kb on + strand at 2490.150 kb on - strand at 2490.150 kb on - strand at 2490.191 kb on + strand at 2490.191 kb on + strand at 2490.191 kb on + strand at 2490.191 kb on + strand at 2490.191 kb on + strand at 2490.192 kb on - strand at 2490.192 kb on - strand at 2490.192 kb on - strand at 2490.192 kb on - strand at 2490.192 kb on - strand at 2490.192 kb on - strand at 2490.275 kb on + strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.276 kb on - strand at 2490.363 kb on + strand at 2490.363 kb on + strand at 2490.363 kb on + strand at 2490.363 kb on + strand at 2490.410 kb on + strand at 2490.410 kb on + strand at 2490.410 kb on + strand at 2490.410 kb on + strand at 2490.411 kb on - strand at 2490.411 kb on - strand at 2490.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,488,020 + LRK53_RS11145 0.89 -1.5 2,488,020 + LRK53_RS11145 0.89 -0.0 2,488,020 + LRK53_RS11145 0.89 -0.1 2,488,020 + LRK53_RS11145 0.89 -1.8 2,488,020 + LRK53_RS11145 0.89 -0.4 2,488,020 + LRK53_RS11145 0.89 -0.4 2,488,020 + LRK53_RS11145 0.89 -0.7 2,488,020 + LRK53_RS11145 0.89 +1.0 2,488,020 + LRK53_RS11145 0.89 +1.0 2,488,020 + LRK53_RS11145 0.89 +1.0 2,488,020 + LRK53_RS11145 0.89 -0.0 2,488,020 + LRK53_RS11145 0.89 -0.6 2,488,020 + LRK53_RS11145 0.89 +0.2 2,488,020 + LRK53_RS11145 0.89 +1.0 2,488,020 + LRK53_RS11145 0.89 -0.6 2,488,020 + LRK53_RS11145 0.89 -1.8 2,488,021 - LRK53_RS11145 0.90 +1.0 2,488,021 - LRK53_RS11145 0.90 -0.4 2,488,021 - LRK53_RS11145 0.90 -1.6 2,488,021 - LRK53_RS11145 0.90 +1.0 2,488,021 - LRK53_RS11145 0.90 +0.2 2,488,021 - LRK53_RS11145 0.90 +1.0 2,488,021 - LRK53_RS11145 0.90 -1.3 2,488,021 - LRK53_RS11145 0.90 -1.5 2,488,021 - LRK53_RS11145 0.90 -2.2 2,488,021 - LRK53_RS11145 0.90 -1.0 2,488,021 - LRK53_RS11145 0.90 -1.0 2,488,021 - LRK53_RS11145 0.90 -0.4 2,488,021 - LRK53_RS11145 0.90 -0.9 2,488,021 - LRK53_RS11145 0.90 +1.0 2,488,593 + -1.4 2,488,593 + -0.6 2,488,593 + -2.0 2,488,593 + -0.0 2,488,593 + -1.0 2,488,593 + -1.0 2,488,593 + -1.0 2,488,593 + -1.1 2,488,593 + -0.9 2,488,593 + -0.2 2,488,593 + -1.4 2,488,593 + -0.6 2,488,593 + -0.8 2,488,593 + +2.5 2,488,593 + -0.4 2,488,593 + -0.6 2,488,593 + -2.2 2,488,593 + -1.0 2,488,593 + -1.1 2,488,594 - -0.7 2,488,594 - -0.6 2,488,594 - -0.8 2,488,594 - +0.4 2,488,594 - +0.5 2,488,594 - +1.0 2,488,594 - +1.0 2,488,594 - -1.2 2,488,594 - -1.7 2,488,594 - -1.2 2,488,594 - -1.4 2,488,594 - -0.7 2,488,594 - +1.0 2,488,594 - -2.4 2,488,594 - -1.1 2,488,635 + +0.6 2,488,636 - -1.1 2,488,636 - -1.0 2,488,636 - +1.7 2,488,636 - +0.4 2,488,636 - +0.2 2,488,653 - -0.0 2,488,653 - +0.3 2,488,695 - -0.0 2,488,760 + -1.4 2,488,760 + +1.0 2,488,761 - -0.6 2,488,761 - -0.6 2,488,832 + LRK53_RS11155 0.11 -2.2 2,488,833 - LRK53_RS11155 0.11 -1.4 2,488,907 + LRK53_RS11155 0.16 -1.0 2,488,907 + LRK53_RS11155 0.16 -0.0 2,488,907 + LRK53_RS11155 0.16 -1.4 2,488,907 + LRK53_RS11155 0.16 -0.6 2,488,907 + LRK53_RS11155 0.16 -0.6 2,488,908 - LRK53_RS11155 0.16 +1.0 2,488,908 - LRK53_RS11155 0.16 -0.0 2,488,970 + LRK53_RS11155 0.20 -1.0 2,488,970 + LRK53_RS11155 0.20 -1.6 2,488,970 + LRK53_RS11155 0.20 -0.0 2,488,970 + LRK53_RS11155 0.20 -0.6 2,488,971 - LRK53_RS11155 0.20 -0.0 2,488,971 - LRK53_RS11155 0.20 -0.0 2,489,033 + LRK53_RS11155 0.24 -2.2 2,489,034 - LRK53_RS11155 0.24 +0.6 2,489,216 + LRK53_RS11155 0.35 -1.4 2,489,360 + LRK53_RS11155 0.45 -1.8 2,489,360 + LRK53_RS11155 0.45 -0.0 2,489,360 + LRK53_RS11155 0.45 -0.6 2,489,361 - LRK53_RS11155 0.45 -1.8 2,489,361 - LRK53_RS11155 0.45 -2.0 2,489,697 + LRK53_RS11155 0.66 -0.0 2,489,697 + LRK53_RS11155 0.66 -0.0 2,489,698 - LRK53_RS11155 0.66 -1.6 2,489,698 - LRK53_RS11155 0.66 -1.4 2,489,698 - LRK53_RS11155 0.66 -0.4 2,489,729 + LRK53_RS11155 0.68 -0.4 2,489,730 - LRK53_RS11155 0.68 -1.0 2,489,837 + LRK53_RS11155 0.74 -1.0 2,489,839 + LRK53_RS11155 0.75 -0.0 2,489,839 + LRK53_RS11155 0.75 +1.0 2,489,839 + LRK53_RS11155 0.75 -0.6 2,489,839 + LRK53_RS11155 0.75 -0.0 2,489,839 + LRK53_RS11155 0.75 -1.4 2,489,839 + LRK53_RS11155 0.75 -2.2 2,489,839 + LRK53_RS11155 0.75 +1.0 2,489,839 + LRK53_RS11155 0.75 -0.0 2,489,839 + LRK53_RS11155 0.75 -1.4 2,489,839 + LRK53_RS11155 0.75 -1.8 2,489,839 + LRK53_RS11155 0.75 -0.0 2,489,839 + LRK53_RS11155 0.75 -1.0 2,489,839 + LRK53_RS11155 0.75 -0.6 2,489,839 + LRK53_RS11155 0.75 -0.6 2,489,839 + LRK53_RS11155 0.75 -1.4 2,489,839 + LRK53_RS11155 0.75 -0.8 2,489,839 + LRK53_RS11155 0.75 -2.0 2,489,839 + LRK53_RS11155 0.75 -1.6 2,489,839 + LRK53_RS11155 0.75 -1.0 2,489,839 + LRK53_RS11155 0.75 +0.6 2,489,839 + LRK53_RS11155 0.75 -1.0 2,489,839 + LRK53_RS11155 0.75 -2.2 2,489,839 + LRK53_RS11155 0.75 -2.0 2,489,839 + LRK53_RS11155 0.75 -0.0 2,489,839 + LRK53_RS11155 0.75 -0.4 2,489,839 + LRK53_RS11155 0.75 +1.0 2,489,839 + LRK53_RS11155 0.75 -2.2 2,489,839 + LRK53_RS11155 0.75 -1.4 2,489,839 + LRK53_RS11155 0.75 -0.0 2,489,839 + LRK53_RS11155 0.75 -1.4 2,489,840 - LRK53_RS11155 0.75 +1.0 2,489,840 - LRK53_RS11155 0.75 -0.4 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,840 - LRK53_RS11155 0.75 +1.0 2,489,840 - LRK53_RS11155 0.75 -1.6 2,489,840 - LRK53_RS11155 0.75 -0.0 2,489,840 - LRK53_RS11155 0.75 -2.2 2,489,840 - LRK53_RS11155 0.75 -1.0 2,489,840 - LRK53_RS11155 0.75 +1.0 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,840 - LRK53_RS11155 0.75 -0.0 2,489,840 - LRK53_RS11155 0.75 -1.0 2,489,840 - LRK53_RS11155 0.75 -1.6 2,489,840 - LRK53_RS11155 0.75 -1.0 2,489,840 - LRK53_RS11155 0.75 -1.7 2,489,840 - LRK53_RS11155 0.75 -0.0 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,840 - LRK53_RS11155 0.75 -1.6 2,489,840 - LRK53_RS11155 0.75 +0.4 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,840 - LRK53_RS11155 0.75 -1.5 2,489,840 - LRK53_RS11155 0.75 -1.6 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,840 - LRK53_RS11155 0.75 -2.0 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,840 - LRK53_RS11155 0.75 -0.6 2,489,994 + LRK53_RS11155 0.84 -0.6 2,489,994 + LRK53_RS11155 0.84 -1.6 2,489,994 + LRK53_RS11155 0.84 -1.4 2,489,994 + LRK53_RS11155 0.84 -0.6 2,489,995 - LRK53_RS11155 0.84 -0.0 2,489,995 - LRK53_RS11155 0.84 +1.0 2,490,149 + -0.6 2,490,149 + -1.4 2,490,149 + -2.4 2,490,150 - -1.8 2,490,150 - -2.2 2,490,191 + -1.6 2,490,191 + -1.0 2,490,191 + -1.0 2,490,191 + -2.2 2,490,191 + +1.0 2,490,192 - -2.4 2,490,192 - -0.6 2,490,192 - -2.9 2,490,192 - -2.0 2,490,192 - -0.0 2,490,192 - -1.0 2,490,275 + -1.4 2,490,276 - -0.3 2,490,276 - +1.0 2,490,276 - -0.7 2,490,276 - -0.6 2,490,276 - -0.0 2,490,276 - -1.4 2,490,276 - -0.4 2,490,276 - -0.6 2,490,363 + -1.8 2,490,363 + -1.4 2,490,363 + -1.6 2,490,363 + -0.0 2,490,410 + -0.6 2,490,410 + -1.0 2,490,410 + -0.6 2,490,410 + -1.4 2,490,411 - -1.5 2,490,411 - -1.0 2,490,426 - -0.0
Or see this region's nucleotide sequence