Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS11070 and LRK53_RS11075 are separated by 1 nucleotides LRK53_RS11075 and LRK53_RS11080 overlap by 4 nucleotides LRK53_RS11080 and LRK53_RS11085 overlap by 4 nucleotides
LRK53_RS11070: LRK53_RS11070 - MBL fold metallo-hydrolase, at 2,472,539 to 2,473,924
_RS11070
LRK53_RS11075: LRK53_RS11075 - thymidine phosphorylase family protein, at 2,473,926 to 2,475,440
_RS11075
LRK53_RS11080: LRK53_RS11080 - ribose-phosphate diphosphokinase, at 2,475,437 to 2,476,348
_RS11080
LRK53_RS11085: LRK53_RS11085 - hypothetical protein, at 2,476,345 to 2,477,307
_RS11085
Position (kb)
2473
2474
2475
2476 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2472.929 kb on + strand, within LRK53_RS11070 at 2472.929 kb on + strand, within LRK53_RS11070 at 2472.980 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.002 kb on + strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.003 kb on - strand, within LRK53_RS11070 at 2473.026 kb on - strand, within LRK53_RS11070 at 2473.026 kb on - strand, within LRK53_RS11070 at 2473.138 kb on + strand, within LRK53_RS11070 at 2473.161 kb on + strand, within LRK53_RS11070 at 2473.161 kb on + strand, within LRK53_RS11070 at 2473.161 kb on + strand, within LRK53_RS11070 at 2473.161 kb on + strand, within LRK53_RS11070 at 2473.161 kb on + strand, within LRK53_RS11070 at 2473.162 kb on - strand, within LRK53_RS11070 at 2473.162 kb on - strand, within LRK53_RS11070 at 2473.162 kb on - strand, within LRK53_RS11070 at 2473.162 kb on - strand, within LRK53_RS11070 at 2473.162 kb on - strand, within LRK53_RS11070 at 2473.181 kb on + strand, within LRK53_RS11070 at 2473.181 kb on + strand, within LRK53_RS11070 at 2473.182 kb on - strand, within LRK53_RS11070 at 2473.428 kb on + strand, within LRK53_RS11070 at 2473.428 kb on + strand, within LRK53_RS11070 at 2473.428 kb on + strand, within LRK53_RS11070 at 2473.428 kb on + strand, within LRK53_RS11070 at 2473.429 kb on - strand, within LRK53_RS11070 at 2473.429 kb on - strand, within LRK53_RS11070 at 2473.454 kb on + strand, within LRK53_RS11070 at 2473.454 kb on + strand, within LRK53_RS11070 at 2473.454 kb on + strand, within LRK53_RS11070 at 2473.455 kb on - strand, within LRK53_RS11070 at 2473.455 kb on - strand, within LRK53_RS11070 at 2473.455 kb on - strand, within LRK53_RS11070 at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.802 kb on + strand at 2473.803 kb on - strand at 2473.803 kb on - strand at 2473.803 kb on - strand at 2473.803 kb on - strand at 2473.803 kb on - strand at 2473.803 kb on - strand at 2473.980 kb on + strand at 2473.980 kb on + strand at 2473.980 kb on + strand at 2473.980 kb on + strand at 2473.980 kb on + strand at 2473.981 kb on - strand at 2473.981 kb on - strand at 2473.981 kb on - strand at 2473.981 kb on - strand at 2473.981 kb on - strand at 2473.981 kb on - strand at 2473.981 kb on - strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.998 kb on + strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2473.999 kb on - strand at 2474.242 kb on + strand, within LRK53_RS11075 at 2474.242 kb on + strand, within LRK53_RS11075 at 2474.242 kb on + strand, within LRK53_RS11075 at 2474.242 kb on + strand, within LRK53_RS11075 at 2474.242 kb on + strand, within LRK53_RS11075 at 2474.242 kb on + strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.243 kb on - strand, within LRK53_RS11075 at 2474.518 kb on - strand, within LRK53_RS11075 at 2475.184 kb on - strand, within LRK53_RS11075 at 2475.325 kb on + strand at 2475.381 kb on + strand at 2475.393 kb on + strand at 2475.393 kb on + strand at 2475.393 kb on + strand at 2475.394 kb on - strand at 2475.394 kb on - strand at 2475.394 kb on - strand at 2475.436 kb on + strand at 2475.436 kb on + strand at 2475.436 kb on + strand at 2475.436 kb on + strand at 2475.436 kb on + strand at 2475.437 kb on - strand at 2475.437 kb on - strand at 2475.448 kb on - strand at 2475.702 kb on - strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.710 kb on + strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.711 kb on - strand, within LRK53_RS11080 at 2475.846 kb on + strand, within LRK53_RS11080 at 2475.846 kb on + strand, within LRK53_RS11080 at 2475.846 kb on + strand, within LRK53_RS11080 at 2475.847 kb on - strand, within LRK53_RS11080 at 2475.847 kb on - strand, within LRK53_RS11080 at 2475.847 kb on - strand, within LRK53_RS11080 at 2475.848 kb on + strand, within LRK53_RS11080 at 2475.849 kb on - strand, within LRK53_RS11080 at 2475.849 kb on - strand, within LRK53_RS11080 at 2475.992 kb on + strand, within LRK53_RS11080 at 2475.992 kb on + strand, within LRK53_RS11080 at 2475.992 kb on + strand, within LRK53_RS11080 at 2475.993 kb on - strand, within LRK53_RS11080 at 2475.993 kb on - strand, within LRK53_RS11080 at 2475.993 kb on - strand, within LRK53_RS11080 at 2476.026 kb on - strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.032 kb on + strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.033 kb on - strand, within LRK53_RS11080 at 2476.112 kb on - strand, within LRK53_RS11080 at 2476.137 kb on + strand, within LRK53_RS11080 at 2476.137 kb on + strand, within LRK53_RS11080 at 2476.137 kb on + strand, within LRK53_RS11080 at 2476.137 kb on + strand, within LRK53_RS11080 at 2476.202 kb on + strand, within LRK53_RS11080 at 2476.202 kb on + strand, within LRK53_RS11080 at 2476.203 kb on - strand, within LRK53_RS11080 at 2476.203 kb on - strand, within LRK53_RS11080 at 2476.212 kb on + strand, within LRK53_RS11080 at 2476.270 kb on + strand at 2476.270 kb on + strand at 2476.270 kb on + strand at 2476.270 kb on + strand at 2476.271 kb on - strand at 2476.428 kb on + strand at 2476.429 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,472,929 + LRK53_RS11070 0.28 -1.0 2,472,929 + LRK53_RS11070 0.28 +0.4 2,472,980 + LRK53_RS11070 0.32 -1.0 2,473,002 + LRK53_RS11070 0.33 -0.6 2,473,002 + LRK53_RS11070 0.33 -0.5 2,473,002 + LRK53_RS11070 0.33 -0.6 2,473,002 + LRK53_RS11070 0.33 +0.5 2,473,002 + LRK53_RS11070 0.33 -1.4 2,473,002 + LRK53_RS11070 0.33 -0.5 2,473,002 + LRK53_RS11070 0.33 -1.8 2,473,002 + LRK53_RS11070 0.33 +1.0 2,473,003 - LRK53_RS11070 0.33 -0.7 2,473,003 - LRK53_RS11070 0.33 -0.6 2,473,003 - LRK53_RS11070 0.33 -0.5 2,473,003 - LRK53_RS11070 0.33 -1.0 2,473,003 - LRK53_RS11070 0.33 -0.6 2,473,003 - LRK53_RS11070 0.33 +1.0 2,473,003 - LRK53_RS11070 0.33 -1.2 2,473,003 - LRK53_RS11070 0.33 -0.6 2,473,026 - LRK53_RS11070 0.35 -0.0 2,473,026 - LRK53_RS11070 0.35 -2.5 2,473,138 + LRK53_RS11070 0.43 +0.4 2,473,161 + LRK53_RS11070 0.45 -0.5 2,473,161 + LRK53_RS11070 0.45 -1.7 2,473,161 + LRK53_RS11070 0.45 -0.6 2,473,161 + LRK53_RS11070 0.45 -1.4 2,473,161 + LRK53_RS11070 0.45 -0.0 2,473,162 - LRK53_RS11070 0.45 -2.9 2,473,162 - LRK53_RS11070 0.45 +0.5 2,473,162 - LRK53_RS11070 0.45 +1.0 2,473,162 - LRK53_RS11070 0.45 -0.8 2,473,162 - LRK53_RS11070 0.45 -0.3 2,473,181 + LRK53_RS11070 0.46 -0.0 2,473,181 + LRK53_RS11070 0.46 -0.0 2,473,182 - LRK53_RS11070 0.46 +1.0 2,473,428 + LRK53_RS11070 0.64 +1.0 2,473,428 + LRK53_RS11070 0.64 -0.5 2,473,428 + LRK53_RS11070 0.64 -0.5 2,473,428 + LRK53_RS11070 0.64 -0.0 2,473,429 - LRK53_RS11070 0.64 -0.0 2,473,429 - LRK53_RS11070 0.64 -0.6 2,473,454 + LRK53_RS11070 0.66 +0.3 2,473,454 + LRK53_RS11070 0.66 -1.0 2,473,454 + LRK53_RS11070 0.66 -1.1 2,473,455 - LRK53_RS11070 0.66 -0.8 2,473,455 - LRK53_RS11070 0.66 +1.0 2,473,455 - LRK53_RS11070 0.66 +0.3 2,473,802 + -0.6 2,473,802 + -0.4 2,473,802 + -1.0 2,473,802 + -1.0 2,473,802 + -2.2 2,473,802 + -0.3 2,473,802 + +0.6 2,473,802 + +0.2 2,473,802 + +0.6 2,473,803 - +2.0 2,473,803 - -0.0 2,473,803 - -0.8 2,473,803 - -0.7 2,473,803 - -1.4 2,473,803 - -0.0 2,473,980 + -0.3 2,473,980 + -1.1 2,473,980 + +0.4 2,473,980 + -1.0 2,473,980 + +0.5 2,473,981 - +0.1 2,473,981 - -0.3 2,473,981 - +1.5 2,473,981 - -0.6 2,473,981 - +1.0 2,473,981 - -2.2 2,473,981 - -0.3 2,473,998 + +0.4 2,473,998 + -0.6 2,473,998 + -0.9 2,473,998 + -0.9 2,473,998 + -0.5 2,473,998 + -1.4 2,473,998 + -2.0 2,473,998 + +0.2 2,473,998 + -0.4 2,473,998 + -0.7 2,473,998 + -0.9 2,473,998 + +2.0 2,473,999 - -0.9 2,473,999 - +1.0 2,473,999 - +1.0 2,473,999 - -1.6 2,473,999 - -0.6 2,473,999 - +1.0 2,473,999 - +1.5 2,473,999 - +0.4 2,473,999 - -0.0 2,473,999 - -0.8 2,473,999 - -1.4 2,473,999 - +1.0 2,473,999 - -0.6 2,473,999 - -0.0 2,473,999 - +0.4 2,473,999 - +0.5 2,474,242 + LRK53_RS11075 0.21 -1.4 2,474,242 + LRK53_RS11075 0.21 -2.6 2,474,242 + LRK53_RS11075 0.21 +1.0 2,474,242 + LRK53_RS11075 0.21 -1.4 2,474,242 + LRK53_RS11075 0.21 -0.0 2,474,242 + LRK53_RS11075 0.21 -1.5 2,474,243 - LRK53_RS11075 0.21 -2.1 2,474,243 - LRK53_RS11075 0.21 -0.6 2,474,243 - LRK53_RS11075 0.21 -1.0 2,474,243 - LRK53_RS11075 0.21 -0.6 2,474,243 - LRK53_RS11075 0.21 -0.0 2,474,243 - LRK53_RS11075 0.21 -0.8 2,474,243 - LRK53_RS11075 0.21 -1.0 2,474,518 - LRK53_RS11075 0.39 -0.6 2,475,184 - LRK53_RS11075 0.83 +0.5 2,475,325 + -0.0 2,475,381 + -0.6 2,475,393 + -1.2 2,475,393 + -2.0 2,475,393 + -1.4 2,475,394 - -0.0 2,475,394 - +0.4 2,475,394 - -1.4 2,475,436 + -0.0 2,475,436 + +0.2 2,475,436 + -1.6 2,475,436 + +1.0 2,475,436 + -1.6 2,475,437 - -1.0 2,475,437 - +2.0 2,475,448 - +1.0 2,475,702 - LRK53_RS11080 0.29 +1.0 2,475,710 + LRK53_RS11080 0.30 -1.4 2,475,710 + LRK53_RS11080 0.30 -0.4 2,475,710 + LRK53_RS11080 0.30 -1.6 2,475,710 + LRK53_RS11080 0.30 -0.3 2,475,710 + LRK53_RS11080 0.30 +0.2 2,475,710 + LRK53_RS11080 0.30 +0.6 2,475,710 + LRK53_RS11080 0.30 -0.9 2,475,710 + LRK53_RS11080 0.30 +0.3 2,475,710 + LRK53_RS11080 0.30 +0.4 2,475,710 + LRK53_RS11080 0.30 +1.0 2,475,710 + LRK53_RS11080 0.30 +0.6 2,475,710 + LRK53_RS11080 0.30 -1.1 2,475,711 - LRK53_RS11080 0.30 -0.2 2,475,711 - LRK53_RS11080 0.30 -0.4 2,475,711 - LRK53_RS11080 0.30 -0.0 2,475,711 - LRK53_RS11080 0.30 -0.8 2,475,711 - LRK53_RS11080 0.30 +0.4 2,475,711 - LRK53_RS11080 0.30 -0.8 2,475,711 - LRK53_RS11080 0.30 -0.0 2,475,711 - LRK53_RS11080 0.30 +0.3 2,475,711 - LRK53_RS11080 0.30 +1.0 2,475,711 - LRK53_RS11080 0.30 +1.3 2,475,711 - LRK53_RS11080 0.30 -0.6 2,475,711 - LRK53_RS11080 0.30 -1.4 2,475,711 - LRK53_RS11080 0.30 -0.8 2,475,711 - LRK53_RS11080 0.30 -0.9 2,475,711 - LRK53_RS11080 0.30 +2.0 2,475,846 + LRK53_RS11080 0.45 -0.6 2,475,846 + LRK53_RS11080 0.45 +1.0 2,475,846 + LRK53_RS11080 0.45 +0.4 2,475,847 - LRK53_RS11080 0.45 -1.0 2,475,847 - LRK53_RS11080 0.45 -0.0 2,475,847 - LRK53_RS11080 0.45 -0.8 2,475,848 + LRK53_RS11080 0.45 -1.2 2,475,849 - LRK53_RS11080 0.45 +1.0 2,475,849 - LRK53_RS11080 0.45 +0.4 2,475,992 + LRK53_RS11080 0.61 +0.2 2,475,992 + LRK53_RS11080 0.61 +0.2 2,475,992 + LRK53_RS11080 0.61 -0.0 2,475,993 - LRK53_RS11080 0.61 -2.2 2,475,993 - LRK53_RS11080 0.61 +0.4 2,475,993 - LRK53_RS11080 0.61 -0.0 2,476,026 - LRK53_RS11080 0.65 +0.2 2,476,032 + LRK53_RS11080 0.65 -1.0 2,476,032 + LRK53_RS11080 0.65 +2.0 2,476,032 + LRK53_RS11080 0.65 -0.0 2,476,032 + LRK53_RS11080 0.65 +0.2 2,476,032 + LRK53_RS11080 0.65 -0.0 2,476,032 + LRK53_RS11080 0.65 -0.6 2,476,032 + LRK53_RS11080 0.65 -0.6 2,476,032 + LRK53_RS11080 0.65 -0.9 2,476,033 - LRK53_RS11080 0.65 -0.0 2,476,033 - LRK53_RS11080 0.65 -0.0 2,476,033 - LRK53_RS11080 0.65 -0.5 2,476,033 - LRK53_RS11080 0.65 -0.6 2,476,033 - LRK53_RS11080 0.65 -0.3 2,476,033 - LRK53_RS11080 0.65 +0.4 2,476,033 - LRK53_RS11080 0.65 +1.0 2,476,033 - LRK53_RS11080 0.65 -1.4 2,476,112 - LRK53_RS11080 0.74 -0.0 2,476,137 + LRK53_RS11080 0.77 -1.0 2,476,137 + LRK53_RS11080 0.77 -1.0 2,476,137 + LRK53_RS11080 0.77 +0.4 2,476,137 + LRK53_RS11080 0.77 +0.4 2,476,202 + LRK53_RS11080 0.84 -0.6 2,476,202 + LRK53_RS11080 0.84 -0.0 2,476,203 - LRK53_RS11080 0.84 -1.6 2,476,203 - LRK53_RS11080 0.84 +0.2 2,476,212 + LRK53_RS11080 0.85 +1.0 2,476,270 + -0.0 2,476,270 + -0.6 2,476,270 + +0.5 2,476,270 + -1.0 2,476,271 - -0.0 2,476,428 + -1.0 2,476,429 - -1.0
Or see this region's nucleotide sequence