Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS10165 and LRK53_RS10170 are separated by 130 nucleotides LRK53_RS10170 and LRK53_RS10175 are separated by 36 nucleotides LRK53_RS10175 and LRK53_RS10180 overlap by 8 nucleotides
LRK53_RS10165: LRK53_RS10165 - Tn3 family transposase, at 2,270,489 to 2,273,446
_RS10165
LRK53_RS10170: LRK53_RS10170 - hypothetical protein, at 2,273,577 to 2,273,798
_RS10170
LRK53_RS10175: LRK53_RS10175 - DUF3606 domain-containing protein, at 2,273,835 to 2,274,041
_RS10175
LRK53_RS10180: LRK53_RS10180 - peptide chain release factor 3, at 2,274,034 to 2,275,644
_RS10180
Position (kb)
2273
2274
2275 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2272.894 kb on + strand, within LRK53_RS10165 at 2272.981 kb on + strand, within LRK53_RS10165 at 2272.981 kb on + strand, within LRK53_RS10165 at 2272.982 kb on - strand, within LRK53_RS10165 at 2272.982 kb on - strand, within LRK53_RS10165 at 2272.982 kb on - strand, within LRK53_RS10165 at 2273.011 kb on + strand, within LRK53_RS10165 at 2273.012 kb on - strand, within LRK53_RS10165 at 2273.160 kb on + strand at 2273.268 kb on + strand at 2273.268 kb on + strand at 2273.268 kb on + strand at 2273.268 kb on + strand at 2273.269 kb on - strand at 2273.373 kb on + strand at 2273.373 kb on + strand at 2273.373 kb on + strand at 2273.374 kb on - strand at 2273.374 kb on - strand at 2273.422 kb on - strand at 2273.455 kb on - strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.495 kb on + strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.496 kb on - strand at 2273.499 kb on + strand at 2273.601 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.695 kb on + strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.696 kb on - strand, within LRK53_RS10170 at 2273.848 kb on + strand at 2273.848 kb on + strand at 2273.848 kb on + strand at 2273.849 kb on - strand at 2273.849 kb on - strand at 2273.849 kb on - strand at 2273.882 kb on + strand, within LRK53_RS10175 at 2273.882 kb on + strand, within LRK53_RS10175 at 2273.882 kb on + strand, within LRK53_RS10175 at 2273.920 kb on + strand, within LRK53_RS10175 at 2273.920 kb on + strand, within LRK53_RS10175 at 2273.920 kb on + strand, within LRK53_RS10175 at 2273.920 kb on + strand, within LRK53_RS10175 at 2273.920 kb on + strand, within LRK53_RS10175 at 2273.921 kb on - strand, within LRK53_RS10175 at 2273.921 kb on - strand, within LRK53_RS10175 at 2273.921 kb on - strand, within LRK53_RS10175 at 2273.948 kb on - strand, within LRK53_RS10175 at 2273.948 kb on - strand, within LRK53_RS10175 at 2273.971 kb on + strand, within LRK53_RS10175 at 2273.971 kb on + strand, within LRK53_RS10175 at 2273.971 kb on + strand, within LRK53_RS10175 at 2273.971 kb on + strand, within LRK53_RS10175 at 2273.972 kb on - strand, within LRK53_RS10175 at 2273.972 kb on - strand, within LRK53_RS10175 at 2273.992 kb on + strand, within LRK53_RS10175 at 2273.992 kb on + strand, within LRK53_RS10175 at 2273.992 kb on + strand, within LRK53_RS10175 at 2273.992 kb on + strand, within LRK53_RS10175 at 2273.993 kb on - strand, within LRK53_RS10175 at 2273.993 kb on - strand, within LRK53_RS10175 at 2273.993 kb on - strand, within LRK53_RS10175 at 2273.993 kb on - strand, within LRK53_RS10175 at 2274.143 kb on + strand at 2274.143 kb on + strand at 2274.143 kb on + strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.144 kb on - strand at 2274.281 kb on + strand, within LRK53_RS10180 at 2274.282 kb on - strand, within LRK53_RS10180 at 2274.282 kb on - strand, within LRK53_RS10180 at 2274.328 kb on + strand, within LRK53_RS10180 at 2274.328 kb on + strand, within LRK53_RS10180 at 2274.329 kb on - strand, within LRK53_RS10180 at 2274.329 kb on - strand, within LRK53_RS10180 at 2274.329 kb on - strand, within LRK53_RS10180 at 2274.547 kb on - strand, within LRK53_RS10180 at 2274.677 kb on + strand, within LRK53_RS10180 at 2274.678 kb on - strand, within LRK53_RS10180 at 2274.678 kb on - strand, within LRK53_RS10180 at 2274.678 kb on - strand, within LRK53_RS10180 at 2274.722 kb on - strand, within LRK53_RS10180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,272,894 + LRK53_RS10165 0.81 -0.0 2,272,981 + LRK53_RS10165 0.84 +0.2 2,272,981 + LRK53_RS10165 0.84 -1.2 2,272,982 - LRK53_RS10165 0.84 -1.0 2,272,982 - LRK53_RS10165 0.84 -1.0 2,272,982 - LRK53_RS10165 0.84 -1.8 2,273,011 + LRK53_RS10165 0.85 -1.6 2,273,012 - LRK53_RS10165 0.85 +0.4 2,273,160 + -1.0 2,273,268 + -0.2 2,273,268 + +1.0 2,273,268 + +1.0 2,273,268 + -0.4 2,273,269 - -0.4 2,273,373 + -0.9 2,273,373 + -1.0 2,273,373 + -0.6 2,273,374 - -1.2 2,273,374 - +2.0 2,273,422 - +0.1 2,273,455 - -1.0 2,273,495 + -1.2 2,273,495 + -0.6 2,273,495 + -0.4 2,273,495 + -1.6 2,273,495 + -2.0 2,273,495 + +0.1 2,273,495 + -2.5 2,273,495 + -0.6 2,273,496 - -2.9 2,273,496 - -2.0 2,273,496 - -0.6 2,273,496 - -1.0 2,273,496 - +1.0 2,273,496 - -0.0 2,273,496 - -1.6 2,273,496 - -1.6 2,273,496 - -0.6 2,273,499 + -0.8 2,273,601 + LRK53_RS10170 0.11 -0.0 2,273,695 + LRK53_RS10170 0.53 +1.0 2,273,695 + LRK53_RS10170 0.53 -2.5 2,273,695 + LRK53_RS10170 0.53 +0.4 2,273,695 + LRK53_RS10170 0.53 -1.5 2,273,695 + LRK53_RS10170 0.53 +2.5 2,273,695 + LRK53_RS10170 0.53 -0.4 2,273,695 + LRK53_RS10170 0.53 -0.6 2,273,695 + LRK53_RS10170 0.53 +2.5 2,273,695 + LRK53_RS10170 0.53 -0.7 2,273,695 + LRK53_RS10170 0.53 -1.4 2,273,695 + LRK53_RS10170 0.53 -1.6 2,273,695 + LRK53_RS10170 0.53 -1.0 2,273,695 + LRK53_RS10170 0.53 -0.2 2,273,695 + LRK53_RS10170 0.53 -3.6 2,273,695 + LRK53_RS10170 0.53 -0.6 2,273,695 + LRK53_RS10170 0.53 -0.4 2,273,695 + LRK53_RS10170 0.53 -0.3 2,273,695 + LRK53_RS10170 0.53 +1.0 2,273,695 + LRK53_RS10170 0.53 -0.4 2,273,695 + LRK53_RS10170 0.53 -0.3 2,273,695 + LRK53_RS10170 0.53 -0.6 2,273,695 + LRK53_RS10170 0.53 -0.2 2,273,695 + LRK53_RS10170 0.53 -0.2 2,273,695 + LRK53_RS10170 0.53 -0.6 2,273,695 + LRK53_RS10170 0.53 -0.0 2,273,695 + LRK53_RS10170 0.53 -0.0 2,273,696 - LRK53_RS10170 0.54 -0.6 2,273,696 - LRK53_RS10170 0.54 +1.0 2,273,696 - LRK53_RS10170 0.54 -0.0 2,273,696 - LRK53_RS10170 0.54 -0.0 2,273,696 - LRK53_RS10170 0.54 -0.4 2,273,696 - LRK53_RS10170 0.54 -1.8 2,273,696 - LRK53_RS10170 0.54 -0.0 2,273,696 - LRK53_RS10170 0.54 +0.4 2,273,696 - LRK53_RS10170 0.54 -1.0 2,273,696 - LRK53_RS10170 0.54 -1.4 2,273,696 - LRK53_RS10170 0.54 -1.6 2,273,696 - LRK53_RS10170 0.54 +1.0 2,273,696 - LRK53_RS10170 0.54 -0.6 2,273,696 - LRK53_RS10170 0.54 -1.4 2,273,696 - LRK53_RS10170 0.54 -0.0 2,273,696 - LRK53_RS10170 0.54 +1.0 2,273,696 - LRK53_RS10170 0.54 -0.3 2,273,696 - LRK53_RS10170 0.54 +1.0 2,273,696 - LRK53_RS10170 0.54 -1.7 2,273,696 - LRK53_RS10170 0.54 +1.0 2,273,696 - LRK53_RS10170 0.54 -1.0 2,273,696 - LRK53_RS10170 0.54 -0.6 2,273,696 - LRK53_RS10170 0.54 +1.0 2,273,696 - LRK53_RS10170 0.54 -1.4 2,273,696 - LRK53_RS10170 0.54 -0.8 2,273,696 - LRK53_RS10170 0.54 -1.2 2,273,696 - LRK53_RS10170 0.54 -1.4 2,273,696 - LRK53_RS10170 0.54 -1.0 2,273,696 - LRK53_RS10170 0.54 +0.2 2,273,696 - LRK53_RS10170 0.54 -1.0 2,273,696 - LRK53_RS10170 0.54 -1.5 2,273,696 - LRK53_RS10170 0.54 -1.0 2,273,696 - LRK53_RS10170 0.54 -1.5 2,273,696 - LRK53_RS10170 0.54 -0.0 2,273,696 - LRK53_RS10170 0.54 -1.4 2,273,696 - LRK53_RS10170 0.54 -1.6 2,273,848 + +1.0 2,273,848 + -0.4 2,273,848 + +0.2 2,273,849 - -0.0 2,273,849 - -1.6 2,273,849 - -0.6 2,273,882 + LRK53_RS10175 0.23 -1.6 2,273,882 + LRK53_RS10175 0.23 +1.0 2,273,882 + LRK53_RS10175 0.23 -0.8 2,273,920 + LRK53_RS10175 0.41 -0.9 2,273,920 + LRK53_RS10175 0.41 -0.0 2,273,920 + LRK53_RS10175 0.41 +0.4 2,273,920 + LRK53_RS10175 0.41 -1.6 2,273,920 + LRK53_RS10175 0.41 +0.4 2,273,921 - LRK53_RS10175 0.42 -1.0 2,273,921 - LRK53_RS10175 0.42 -0.3 2,273,921 - LRK53_RS10175 0.42 +0.5 2,273,948 - LRK53_RS10175 0.55 -0.6 2,273,948 - LRK53_RS10175 0.55 -1.4 2,273,971 + LRK53_RS10175 0.66 -0.6 2,273,971 + LRK53_RS10175 0.66 -0.6 2,273,971 + LRK53_RS10175 0.66 -1.4 2,273,971 + LRK53_RS10175 0.66 -1.4 2,273,972 - LRK53_RS10175 0.66 -3.1 2,273,972 - LRK53_RS10175 0.66 -2.2 2,273,992 + LRK53_RS10175 0.76 -1.5 2,273,992 + LRK53_RS10175 0.76 -0.5 2,273,992 + LRK53_RS10175 0.76 -0.2 2,273,992 + LRK53_RS10175 0.76 -0.0 2,273,993 - LRK53_RS10175 0.76 -0.0 2,273,993 - LRK53_RS10175 0.76 -0.6 2,273,993 - LRK53_RS10175 0.76 -0.0 2,273,993 - LRK53_RS10175 0.76 -0.4 2,274,143 + -0.7 2,274,143 + -1.3 2,274,143 + -1.2 2,274,144 - -1.5 2,274,144 - -1.2 2,274,144 - +0.5 2,274,144 - +0.8 2,274,144 - -0.0 2,274,144 - -0.9 2,274,144 - -0.6 2,274,144 - -1.0 2,274,144 - -1.6 2,274,281 + LRK53_RS10180 0.15 -1.8 2,274,282 - LRK53_RS10180 0.15 +2.0 2,274,282 - LRK53_RS10180 0.15 -2.4 2,274,328 + LRK53_RS10180 0.18 -1.3 2,274,328 + LRK53_RS10180 0.18 -0.6 2,274,329 - LRK53_RS10180 0.18 +3.3 2,274,329 - LRK53_RS10180 0.18 -0.0 2,274,329 - LRK53_RS10180 0.18 +0.2 2,274,547 - LRK53_RS10180 0.32 +0.4 2,274,677 + LRK53_RS10180 0.40 -1.4 2,274,678 - LRK53_RS10180 0.40 +2.0 2,274,678 - LRK53_RS10180 0.40 -0.8 2,274,678 - LRK53_RS10180 0.40 -0.5 2,274,722 - LRK53_RS10180 0.43 -0.6
Or see this region's nucleotide sequence