Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS10030 and LRK53_RS10035 are separated by 106 nucleotides LRK53_RS10035 and LRK53_RS10040 are separated by 248 nucleotides LRK53_RS10040 and LRK53_RS10045 are separated by 9 nucleotides
LRK53_RS10030: LRK53_RS10030 - patatin-like phospholipase family protein, at 2,244,832 to 2,246,379
_RS10030
LRK53_RS10035: LRK53_RS10035 - hypothetical protein, at 2,246,486 to 2,246,908
_RS10035
LRK53_RS10040: LRK53_RS10040 - DUF2282 domain-containing protein, at 2,247,157 to 2,247,453
_RS10040
LRK53_RS10045: LRK53_RS10045 - DUF692 domain-containing protein, at 2,247,463 to 2,248,329
_RS10045
Position (kb)
2246
2247 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2245.583 kb on - strand, within LRK53_RS10030 at 2245.654 kb on + strand, within LRK53_RS10030 at 2245.654 kb on + strand, within LRK53_RS10030 at 2245.655 kb on - strand, within LRK53_RS10030 at 2245.655 kb on - strand, within LRK53_RS10030 at 2245.655 kb on - strand, within LRK53_RS10030 at 2245.655 kb on - strand, within LRK53_RS10030 at 2245.655 kb on - strand, within LRK53_RS10030 at 2245.756 kb on + strand, within LRK53_RS10030 at 2245.756 kb on + strand, within LRK53_RS10030 at 2245.756 kb on + strand, within LRK53_RS10030 at 2245.757 kb on - strand, within LRK53_RS10030 at 2245.757 kb on - strand, within LRK53_RS10030 at 2245.823 kb on + strand, within LRK53_RS10030 at 2245.855 kb on + strand, within LRK53_RS10030 at 2245.856 kb on - strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.901 kb on + strand, within LRK53_RS10030 at 2245.902 kb on - strand, within LRK53_RS10030 at 2245.902 kb on - strand, within LRK53_RS10030 at 2245.902 kb on - strand, within LRK53_RS10030 at 2245.902 kb on - strand, within LRK53_RS10030 at 2245.902 kb on - strand, within LRK53_RS10030 at 2245.902 kb on - strand, within LRK53_RS10030 at 2245.948 kb on + strand, within LRK53_RS10030 at 2245.948 kb on + strand, within LRK53_RS10030 at 2245.948 kb on + strand, within LRK53_RS10030 at 2245.949 kb on - strand, within LRK53_RS10030 at 2245.949 kb on - strand, within LRK53_RS10030 at 2245.949 kb on - strand, within LRK53_RS10030 at 2245.949 kb on - strand, within LRK53_RS10030 at 2245.949 kb on - strand, within LRK53_RS10030 at 2245.957 kb on + strand, within LRK53_RS10030 at 2245.958 kb on - strand, within LRK53_RS10030 at 2245.998 kb on + strand, within LRK53_RS10030 at 2245.998 kb on + strand, within LRK53_RS10030 at 2245.999 kb on - strand, within LRK53_RS10030 at 2246.033 kb on + strand, within LRK53_RS10030 at 2246.033 kb on + strand, within LRK53_RS10030 at 2246.033 kb on + strand, within LRK53_RS10030 at 2246.033 kb on + strand, within LRK53_RS10030 at 2246.034 kb on - strand, within LRK53_RS10030 at 2246.034 kb on - strand, within LRK53_RS10030 at 2246.034 kb on - strand, within LRK53_RS10030 at 2246.034 kb on - strand, within LRK53_RS10030 at 2246.453 kb on - strand at 2246.640 kb on - strand, within LRK53_RS10035 at 2246.852 kb on + strand, within LRK53_RS10035 at 2247.080 kb on + strand at 2247.081 kb on - strand at 2247.081 kb on - strand at 2247.081 kb on - strand at 2247.081 kb on - strand at 2247.081 kb on - strand at 2247.081 kb on - strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.112 kb on + strand at 2247.113 kb on - strand at 2247.113 kb on - strand at 2247.113 kb on - strand at 2247.113 kb on - strand at 2247.113 kb on - strand at 2247.113 kb on - strand at 2247.113 kb on - strand at 2247.142 kb on - strand at 2247.142 kb on - strand at 2247.142 kb on - strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.149 kb on + strand at 2247.150 kb on - strand at 2247.150 kb on - strand at 2247.163 kb on + strand at 2247.163 kb on + strand at 2247.163 kb on + strand at 2247.163 kb on + strand at 2247.164 kb on - strand at 2247.164 kb on - strand at 2247.292 kb on + strand, within LRK53_RS10040 at 2247.293 kb on - strand, within LRK53_RS10040 at 2247.293 kb on - strand, within LRK53_RS10040 at 2247.376 kb on - strand, within LRK53_RS10040 at 2247.585 kb on + strand, within LRK53_RS10045 at 2247.585 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.595 kb on + strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.596 kb on - strand, within LRK53_RS10045 at 2247.649 kb on + strand, within LRK53_RS10045 at 2247.650 kb on - strand, within LRK53_RS10045 at 2247.815 kb on + strand, within LRK53_RS10045 at 2247.826 kb on + strand, within LRK53_RS10045 at 2247.826 kb on + strand, within LRK53_RS10045 at 2247.826 kb on + strand, within LRK53_RS10045 at 2247.826 kb on + strand, within LRK53_RS10045 at 2247.827 kb on - strand, within LRK53_RS10045 at 2247.828 kb on + strand, within LRK53_RS10045 at 2247.828 kb on + strand, within LRK53_RS10045 at 2247.828 kb on + strand, within LRK53_RS10045 at 2247.828 kb on + strand, within LRK53_RS10045 at 2247.828 kb on + strand, within LRK53_RS10045 at 2247.829 kb on - strand, within LRK53_RS10045 at 2247.829 kb on - strand, within LRK53_RS10045 at 2247.829 kb on - strand, within LRK53_RS10045 at 2247.829 kb on - strand, within LRK53_RS10045 at 2247.829 kb on - strand, within LRK53_RS10045 at 2247.829 kb on - strand, within LRK53_RS10045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,245,583 - LRK53_RS10030 0.49 -2.0 2,245,654 + LRK53_RS10030 0.53 -0.0 2,245,654 + LRK53_RS10030 0.53 -1.6 2,245,655 - LRK53_RS10030 0.53 -1.0 2,245,655 - LRK53_RS10030 0.53 +0.6 2,245,655 - LRK53_RS10030 0.53 -0.2 2,245,655 - LRK53_RS10030 0.53 -0.6 2,245,655 - LRK53_RS10030 0.53 +1.0 2,245,756 + LRK53_RS10030 0.60 -0.6 2,245,756 + LRK53_RS10030 0.60 -3.7 2,245,756 + LRK53_RS10030 0.60 -0.0 2,245,757 - LRK53_RS10030 0.60 -1.8 2,245,757 - LRK53_RS10030 0.60 -0.0 2,245,823 + LRK53_RS10030 0.64 +1.2 2,245,855 + LRK53_RS10030 0.66 -1.0 2,245,856 - LRK53_RS10030 0.66 +0.1 2,245,901 + LRK53_RS10030 0.69 -0.0 2,245,901 + LRK53_RS10030 0.69 +0.4 2,245,901 + LRK53_RS10030 0.69 -0.3 2,245,901 + LRK53_RS10030 0.69 -0.4 2,245,901 + LRK53_RS10030 0.69 +1.0 2,245,901 + LRK53_RS10030 0.69 +0.4 2,245,901 + LRK53_RS10030 0.69 -1.0 2,245,902 - LRK53_RS10030 0.69 -3.0 2,245,902 - LRK53_RS10030 0.69 +1.1 2,245,902 - LRK53_RS10030 0.69 -0.6 2,245,902 - LRK53_RS10030 0.69 -0.6 2,245,902 - LRK53_RS10030 0.69 -0.4 2,245,902 - LRK53_RS10030 0.69 +0.4 2,245,948 + LRK53_RS10030 0.72 -0.0 2,245,948 + LRK53_RS10030 0.72 -0.0 2,245,948 + LRK53_RS10030 0.72 -0.4 2,245,949 - LRK53_RS10030 0.72 -0.5 2,245,949 - LRK53_RS10030 0.72 -1.4 2,245,949 - LRK53_RS10030 0.72 -1.0 2,245,949 - LRK53_RS10030 0.72 -0.7 2,245,949 - LRK53_RS10030 0.72 -0.5 2,245,957 + LRK53_RS10030 0.73 -1.0 2,245,958 - LRK53_RS10030 0.73 +0.2 2,245,998 + LRK53_RS10030 0.75 -1.6 2,245,998 + LRK53_RS10030 0.75 +0.2 2,245,999 - LRK53_RS10030 0.75 +0.3 2,246,033 + LRK53_RS10030 0.78 -1.0 2,246,033 + LRK53_RS10030 0.78 -1.4 2,246,033 + LRK53_RS10030 0.78 -1.0 2,246,033 + LRK53_RS10030 0.78 -0.0 2,246,034 - LRK53_RS10030 0.78 -0.0 2,246,034 - LRK53_RS10030 0.78 -3.0 2,246,034 - LRK53_RS10030 0.78 -1.1 2,246,034 - LRK53_RS10030 0.78 -1.3 2,246,453 - -1.8 2,246,640 - LRK53_RS10035 0.36 -1.2 2,246,852 + LRK53_RS10035 0.87 -2.2 2,247,080 + -0.3 2,247,081 - -0.4 2,247,081 - +0.4 2,247,081 - +0.2 2,247,081 - -0.9 2,247,081 - +0.4 2,247,081 - -0.5 2,247,112 + -0.6 2,247,112 + -1.6 2,247,112 + -0.0 2,247,112 + -0.0 2,247,112 + -0.8 2,247,112 + +1.0 2,247,112 + -0.0 2,247,112 + -0.8 2,247,112 + -1.4 2,247,113 - -0.6 2,247,113 - -1.8 2,247,113 - -0.6 2,247,113 - -0.0 2,247,113 - +0.4 2,247,113 - +1.0 2,247,113 - -1.0 2,247,142 - -0.2 2,247,142 - +0.4 2,247,142 - -0.8 2,247,149 + -0.6 2,247,149 + -0.5 2,247,149 + -0.8 2,247,149 + -1.3 2,247,149 + -1.0 2,247,149 + +1.0 2,247,149 + -1.2 2,247,149 + -2.6 2,247,150 - -0.6 2,247,150 - -1.0 2,247,163 + -0.0 2,247,163 + -1.4 2,247,163 + -0.2 2,247,163 + +1.0 2,247,164 - -1.8 2,247,164 - -0.0 2,247,292 + LRK53_RS10040 0.45 -0.8 2,247,293 - LRK53_RS10040 0.46 -1.5 2,247,293 - LRK53_RS10040 0.46 +0.4 2,247,376 - LRK53_RS10040 0.74 +0.4 2,247,585 + LRK53_RS10045 0.14 -1.0 2,247,585 + LRK53_RS10045 0.14 +1.0 2,247,595 + LRK53_RS10045 0.15 -1.9 2,247,595 + LRK53_RS10045 0.15 -0.6 2,247,595 + LRK53_RS10045 0.15 +1.0 2,247,595 + LRK53_RS10045 0.15 -1.4 2,247,595 + LRK53_RS10045 0.15 -0.0 2,247,595 + LRK53_RS10045 0.15 -0.9 2,247,595 + LRK53_RS10045 0.15 -0.4 2,247,595 + LRK53_RS10045 0.15 +1.0 2,247,596 - LRK53_RS10045 0.15 -0.6 2,247,596 - LRK53_RS10045 0.15 -1.9 2,247,596 - LRK53_RS10045 0.15 -1.0 2,247,596 - LRK53_RS10045 0.15 -1.0 2,247,596 - LRK53_RS10045 0.15 -0.0 2,247,596 - LRK53_RS10045 0.15 -0.6 2,247,596 - LRK53_RS10045 0.15 -0.0 2,247,596 - LRK53_RS10045 0.15 -0.6 2,247,596 - LRK53_RS10045 0.15 +1.0 2,247,596 - LRK53_RS10045 0.15 -2.0 2,247,649 + LRK53_RS10045 0.21 -0.6 2,247,650 - LRK53_RS10045 0.22 -0.6 2,247,815 + LRK53_RS10045 0.41 -2.4 2,247,826 + LRK53_RS10045 0.42 +1.0 2,247,826 + LRK53_RS10045 0.42 -0.5 2,247,826 + LRK53_RS10045 0.42 -1.2 2,247,826 + LRK53_RS10045 0.42 +1.5 2,247,827 - LRK53_RS10045 0.42 -1.3 2,247,828 + LRK53_RS10045 0.42 -0.3 2,247,828 + LRK53_RS10045 0.42 -1.2 2,247,828 + LRK53_RS10045 0.42 -1.6 2,247,828 + LRK53_RS10045 0.42 -0.9 2,247,828 + LRK53_RS10045 0.42 -1.4 2,247,829 - LRK53_RS10045 0.42 -0.0 2,247,829 - LRK53_RS10045 0.42 +0.4 2,247,829 - LRK53_RS10045 0.42 +0.3 2,247,829 - LRK53_RS10045 0.42 -0.6 2,247,829 - LRK53_RS10045 0.42 +0.4 2,247,829 - LRK53_RS10045 0.42 -1.1
Or see this region's nucleotide sequence