Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09900 and LRK53_RS09905 overlap by 1 nucleotides LRK53_RS09905 and LRK53_RS09910 are separated by 12 nucleotides
LRK53_RS09900: LRK53_RS09900 - transcriptional repressor LexA, at 2,222,999 to 2,223,619
_RS09900
LRK53_RS09905: LRK53_RS09905 - translesion DNA synthesis-associated protein ImuA, at 2,223,619 to 2,224,242
_RS09905
LRK53_RS09910: LRK53_RS09910 - DNA polymerase Y family protein, at 2,224,255 to 2,225,658
_RS09910
Position (kb)
2223
2224
2225 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2222.630 kb on + strand at 2222.631 kb on - strand at 2222.631 kb on - strand at 2222.742 kb on + strand at 2222.748 kb on + strand at 2222.749 kb on - strand at 2222.750 kb on + strand at 2222.751 kb on - strand at 2222.951 kb on - strand at 2222.958 kb on + strand at 2222.975 kb on + strand at 2222.975 kb on + strand at 2222.976 kb on - strand at 2222.976 kb on - strand at 2222.980 kb on - strand at 2222.989 kb on + strand at 2222.989 kb on + strand at 2223.318 kb on + strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.319 kb on - strand, within LRK53_RS09900 at 2223.350 kb on + strand, within LRK53_RS09900 at 2223.350 kb on + strand, within LRK53_RS09900 at 2223.350 kb on + strand, within LRK53_RS09900 at 2223.350 kb on + strand, within LRK53_RS09900 at 2223.351 kb on - strand, within LRK53_RS09900 at 2223.351 kb on - strand, within LRK53_RS09900 at 2223.351 kb on - strand, within LRK53_RS09900 at 2223.497 kb on + strand, within LRK53_RS09900 at 2223.497 kb on + strand, within LRK53_RS09900 at 2223.498 kb on - strand, within LRK53_RS09900 at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.593 kb on + strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.594 kb on - strand at 2223.877 kb on + strand, within LRK53_RS09905 at 2223.877 kb on + strand, within LRK53_RS09905 at 2223.877 kb on + strand, within LRK53_RS09905 at 2223.878 kb on - strand, within LRK53_RS09905 at 2223.878 kb on - strand, within LRK53_RS09905 at 2224.422 kb on + strand, within LRK53_RS09910 at 2224.578 kb on + strand, within LRK53_RS09910 at 2224.578 kb on + strand, within LRK53_RS09910 at 2224.578 kb on + strand, within LRK53_RS09910 at 2224.578 kb on + strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.579 kb on - strand, within LRK53_RS09910 at 2224.907 kb on + strand, within LRK53_RS09910 at 2224.907 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.939 kb on + strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2224.940 kb on - strand, within LRK53_RS09910 at 2225.047 kb on + strand, within LRK53_RS09910 at 2225.047 kb on + strand, within LRK53_RS09910 at 2225.048 kb on - strand, within LRK53_RS09910 at 2225.115 kb on + strand, within LRK53_RS09910 at 2225.115 kb on + strand, within LRK53_RS09910 at 2225.115 kb on + strand, within LRK53_RS09910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,222,630 + -0.0 2,222,631 - -0.6 2,222,631 - -2.5 2,222,742 + -0.9 2,222,748 + -0.3 2,222,749 - -0.4 2,222,750 + -0.7 2,222,751 - -1.0 2,222,951 - -0.6 2,222,958 + -2.5 2,222,975 + +0.1 2,222,975 + -0.1 2,222,976 - -0.0 2,222,976 - -2.2 2,222,980 - -0.0 2,222,989 + +2.0 2,222,989 + -0.8 2,223,318 + LRK53_RS09900 0.51 +1.0 2,223,319 - LRK53_RS09900 0.52 -0.0 2,223,319 - LRK53_RS09900 0.52 -0.2 2,223,319 - LRK53_RS09900 0.52 -0.0 2,223,319 - LRK53_RS09900 0.52 +0.5 2,223,319 - LRK53_RS09900 0.52 -0.6 2,223,319 - LRK53_RS09900 0.52 -0.1 2,223,319 - LRK53_RS09900 0.52 -1.4 2,223,350 + LRK53_RS09900 0.57 -1.6 2,223,350 + LRK53_RS09900 0.57 +0.2 2,223,350 + LRK53_RS09900 0.57 -0.6 2,223,350 + LRK53_RS09900 0.57 +0.4 2,223,351 - LRK53_RS09900 0.57 +0.4 2,223,351 - LRK53_RS09900 0.57 +1.0 2,223,351 - LRK53_RS09900 0.57 -1.0 2,223,497 + LRK53_RS09900 0.80 -1.1 2,223,497 + LRK53_RS09900 0.80 -0.0 2,223,498 - LRK53_RS09900 0.80 +0.4 2,223,593 + -0.0 2,223,593 + -0.6 2,223,593 + -0.0 2,223,593 + -0.8 2,223,593 + -2.4 2,223,593 + +1.0 2,223,593 + -0.4 2,223,593 + -1.7 2,223,593 + -4.5 2,223,593 + +2.0 2,223,593 + +2.0 2,223,593 + -0.1 2,223,594 - -0.6 2,223,594 - -0.5 2,223,594 - +0.6 2,223,594 - -1.6 2,223,594 - -0.0 2,223,594 - -0.0 2,223,594 - -1.6 2,223,594 - -0.0 2,223,877 + LRK53_RS09905 0.41 -1.1 2,223,877 + LRK53_RS09905 0.41 +1.0 2,223,877 + LRK53_RS09905 0.41 -1.4 2,223,878 - LRK53_RS09905 0.42 -1.4 2,223,878 - LRK53_RS09905 0.42 -1.0 2,224,422 + LRK53_RS09910 0.12 -1.5 2,224,578 + LRK53_RS09910 0.23 +0.4 2,224,578 + LRK53_RS09910 0.23 -0.4 2,224,578 + LRK53_RS09910 0.23 +0.2 2,224,578 + LRK53_RS09910 0.23 -1.6 2,224,579 - LRK53_RS09910 0.23 -2.2 2,224,579 - LRK53_RS09910 0.23 -1.0 2,224,579 - LRK53_RS09910 0.23 +1.0 2,224,579 - LRK53_RS09910 0.23 -0.8 2,224,579 - LRK53_RS09910 0.23 -0.0 2,224,579 - LRK53_RS09910 0.23 +2.0 2,224,579 - LRK53_RS09910 0.23 +1.0 2,224,579 - LRK53_RS09910 0.23 -0.9 2,224,579 - LRK53_RS09910 0.23 -0.6 2,224,579 - LRK53_RS09910 0.23 -0.3 2,224,579 - LRK53_RS09910 0.23 -2.2 2,224,579 - LRK53_RS09910 0.23 -0.4 2,224,579 - LRK53_RS09910 0.23 -1.4 2,224,907 + LRK53_RS09910 0.46 -1.6 2,224,907 + LRK53_RS09910 0.46 -0.6 2,224,939 + LRK53_RS09910 0.49 -1.0 2,224,939 + LRK53_RS09910 0.49 +1.6 2,224,939 + LRK53_RS09910 0.49 +1.6 2,224,939 + LRK53_RS09910 0.49 +1.4 2,224,939 + LRK53_RS09910 0.49 -0.8 2,224,939 + LRK53_RS09910 0.49 -1.4 2,224,939 + LRK53_RS09910 0.49 -1.4 2,224,939 + LRK53_RS09910 0.49 -0.6 2,224,940 - LRK53_RS09910 0.49 +1.0 2,224,940 - LRK53_RS09910 0.49 -2.7 2,224,940 - LRK53_RS09910 0.49 -0.3 2,224,940 - LRK53_RS09910 0.49 +2.0 2,224,940 - LRK53_RS09910 0.49 -1.4 2,224,940 - LRK53_RS09910 0.49 +0.4 2,224,940 - LRK53_RS09910 0.49 +1.0 2,225,047 + LRK53_RS09910 0.56 -1.9 2,225,047 + LRK53_RS09910 0.56 +1.0 2,225,048 - LRK53_RS09910 0.56 -0.6 2,225,115 + LRK53_RS09910 0.61 +0.3 2,225,115 + LRK53_RS09910 0.61 +1.0 2,225,115 + LRK53_RS09910 0.61 -0.6
Or see this region's nucleotide sequence