Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS09520 and LRK53_RS09525 are separated by 132 nucleotides LRK53_RS09525 and LRK53_RS09530 are separated by 57 nucleotides
LRK53_RS09520: LRK53_RS09520 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 2,142,987 to 2,143,955
_RS09520
LRK53_RS09525: LRK53_RS09525 - RNA chaperone Hfq, at 2,144,088 to 2,144,384
_RS09525
LRK53_RS09530: LRK53_RS09530 - GTPase HflX, at 2,144,442 to 2,145,743
_RS09530
Position (kb)
2144
2145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2143.415 kb on + strand, within LRK53_RS09520 at 2143.416 kb on - strand, within LRK53_RS09520 at 2143.416 kb on - strand, within LRK53_RS09520 at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.909 kb on + strand at 2143.910 kb on - strand at 2143.997 kb on + strand at 2143.997 kb on + strand at 2143.998 kb on - strand at 2144.011 kb on + strand at 2144.011 kb on + strand at 2144.012 kb on - strand at 2144.012 kb on - strand at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.290 kb on + strand, within LRK53_RS09525 at 2144.293 kb on - strand, within LRK53_RS09525 at 2144.385 kb on + strand at 2144.385 kb on + strand at 2144.385 kb on + strand at 2144.386 kb on - strand at 2144.386 kb on - strand at 2144.386 kb on - strand at 2144.493 kb on - strand at 2144.493 kb on - strand at 2144.510 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.566 kb on + strand at 2144.567 kb on - strand at 2144.567 kb on - strand at 2144.610 kb on + strand, within LRK53_RS09530 at 2144.610 kb on + strand, within LRK53_RS09530 at 2144.611 kb on - strand, within LRK53_RS09530 at 2144.611 kb on - strand, within LRK53_RS09530 at 2144.611 kb on - strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.613 kb on + strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.614 kb on - strand, within LRK53_RS09530 at 2144.854 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.989 kb on + strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2144.990 kb on - strand, within LRK53_RS09530 at 2145.060 kb on + strand, within LRK53_RS09530 at 2145.061 kb on - strand, within LRK53_RS09530 at 2145.061 kb on - strand, within LRK53_RS09530 at 2145.114 kb on + strand, within LRK53_RS09530 at 2145.114 kb on + strand, within LRK53_RS09530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 2,143,415 + LRK53_RS09520 0.44 -2.3 2,143,416 - LRK53_RS09520 0.44 -1.1 2,143,416 - LRK53_RS09520 0.44 -0.6 2,143,909 + -1.1 2,143,909 + -0.5 2,143,909 + -0.3 2,143,909 + -0.6 2,143,909 + -0.8 2,143,909 + -1.0 2,143,910 - +0.4 2,143,997 + -0.6 2,143,997 + -1.5 2,143,998 - -0.0 2,144,011 + -1.3 2,144,011 + -0.6 2,144,012 - +0.5 2,144,012 - +3.3 2,144,290 + LRK53_RS09525 0.68 -0.0 2,144,290 + LRK53_RS09525 0.68 -2.1 2,144,290 + LRK53_RS09525 0.68 +1.0 2,144,290 + LRK53_RS09525 0.68 -0.0 2,144,290 + LRK53_RS09525 0.68 -1.0 2,144,293 - LRK53_RS09525 0.69 -0.0 2,144,385 + -1.0 2,144,385 + -0.6 2,144,385 + -0.0 2,144,386 - +0.6 2,144,386 - -0.6 2,144,386 - -1.2 2,144,493 - -1.3 2,144,493 - -0.0 2,144,510 + -0.1 2,144,566 + -0.0 2,144,566 + +0.7 2,144,566 + -0.4 2,144,566 + -2.0 2,144,566 + -0.2 2,144,567 - -0.3 2,144,567 - -0.4 2,144,610 + LRK53_RS09530 0.13 -0.2 2,144,610 + LRK53_RS09530 0.13 -0.6 2,144,611 - LRK53_RS09530 0.13 -0.6 2,144,611 - LRK53_RS09530 0.13 -1.5 2,144,611 - LRK53_RS09530 0.13 -0.6 2,144,613 + LRK53_RS09530 0.13 -1.0 2,144,613 + LRK53_RS09530 0.13 -0.3 2,144,613 + LRK53_RS09530 0.13 -0.6 2,144,613 + LRK53_RS09530 0.13 -0.9 2,144,613 + LRK53_RS09530 0.13 -1.5 2,144,613 + LRK53_RS09530 0.13 -1.6 2,144,613 + LRK53_RS09530 0.13 -0.3 2,144,613 + LRK53_RS09530 0.13 -0.6 2,144,613 + LRK53_RS09530 0.13 -0.0 2,144,613 + LRK53_RS09530 0.13 -1.8 2,144,613 + LRK53_RS09530 0.13 -0.0 2,144,613 + LRK53_RS09530 0.13 -1.2 2,144,613 + LRK53_RS09530 0.13 -0.0 2,144,613 + LRK53_RS09530 0.13 -1.0 2,144,613 + LRK53_RS09530 0.13 +0.4 2,144,613 + LRK53_RS09530 0.13 -1.6 2,144,613 + LRK53_RS09530 0.13 +0.1 2,144,613 + LRK53_RS09530 0.13 -0.0 2,144,613 + LRK53_RS09530 0.13 -1.0 2,144,613 + LRK53_RS09530 0.13 +0.7 2,144,613 + LRK53_RS09530 0.13 -1.3 2,144,613 + LRK53_RS09530 0.13 -1.3 2,144,613 + LRK53_RS09530 0.13 +0.6 2,144,613 + LRK53_RS09530 0.13 +0.5 2,144,613 + LRK53_RS09530 0.13 -0.0 2,144,614 - LRK53_RS09530 0.13 -0.2 2,144,614 - LRK53_RS09530 0.13 -0.0 2,144,614 - LRK53_RS09530 0.13 -1.0 2,144,614 - LRK53_RS09530 0.13 -2.0 2,144,614 - LRK53_RS09530 0.13 +2.0 2,144,614 - LRK53_RS09530 0.13 -0.6 2,144,614 - LRK53_RS09530 0.13 +0.6 2,144,614 - LRK53_RS09530 0.13 +0.4 2,144,614 - LRK53_RS09530 0.13 -0.0 2,144,614 - LRK53_RS09530 0.13 +1.0 2,144,614 - LRK53_RS09530 0.13 -0.8 2,144,614 - LRK53_RS09530 0.13 -0.3 2,144,614 - LRK53_RS09530 0.13 +0.2 2,144,854 + LRK53_RS09530 0.32 -1.2 2,144,989 + LRK53_RS09530 0.42 -0.6 2,144,989 + LRK53_RS09530 0.42 -0.6 2,144,989 + LRK53_RS09530 0.42 +1.0 2,144,989 + LRK53_RS09530 0.42 +0.7 2,144,989 + LRK53_RS09530 0.42 -0.6 2,144,989 + LRK53_RS09530 0.42 +0.1 2,144,989 + LRK53_RS09530 0.42 +1.6 2,144,989 + LRK53_RS09530 0.42 +0.4 2,144,989 + LRK53_RS09530 0.42 -1.0 2,144,989 + LRK53_RS09530 0.42 +0.6 2,144,989 + LRK53_RS09530 0.42 -0.4 2,144,989 + LRK53_RS09530 0.42 +0.2 2,144,989 + LRK53_RS09530 0.42 -1.0 2,144,990 - LRK53_RS09530 0.42 -2.8 2,144,990 - LRK53_RS09530 0.42 +0.2 2,144,990 - LRK53_RS09530 0.42 -1.6 2,144,990 - LRK53_RS09530 0.42 -1.0 2,144,990 - LRK53_RS09530 0.42 +0.2 2,144,990 - LRK53_RS09530 0.42 -0.0 2,145,060 + LRK53_RS09530 0.47 -1.8 2,145,061 - LRK53_RS09530 0.48 -0.6 2,145,061 - LRK53_RS09530 0.48 -0.9 2,145,114 + LRK53_RS09530 0.52 +0.1 2,145,114 + LRK53_RS09530 0.52 -1.6
Or see this region's nucleotide sequence