Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08765 and LRK53_RS08770 are separated by 115 nucleotides LRK53_RS08770 and LRK53_RS08775 are separated by 134 nucleotides LRK53_RS08775 and LRK53_RS08780 are separated by 28 nucleotides LRK53_RS08780 and LRK53_RS08785 are separated by 105 nucleotides
LRK53_RS08765: LRK53_RS08765 - histidine--tRNA ligase, at 1,972,150 to 1,973,523
_RS08765
LRK53_RS08770: LRK53_RS08770 - GIY-YIG nuclease family protein, at 1,973,639 to 1,973,932
_RS08770
LRK53_RS08775: LRK53_RS08775 - aromatic amino acid ammonia-lyase, at 1,974,067 to 1,975,956
_RS08775
LRK53_RS08780: LRK53_RS08780 - DUF6165 family protein, at 1,975,985 to 1,976,383
_RS08780
LRK53_RS08785: LRK53_RS08785 - glycosyltransferase family 9 protein, at 1,976,489 to 1,977,532
_RS08785
Position (kb) 1974
1975
1976 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.534 kb on + strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.535 kb on - strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.663 kb on + strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.664 kb on - strand at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.696 kb on + strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.697 kb on - strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.764 kb on + strand, within LRK53_RS08770 at 1973.765 kb on - strand, within LRK53_RS08770 at 1973.774 kb on + strand, within LRK53_RS08770 at 1973.930 kb on + strand at 1973.931 kb on - strand at 1973.931 kb on - strand at 1974.064 kb on + strand at 1974.064 kb on + strand at 1974.065 kb on - strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.229 kb on + strand at 1974.230 kb on - strand at 1974.230 kb on - strand at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.371 kb on + strand, within LRK53_RS08775 at 1974.372 kb on - strand, within LRK53_RS08775 at 1974.372 kb on - strand, within LRK53_RS08775 at 1974.468 kb on + strand, within LRK53_RS08775 at 1974.469 kb on - strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.607 kb on + strand, within LRK53_RS08775 at 1974.608 kb on - strand, within LRK53_RS08775 at 1974.608 kb on - strand, within LRK53_RS08775 at 1974.608 kb on - strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.884 kb on + strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.885 kb on - strand, within LRK53_RS08775 at 1974.925 kb on + strand, within LRK53_RS08775 at 1974.925 kb on + strand, within LRK53_RS08775 at 1974.926 kb on - strand, within LRK53_RS08775 at 1974.926 kb on - strand, within LRK53_RS08775 at 1975.054 kb on + strand, within LRK53_RS08775 at 1975.054 kb on + strand, within LRK53_RS08775 at 1975.055 kb on - strand, within LRK53_RS08775 at 1975.055 kb on - strand, within LRK53_RS08775 at 1975.055 kb on - strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.108 kb on + strand, within LRK53_RS08775 at 1975.109 kb on - strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.309 kb on + strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.310 kb on - strand, within LRK53_RS08775 at 1975.453 kb on + strand, within LRK53_RS08775 at 1975.453 kb on + strand, within LRK53_RS08775 at 1975.454 kb on - strand, within LRK53_RS08775 at 1975.510 kb on + strand, within LRK53_RS08775 at 1975.510 kb on + strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.511 kb on - strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.624 kb on + strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.625 kb on - strand, within LRK53_RS08775 at 1975.626 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.753 kb on + strand, within LRK53_RS08775 at 1975.754 kb on - strand, within LRK53_RS08775 at 1975.754 kb on - strand, within LRK53_RS08775 at 1975.754 kb on - strand, within LRK53_RS08775 at 1976.395 kb on - strand at 1976.490 kb on + strand at 1976.490 kb on + strand at 1976.490 kb on + strand at 1976.490 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,973,534 + -0.6 1,973,534 + +0.4 1,973,534 + +2.2 1,973,534 + -1.6 1,973,534 + -0.9 1,973,535 - +1.0 1,973,535 - -0.0 1,973,535 - -1.6 1,973,535 - -1.0 1,973,535 - -1.1 1,973,663 + -1.0 1,973,663 + -0.3 1,973,663 + +1.0 1,973,663 + -0.5 1,973,663 + -0.6 1,973,664 - -1.3 1,973,664 - +2.0 1,973,664 - -3.5 1,973,664 - -0.3 1,973,664 - -1.0 1,973,696 + LRK53_RS08770 0.19 -0.0 1,973,696 + LRK53_RS08770 0.19 -0.8 1,973,696 + LRK53_RS08770 0.19 -1.3 1,973,696 + LRK53_RS08770 0.19 -1.5 1,973,696 + LRK53_RS08770 0.19 +0.5 1,973,696 + LRK53_RS08770 0.19 +1.0 1,973,696 + LRK53_RS08770 0.19 -0.3 1,973,696 + LRK53_RS08770 0.19 -0.8 1,973,696 + LRK53_RS08770 0.19 -1.9 1,973,696 + LRK53_RS08770 0.19 -0.4 1,973,696 + LRK53_RS08770 0.19 +1.0 1,973,696 + LRK53_RS08770 0.19 -0.5 1,973,696 + LRK53_RS08770 0.19 +1.0 1,973,696 + LRK53_RS08770 0.19 -1.0 1,973,696 + LRK53_RS08770 0.19 -0.2 1,973,697 - LRK53_RS08770 0.20 -0.6 1,973,697 - LRK53_RS08770 0.20 -0.6 1,973,697 - LRK53_RS08770 0.20 -0.3 1,973,697 - LRK53_RS08770 0.20 +0.4 1,973,697 - LRK53_RS08770 0.20 -0.6 1,973,697 - LRK53_RS08770 0.20 -0.3 1,973,697 - LRK53_RS08770 0.20 -0.6 1,973,697 - LRK53_RS08770 0.20 -0.0 1,973,697 - LRK53_RS08770 0.20 +1.0 1,973,697 - LRK53_RS08770 0.20 -0.6 1,973,697 - LRK53_RS08770 0.20 -0.0 1,973,697 - LRK53_RS08770 0.20 -2.0 1,973,764 + LRK53_RS08770 0.43 -2.2 1,973,764 + LRK53_RS08770 0.43 -0.3 1,973,764 + LRK53_RS08770 0.43 +1.0 1,973,764 + LRK53_RS08770 0.43 -0.3 1,973,764 + LRK53_RS08770 0.43 -1.8 1,973,764 + LRK53_RS08770 0.43 -0.6 1,973,765 - LRK53_RS08770 0.43 -0.0 1,973,774 + LRK53_RS08770 0.46 -0.0 1,973,930 + -1.7 1,973,931 - -0.6 1,973,931 - -1.8 1,974,064 + -0.6 1,974,064 + +0.5 1,974,065 - -1.3 1,974,229 + -0.0 1,974,229 + -0.0 1,974,229 + -1.0 1,974,229 + -0.0 1,974,229 + -0.7 1,974,229 + -0.4 1,974,230 - +1.0 1,974,230 - -0.6 1,974,371 + LRK53_RS08775 0.16 -1.0 1,974,371 + LRK53_RS08775 0.16 -0.0 1,974,371 + LRK53_RS08775 0.16 -1.0 1,974,371 + LRK53_RS08775 0.16 -2.2 1,974,372 - LRK53_RS08775 0.16 +0.4 1,974,372 - LRK53_RS08775 0.16 -1.2 1,974,468 + LRK53_RS08775 0.21 -1.6 1,974,469 - LRK53_RS08775 0.21 -0.6 1,974,607 + LRK53_RS08775 0.29 -0.9 1,974,607 + LRK53_RS08775 0.29 -1.6 1,974,607 + LRK53_RS08775 0.29 -1.0 1,974,607 + LRK53_RS08775 0.29 -0.2 1,974,607 + LRK53_RS08775 0.29 -0.0 1,974,607 + LRK53_RS08775 0.29 +0.6 1,974,607 + LRK53_RS08775 0.29 -0.0 1,974,607 + LRK53_RS08775 0.29 +1.4 1,974,608 - LRK53_RS08775 0.29 -0.0 1,974,608 - LRK53_RS08775 0.29 -0.6 1,974,608 - LRK53_RS08775 0.29 -0.6 1,974,884 + LRK53_RS08775 0.43 -0.6 1,974,884 + LRK53_RS08775 0.43 +0.4 1,974,884 + LRK53_RS08775 0.43 +2.0 1,974,884 + LRK53_RS08775 0.43 +1.0 1,974,884 + LRK53_RS08775 0.43 -0.0 1,974,884 + LRK53_RS08775 0.43 -0.0 1,974,884 + LRK53_RS08775 0.43 -0.2 1,974,884 + LRK53_RS08775 0.43 -1.2 1,974,884 + LRK53_RS08775 0.43 +1.0 1,974,884 + LRK53_RS08775 0.43 -0.6 1,974,884 + LRK53_RS08775 0.43 -1.3 1,974,885 - LRK53_RS08775 0.43 +1.0 1,974,885 - LRK53_RS08775 0.43 -0.8 1,974,885 - LRK53_RS08775 0.43 -0.6 1,974,885 - LRK53_RS08775 0.43 +1.0 1,974,885 - LRK53_RS08775 0.43 -1.0 1,974,885 - LRK53_RS08775 0.43 -1.0 1,974,885 - LRK53_RS08775 0.43 -0.8 1,974,885 - LRK53_RS08775 0.43 -2.0 1,974,885 - LRK53_RS08775 0.43 +1.0 1,974,885 - LRK53_RS08775 0.43 -0.6 1,974,925 + LRK53_RS08775 0.45 -0.3 1,974,925 + LRK53_RS08775 0.45 +1.0 1,974,926 - LRK53_RS08775 0.45 +1.0 1,974,926 - LRK53_RS08775 0.45 -0.6 1,975,054 + LRK53_RS08775 0.52 +0.4 1,975,054 + LRK53_RS08775 0.52 -0.0 1,975,055 - LRK53_RS08775 0.52 +2.0 1,975,055 - LRK53_RS08775 0.52 +0.3 1,975,055 - LRK53_RS08775 0.52 -0.6 1,975,108 + LRK53_RS08775 0.55 -0.0 1,975,108 + LRK53_RS08775 0.55 -0.3 1,975,108 + LRK53_RS08775 0.55 -0.3 1,975,108 + LRK53_RS08775 0.55 -0.2 1,975,108 + LRK53_RS08775 0.55 -1.3 1,975,108 + LRK53_RS08775 0.55 -0.4 1,975,109 - LRK53_RS08775 0.55 +2.0 1,975,309 + LRK53_RS08775 0.66 -1.0 1,975,309 + LRK53_RS08775 0.66 -2.2 1,975,309 + LRK53_RS08775 0.66 +1.0 1,975,309 + LRK53_RS08775 0.66 -0.8 1,975,309 + LRK53_RS08775 0.66 -1.1 1,975,310 - LRK53_RS08775 0.66 -0.6 1,975,310 - LRK53_RS08775 0.66 -1.8 1,975,310 - LRK53_RS08775 0.66 -0.0 1,975,310 - LRK53_RS08775 0.66 -1.9 1,975,310 - LRK53_RS08775 0.66 -0.6 1,975,453 + LRK53_RS08775 0.73 -1.0 1,975,453 + LRK53_RS08775 0.73 -0.0 1,975,454 - LRK53_RS08775 0.73 +0.4 1,975,510 + LRK53_RS08775 0.76 -0.6 1,975,510 + LRK53_RS08775 0.76 -2.2 1,975,511 - LRK53_RS08775 0.76 -0.0 1,975,511 - LRK53_RS08775 0.76 -0.0 1,975,511 - LRK53_RS08775 0.76 +1.3 1,975,511 - LRK53_RS08775 0.76 -1.5 1,975,511 - LRK53_RS08775 0.76 -0.0 1,975,511 - LRK53_RS08775 0.76 -2.0 1,975,624 + LRK53_RS08775 0.82 -2.0 1,975,624 + LRK53_RS08775 0.82 -1.8 1,975,624 + LRK53_RS08775 0.82 -2.7 1,975,624 + LRK53_RS08775 0.82 -0.0 1,975,624 + LRK53_RS08775 0.82 -1.5 1,975,624 + LRK53_RS08775 0.82 -1.0 1,975,624 + LRK53_RS08775 0.82 -0.6 1,975,624 + LRK53_RS08775 0.82 -0.0 1,975,624 + LRK53_RS08775 0.82 +1.0 1,975,624 + LRK53_RS08775 0.82 -0.0 1,975,624 + LRK53_RS08775 0.82 -1.2 1,975,624 + LRK53_RS08775 0.82 -0.0 1,975,625 - LRK53_RS08775 0.82 +1.0 1,975,625 - LRK53_RS08775 0.82 -2.3 1,975,625 - LRK53_RS08775 0.82 -0.6 1,975,625 - LRK53_RS08775 0.82 -1.0 1,975,625 - LRK53_RS08775 0.82 -1.3 1,975,625 - LRK53_RS08775 0.82 -2.5 1,975,625 - LRK53_RS08775 0.82 -0.0 1,975,625 - LRK53_RS08775 0.82 -1.2 1,975,625 - LRK53_RS08775 0.82 -1.6 1,975,625 - LRK53_RS08775 0.82 +1.0 1,975,626 + LRK53_RS08775 0.82 -1.0 1,975,753 + LRK53_RS08775 0.89 -0.0 1,975,753 + LRK53_RS08775 0.89 -1.3 1,975,753 + LRK53_RS08775 0.89 -0.6 1,975,753 + LRK53_RS08775 0.89 -0.9 1,975,754 - LRK53_RS08775 0.89 +0.1 1,975,754 - LRK53_RS08775 0.89 -1.0 1,975,754 - LRK53_RS08775 0.89 +1.0 1,976,395 - +1.0 1,976,490 + +1.0 1,976,490 + -0.0 1,976,490 + +1.0 1,976,490 + -0.6
Or see this region's nucleotide sequence