Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08725 and LRK53_RS08730 overlap by 8 nucleotides LRK53_RS08730 and LRK53_RS08735 are separated by 260 nucleotides LRK53_RS08735 and LRK53_RS08740 are separated by 26 nucleotides LRK53_RS08740 and LRK53_RS08745 are separated by 71 nucleotides
LRK53_RS08725: LRK53_RS08725 - TonB-dependent receptor, at 1,961,960 to 1,964,194
_RS08725
LRK53_RS08730: LRK53_RS08730 - hypothetical protein, at 1,964,187 to 1,964,531
_RS08730
LRK53_RS08735: LRK53_RS08735 - Xaa-Pro peptidase family protein, at 1,964,792 to 1,965,985
_RS08735
LRK53_RS08740: LRK53_RS08740 - hypothetical protein, at 1,966,012 to 1,966,434
_RS08740
LRK53_RS08745: LRK53_RS08745 - LuxR C-terminal-related transcriptional regulator, at 1,966,506 to 1,969,205
_RS08745
Position (kb) 1964
1965
1966 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1964.045 kb on + strand at 1964.045 kb on + strand at 1964.046 kb on - strand at 1964.046 kb on - strand at 1964.046 kb on - strand at 1964.046 kb on - strand at 1964.056 kb on + strand at 1964.056 kb on + strand at 1964.056 kb on + strand at 1964.057 kb on - strand at 1964.057 kb on - strand at 1964.057 kb on - strand at 1964.057 kb on - strand at 1964.057 kb on - strand at 1964.057 kb on - strand at 1964.057 kb on - strand at 1964.108 kb on - strand at 1964.186 kb on + strand at 1964.186 kb on + strand at 1964.186 kb on + strand at 1964.187 kb on - strand at 1964.187 kb on - strand at 1964.187 kb on - strand at 1964.187 kb on - strand at 1964.218 kb on + strand at 1964.218 kb on + strand at 1964.219 kb on - strand at 1964.219 kb on - strand at 1964.219 kb on - strand at 1964.219 kb on - strand at 1964.313 kb on - strand, within LRK53_RS08730 at 1964.313 kb on - strand, within LRK53_RS08730 at 1964.313 kb on - strand, within LRK53_RS08730 at 1964.313 kb on - strand, within LRK53_RS08730 at 1964.342 kb on - strand, within LRK53_RS08730 at 1964.618 kb on + strand at 1964.618 kb on + strand at 1964.619 kb on - strand at 1964.619 kb on - strand at 1964.619 kb on - strand at 1964.681 kb on + strand at 1964.682 kb on - strand at 1964.729 kb on + strand at 1964.811 kb on + strand at 1964.811 kb on + strand at 1964.811 kb on + strand at 1964.811 kb on + strand at 1964.812 kb on - strand at 1964.812 kb on - strand at 1964.812 kb on - strand at 1964.812 kb on - strand at 1964.812 kb on - strand at 1964.812 kb on - strand at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.963 kb on + strand, within LRK53_RS08735 at 1964.964 kb on - strand, within LRK53_RS08735 at 1964.964 kb on - strand, within LRK53_RS08735 at 1964.964 kb on - strand, within LRK53_RS08735 at 1964.964 kb on - strand, within LRK53_RS08735 at 1965.035 kb on + strand, within LRK53_RS08735 at 1965.035 kb on + strand, within LRK53_RS08735 at 1965.035 kb on + strand, within LRK53_RS08735 at 1965.035 kb on + strand, within LRK53_RS08735 at 1965.036 kb on - strand, within LRK53_RS08735 at 1965.036 kb on - strand, within LRK53_RS08735 at 1965.036 kb on - strand, within LRK53_RS08735 at 1965.087 kb on + strand, within LRK53_RS08735 at 1965.087 kb on + strand, within LRK53_RS08735 at 1965.087 kb on + strand, within LRK53_RS08735 at 1965.088 kb on - strand, within LRK53_RS08735 at 1965.088 kb on - strand, within LRK53_RS08735 at 1965.088 kb on - strand, within LRK53_RS08735 at 1965.088 kb on - strand, within LRK53_RS08735 at 1965.088 kb on - strand, within LRK53_RS08735 at 1965.088 kb on - strand, within LRK53_RS08735 at 1965.150 kb on + strand, within LRK53_RS08735 at 1965.150 kb on + strand, within LRK53_RS08735 at 1965.150 kb on + strand, within LRK53_RS08735 at 1965.150 kb on + strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.151 kb on - strand, within LRK53_RS08735 at 1965.221 kb on + strand, within LRK53_RS08735 at 1965.221 kb on + strand, within LRK53_RS08735 at 1965.221 kb on + strand, within LRK53_RS08735 at 1965.222 kb on - strand, within LRK53_RS08735 at 1965.373 kb on + strand, within LRK53_RS08735 at 1965.390 kb on + strand, within LRK53_RS08735 at 1965.391 kb on - strand, within LRK53_RS08735 at 1965.557 kb on + strand, within LRK53_RS08735 at 1965.557 kb on + strand, within LRK53_RS08735 at 1965.557 kb on + strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.558 kb on - strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.581 kb on + strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.582 kb on - strand, within LRK53_RS08735 at 1965.807 kb on - strand, within LRK53_RS08735 at 1965.807 kb on - strand, within LRK53_RS08735 at 1965.807 kb on - strand, within LRK53_RS08735 at 1965.914 kb on + strand at 1965.914 kb on + strand at 1965.914 kb on + strand at 1965.914 kb on + strand at 1965.914 kb on + strand at 1965.914 kb on + strand at 1965.915 kb on - strand at 1965.915 kb on - strand at 1965.915 kb on - strand at 1965.915 kb on - strand at 1965.915 kb on - strand at 1966.076 kb on + strand, within LRK53_RS08740 at 1966.076 kb on + strand, within LRK53_RS08740 at 1966.076 kb on + strand, within LRK53_RS08740 at 1966.076 kb on + strand, within LRK53_RS08740 at 1966.076 kb on + strand, within LRK53_RS08740 at 1966.077 kb on - strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.081 kb on + strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.082 kb on - strand, within LRK53_RS08740 at 1966.182 kb on + strand, within LRK53_RS08740 at 1966.182 kb on + strand, within LRK53_RS08740 at 1966.182 kb on + strand, within LRK53_RS08740 at 1966.182 kb on + strand, within LRK53_RS08740 at 1966.183 kb on - strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.218 kb on + strand, within LRK53_RS08740 at 1966.219 kb on - strand, within LRK53_RS08740 at 1966.219 kb on - strand, within LRK53_RS08740 at 1966.219 kb on - strand, within LRK53_RS08740 at 1966.219 kb on - strand, within LRK53_RS08740 at 1966.422 kb on + strand at 1966.423 kb on - strand at 1966.423 kb on - strand at 1966.423 kb on - strand at 1966.438 kb on + strand at 1966.438 kb on + strand at 1966.438 kb on + strand at 1966.438 kb on + strand at 1966.438 kb on + strand at 1966.438 kb on + strand at 1966.463 kb on + strand at 1966.464 kb on - strand at 1966.464 kb on - strand at 1966.464 kb on - strand at 1966.588 kb on + strand at 1966.588 kb on + strand at 1966.588 kb on + strand at 1966.589 kb on - strand at 1966.589 kb on - strand at 1966.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,964,045 + -2.4 1,964,045 + -2.0 1,964,046 - +0.2 1,964,046 - -1.1 1,964,046 - -0.0 1,964,046 - -1.1 1,964,056 + -1.0 1,964,056 + -2.6 1,964,056 + +1.6 1,964,057 - -1.2 1,964,057 - +1.0 1,964,057 - +0.4 1,964,057 - +1.6 1,964,057 - -0.3 1,964,057 - +0.5 1,964,057 - -1.4 1,964,108 - -2.0 1,964,186 + -1.5 1,964,186 + -0.2 1,964,186 + -0.6 1,964,187 - -1.1 1,964,187 - -0.0 1,964,187 - -1.7 1,964,187 - -0.4 1,964,218 + -2.5 1,964,218 + +0.3 1,964,219 - -1.2 1,964,219 - -0.6 1,964,219 - +0.3 1,964,219 - -1.1 1,964,313 - LRK53_RS08730 0.37 -0.1 1,964,313 - LRK53_RS08730 0.37 -0.3 1,964,313 - LRK53_RS08730 0.37 -0.4 1,964,313 - LRK53_RS08730 0.37 +1.0 1,964,342 - LRK53_RS08730 0.45 -1.2 1,964,618 + +0.6 1,964,618 + -0.0 1,964,619 - -1.2 1,964,619 - +0.4 1,964,619 - -0.7 1,964,681 + -0.7 1,964,682 - -0.9 1,964,729 + -0.0 1,964,811 + -1.0 1,964,811 + -1.2 1,964,811 + -0.9 1,964,811 + +0.1 1,964,812 - -2.2 1,964,812 - +0.4 1,964,812 - +0.1 1,964,812 - -2.5 1,964,812 - +1.0 1,964,812 - -0.6 1,964,963 + LRK53_RS08735 0.14 -0.0 1,964,963 + LRK53_RS08735 0.14 -0.2 1,964,963 + LRK53_RS08735 0.14 -0.4 1,964,963 + LRK53_RS08735 0.14 +1.0 1,964,963 + LRK53_RS08735 0.14 -0.8 1,964,963 + LRK53_RS08735 0.14 -0.7 1,964,963 + LRK53_RS08735 0.14 -0.0 1,964,964 - LRK53_RS08735 0.14 -2.6 1,964,964 - LRK53_RS08735 0.14 -0.6 1,964,964 - LRK53_RS08735 0.14 +1.0 1,964,964 - LRK53_RS08735 0.14 -2.3 1,965,035 + LRK53_RS08735 0.20 +0.5 1,965,035 + LRK53_RS08735 0.20 -0.0 1,965,035 + LRK53_RS08735 0.20 -0.7 1,965,035 + LRK53_RS08735 0.20 -0.6 1,965,036 - LRK53_RS08735 0.20 +2.0 1,965,036 - LRK53_RS08735 0.20 -0.4 1,965,036 - LRK53_RS08735 0.20 -0.0 1,965,087 + LRK53_RS08735 0.25 -1.2 1,965,087 + LRK53_RS08735 0.25 -1.0 1,965,087 + LRK53_RS08735 0.25 -0.0 1,965,088 - LRK53_RS08735 0.25 +0.2 1,965,088 - LRK53_RS08735 0.25 -0.8 1,965,088 - LRK53_RS08735 0.25 -0.8 1,965,088 - LRK53_RS08735 0.25 -1.3 1,965,088 - LRK53_RS08735 0.25 -0.0 1,965,088 - LRK53_RS08735 0.25 -0.8 1,965,150 + LRK53_RS08735 0.30 -0.6 1,965,150 + LRK53_RS08735 0.30 -0.6 1,965,150 + LRK53_RS08735 0.30 +2.0 1,965,150 + LRK53_RS08735 0.30 -1.1 1,965,151 - LRK53_RS08735 0.30 -0.5 1,965,151 - LRK53_RS08735 0.30 +1.0 1,965,151 - LRK53_RS08735 0.30 -0.1 1,965,151 - LRK53_RS08735 0.30 -1.6 1,965,151 - LRK53_RS08735 0.30 -0.6 1,965,151 - LRK53_RS08735 0.30 -0.0 1,965,151 - LRK53_RS08735 0.30 -0.4 1,965,151 - LRK53_RS08735 0.30 +0.7 1,965,151 - LRK53_RS08735 0.30 +0.3 1,965,151 - LRK53_RS08735 0.30 -1.3 1,965,151 - LRK53_RS08735 0.30 -0.0 1,965,151 - LRK53_RS08735 0.30 -1.0 1,965,221 + LRK53_RS08735 0.36 +0.4 1,965,221 + LRK53_RS08735 0.36 -0.0 1,965,221 + LRK53_RS08735 0.36 -1.3 1,965,222 - LRK53_RS08735 0.36 -0.6 1,965,373 + LRK53_RS08735 0.49 -0.4 1,965,390 + LRK53_RS08735 0.50 -1.6 1,965,391 - LRK53_RS08735 0.50 -1.6 1,965,557 + LRK53_RS08735 0.64 +2.0 1,965,557 + LRK53_RS08735 0.64 -0.4 1,965,557 + LRK53_RS08735 0.64 -0.8 1,965,558 - LRK53_RS08735 0.64 -1.5 1,965,558 - LRK53_RS08735 0.64 -1.8 1,965,558 - LRK53_RS08735 0.64 +0.7 1,965,558 - LRK53_RS08735 0.64 -0.6 1,965,558 - LRK53_RS08735 0.64 -0.1 1,965,558 - LRK53_RS08735 0.64 -0.0 1,965,558 - LRK53_RS08735 0.64 -1.0 1,965,581 + LRK53_RS08735 0.66 +0.7 1,965,581 + LRK53_RS08735 0.66 -0.1 1,965,581 + LRK53_RS08735 0.66 -1.0 1,965,581 + LRK53_RS08735 0.66 -1.1 1,965,581 + LRK53_RS08735 0.66 +0.6 1,965,581 + LRK53_RS08735 0.66 +0.1 1,965,581 + LRK53_RS08735 0.66 -1.2 1,965,581 + LRK53_RS08735 0.66 -2.5 1,965,581 + LRK53_RS08735 0.66 -1.5 1,965,581 + LRK53_RS08735 0.66 -0.9 1,965,581 + LRK53_RS08735 0.66 -0.0 1,965,581 + LRK53_RS08735 0.66 -0.0 1,965,581 + LRK53_RS08735 0.66 -0.8 1,965,581 + LRK53_RS08735 0.66 -0.9 1,965,581 + LRK53_RS08735 0.66 +0.5 1,965,581 + LRK53_RS08735 0.66 -2.0 1,965,581 + LRK53_RS08735 0.66 +0.6 1,965,581 + LRK53_RS08735 0.66 +1.5 1,965,581 + LRK53_RS08735 0.66 +0.4 1,965,582 - LRK53_RS08735 0.66 -0.5 1,965,582 - LRK53_RS08735 0.66 +1.0 1,965,582 - LRK53_RS08735 0.66 -0.6 1,965,582 - LRK53_RS08735 0.66 +0.4 1,965,582 - LRK53_RS08735 0.66 -0.7 1,965,582 - LRK53_RS08735 0.66 -0.3 1,965,582 - LRK53_RS08735 0.66 -0.6 1,965,582 - LRK53_RS08735 0.66 +0.4 1,965,582 - LRK53_RS08735 0.66 -0.7 1,965,582 - LRK53_RS08735 0.66 -0.6 1,965,582 - LRK53_RS08735 0.66 -1.1 1,965,582 - LRK53_RS08735 0.66 -0.4 1,965,582 - LRK53_RS08735 0.66 -1.7 1,965,807 - LRK53_RS08735 0.85 -0.0 1,965,807 - LRK53_RS08735 0.85 +0.1 1,965,807 - LRK53_RS08735 0.85 +1.0 1,965,914 + -0.6 1,965,914 + -0.6 1,965,914 + -2.8 1,965,914 + -0.6 1,965,914 + -0.1 1,965,914 + +1.0 1,965,915 - -1.0 1,965,915 - -0.6 1,965,915 - -0.2 1,965,915 - -1.0 1,965,915 - -1.6 1,966,076 + LRK53_RS08740 0.15 -0.0 1,966,076 + LRK53_RS08740 0.15 +1.0 1,966,076 + LRK53_RS08740 0.15 +2.6 1,966,076 + LRK53_RS08740 0.15 -0.3 1,966,076 + LRK53_RS08740 0.15 +1.0 1,966,077 - LRK53_RS08740 0.15 +0.6 1,966,081 + LRK53_RS08740 0.16 -1.4 1,966,081 + LRK53_RS08740 0.16 +0.8 1,966,081 + LRK53_RS08740 0.16 +1.0 1,966,081 + LRK53_RS08740 0.16 -0.0 1,966,081 + LRK53_RS08740 0.16 +1.0 1,966,081 + LRK53_RS08740 0.16 +2.0 1,966,081 + LRK53_RS08740 0.16 +0.4 1,966,081 + LRK53_RS08740 0.16 -0.9 1,966,081 + LRK53_RS08740 0.16 -1.0 1,966,082 - LRK53_RS08740 0.17 -0.6 1,966,082 - LRK53_RS08740 0.17 +1.0 1,966,082 - LRK53_RS08740 0.17 -0.8 1,966,082 - LRK53_RS08740 0.17 +1.0 1,966,082 - LRK53_RS08740 0.17 -0.7 1,966,082 - LRK53_RS08740 0.17 -0.0 1,966,082 - LRK53_RS08740 0.17 +0.3 1,966,082 - LRK53_RS08740 0.17 -0.3 1,966,082 - LRK53_RS08740 0.17 -0.0 1,966,182 + LRK53_RS08740 0.40 +1.0 1,966,182 + LRK53_RS08740 0.40 +1.0 1,966,182 + LRK53_RS08740 0.40 -2.3 1,966,182 + LRK53_RS08740 0.40 -0.3 1,966,183 - LRK53_RS08740 0.40 -1.3 1,966,218 + LRK53_RS08740 0.49 -1.3 1,966,218 + LRK53_RS08740 0.49 -0.6 1,966,218 + LRK53_RS08740 0.49 +1.0 1,966,218 + LRK53_RS08740 0.49 -2.5 1,966,218 + LRK53_RS08740 0.49 -0.0 1,966,218 + LRK53_RS08740 0.49 -1.3 1,966,218 + LRK53_RS08740 0.49 -0.3 1,966,218 + LRK53_RS08740 0.49 +0.3 1,966,218 + LRK53_RS08740 0.49 -0.6 1,966,218 + LRK53_RS08740 0.49 -1.0 1,966,218 + LRK53_RS08740 0.49 -0.0 1,966,219 - LRK53_RS08740 0.49 -1.2 1,966,219 - LRK53_RS08740 0.49 -1.9 1,966,219 - LRK53_RS08740 0.49 -1.0 1,966,219 - LRK53_RS08740 0.49 -1.5 1,966,422 + -0.4 1,966,423 - -1.0 1,966,423 - +2.0 1,966,423 - -0.5 1,966,438 + -0.2 1,966,438 + -3.0 1,966,438 + -0.4 1,966,438 + -1.0 1,966,438 + -0.6 1,966,438 + -1.4 1,966,463 + -2.0 1,966,464 - +1.0 1,966,464 - -1.1 1,966,464 - -1.4 1,966,588 + -0.6 1,966,588 + -0.5 1,966,588 + +1.0 1,966,589 - -1.7 1,966,589 - -0.5 1,966,589 - -0.0
Or see this region's nucleotide sequence