Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08405 and LRK53_RS08410 are separated by 100 nucleotides LRK53_RS08410 and LRK53_RS08415 are separated by 5 nucleotides LRK53_RS08415 and LRK53_RS08420 are separated by 97 nucleotides LRK53_RS08420 and LRK53_RS08425 are separated by 58 nucleotides
LRK53_RS08405: LRK53_RS08405 - response regulator transcription factor, at 1,891,119 to 1,891,796
_RS08405
LRK53_RS08410: LRK53_RS08410 - hypothetical protein, at 1,891,897 to 1,892,202
_RS08410
LRK53_RS08415: LRK53_RS08415 - transcriptional repressor, at 1,892,208 to 1,892,696
_RS08415
LRK53_RS08420: LRK53_RS08420 - MerC domain-containing protein, at 1,892,794 to 1,893,228
_RS08420
LRK53_RS08425: LRK53_RS08425 - cation diffusion facilitator family transporter, at 1,893,287 to 1,894,231
_RS08425
Position (kb)
1892
1893
1894 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1891.871 kb on + strand at 1891.872 kb on - strand at 1891.877 kb on - strand at 1891.877 kb on - strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.183 kb on + strand at 1892.184 kb on - strand at 1892.184 kb on - strand at 1892.382 kb on + strand, within LRK53_RS08415 at 1892.382 kb on + strand, within LRK53_RS08415 at 1892.382 kb on + strand, within LRK53_RS08415 at 1892.382 kb on + strand, within LRK53_RS08415 at 1892.383 kb on - strand, within LRK53_RS08415 at 1892.383 kb on - strand, within LRK53_RS08415 at 1892.439 kb on + strand, within LRK53_RS08415 at 1892.439 kb on + strand, within LRK53_RS08415 at 1892.450 kb on - strand, within LRK53_RS08415 at 1892.450 kb on - strand, within LRK53_RS08415 at 1892.450 kb on - strand, within LRK53_RS08415 at 1892.558 kb on + strand, within LRK53_RS08415 at 1892.558 kb on + strand, within LRK53_RS08415 at 1892.558 kb on + strand, within LRK53_RS08415 at 1892.558 kb on + strand, within LRK53_RS08415 at 1892.730 kb on + strand at 1892.730 kb on + strand at 1892.731 kb on - strand at 1892.731 kb on - strand at 1892.731 kb on - strand at 1892.731 kb on - strand at 1892.731 kb on - strand at 1892.732 kb on + strand at 1892.732 kb on + strand at 1892.732 kb on + strand at 1892.732 kb on + strand at 1892.732 kb on + strand at 1892.732 kb on + strand at 1892.733 kb on - strand at 1892.733 kb on - strand at 1892.737 kb on + strand at 1892.737 kb on + strand at 1892.738 kb on - strand at 1892.738 kb on - strand at 1892.738 kb on - strand at 1892.738 kb on - strand at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.085 kb on + strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.086 kb on - strand, within LRK53_RS08420 at 1893.088 kb on - strand, within LRK53_RS08420 at 1893.088 kb on - strand, within LRK53_RS08420 at 1893.088 kb on - strand, within LRK53_RS08420 at 1893.275 kb on + strand at 1893.275 kb on + strand at 1893.275 kb on + strand at 1893.275 kb on + strand at 1893.275 kb on + strand at 1893.275 kb on + strand at 1893.276 kb on - strand at 1893.276 kb on - strand at 1893.276 kb on - strand at 1893.276 kb on - strand at 1893.387 kb on + strand, within LRK53_RS08425 at 1893.548 kb on + strand, within LRK53_RS08425 at 1893.548 kb on + strand, within LRK53_RS08425 at 1893.548 kb on + strand, within LRK53_RS08425 at 1893.549 kb on - strand, within LRK53_RS08425 at 1893.549 kb on - strand, within LRK53_RS08425 at 1893.549 kb on - strand, within LRK53_RS08425 at 1893.549 kb on - strand, within LRK53_RS08425 at 1893.549 kb on - strand, within LRK53_RS08425 at 1893.554 kb on + strand, within LRK53_RS08425 at 1893.626 kb on + strand, within LRK53_RS08425 at 1893.626 kb on + strand, within LRK53_RS08425 at 1893.727 kb on + strand, within LRK53_RS08425 at 1893.728 kb on - strand, within LRK53_RS08425 at 1893.728 kb on - strand, within LRK53_RS08425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,891,871 + -0.6 1,891,872 - +0.6 1,891,877 - -0.6 1,891,877 - +0.6 1,892,183 + -0.6 1,892,183 + +0.6 1,892,183 + +1.0 1,892,183 + -0.0 1,892,183 + +0.6 1,892,183 + +0.7 1,892,183 + -0.1 1,892,183 + -1.0 1,892,184 - -1.0 1,892,184 - -1.1 1,892,382 + LRK53_RS08415 0.36 -0.3 1,892,382 + LRK53_RS08415 0.36 +2.0 1,892,382 + LRK53_RS08415 0.36 +0.4 1,892,382 + LRK53_RS08415 0.36 -0.0 1,892,383 - LRK53_RS08415 0.36 -1.0 1,892,383 - LRK53_RS08415 0.36 +0.2 1,892,439 + LRK53_RS08415 0.47 +1.0 1,892,439 + LRK53_RS08415 0.47 -0.7 1,892,450 - LRK53_RS08415 0.49 -1.0 1,892,450 - LRK53_RS08415 0.49 -1.3 1,892,450 - LRK53_RS08415 0.49 -0.1 1,892,558 + LRK53_RS08415 0.72 -0.6 1,892,558 + LRK53_RS08415 0.72 +0.4 1,892,558 + LRK53_RS08415 0.72 -0.0 1,892,558 + LRK53_RS08415 0.72 -0.7 1,892,730 + -1.8 1,892,730 + +1.0 1,892,731 - -0.0 1,892,731 - -1.6 1,892,731 - -1.0 1,892,731 - -0.6 1,892,731 - -0.6 1,892,732 + -2.3 1,892,732 + -0.1 1,892,732 + -0.2 1,892,732 + -1.5 1,892,732 + -0.0 1,892,732 + +0.4 1,892,733 - -1.3 1,892,733 - -0.4 1,892,737 + -1.3 1,892,737 + -0.8 1,892,738 - -1.3 1,892,738 - -0.0 1,892,738 - -0.0 1,892,738 - +0.3 1,893,085 + LRK53_RS08420 0.67 -0.6 1,893,085 + LRK53_RS08420 0.67 +1.0 1,893,085 + LRK53_RS08420 0.67 -0.0 1,893,085 + LRK53_RS08420 0.67 -0.6 1,893,085 + LRK53_RS08420 0.67 +0.3 1,893,085 + LRK53_RS08420 0.67 +0.6 1,893,085 + LRK53_RS08420 0.67 -0.0 1,893,085 + LRK53_RS08420 0.67 -0.0 1,893,085 + LRK53_RS08420 0.67 -1.2 1,893,085 + LRK53_RS08420 0.67 -0.8 1,893,086 - LRK53_RS08420 0.67 -1.3 1,893,086 - LRK53_RS08420 0.67 +1.0 1,893,086 - LRK53_RS08420 0.67 +0.4 1,893,086 - LRK53_RS08420 0.67 -1.0 1,893,086 - LRK53_RS08420 0.67 -1.0 1,893,086 - LRK53_RS08420 0.67 -0.0 1,893,086 - LRK53_RS08420 0.67 -1.8 1,893,086 - LRK53_RS08420 0.67 -3.0 1,893,086 - LRK53_RS08420 0.67 +1.0 1,893,086 - LRK53_RS08420 0.67 -1.0 1,893,086 - LRK53_RS08420 0.67 -0.8 1,893,086 - LRK53_RS08420 0.67 -0.8 1,893,086 - LRK53_RS08420 0.67 -0.9 1,893,086 - LRK53_RS08420 0.67 -2.6 1,893,086 - LRK53_RS08420 0.67 -1.8 1,893,086 - LRK53_RS08420 0.67 -0.6 1,893,086 - LRK53_RS08420 0.67 -0.2 1,893,086 - LRK53_RS08420 0.67 -0.0 1,893,086 - LRK53_RS08420 0.67 +1.0 1,893,086 - LRK53_RS08420 0.67 -0.2 1,893,088 - LRK53_RS08420 0.68 -0.0 1,893,088 - LRK53_RS08420 0.68 -1.6 1,893,088 - LRK53_RS08420 0.68 -0.0 1,893,275 + -0.1 1,893,275 + -0.6 1,893,275 + -0.0 1,893,275 + -2.2 1,893,275 + -1.6 1,893,275 + +0.4 1,893,276 - +1.3 1,893,276 - +0.5 1,893,276 - -0.1 1,893,276 - -0.6 1,893,387 + LRK53_RS08425 0.11 +0.5 1,893,548 + LRK53_RS08425 0.28 -0.6 1,893,548 + LRK53_RS08425 0.28 -1.3 1,893,548 + LRK53_RS08425 0.28 +1.0 1,893,549 - LRK53_RS08425 0.28 -0.7 1,893,549 - LRK53_RS08425 0.28 +0.7 1,893,549 - LRK53_RS08425 0.28 -1.3 1,893,549 - LRK53_RS08425 0.28 -2.1 1,893,549 - LRK53_RS08425 0.28 +0.6 1,893,554 + LRK53_RS08425 0.28 -2.3 1,893,626 + LRK53_RS08425 0.36 -0.0 1,893,626 + LRK53_RS08425 0.36 +0.4 1,893,727 + LRK53_RS08425 0.47 -1.0 1,893,728 - LRK53_RS08425 0.47 -2.0 1,893,728 - LRK53_RS08425 0.47 +0.4
Or see this region's nucleotide sequence