Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS08135 and LRK53_RS08140 are separated by 217 nucleotides LRK53_RS08140 and LRK53_RS08145 are separated by 66 nucleotides
LRK53_RS08135: LRK53_RS08135 - PepSY domain-containing protein, at 1,827,749 to 1,829,581
_RS08135
LRK53_RS08140: LRK53_RS08140 - hypothetical protein, at 1,829,799 to 1,830,188
_RS08140
LRK53_RS08145: LRK53_RS08145 - TolC family protein, at 1,830,255 to 1,831,502
_RS08145
Position (kb) 1829
1830
1831 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1828.809 kb on + strand, within LRK53_RS08135 at 1828.809 kb on + strand, within LRK53_RS08135 at 1828.809 kb on + strand, within LRK53_RS08135 at 1828.810 kb on - strand, within LRK53_RS08135 at 1828.810 kb on - strand, within LRK53_RS08135 at 1828.810 kb on - strand, within LRK53_RS08135 at 1828.810 kb on - strand, within LRK53_RS08135 at 1828.859 kb on + strand, within LRK53_RS08135 at 1828.859 kb on + strand, within LRK53_RS08135 at 1828.859 kb on + strand, within LRK53_RS08135 at 1828.859 kb on + strand, within LRK53_RS08135 at 1828.859 kb on + strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.860 kb on - strand, within LRK53_RS08135 at 1828.861 kb on - strand, within LRK53_RS08135 at 1828.883 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.903 kb on + strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.904 kb on - strand, within LRK53_RS08135 at 1828.991 kb on + strand, within LRK53_RS08135 at 1828.991 kb on + strand, within LRK53_RS08135 at 1828.992 kb on - strand, within LRK53_RS08135 at 1828.992 kb on - strand, within LRK53_RS08135 at 1829.102 kb on + strand, within LRK53_RS08135 at 1829.103 kb on - strand, within LRK53_RS08135 at 1829.103 kb on - strand, within LRK53_RS08135 at 1829.103 kb on - strand, within LRK53_RS08135 at 1829.103 kb on - strand, within LRK53_RS08135 at 1829.108 kb on + strand, within LRK53_RS08135 at 1829.109 kb on - strand, within LRK53_RS08135 at 1829.194 kb on + strand, within LRK53_RS08135 at 1829.283 kb on - strand, within LRK53_RS08135 at 1829.283 kb on - strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.312 kb on + strand, within LRK53_RS08135 at 1829.313 kb on - strand, within LRK53_RS08135 at 1829.313 kb on - strand, within LRK53_RS08135 at 1829.313 kb on - strand, within LRK53_RS08135 at 1829.480 kb on + strand at 1829.481 kb on - strand at 1829.556 kb on + strand at 1829.557 kb on - strand at 1829.557 kb on - strand at 1829.575 kb on + strand at 1829.577 kb on + strand at 1829.577 kb on + strand at 1829.577 kb on + strand at 1829.577 kb on + strand at 1829.577 kb on + strand at 1829.577 kb on + strand at 1829.577 kb on + strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.578 kb on - strand at 1829.786 kb on + strand at 1829.787 kb on - strand at 1829.787 kb on - strand at 1829.787 kb on - strand at 1829.787 kb on - strand at 1829.806 kb on + strand at 1829.806 kb on + strand at 1829.806 kb on + strand at 1829.806 kb on + strand at 1829.806 kb on + strand at 1829.806 kb on + strand at 1829.806 kb on + strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.807 kb on - strand at 1829.813 kb on + strand at 1829.813 kb on + strand at 1829.813 kb on + strand at 1829.813 kb on + strand at 1829.813 kb on + strand at 1829.814 kb on - strand at 1829.814 kb on - strand at 1829.918 kb on + strand, within LRK53_RS08140 at 1829.918 kb on + strand, within LRK53_RS08140 at 1829.918 kb on + strand, within LRK53_RS08140 at 1829.919 kb on - strand, within LRK53_RS08140 at 1830.045 kb on + strand, within LRK53_RS08140 at 1830.045 kb on + strand, within LRK53_RS08140 at 1830.045 kb on + strand, within LRK53_RS08140 at 1830.045 kb on + strand, within LRK53_RS08140 at 1830.046 kb on - strand, within LRK53_RS08140 at 1830.046 kb on - strand, within LRK53_RS08140 at 1830.046 kb on - strand, within LRK53_RS08140 at 1830.265 kb on + strand at 1830.266 kb on - strand at 1830.266 kb on - strand at 1830.854 kb on + strand, within LRK53_RS08145 at 1830.854 kb on + strand, within LRK53_RS08145 at 1830.854 kb on + strand, within LRK53_RS08145 at 1830.855 kb on - strand, within LRK53_RS08145 at 1830.855 kb on - strand, within LRK53_RS08145 at 1830.855 kb on - strand, within LRK53_RS08145 at 1830.855 kb on - strand, within LRK53_RS08145 at 1830.945 kb on + strand, within LRK53_RS08145 at 1830.945 kb on + strand, within LRK53_RS08145 at 1830.946 kb on - strand, within LRK53_RS08145 at 1831.061 kb on + strand, within LRK53_RS08145 at 1831.061 kb on + strand, within LRK53_RS08145 at 1831.061 kb on + strand, within LRK53_RS08145 at 1831.062 kb on - strand, within LRK53_RS08145 at 1831.165 kb on + strand, within LRK53_RS08145 at 1831.165 kb on + strand, within LRK53_RS08145 at 1831.166 kb on - strand, within LRK53_RS08145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,828,809 + LRK53_RS08135 0.58 -0.8 1,828,809 + LRK53_RS08135 0.58 -2.0 1,828,809 + LRK53_RS08135 0.58 -0.6 1,828,810 - LRK53_RS08135 0.58 +1.0 1,828,810 - LRK53_RS08135 0.58 -1.0 1,828,810 - LRK53_RS08135 0.58 -2.0 1,828,810 - LRK53_RS08135 0.58 -1.0 1,828,859 + LRK53_RS08135 0.61 -0.2 1,828,859 + LRK53_RS08135 0.61 -1.4 1,828,859 + LRK53_RS08135 0.61 +0.0 1,828,859 + LRK53_RS08135 0.61 -1.2 1,828,859 + LRK53_RS08135 0.61 -0.6 1,828,860 - LRK53_RS08135 0.61 -0.6 1,828,860 - LRK53_RS08135 0.61 -1.3 1,828,860 - LRK53_RS08135 0.61 -1.1 1,828,860 - LRK53_RS08135 0.61 +0.4 1,828,860 - LRK53_RS08135 0.61 -0.8 1,828,860 - LRK53_RS08135 0.61 -2.4 1,828,860 - LRK53_RS08135 0.61 -2.0 1,828,860 - LRK53_RS08135 0.61 -0.1 1,828,860 - LRK53_RS08135 0.61 -1.5 1,828,860 - LRK53_RS08135 0.61 -1.2 1,828,860 - LRK53_RS08135 0.61 +0.4 1,828,860 - LRK53_RS08135 0.61 -0.1 1,828,861 - LRK53_RS08135 0.61 -1.6 1,828,883 + LRK53_RS08135 0.62 +0.1 1,828,903 + LRK53_RS08135 0.63 -1.0 1,828,903 + LRK53_RS08135 0.63 -0.7 1,828,903 + LRK53_RS08135 0.63 +2.0 1,828,903 + LRK53_RS08135 0.63 -0.6 1,828,903 + LRK53_RS08135 0.63 -1.6 1,828,903 + LRK53_RS08135 0.63 -0.5 1,828,903 + LRK53_RS08135 0.63 +0.0 1,828,903 + LRK53_RS08135 0.63 +2.0 1,828,903 + LRK53_RS08135 0.63 -0.6 1,828,904 - LRK53_RS08135 0.63 -1.3 1,828,904 - LRK53_RS08135 0.63 -1.0 1,828,904 - LRK53_RS08135 0.63 -0.2 1,828,904 - LRK53_RS08135 0.63 +0.0 1,828,904 - LRK53_RS08135 0.63 -1.5 1,828,904 - LRK53_RS08135 0.63 -1.6 1,828,904 - LRK53_RS08135 0.63 -0.6 1,828,904 - LRK53_RS08135 0.63 +0.0 1,828,904 - LRK53_RS08135 0.63 -0.2 1,828,904 - LRK53_RS08135 0.63 +0.0 1,828,991 + LRK53_RS08135 0.68 -1.0 1,828,991 + LRK53_RS08135 0.68 +0.0 1,828,992 - LRK53_RS08135 0.68 -1.8 1,828,992 - LRK53_RS08135 0.68 -1.0 1,829,102 + LRK53_RS08135 0.74 +0.0 1,829,103 - LRK53_RS08135 0.74 -1.2 1,829,103 - LRK53_RS08135 0.74 +0.0 1,829,103 - LRK53_RS08135 0.74 +0.4 1,829,103 - LRK53_RS08135 0.74 -0.9 1,829,108 + LRK53_RS08135 0.74 +0.0 1,829,109 - LRK53_RS08135 0.74 +0.5 1,829,194 + LRK53_RS08135 0.79 -2.8 1,829,283 - LRK53_RS08135 0.84 -0.6 1,829,283 - LRK53_RS08135 0.84 +1.6 1,829,312 + LRK53_RS08135 0.85 +1.0 1,829,312 + LRK53_RS08135 0.85 -2.2 1,829,312 + LRK53_RS08135 0.85 -1.0 1,829,312 + LRK53_RS08135 0.85 -0.4 1,829,312 + LRK53_RS08135 0.85 -0.4 1,829,312 + LRK53_RS08135 0.85 -0.7 1,829,312 + LRK53_RS08135 0.85 -0.6 1,829,313 - LRK53_RS08135 0.85 -1.0 1,829,313 - LRK53_RS08135 0.85 -1.8 1,829,313 - LRK53_RS08135 0.85 -0.8 1,829,480 + -0.0 1,829,481 - -1.0 1,829,556 + -3.0 1,829,557 - -0.0 1,829,557 - +1.0 1,829,575 + +1.2 1,829,577 + -1.3 1,829,577 + -1.5 1,829,577 + -0.5 1,829,577 + +0.6 1,829,577 + -0.8 1,829,577 + -0.5 1,829,577 + -1.4 1,829,578 - -1.9 1,829,578 - +2.0 1,829,578 - -0.6 1,829,578 - -0.1 1,829,578 - -0.1 1,829,578 - -0.6 1,829,578 - -0.7 1,829,578 - -0.0 1,829,578 - -1.0 1,829,786 + -1.0 1,829,787 - -1.5 1,829,787 - -1.0 1,829,787 - -0.4 1,829,787 - +0.4 1,829,806 + +1.3 1,829,806 + -2.7 1,829,806 + +1.0 1,829,806 + +1.0 1,829,806 + -2.3 1,829,806 + -1.2 1,829,806 + +0.7 1,829,807 - -1.2 1,829,807 - -1.0 1,829,807 - -0.0 1,829,807 - -0.0 1,829,807 - +1.5 1,829,807 - +1.0 1,829,807 - -0.2 1,829,807 - -0.0 1,829,813 + -0.6 1,829,813 + -0.7 1,829,813 + -0.9 1,829,813 + -0.0 1,829,813 + -1.0 1,829,814 - -1.3 1,829,814 - -1.0 1,829,918 + LRK53_RS08140 0.31 -1.0 1,829,918 + LRK53_RS08140 0.31 -0.9 1,829,918 + LRK53_RS08140 0.31 -2.0 1,829,919 - LRK53_RS08140 0.31 -1.3 1,830,045 + LRK53_RS08140 0.63 -1.3 1,830,045 + LRK53_RS08140 0.63 -2.1 1,830,045 + LRK53_RS08140 0.63 -1.4 1,830,045 + LRK53_RS08140 0.63 -0.0 1,830,046 - LRK53_RS08140 0.63 +0.4 1,830,046 - LRK53_RS08140 0.63 -0.4 1,830,046 - LRK53_RS08140 0.63 -0.9 1,830,265 + -2.7 1,830,266 - +0.6 1,830,266 - -0.0 1,830,854 + LRK53_RS08145 0.48 -1.1 1,830,854 + LRK53_RS08145 0.48 -0.4 1,830,854 + LRK53_RS08145 0.48 -1.6 1,830,855 - LRK53_RS08145 0.48 +0.6 1,830,855 - LRK53_RS08145 0.48 -0.5 1,830,855 - LRK53_RS08145 0.48 -0.0 1,830,855 - LRK53_RS08145 0.48 +0.6 1,830,945 + LRK53_RS08145 0.55 +1.0 1,830,945 + LRK53_RS08145 0.55 -1.0 1,830,946 - LRK53_RS08145 0.55 -0.8 1,831,061 + LRK53_RS08145 0.65 -1.1 1,831,061 + LRK53_RS08145 0.65 -0.3 1,831,061 + LRK53_RS08145 0.65 -2.3 1,831,062 - LRK53_RS08145 0.65 +0.4 1,831,165 + LRK53_RS08145 0.73 -1.2 1,831,165 + LRK53_RS08145 0.73 -1.0 1,831,166 - LRK53_RS08145 0.73 +0.3
Or see this region's nucleotide sequence