Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07975 and LRK53_RS07980 are separated by 196 nucleotides LRK53_RS07980 and LRK53_RS07985 overlap by 4 nucleotides LRK53_RS07985 and LRK53_RS07990 overlap by 4 nucleotides
LRK53_RS07975: LRK53_RS07975 - acyl-CoA dehydrogenase family protein, at 1,790,814 to 1,791,983
_RS07975
LRK53_RS07980: LRK53_RS07980 - GNAT family N-acetyltransferase, at 1,792,180 to 1,792,722
_RS07980
LRK53_RS07985: LRK53_RS07985 - hypothetical protein, at 1,792,719 to 1,793,201
_RS07985
LRK53_RS07990: LRK53_RS07990 - glutathione S-transferase family protein, at 1,793,198 to 1,793,809
_RS07990
Position (kb) 1792
1793 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1791.561 kb on + strand, within LRK53_RS07975 at 1791.562 kb on - strand, within LRK53_RS07975 at 1791.621 kb on + strand, within LRK53_RS07975 at 1791.622 kb on - strand, within LRK53_RS07975 at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.915 kb on + strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.916 kb on - strand at 1791.923 kb on + strand at 1792.100 kb on + strand at 1792.100 kb on + strand at 1792.100 kb on + strand at 1792.100 kb on + strand at 1792.101 kb on - strand at 1792.101 kb on - strand at 1792.101 kb on - strand at 1792.217 kb on + strand at 1792.218 kb on - strand at 1792.256 kb on - strand, within LRK53_RS07980 at 1792.256 kb on - strand, within LRK53_RS07980 at 1792.657 kb on + strand, within LRK53_RS07980 at 1792.658 kb on - strand, within LRK53_RS07980 at 1792.658 kb on - strand, within LRK53_RS07980 at 1792.658 kb on - strand, within LRK53_RS07980 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.962 kb on + strand, within LRK53_RS07985 at 1792.963 kb on - strand, within LRK53_RS07985 at 1792.963 kb on - strand, within LRK53_RS07985 at 1792.963 kb on - strand, within LRK53_RS07985 at 1792.963 kb on - strand, within LRK53_RS07985 at 1792.963 kb on - strand, within LRK53_RS07985 at 1793.174 kb on + strand at 1793.174 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.210 kb on + strand at 1793.211 kb on - strand at 1793.211 kb on - strand at 1793.211 kb on - strand at 1793.211 kb on - strand at 1793.237 kb on + strand at 1793.237 kb on + strand at 1793.237 kb on + strand at 1793.237 kb on + strand at 1793.237 kb on + strand at 1793.238 kb on - strand at 1793.238 kb on - strand at 1793.238 kb on - strand at 1793.238 kb on - strand at 1793.238 kb on - strand at 1793.238 kb on - strand at 1793.238 kb on - strand at 1793.327 kb on + strand, within LRK53_RS07990 at 1793.327 kb on + strand, within LRK53_RS07990 at 1793.327 kb on + strand, within LRK53_RS07990 at 1793.327 kb on + strand, within LRK53_RS07990 at 1793.328 kb on - strand, within LRK53_RS07990 at 1793.328 kb on - strand, within LRK53_RS07990 at 1793.328 kb on - strand, within LRK53_RS07990 at 1793.328 kb on - strand, within LRK53_RS07990 at 1793.355 kb on + strand, within LRK53_RS07990 at 1793.355 kb on + strand, within LRK53_RS07990 at 1793.355 kb on + strand, within LRK53_RS07990 at 1793.356 kb on - strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.495 kb on + strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.496 kb on - strand, within LRK53_RS07990 at 1793.684 kb on + strand, within LRK53_RS07990 at 1793.684 kb on + strand, within LRK53_RS07990 at 1793.685 kb on - strand, within LRK53_RS07990 at 1793.685 kb on - strand, within LRK53_RS07990 at 1793.685 kb on - strand, within LRK53_RS07990 at 1793.685 kb on - strand, within LRK53_RS07990 at 1793.685 kb on - strand, within LRK53_RS07990 at 1793.685 kb on - strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.690 kb on + strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990 at 1793.691 kb on - strand, within LRK53_RS07990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,791,561 + LRK53_RS07975 0.64 +0.6 1,791,562 - LRK53_RS07975 0.64 -1.0 1,791,621 + LRK53_RS07975 0.69 -0.1 1,791,622 - LRK53_RS07975 0.69 +0.0 1,791,915 + +0.3 1,791,915 + -1.6 1,791,915 + -1.0 1,791,915 + -1.4 1,791,915 + -0.2 1,791,915 + -0.6 1,791,915 + -2.0 1,791,915 + +0.0 1,791,915 + -0.8 1,791,915 + -0.7 1,791,915 + -1.5 1,791,915 + +0.0 1,791,915 + +2.6 1,791,916 - -1.3 1,791,916 - +1.0 1,791,916 - +0.7 1,791,916 - -0.8 1,791,916 - +0.4 1,791,916 - -0.2 1,791,916 - +1.4 1,791,916 - -2.1 1,791,916 - -0.1 1,791,916 - -1.0 1,791,916 - -0.9 1,791,916 - -3.2 1,791,916 - -0.6 1,791,923 + -0.6 1,792,100 + -0.9 1,792,100 + +0.6 1,792,100 + -0.7 1,792,100 + +0.1 1,792,101 - -0.5 1,792,101 - +0.7 1,792,101 - -1.7 1,792,217 + -0.8 1,792,218 - -1.7 1,792,256 - LRK53_RS07980 0.14 -1.5 1,792,256 - LRK53_RS07980 0.14 -0.6 1,792,657 + LRK53_RS07980 0.88 +0.6 1,792,658 - LRK53_RS07980 0.88 -0.6 1,792,658 - LRK53_RS07980 0.88 +0.6 1,792,658 - LRK53_RS07980 0.88 -1.0 1,792,962 + LRK53_RS07985 0.50 -1.1 1,792,962 + LRK53_RS07985 0.50 +1.6 1,792,962 + LRK53_RS07985 0.50 -1.3 1,792,962 + LRK53_RS07985 0.50 -0.7 1,792,962 + LRK53_RS07985 0.50 +0.0 1,792,962 + LRK53_RS07985 0.50 +0.7 1,792,962 + LRK53_RS07985 0.50 +0.0 1,792,962 + LRK53_RS07985 0.50 -1.3 1,792,963 - LRK53_RS07985 0.51 -0.8 1,792,963 - LRK53_RS07985 0.51 +0.0 1,792,963 - LRK53_RS07985 0.51 -1.2 1,792,963 - LRK53_RS07985 0.51 -0.7 1,792,963 - LRK53_RS07985 0.51 -0.2 1,793,174 + -1.6 1,793,174 + +0.4 1,793,210 + -1.0 1,793,210 + -1.6 1,793,210 + +1.0 1,793,210 + -4.6 1,793,210 + +0.6 1,793,210 + +1.0 1,793,210 + -0.2 1,793,210 + +0.4 1,793,210 + -1.2 1,793,210 + +0.4 1,793,210 + -0.6 1,793,210 + +1.0 1,793,210 + -0.4 1,793,210 + +0.7 1,793,211 - +0.8 1,793,211 - -2.8 1,793,211 - +1.0 1,793,211 - +0.0 1,793,237 + +1.0 1,793,237 + +0.4 1,793,237 + +1.6 1,793,237 + +2.0 1,793,237 + -1.0 1,793,238 - -0.6 1,793,238 - -0.6 1,793,238 - +0.0 1,793,238 - -2.0 1,793,238 - -1.6 1,793,238 - +0.4 1,793,238 - +1.0 1,793,327 + LRK53_RS07990 0.21 -0.9 1,793,327 + LRK53_RS07990 0.21 -2.5 1,793,327 + LRK53_RS07990 0.21 -0.8 1,793,327 + LRK53_RS07990 0.21 +0.8 1,793,328 - LRK53_RS07990 0.21 +1.0 1,793,328 - LRK53_RS07990 0.21 -0.8 1,793,328 - LRK53_RS07990 0.21 +0.4 1,793,328 - LRK53_RS07990 0.21 -0.7 1,793,355 + LRK53_RS07990 0.26 -0.9 1,793,355 + LRK53_RS07990 0.26 +1.0 1,793,355 + LRK53_RS07990 0.26 -0.9 1,793,356 - LRK53_RS07990 0.26 +0.0 1,793,495 + LRK53_RS07990 0.49 -0.3 1,793,495 + LRK53_RS07990 0.49 +0.0 1,793,495 + LRK53_RS07990 0.49 +0.0 1,793,495 + LRK53_RS07990 0.49 +0.4 1,793,495 + LRK53_RS07990 0.49 -0.9 1,793,495 + LRK53_RS07990 0.49 -2.0 1,793,495 + LRK53_RS07990 0.49 -1.3 1,793,495 + LRK53_RS07990 0.49 +0.6 1,793,495 + LRK53_RS07990 0.49 -1.0 1,793,495 + LRK53_RS07990 0.49 -1.4 1,793,495 + LRK53_RS07990 0.49 -0.7 1,793,495 + LRK53_RS07990 0.49 -1.7 1,793,495 + LRK53_RS07990 0.49 -0.3 1,793,495 + LRK53_RS07990 0.49 +0.0 1,793,495 + LRK53_RS07990 0.49 -1.2 1,793,495 + LRK53_RS07990 0.49 -2.0 1,793,496 - LRK53_RS07990 0.49 -0.6 1,793,496 - LRK53_RS07990 0.49 -0.1 1,793,496 - LRK53_RS07990 0.49 -0.5 1,793,496 - LRK53_RS07990 0.49 -1.2 1,793,496 - LRK53_RS07990 0.49 -1.2 1,793,496 - LRK53_RS07990 0.49 -1.0 1,793,496 - LRK53_RS07990 0.49 +0.0 1,793,496 - LRK53_RS07990 0.49 +2.6 1,793,496 - LRK53_RS07990 0.49 +0.0 1,793,684 + LRK53_RS07990 0.79 -0.1 1,793,684 + LRK53_RS07990 0.79 -0.6 1,793,685 - LRK53_RS07990 0.80 -2.0 1,793,685 - LRK53_RS07990 0.80 -1.2 1,793,685 - LRK53_RS07990 0.80 -0.2 1,793,685 - LRK53_RS07990 0.80 -1.2 1,793,685 - LRK53_RS07990 0.80 +0.2 1,793,685 - LRK53_RS07990 0.80 +0.0 1,793,690 + LRK53_RS07990 0.80 -0.3 1,793,690 + LRK53_RS07990 0.80 +0.0 1,793,690 + LRK53_RS07990 0.80 +1.0 1,793,690 + LRK53_RS07990 0.80 +0.3 1,793,690 + LRK53_RS07990 0.80 -0.3 1,793,690 + LRK53_RS07990 0.80 -0.4 1,793,690 + LRK53_RS07990 0.80 -0.3 1,793,690 + LRK53_RS07990 0.80 -1.6 1,793,690 + LRK53_RS07990 0.80 -1.0 1,793,690 + LRK53_RS07990 0.80 +1.0 1,793,691 - LRK53_RS07990 0.81 +0.8 1,793,691 - LRK53_RS07990 0.81 +0.0 1,793,691 - LRK53_RS07990 0.81 -0.6 1,793,691 - LRK53_RS07990 0.81 -0.6 1,793,691 - LRK53_RS07990 0.81 -0.8 1,793,691 - LRK53_RS07990 0.81 -0.6 1,793,691 - LRK53_RS07990 0.81 +0.4 1,793,691 - LRK53_RS07990 0.81 +0.0
Or see this region's nucleotide sequence