Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07590 and LRK53_RS07595 are separated by 179 nucleotides LRK53_RS07595 and LRK53_RS07600 are separated by 150 nucleotides LRK53_RS07600 and LRK53_RS07605 overlap by 1 nucleotides LRK53_RS07605 and LRK53_RS07610 overlap by 1 nucleotides
LRK53_RS07590: LRK53_RS07590 - hypothetical protein, at 1,696,200 to 1,697,987
_RS07590
LRK53_RS07595: LRK53_RS07595 - hypothetical protein, at 1,698,167 to 1,698,409
_RS07595
LRK53_RS07600: LRK53_RS07600 - ABC transporter ATP-binding protein, at 1,698,560 to 1,699,255
_RS07600
LRK53_RS07605: LRK53_RS07605 - efflux RND transporter periplasmic adaptor subunit, at 1,699,255 to 1,700,193
_RS07605
LRK53_RS07610: LRK53_RS07610 - FtsX-like permease family protein, at 1,700,193 to 1,701,383
_RS07610
Position (kb)
1698
1699
1700 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1697.691 kb on - strand, within LRK53_RS07590 at 1697.691 kb on - strand, within LRK53_RS07590 at 1697.795 kb on - strand, within LRK53_RS07590 at 1697.795 kb on - strand, within LRK53_RS07590 at 1697.805 kb on + strand, within LRK53_RS07590 at 1697.805 kb on + strand, within LRK53_RS07590 at 1697.806 kb on - strand, within LRK53_RS07590 at 1697.806 kb on - strand, within LRK53_RS07590 at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.811 kb on + strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.812 kb on - strand at 1697.814 kb on + strand at 1697.814 kb on + strand at 1697.815 kb on - strand at 1697.815 kb on - strand at 1697.815 kb on - strand at 1697.815 kb on - strand at 1697.898 kb on + strand at 1697.898 kb on + strand at 1697.898 kb on + strand at 1697.898 kb on + strand at 1697.899 kb on - strand at 1698.018 kb on + strand at 1698.018 kb on + strand at 1698.019 kb on - strand at 1698.019 kb on - strand at 1698.019 kb on - strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.020 kb on + strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.021 kb on - strand at 1698.023 kb on - strand at 1698.093 kb on + strand at 1698.093 kb on + strand at 1698.093 kb on + strand at 1698.094 kb on - strand at 1698.094 kb on - strand at 1698.493 kb on + strand at 1698.494 kb on - strand at 1698.494 kb on - strand at 1698.494 kb on - strand at 1698.509 kb on + strand at 1698.509 kb on + strand at 1698.509 kb on + strand at 1698.509 kb on + strand at 1698.509 kb on + strand at 1698.510 kb on - strand at 1698.510 kb on - strand at 1698.510 kb on - strand at 1698.587 kb on + strand at 1698.587 kb on + strand at 1698.587 kb on + strand at 1698.587 kb on + strand at 1698.587 kb on + strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1698.588 kb on - strand at 1699.176 kb on - strand, within LRK53_RS07600 at 1699.279 kb on + strand at 1699.279 kb on + strand at 1699.279 kb on + strand at 1699.279 kb on + strand at 1699.280 kb on - strand at 1699.280 kb on - strand at 1699.280 kb on - strand at 1699.280 kb on - strand at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.402 kb on + strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.403 kb on - strand, within LRK53_RS07605 at 1699.630 kb on + strand, within LRK53_RS07605 at 1699.630 kb on + strand, within LRK53_RS07605 at 1699.631 kb on - strand, within LRK53_RS07605 at 1699.631 kb on - strand, within LRK53_RS07605 at 1699.631 kb on - strand, within LRK53_RS07605 at 1699.631 kb on - strand, within LRK53_RS07605 at 1699.631 kb on - strand, within LRK53_RS07605 at 1699.631 kb on - strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.894 kb on + strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1699.895 kb on - strand, within LRK53_RS07605 at 1700.005 kb on + strand, within LRK53_RS07605 at 1700.005 kb on + strand, within LRK53_RS07605 at 1700.006 kb on - strand, within LRK53_RS07605 at 1700.006 kb on - strand, within LRK53_RS07605 at 1700.006 kb on - strand, within LRK53_RS07605 at 1700.006 kb on - strand, within LRK53_RS07605 at 1700.006 kb on - strand, within LRK53_RS07605 at 1700.006 kb on - strand, within LRK53_RS07605 at 1700.017 kb on + strand, within LRK53_RS07605 at 1700.017 kb on + strand, within LRK53_RS07605 at 1700.017 kb on + strand, within LRK53_RS07605 at 1700.017 kb on + strand, within LRK53_RS07605 at 1700.017 kb on + strand, within LRK53_RS07605 at 1700.017 kb on + strand, within LRK53_RS07605 at 1700.018 kb on - strand, within LRK53_RS07605 at 1700.018 kb on - strand, within LRK53_RS07605 at 1700.018 kb on - strand, within LRK53_RS07605 at 1700.018 kb on - strand, within LRK53_RS07605 at 1700.018 kb on - strand, within LRK53_RS07605 at 1700.018 kb on - strand, within LRK53_RS07605 at 1700.089 kb on + strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.090 kb on - strand, within LRK53_RS07605 at 1700.119 kb on + strand at 1700.120 kb on - strand at 1700.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,697,691 - LRK53_RS07590 0.83 +1.0 1,697,691 - LRK53_RS07590 0.83 +0.7 1,697,795 - LRK53_RS07590 0.89 +1.0 1,697,795 - LRK53_RS07590 0.89 -1.4 1,697,805 + LRK53_RS07590 0.90 -1.3 1,697,805 + LRK53_RS07590 0.90 -0.8 1,697,806 - LRK53_RS07590 0.90 -2.0 1,697,806 - LRK53_RS07590 0.90 -1.3 1,697,811 + +1.0 1,697,811 + +0.3 1,697,811 + -0.8 1,697,811 + +2.6 1,697,811 + -2.1 1,697,811 + -0.6 1,697,811 + -0.6 1,697,811 + +0.0 1,697,812 - -0.6 1,697,812 - -1.6 1,697,812 - -1.3 1,697,812 - -0.8 1,697,812 - +0.2 1,697,812 - -1.2 1,697,812 - -0.8 1,697,812 - +1.0 1,697,812 - -1.0 1,697,812 - -1.4 1,697,812 - -0.3 1,697,814 + +2.0 1,697,814 + +2.0 1,697,815 - -0.6 1,697,815 - +0.0 1,697,815 - -1.0 1,697,815 - +1.0 1,697,898 + -0.3 1,697,898 + -0.6 1,697,898 + -3.6 1,697,898 + -0.9 1,697,899 - -0.3 1,698,018 + -1.0 1,698,018 + +1.0 1,698,019 - +0.0 1,698,019 - -1.0 1,698,019 - +0.8 1,698,020 + -1.7 1,698,020 + -0.4 1,698,020 + +0.4 1,698,020 + -0.0 1,698,020 + -0.5 1,698,020 + +1.0 1,698,020 + -0.2 1,698,020 + -1.0 1,698,020 + -1.4 1,698,020 + +0.0 1,698,020 + -0.3 1,698,020 + -0.1 1,698,020 + +1.0 1,698,020 + +0.0 1,698,021 - -1.1 1,698,021 - -0.3 1,698,021 - +0.7 1,698,021 - +1.4 1,698,021 - +1.4 1,698,021 - -0.3 1,698,021 - -0.8 1,698,021 - -1.5 1,698,023 - -1.0 1,698,093 + +0.4 1,698,093 + -1.2 1,698,093 + -1.0 1,698,094 - -1.8 1,698,094 - -0.7 1,698,493 + +0.0 1,698,494 - +2.6 1,698,494 - +1.4 1,698,494 - -1.6 1,698,509 + +0.0 1,698,509 + +1.0 1,698,509 + +0.0 1,698,509 + -0.6 1,698,509 + +0.0 1,698,510 - -1.0 1,698,510 - -0.2 1,698,510 - +0.3 1,698,587 + +2.0 1,698,587 + -1.4 1,698,587 + -0.5 1,698,587 + +1.0 1,698,587 + -0.5 1,698,588 - +0.0 1,698,588 - +1.0 1,698,588 - -2.0 1,698,588 - -0.1 1,698,588 - -1.8 1,698,588 - -0.6 1,698,588 - +0.0 1,698,588 - -0.4 1,699,176 - LRK53_RS07600 0.89 -1.2 1,699,279 + +0.0 1,699,279 + -1.5 1,699,279 + -1.3 1,699,279 + -0.1 1,699,280 - -0.4 1,699,280 - +0.0 1,699,280 - +0.0 1,699,280 - -1.3 1,699,402 + LRK53_RS07605 0.16 -2.4 1,699,402 + LRK53_RS07605 0.16 -0.1 1,699,402 + LRK53_RS07605 0.16 -1.8 1,699,402 + LRK53_RS07605 0.16 +1.3 1,699,402 + LRK53_RS07605 0.16 -0.8 1,699,402 + LRK53_RS07605 0.16 +0.4 1,699,402 + LRK53_RS07605 0.16 -0.7 1,699,402 + LRK53_RS07605 0.16 -1.3 1,699,402 + LRK53_RS07605 0.16 -1.3 1,699,402 + LRK53_RS07605 0.16 -0.9 1,699,402 + LRK53_RS07605 0.16 +0.4 1,699,402 + LRK53_RS07605 0.16 +0.0 1,699,403 - LRK53_RS07605 0.16 -1.3 1,699,403 - LRK53_RS07605 0.16 +0.2 1,699,403 - LRK53_RS07605 0.16 +0.8 1,699,403 - LRK53_RS07605 0.16 -2.0 1,699,403 - LRK53_RS07605 0.16 +0.0 1,699,403 - LRK53_RS07605 0.16 +0.0 1,699,403 - LRK53_RS07605 0.16 +0.4 1,699,403 - LRK53_RS07605 0.16 -0.2 1,699,630 + LRK53_RS07605 0.40 -1.0 1,699,630 + LRK53_RS07605 0.40 -0.7 1,699,631 - LRK53_RS07605 0.40 -0.1 1,699,631 - LRK53_RS07605 0.40 -0.2 1,699,631 - LRK53_RS07605 0.40 +0.0 1,699,631 - LRK53_RS07605 0.40 -0.6 1,699,631 - LRK53_RS07605 0.40 -0.9 1,699,631 - LRK53_RS07605 0.40 -0.6 1,699,894 + LRK53_RS07605 0.68 -1.0 1,699,894 + LRK53_RS07605 0.68 -0.2 1,699,894 + LRK53_RS07605 0.68 -0.6 1,699,894 + LRK53_RS07605 0.68 -1.3 1,699,894 + LRK53_RS07605 0.68 +0.2 1,699,894 + LRK53_RS07605 0.68 +1.0 1,699,894 + LRK53_RS07605 0.68 +1.0 1,699,894 + LRK53_RS07605 0.68 -3.1 1,699,894 + LRK53_RS07605 0.68 +0.0 1,699,894 + LRK53_RS07605 0.68 -0.4 1,699,894 + LRK53_RS07605 0.68 -2.4 1,699,894 + LRK53_RS07605 0.68 +0.0 1,699,894 + LRK53_RS07605 0.68 -2.7 1,699,894 + LRK53_RS07605 0.68 +0.0 1,699,895 - LRK53_RS07605 0.68 +1.0 1,699,895 - LRK53_RS07605 0.68 -0.5 1,699,895 - LRK53_RS07605 0.68 -1.6 1,699,895 - LRK53_RS07605 0.68 +0.4 1,699,895 - LRK53_RS07605 0.68 -2.3 1,699,895 - LRK53_RS07605 0.68 -2.2 1,699,895 - LRK53_RS07605 0.68 +0.0 1,699,895 - LRK53_RS07605 0.68 -1.2 1,699,895 - LRK53_RS07605 0.68 +0.0 1,699,895 - LRK53_RS07605 0.68 -0.6 1,699,895 - LRK53_RS07605 0.68 -0.2 1,700,005 + LRK53_RS07605 0.80 -2.3 1,700,005 + LRK53_RS07605 0.80 +0.0 1,700,006 - LRK53_RS07605 0.80 +0.0 1,700,006 - LRK53_RS07605 0.80 -1.0 1,700,006 - LRK53_RS07605 0.80 +0.0 1,700,006 - LRK53_RS07605 0.80 +0.4 1,700,006 - LRK53_RS07605 0.80 -0.2 1,700,006 - LRK53_RS07605 0.80 +0.0 1,700,017 + LRK53_RS07605 0.81 -1.0 1,700,017 + LRK53_RS07605 0.81 +1.0 1,700,017 + LRK53_RS07605 0.81 +1.0 1,700,017 + LRK53_RS07605 0.81 -0.8 1,700,017 + LRK53_RS07605 0.81 -0.2 1,700,017 + LRK53_RS07605 0.81 +0.3 1,700,018 - LRK53_RS07605 0.81 -0.6 1,700,018 - LRK53_RS07605 0.81 -2.2 1,700,018 - LRK53_RS07605 0.81 -1.8 1,700,018 - LRK53_RS07605 0.81 +0.8 1,700,018 - LRK53_RS07605 0.81 -0.2 1,700,018 - LRK53_RS07605 0.81 -0.6 1,700,089 + LRK53_RS07605 0.89 +1.0 1,700,090 - LRK53_RS07605 0.89 -1.8 1,700,090 - LRK53_RS07605 0.89 +0.5 1,700,090 - LRK53_RS07605 0.89 +0.6 1,700,090 - LRK53_RS07605 0.89 -1.8 1,700,090 - LRK53_RS07605 0.89 -1.2 1,700,090 - LRK53_RS07605 0.89 +0.0 1,700,090 - LRK53_RS07605 0.89 +0.2 1,700,119 + +2.0 1,700,120 - +0.7 1,700,120 - -0.6
Or see this region's nucleotide sequence