Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07480 and LRK53_RS07485 overlap by 4 nucleotides LRK53_RS07485 and LRK53_RS07490 overlap by 4 nucleotides LRK53_RS07490 and LRK53_RS07495 are separated by 109 nucleotides
LRK53_RS07480: LRK53_RS07480 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 1,672,669 to 1,673,397
_RS07480
LRK53_RS07485: LRK53_RS07485 - phosphoglycolate phosphatase, at 1,673,394 to 1,674,074
_RS07485
LRK53_RS07490: LRK53_RS07490 - phytoene synthase, at 1,674,071 to 1,674,925
_RS07490
LRK53_RS07495: LRK53_RS07495 - GTP cyclohydrolase FolE2, at 1,675,035 to 1,675,982
_RS07495
Position (kb)
1674
1675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1673.377 kb on + strand at 1673.377 kb on + strand at 1673.378 kb on - strand at 1673.378 kb on - strand at 1673.378 kb on - strand at 1673.473 kb on - strand, within LRK53_RS07485 at 1673.473 kb on - strand, within LRK53_RS07485 at 1673.473 kb on - strand, within LRK53_RS07485 at 1673.524 kb on - strand, within LRK53_RS07485 at 1673.524 kb on - strand, within LRK53_RS07485 at 1673.625 kb on + strand, within LRK53_RS07485 at 1673.625 kb on + strand, within LRK53_RS07485 at 1673.626 kb on - strand, within LRK53_RS07485 at 1673.626 kb on - strand, within LRK53_RS07485 at 1673.626 kb on - strand, within LRK53_RS07485 at 1673.637 kb on + strand, within LRK53_RS07485 at 1673.637 kb on + strand, within LRK53_RS07485 at 1673.637 kb on + strand, within LRK53_RS07485 at 1673.638 kb on - strand, within LRK53_RS07485 at 1673.638 kb on - strand, within LRK53_RS07485 at 1673.638 kb on - strand, within LRK53_RS07485 at 1673.638 kb on - strand, within LRK53_RS07485 at 1673.923 kb on + strand, within LRK53_RS07485 at 1673.924 kb on - strand, within LRK53_RS07485 at 1673.924 kb on - strand, within LRK53_RS07485 at 1673.949 kb on + strand, within LRK53_RS07485 at 1673.950 kb on - strand, within LRK53_RS07485 at 1673.950 kb on - strand, within LRK53_RS07485 at 1674.100 kb on - strand at 1674.122 kb on + strand at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.506 kb on + strand, within LRK53_RS07490 at 1674.664 kb on + strand, within LRK53_RS07490 at 1674.665 kb on - strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.837 kb on + strand, within LRK53_RS07490 at 1674.838 kb on - strand, within LRK53_RS07490 at 1674.838 kb on - strand, within LRK53_RS07490 at 1674.914 kb on + strand at 1674.915 kb on - strand at 1674.915 kb on - strand at 1674.923 kb on + strand at 1674.923 kb on + strand at 1674.923 kb on + strand at 1674.924 kb on - strand at 1674.924 kb on - strand at 1674.960 kb on + strand at 1674.960 kb on + strand at 1674.960 kb on + strand at 1674.961 kb on - strand at 1674.961 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,673,377 + -0.8 1,673,377 + -1.2 1,673,378 - -0.6 1,673,378 - +0.3 1,673,378 - -0.1 1,673,473 - LRK53_RS07485 0.12 +0.4 1,673,473 - LRK53_RS07485 0.12 +0.4 1,673,473 - LRK53_RS07485 0.12 -1.8 1,673,524 - LRK53_RS07485 0.19 -1.1 1,673,524 - LRK53_RS07485 0.19 -1.3 1,673,625 + LRK53_RS07485 0.34 +1.0 1,673,625 + LRK53_RS07485 0.34 +0.4 1,673,626 - LRK53_RS07485 0.34 -1.3 1,673,626 - LRK53_RS07485 0.34 +0.0 1,673,626 - LRK53_RS07485 0.34 +0.0 1,673,637 + LRK53_RS07485 0.36 -2.3 1,673,637 + LRK53_RS07485 0.36 -0.4 1,673,637 + LRK53_RS07485 0.36 -2.2 1,673,638 - LRK53_RS07485 0.36 -0.5 1,673,638 - LRK53_RS07485 0.36 -1.3 1,673,638 - LRK53_RS07485 0.36 +0.0 1,673,638 - LRK53_RS07485 0.36 +0.0 1,673,923 + LRK53_RS07485 0.78 -0.4 1,673,924 - LRK53_RS07485 0.78 -0.6 1,673,924 - LRK53_RS07485 0.78 +0.2 1,673,949 + LRK53_RS07485 0.81 +0.4 1,673,950 - LRK53_RS07485 0.82 +0.6 1,673,950 - LRK53_RS07485 0.82 -1.9 1,674,100 - +3.3 1,674,122 + -0.6 1,674,506 + LRK53_RS07490 0.51 +1.0 1,674,506 + LRK53_RS07490 0.51 -2.0 1,674,506 + LRK53_RS07490 0.51 +1.0 1,674,506 + LRK53_RS07490 0.51 +1.0 1,674,506 + LRK53_RS07490 0.51 -1.2 1,674,506 + LRK53_RS07490 0.51 -2.8 1,674,506 + LRK53_RS07490 0.51 -0.5 1,674,664 + LRK53_RS07490 0.69 +1.0 1,674,665 - LRK53_RS07490 0.69 +0.0 1,674,837 + LRK53_RS07490 0.90 -0.6 1,674,837 + LRK53_RS07490 0.90 -0.6 1,674,837 + LRK53_RS07490 0.90 -1.0 1,674,837 + LRK53_RS07490 0.90 -1.0 1,674,837 + LRK53_RS07490 0.90 +0.0 1,674,837 + LRK53_RS07490 0.90 -0.6 1,674,837 + LRK53_RS07490 0.90 -3.1 1,674,837 + LRK53_RS07490 0.90 -0.7 1,674,838 - LRK53_RS07490 0.90 -1.8 1,674,838 - LRK53_RS07490 0.90 -2.6 1,674,914 + -1.1 1,674,915 - -1.0 1,674,915 - -0.9 1,674,923 + -1.6 1,674,923 + -0.8 1,674,923 + -0.6 1,674,924 - -0.8 1,674,924 - -1.2 1,674,960 + +0.0 1,674,960 + -1.6 1,674,960 + +0.4 1,674,961 - -1.4 1,674,961 - -1.1
Or see this region's nucleotide sequence