Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07460 and LRK53_RS07465 are separated by 117 nucleotides LRK53_RS07465 and LRK53_RS07470 are separated by 232 nucleotides LRK53_RS07470 and LRK53_RS07475 are separated by 14 nucleotides LRK53_RS07475 and LRK53_RS07480 are separated by 78 nucleotides
LRK53_RS07460: LRK53_RS07460 - EF-P beta-lysylation protein EpmB, at 1,669,057 to 1,670,073
_RS07460
LRK53_RS07465: LRK53_RS07465 - elongation factor P, at 1,670,191 to 1,670,757
_RS07465
LRK53_RS07470: LRK53_RS07470 - TRZ/ATZ family hydrolase, at 1,670,990 to 1,672,321
_RS07470
LRK53_RS07475: LRK53_RS07475 - YciI family protein, at 1,672,336 to 1,672,590
_RS07475
LRK53_RS07480: LRK53_RS07480 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 1,672,669 to 1,673,397
_RS07480
Position (kb)
1670
1671
1672
1673 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.113 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.173 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.175 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.830 kb on + strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.831 kb on - strand at 1670.890 kb on + strand at 1670.891 kb on - strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.962 kb on + strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.963 kb on - strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.966 kb on + strand at 1670.967 kb on - strand at 1670.967 kb on - strand at 1671.147 kb on - strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.306 kb on + strand, within LRK53_RS07470 at 1671.307 kb on - strand, within LRK53_RS07470 at 1671.307 kb on - strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.392 kb on + strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.393 kb on - strand, within LRK53_RS07470 at 1671.422 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.503 kb on + strand, within LRK53_RS07470 at 1671.504 kb on - strand, within LRK53_RS07470 at 1671.504 kb on - strand, within LRK53_RS07470 at 1671.504 kb on - strand, within LRK53_RS07470 at 1671.504 kb on - strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.527 kb on + strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.528 kb on - strand, within LRK53_RS07470 at 1671.584 kb on + strand, within LRK53_RS07470 at 1671.584 kb on + strand, within LRK53_RS07470 at 1671.584 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.586 kb on + strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1671.587 kb on - strand, within LRK53_RS07470 at 1672.032 kb on + strand, within LRK53_RS07470 at 1672.033 kb on - strand, within LRK53_RS07470 at 1672.033 kb on - strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.181 kb on + strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.182 kb on - strand, within LRK53_RS07470 at 1672.264 kb on + strand at 1672.345 kb on + strand at 1672.346 kb on - strand at 1672.346 kb on - strand at 1672.346 kb on - strand at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.480 kb on + strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.481 kb on - strand, within LRK53_RS07475 at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.570 kb on + strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.571 kb on - strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.639 kb on + strand at 1672.640 kb on - strand at 1672.640 kb on - strand at 1672.640 kb on - strand at 1672.640 kb on - strand at 1672.640 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,670,113 + -3.1 1,670,113 + -1.0 1,670,113 + -1.3 1,670,113 + -0.1 1,670,113 + +1.0 1,670,113 + +0.4 1,670,113 + +1.0 1,670,113 + +0.2 1,670,113 + -0.7 1,670,113 + -0.3 1,670,113 + -1.7 1,670,113 + +1.0 1,670,173 + -1.3 1,670,173 + +2.6 1,670,173 + +1.7 1,670,173 + -0.8 1,670,173 + +1.6 1,670,173 + -1.0 1,670,173 + -0.6 1,670,173 + +0.8 1,670,173 + +0.0 1,670,175 + -0.2 1,670,175 + -1.0 1,670,175 + +0.7 1,670,175 + -0.6 1,670,175 + -2.7 1,670,175 + -1.0 1,670,175 + +0.2 1,670,175 + +0.4 1,670,175 + +0.0 1,670,175 + -2.8 1,670,830 + -1.3 1,670,830 + -0.6 1,670,830 + +0.0 1,670,830 + +1.0 1,670,830 + -0.8 1,670,830 + +1.6 1,670,830 + +0.7 1,670,830 + +0.0 1,670,830 + +0.3 1,670,830 + +0.0 1,670,830 + -1.4 1,670,830 + +0.2 1,670,831 - +1.0 1,670,831 - -1.6 1,670,831 - +1.0 1,670,831 - -0.3 1,670,831 - -0.8 1,670,831 - -2.2 1,670,831 - +0.0 1,670,890 + -0.7 1,670,891 - +0.3 1,670,962 + -1.1 1,670,962 + -0.5 1,670,962 + -0.7 1,670,962 + +0.0 1,670,962 + +0.0 1,670,962 + +0.3 1,670,962 + -0.4 1,670,962 + -2.0 1,670,962 + -0.1 1,670,962 + -0.6 1,670,962 + -0.3 1,670,962 + -0.1 1,670,962 + +2.0 1,670,962 + -2.2 1,670,962 + -1.2 1,670,962 + -2.0 1,670,963 - -0.6 1,670,963 - +1.0 1,670,963 - +0.0 1,670,963 - -0.7 1,670,963 - +0.3 1,670,963 - +0.6 1,670,963 - +0.0 1,670,963 - -2.4 1,670,963 - -0.2 1,670,963 - -1.8 1,670,963 - +0.0 1,670,963 - -0.4 1,670,963 - -0.3 1,670,963 - -0.1 1,670,963 - -0.5 1,670,966 + -0.6 1,670,966 + -1.8 1,670,966 + -1.6 1,670,966 + -0.6 1,670,966 + +1.3 1,670,966 + +0.0 1,670,966 + -1.3 1,670,966 + +1.0 1,670,966 + +0.3 1,670,967 - +1.0 1,670,967 - +0.0 1,671,147 - LRK53_RS07470 0.12 +1.6 1,671,306 + LRK53_RS07470 0.24 -1.3 1,671,306 + LRK53_RS07470 0.24 -0.8 1,671,306 + LRK53_RS07470 0.24 -1.5 1,671,306 + LRK53_RS07470 0.24 -0.6 1,671,306 + LRK53_RS07470 0.24 -1.6 1,671,306 + LRK53_RS07470 0.24 +0.0 1,671,306 + LRK53_RS07470 0.24 -0.6 1,671,307 - LRK53_RS07470 0.24 -0.6 1,671,307 - LRK53_RS07470 0.24 -0.6 1,671,392 + LRK53_RS07470 0.30 +1.0 1,671,392 + LRK53_RS07470 0.30 +0.6 1,671,392 + LRK53_RS07470 0.30 -0.4 1,671,392 + LRK53_RS07470 0.30 -0.2 1,671,392 + LRK53_RS07470 0.30 +0.6 1,671,392 + LRK53_RS07470 0.30 -1.6 1,671,392 + LRK53_RS07470 0.30 -0.6 1,671,392 + LRK53_RS07470 0.30 -0.4 1,671,392 + LRK53_RS07470 0.30 +0.0 1,671,392 + LRK53_RS07470 0.30 -1.6 1,671,392 + LRK53_RS07470 0.30 -1.0 1,671,392 + LRK53_RS07470 0.30 +1.0 1,671,392 + LRK53_RS07470 0.30 -1.3 1,671,392 + LRK53_RS07470 0.30 +1.0 1,671,393 - LRK53_RS07470 0.30 +1.0 1,671,393 - LRK53_RS07470 0.30 +1.0 1,671,393 - LRK53_RS07470 0.30 +0.6 1,671,393 - LRK53_RS07470 0.30 -1.0 1,671,393 - LRK53_RS07470 0.30 +1.0 1,671,393 - LRK53_RS07470 0.30 -1.3 1,671,393 - LRK53_RS07470 0.30 +1.0 1,671,393 - LRK53_RS07470 0.30 +0.6 1,671,422 + LRK53_RS07470 0.32 -2.3 1,671,503 + LRK53_RS07470 0.39 -1.0 1,671,503 + LRK53_RS07470 0.39 +0.4 1,671,503 + LRK53_RS07470 0.39 +2.0 1,671,503 + LRK53_RS07470 0.39 -0.6 1,671,503 + LRK53_RS07470 0.39 +0.0 1,671,504 - LRK53_RS07470 0.39 +0.0 1,671,504 - LRK53_RS07470 0.39 -0.6 1,671,504 - LRK53_RS07470 0.39 +1.0 1,671,504 - LRK53_RS07470 0.39 -1.3 1,671,527 + LRK53_RS07470 0.40 -0.6 1,671,527 + LRK53_RS07470 0.40 +1.6 1,671,527 + LRK53_RS07470 0.40 -1.0 1,671,527 + LRK53_RS07470 0.40 -1.0 1,671,527 + LRK53_RS07470 0.40 +0.0 1,671,527 + LRK53_RS07470 0.40 +1.0 1,671,527 + LRK53_RS07470 0.40 +1.6 1,671,527 + LRK53_RS07470 0.40 +1.0 1,671,527 + LRK53_RS07470 0.40 -0.6 1,671,527 + LRK53_RS07470 0.40 -1.4 1,671,527 + LRK53_RS07470 0.40 +0.0 1,671,527 + LRK53_RS07470 0.40 -0.2 1,671,528 - LRK53_RS07470 0.40 -1.3 1,671,528 - LRK53_RS07470 0.40 -1.3 1,671,528 - LRK53_RS07470 0.40 -0.6 1,671,528 - LRK53_RS07470 0.40 -1.0 1,671,528 - LRK53_RS07470 0.40 -0.6 1,671,528 - LRK53_RS07470 0.40 +0.7 1,671,584 + LRK53_RS07470 0.45 -1.8 1,671,584 + LRK53_RS07470 0.45 -1.0 1,671,584 + LRK53_RS07470 0.45 +1.0 1,671,586 + LRK53_RS07470 0.45 +1.0 1,671,586 + LRK53_RS07470 0.45 -0.3 1,671,586 + LRK53_RS07470 0.45 -2.6 1,671,586 + LRK53_RS07470 0.45 -2.7 1,671,586 + LRK53_RS07470 0.45 +1.6 1,671,586 + LRK53_RS07470 0.45 +0.0 1,671,586 + LRK53_RS07470 0.45 +0.0 1,671,586 + LRK53_RS07470 0.45 -1.2 1,671,586 + LRK53_RS07470 0.45 +0.0 1,671,586 + LRK53_RS07470 0.45 -0.6 1,671,586 + LRK53_RS07470 0.45 +0.0 1,671,586 + LRK53_RS07470 0.45 -1.6 1,671,586 + LRK53_RS07470 0.45 -2.4 1,671,587 - LRK53_RS07470 0.45 +0.0 1,671,587 - LRK53_RS07470 0.45 -1.5 1,671,587 - LRK53_RS07470 0.45 -0.6 1,671,587 - LRK53_RS07470 0.45 -1.0 1,671,587 - LRK53_RS07470 0.45 -0.9 1,671,587 - LRK53_RS07470 0.45 -1.3 1,671,587 - LRK53_RS07470 0.45 -0.4 1,671,587 - LRK53_RS07470 0.45 +0.0 1,671,587 - LRK53_RS07470 0.45 -1.0 1,671,587 - LRK53_RS07470 0.45 +0.4 1,671,587 - LRK53_RS07470 0.45 -1.0 1,671,587 - LRK53_RS07470 0.45 +1.0 1,671,587 - LRK53_RS07470 0.45 +0.4 1,672,032 + LRK53_RS07470 0.78 +1.0 1,672,033 - LRK53_RS07470 0.78 -1.5 1,672,033 - LRK53_RS07470 0.78 +1.0 1,672,181 + LRK53_RS07470 0.89 +0.4 1,672,181 + LRK53_RS07470 0.89 +0.0 1,672,181 + LRK53_RS07470 0.89 +1.0 1,672,181 + LRK53_RS07470 0.89 +1.6 1,672,181 + LRK53_RS07470 0.89 +0.4 1,672,181 + LRK53_RS07470 0.89 +0.0 1,672,181 + LRK53_RS07470 0.89 +1.0 1,672,181 + LRK53_RS07470 0.89 +1.0 1,672,181 + LRK53_RS07470 0.89 +0.0 1,672,182 - LRK53_RS07470 0.89 -1.0 1,672,182 - LRK53_RS07470 0.89 -1.3 1,672,182 - LRK53_RS07470 0.89 -1.3 1,672,182 - LRK53_RS07470 0.89 -0.6 1,672,182 - LRK53_RS07470 0.89 -1.3 1,672,182 - LRK53_RS07470 0.89 +1.0 1,672,182 - LRK53_RS07470 0.89 +1.0 1,672,182 - LRK53_RS07470 0.89 -0.6 1,672,182 - LRK53_RS07470 0.89 +1.0 1,672,264 + -0.6 1,672,345 + -1.4 1,672,346 - -1.2 1,672,346 - -2.1 1,672,346 - +0.6 1,672,480 + LRK53_RS07475 0.56 -1.8 1,672,480 + LRK53_RS07475 0.56 -1.0 1,672,480 + LRK53_RS07475 0.56 -0.6 1,672,480 + LRK53_RS07475 0.56 -1.5 1,672,480 + LRK53_RS07475 0.56 +1.6 1,672,480 + LRK53_RS07475 0.56 -1.3 1,672,480 + LRK53_RS07475 0.56 -0.3 1,672,481 - LRK53_RS07475 0.57 -1.0 1,672,481 - LRK53_RS07475 0.57 -0.0 1,672,481 - LRK53_RS07475 0.57 +0.4 1,672,481 - LRK53_RS07475 0.57 -1.3 1,672,570 + -0.7 1,672,570 + -0.6 1,672,570 + +0.0 1,672,570 + -1.3 1,672,570 + -0.8 1,672,570 + -0.9 1,672,570 + +0.0 1,672,570 + +0.0 1,672,570 + -0.3 1,672,570 + +1.0 1,672,571 - -1.3 1,672,571 - +1.0 1,672,571 - -1.3 1,672,571 - -1.0 1,672,571 - -1.6 1,672,639 + -1.3 1,672,639 + -1.6 1,672,639 + -1.2 1,672,639 + -0.4 1,672,639 + -0.1 1,672,640 - -0.6 1,672,640 - -1.3 1,672,640 - -0.4 1,672,640 - +0.0 1,672,640 - -0.9
Or see this region's nucleotide sequence