Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07415 and LRK53_RS07420 are separated by 110 nucleotides LRK53_RS07420 and LRK53_RS07425 are separated by 32 nucleotides LRK53_RS07425 and LRK53_RS07430 are separated by 96 nucleotides
LRK53_RS07415: LRK53_RS07415 - replicative DNA helicase, at 1,659,326 to 1,660,720
_RS07415
LRK53_RS07420: LRK53_RS07420 - DNA repair protein RadA, at 1,660,831 to 1,662,216
_RS07420
LRK53_RS07425: LRK53_RS07425 - cytochrome c biogenesis protein CcsA, at 1,662,249 to 1,663,049
_RS07425
LRK53_RS07430: LRK53_RS07430 - signal recognition particle protein, at 1,663,146 to 1,664,525
_RS07430
Position (kb) 1660
1661
1662
1663 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1660.808 kb on + strand at 1660.808 kb on + strand at 1660.808 kb on + strand at 1660.808 kb on + strand at 1660.809 kb on - strand at 1660.809 kb on - strand at 1660.809 kb on - strand at 1660.809 kb on - strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.812 kb on + strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.813 kb on - strand at 1660.852 kb on + strand at 1660.853 kb on - strand at 1660.853 kb on - strand at 1660.853 kb on - strand at 1660.853 kb on - strand at 1660.853 kb on - strand at 1661.216 kb on - strand, within LRK53_RS07420 at 1661.426 kb on + strand, within LRK53_RS07420 at 1661.426 kb on + strand, within LRK53_RS07420 at 1661.427 kb on - strand, within LRK53_RS07420 at 1661.427 kb on - strand, within LRK53_RS07420 at 1661.427 kb on - strand, within LRK53_RS07420 at 1661.560 kb on + strand, within LRK53_RS07420 at 1661.560 kb on + strand, within LRK53_RS07420 at 1661.561 kb on - strand, within LRK53_RS07420 at 1661.896 kb on + strand, within LRK53_RS07420 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.382 kb on + strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1662.383 kb on - strand, within LRK53_RS07425 at 1663.009 kb on + strand at 1663.010 kb on - strand at 1663.010 kb on - strand at 1663.111 kb on + strand at 1663.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,660,808 + -0.6 1,660,808 + +0.0 1,660,808 + -0.6 1,660,808 + -0.3 1,660,809 - +0.0 1,660,809 - +0.0 1,660,809 - -0.4 1,660,809 - +0.0 1,660,812 + -0.9 1,660,812 + +0.7 1,660,812 + -0.3 1,660,812 + +0.5 1,660,812 + +2.0 1,660,812 + -0.6 1,660,812 + -1.6 1,660,812 + -1.0 1,660,812 + -1.0 1,660,812 + +0.4 1,660,812 + +0.0 1,660,813 - -1.0 1,660,813 - -2.0 1,660,813 - +1.0 1,660,813 - +1.0 1,660,813 - +1.0 1,660,813 - -0.6 1,660,813 - +0.7 1,660,813 - -1.0 1,660,852 + +1.0 1,660,853 - -1.3 1,660,853 - -1.3 1,660,853 - +0.0 1,660,853 - +0.6 1,660,853 - +1.0 1,661,216 - LRK53_RS07420 0.28 -0.6 1,661,426 + LRK53_RS07420 0.43 -1.3 1,661,426 + LRK53_RS07420 0.43 -1.6 1,661,427 - LRK53_RS07420 0.43 +0.4 1,661,427 - LRK53_RS07420 0.43 +0.0 1,661,427 - LRK53_RS07420 0.43 -0.6 1,661,560 + LRK53_RS07420 0.53 -0.6 1,661,560 + LRK53_RS07420 0.53 -1.3 1,661,561 - LRK53_RS07420 0.53 -0.6 1,661,896 + LRK53_RS07420 0.77 -1.1 1,662,382 + LRK53_RS07425 0.17 +1.0 1,662,382 + LRK53_RS07425 0.17 -0.4 1,662,382 + LRK53_RS07425 0.17 -1.5 1,662,382 + LRK53_RS07425 0.17 -0.4 1,662,383 - LRK53_RS07425 0.17 -1.3 1,662,383 - LRK53_RS07425 0.17 -0.3 1,662,383 - LRK53_RS07425 0.17 -0.1 1,662,383 - LRK53_RS07425 0.17 +0.0 1,662,383 - LRK53_RS07425 0.17 +0.0 1,663,009 + +0.0 1,663,010 - -0.9 1,663,010 - -0.8 1,663,111 + -1.1 1,663,112 - -0.3
Or see this region's nucleotide sequence