Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07390 and LRK53_RS07395 are separated by 151 nucleotides LRK53_RS07395 and LRK53_RS07400 are separated by 284 nucleotides LRK53_RS07400 and LRK53_RS07405 are separated by 14 nucleotides
LRK53_RS07390: LRK53_RS07390 - asparagine--tRNA ligase, at 1,655,588 to 1,656,988
_RS07390
LRK53_RS07395: LRK53_RS07395 - iron-sulfur cluster assembly accessory protein, at 1,657,140 to 1,657,466
_RS07395
LRK53_RS07400: LRK53_RS07400 - 30S ribosomal protein S6, at 1,657,751 to 1,658,173
_RS07400
LRK53_RS07405: LRK53_RS07405 - 30S ribosomal protein S18, at 1,658,188 to 1,658,418
_RS07405
Position (kb)
1657
1658 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1657.079 kb on + strand at 1657.079 kb on + strand at 1657.079 kb on + strand at 1657.079 kb on + strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.080 kb on - strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.084 kb on + strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.085 kb on - strand at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.257 kb on + strand, within LRK53_RS07395 at 1657.258 kb on - strand, within LRK53_RS07395 at 1657.258 kb on - strand, within LRK53_RS07395 at 1657.258 kb on - strand, within LRK53_RS07395 at 1657.258 kb on - strand, within LRK53_RS07395 at 1657.530 kb on + strand at 1657.530 kb on + strand at 1657.531 kb on - strand at 1657.531 kb on - strand at 1657.531 kb on - strand at 1657.531 kb on - strand at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.395 kb on + strand, within LRK53_RS07405 at 1658.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,657,079 + -0.4 1,657,079 + +0.0 1,657,079 + -1.3 1,657,079 + +0.7 1,657,080 - -1.2 1,657,080 - -0.8 1,657,080 - +0.3 1,657,080 - +0.4 1,657,080 - +2.0 1,657,080 - +1.0 1,657,080 - -1.0 1,657,080 - +0.0 1,657,084 + -2.2 1,657,084 + -1.0 1,657,084 + +1.0 1,657,084 + -0.6 1,657,084 + -1.6 1,657,084 + +0.9 1,657,084 + -1.3 1,657,084 + +0.4 1,657,084 + +0.4 1,657,084 + -0.6 1,657,084 + +2.0 1,657,084 + +0.1 1,657,084 + -0.6 1,657,084 + -0.3 1,657,084 + -1.0 1,657,084 + -2.0 1,657,084 + -0.2 1,657,084 + -0.1 1,657,084 + +0.0 1,657,084 + -1.9 1,657,084 + +0.0 1,657,084 + +0.0 1,657,084 + +0.2 1,657,084 + -0.3 1,657,084 + -0.8 1,657,084 + -1.2 1,657,084 + +0.0 1,657,084 + -0.6 1,657,084 + +0.0 1,657,084 + +0.4 1,657,084 + +1.0 1,657,085 - +0.4 1,657,085 - +1.0 1,657,085 - -1.3 1,657,085 - -0.3 1,657,085 - +0.0 1,657,085 - -2.3 1,657,085 - -1.0 1,657,085 - -0.6 1,657,085 - +1.6 1,657,085 - +0.0 1,657,085 - +0.8 1,657,085 - +0.8 1,657,085 - +1.6 1,657,085 - -1.3 1,657,085 - -0.3 1,657,085 - +1.0 1,657,085 - -1.8 1,657,085 - -1.0 1,657,085 - -1.0 1,657,085 - -1.0 1,657,085 - -0.8 1,657,085 - -0.6 1,657,085 - -0.8 1,657,085 - +0.0 1,657,085 - -1.0 1,657,085 - -0.6 1,657,085 - +0.0 1,657,085 - +0.0 1,657,257 + LRK53_RS07395 0.36 -0.6 1,657,257 + LRK53_RS07395 0.36 -1.6 1,657,257 + LRK53_RS07395 0.36 +0.0 1,657,257 + LRK53_RS07395 0.36 +1.6 1,657,257 + LRK53_RS07395 0.36 -2.0 1,657,257 + LRK53_RS07395 0.36 -0.2 1,657,257 + LRK53_RS07395 0.36 +0.0 1,657,258 - LRK53_RS07395 0.36 -1.6 1,657,258 - LRK53_RS07395 0.36 -3.2 1,657,258 - LRK53_RS07395 0.36 +0.2 1,657,258 - LRK53_RS07395 0.36 +1.0 1,657,530 + -0.6 1,657,530 + +0.0 1,657,531 - -3.0 1,657,531 - -0.4 1,657,531 - -2.0 1,657,531 - +0.6 1,658,395 + LRK53_RS07405 0.90 -1.3 1,658,395 + LRK53_RS07405 0.90 -1.0 1,658,395 + LRK53_RS07405 0.90 -0.6 1,658,395 + LRK53_RS07405 0.90 -0.3 1,658,395 + LRK53_RS07405 0.90 +1.0 1,658,395 + LRK53_RS07405 0.90 +0.1 1,658,395 + LRK53_RS07405 0.90 -1.0 1,658,396 - +0.0
Or see this region's nucleotide sequence