Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07355 and LRK53_RS07360 are separated by 534 nucleotides LRK53_RS07360 and LRK53_RS07365 overlap by 4 nucleotides LRK53_RS07365 and LRK53_RS07370 are separated by 30 nucleotides
LRK53_RS07355: LRK53_RS07355 - N-formylglutamate deformylase, at 1,649,618 to 1,650,400
_RS07355
LRK53_RS07360: LRK53_RS07360 - Rid family hydrolase, at 1,650,935 to 1,651,366
_RS07360
LRK53_RS07365: LRK53_RS07365 - carbonate dehydratase, at 1,651,363 to 1,652,028
_RS07365
LRK53_RS07370: LRK53_RS07370 - aldehyde dehydrogenase, at 1,652,059 to 1,653,546
_RS07370
Position (kb) 1650
1651
1652 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1649.993 kb on + strand, within LRK53_RS07355 at 1649.993 kb on + strand, within LRK53_RS07355 at 1649.993 kb on + strand, within LRK53_RS07355 at 1650.236 kb on + strand, within LRK53_RS07355 at 1650.236 kb on + strand, within LRK53_RS07355 at 1650.236 kb on + strand, within LRK53_RS07355 at 1650.237 kb on - strand, within LRK53_RS07355 at 1650.237 kb on - strand, within LRK53_RS07355 at 1650.237 kb on - strand, within LRK53_RS07355 at 1650.237 kb on - strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.308 kb on + strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.309 kb on - strand, within LRK53_RS07355 at 1650.408 kb on + strand at 1650.408 kb on + strand at 1650.408 kb on + strand at 1650.408 kb on + strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.409 kb on - strand at 1650.493 kb on + strand at 1650.493 kb on + strand at 1650.493 kb on + strand at 1650.493 kb on + strand at 1650.493 kb on + strand at 1650.493 kb on + strand at 1650.494 kb on - strand at 1650.494 kb on - strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.598 kb on + strand at 1650.599 kb on - strand at 1650.599 kb on - strand at 1650.618 kb on + strand at 1650.859 kb on + strand at 1650.860 kb on - strand at 1651.065 kb on + strand, within LRK53_RS07360 at 1651.065 kb on + strand, within LRK53_RS07360 at 1651.065 kb on + strand, within LRK53_RS07360 at 1651.065 kb on + strand, within LRK53_RS07360 at 1651.065 kb on + strand, within LRK53_RS07360 at 1651.065 kb on + strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.066 kb on - strand, within LRK53_RS07360 at 1651.197 kb on + strand, within LRK53_RS07360 at 1651.197 kb on + strand, within LRK53_RS07360 at 1651.314 kb on + strand, within LRK53_RS07360 at 1652.060 kb on + strand at 1652.060 kb on + strand at 1652.060 kb on + strand at 1652.060 kb on + strand at 1652.084 kb on + strand at 1652.084 kb on + strand at 1652.084 kb on + strand at 1652.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,649,993 + LRK53_RS07355 0.48 -0.6 1,649,993 + LRK53_RS07355 0.48 +0.0 1,649,993 + LRK53_RS07355 0.48 -0.5 1,650,236 + LRK53_RS07355 0.79 -1.6 1,650,236 + LRK53_RS07355 0.79 -2.2 1,650,236 + LRK53_RS07355 0.79 -0.8 1,650,237 - LRK53_RS07355 0.79 -1.8 1,650,237 - LRK53_RS07355 0.79 -2.0 1,650,237 - LRK53_RS07355 0.79 -0.4 1,650,237 - LRK53_RS07355 0.79 -0.6 1,650,308 + LRK53_RS07355 0.88 -0.9 1,650,308 + LRK53_RS07355 0.88 +0.1 1,650,308 + LRK53_RS07355 0.88 -0.6 1,650,308 + LRK53_RS07355 0.88 +0.3 1,650,308 + LRK53_RS07355 0.88 -0.7 1,650,308 + LRK53_RS07355 0.88 -1.0 1,650,308 + LRK53_RS07355 0.88 -1.4 1,650,308 + LRK53_RS07355 0.88 -3.4 1,650,308 + LRK53_RS07355 0.88 -0.1 1,650,308 + LRK53_RS07355 0.88 +0.0 1,650,308 + LRK53_RS07355 0.88 -0.5 1,650,308 + LRK53_RS07355 0.88 -1.2 1,650,308 + LRK53_RS07355 0.88 -2.0 1,650,308 + LRK53_RS07355 0.88 +0.0 1,650,308 + LRK53_RS07355 0.88 -1.0 1,650,308 + LRK53_RS07355 0.88 +0.0 1,650,309 - LRK53_RS07355 0.88 -0.3 1,650,309 - LRK53_RS07355 0.88 +1.0 1,650,309 - LRK53_RS07355 0.88 -0.8 1,650,309 - LRK53_RS07355 0.88 +1.0 1,650,309 - LRK53_RS07355 0.88 -0.6 1,650,309 - LRK53_RS07355 0.88 +1.0 1,650,309 - LRK53_RS07355 0.88 +0.0 1,650,309 - LRK53_RS07355 0.88 -0.6 1,650,309 - LRK53_RS07355 0.88 +0.0 1,650,408 + +0.0 1,650,408 + -0.4 1,650,408 + -1.8 1,650,408 + -0.6 1,650,409 - +1.6 1,650,409 - +0.2 1,650,409 - -0.8 1,650,409 - -0.6 1,650,409 - -2.2 1,650,409 - -1.3 1,650,409 - +2.0 1,650,409 - +0.0 1,650,493 + -1.6 1,650,493 + -1.4 1,650,493 + +0.0 1,650,493 + +2.0 1,650,493 + -1.8 1,650,493 + -1.0 1,650,494 - -0.3 1,650,494 - +1.6 1,650,598 + +0.2 1,650,598 + -0.9 1,650,598 + -0.6 1,650,598 + +0.0 1,650,598 + -1.1 1,650,598 + -0.1 1,650,598 + -1.6 1,650,598 + -0.2 1,650,599 - -0.9 1,650,599 - +0.4 1,650,618 + +0.6 1,650,859 + +0.0 1,650,860 - +1.0 1,651,065 + LRK53_RS07360 0.30 +1.6 1,651,065 + LRK53_RS07360 0.30 +0.1 1,651,065 + LRK53_RS07360 0.30 +0.0 1,651,065 + LRK53_RS07360 0.30 +1.0 1,651,065 + LRK53_RS07360 0.30 -0.7 1,651,065 + LRK53_RS07360 0.30 +1.0 1,651,066 - LRK53_RS07360 0.30 +0.4 1,651,066 - LRK53_RS07360 0.30 +0.5 1,651,066 - LRK53_RS07360 0.30 -0.6 1,651,066 - LRK53_RS07360 0.30 +0.6 1,651,066 - LRK53_RS07360 0.30 -1.0 1,651,066 - LRK53_RS07360 0.30 -0.7 1,651,066 - LRK53_RS07360 0.30 +1.0 1,651,066 - LRK53_RS07360 0.30 -1.1 1,651,197 + LRK53_RS07360 0.61 +1.0 1,651,197 + LRK53_RS07360 0.61 +0.0 1,651,314 + LRK53_RS07360 0.88 +1.2 1,652,060 + +0.0 1,652,060 + +0.3 1,652,060 + +1.0 1,652,060 + -1.9 1,652,084 + -0.6 1,652,084 + +0.0 1,652,084 + -1.6 1,652,085 - -1.3
Or see this region's nucleotide sequence