Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07035 and LRK53_RS07040 are separated by 219 nucleotides LRK53_RS07040 and LRK53_RS07045 overlap by 4 nucleotides LRK53_RS07045 and LRK53_RS07050 are separated by 237 nucleotides
LRK53_RS07035: LRK53_RS07035 - TolC family protein, at 1,582,812 to 1,584,200
_RS07035
LRK53_RS07040: LRK53_RS07040 - sensor histidine kinase, at 1,584,420 to 1,585,835
_RS07040
LRK53_RS07045: LRK53_RS07045 - response regulator, at 1,585,832 to 1,586,521
_RS07045
LRK53_RS07050: LRK53_RS07050 - sulfite reductase flavoprotein subunit alpha, at 1,586,759 to 1,588,441
_RS07050
Position (kb)
1584
1585
1586 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1583.890 kb on + strand, within LRK53_RS07035 at 1583.891 kb on - strand, within LRK53_RS07035 at 1583.891 kb on - strand, within LRK53_RS07035 at 1583.950 kb on + strand, within LRK53_RS07035 at 1583.950 kb on + strand, within LRK53_RS07035 at 1583.951 kb on - strand, within LRK53_RS07035 at 1583.951 kb on - strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.971 kb on + strand, within LRK53_RS07035 at 1583.972 kb on - strand, within LRK53_RS07035 at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.270 kb on + strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.271 kb on - strand at 1584.300 kb on - strand at 1584.339 kb on - strand at 1584.339 kb on - strand at 1585.182 kb on + strand, within LRK53_RS07040 at 1585.182 kb on + strand, within LRK53_RS07040 at 1585.183 kb on - strand, within LRK53_RS07040 at 1585.183 kb on - strand, within LRK53_RS07040 at 1585.472 kb on - strand, within LRK53_RS07040 at 1585.570 kb on + strand, within LRK53_RS07040 at 1585.571 kb on - strand, within LRK53_RS07040 at 1585.571 kb on - strand, within LRK53_RS07040 at 1585.591 kb on + strand, within LRK53_RS07040 at 1585.591 kb on + strand, within LRK53_RS07040 at 1585.592 kb on - strand, within LRK53_RS07040 at 1585.708 kb on + strand at 1585.709 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.727 kb on - strand at 1585.875 kb on + strand at 1585.876 kb on - strand at 1585.899 kb on - strand at 1586.336 kb on + strand, within LRK53_RS07045 at 1586.336 kb on + strand, within LRK53_RS07045 at 1586.337 kb on - strand, within LRK53_RS07045 at 1586.337 kb on - strand, within LRK53_RS07045 at 1586.337 kb on - strand, within LRK53_RS07045 at 1586.538 kb on + strand at 1586.538 kb on + strand at 1586.538 kb on + strand at 1586.539 kb on - strand at 1586.539 kb on - strand at 1586.539 kb on - strand at 1586.539 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.609 kb on - strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.665 kb on + strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.666 kb on - strand at 1586.695 kb on + strand at 1586.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,583,890 + LRK53_RS07035 0.78 +1.0 1,583,891 - LRK53_RS07035 0.78 +0.0 1,583,891 - LRK53_RS07035 0.78 +1.0 1,583,950 + LRK53_RS07035 0.82 +1.7 1,583,950 + LRK53_RS07035 0.82 -1.8 1,583,951 - LRK53_RS07035 0.82 +0.7 1,583,951 - LRK53_RS07035 0.82 +0.4 1,583,971 + LRK53_RS07035 0.83 -1.0 1,583,971 + LRK53_RS07035 0.83 -1.2 1,583,971 + LRK53_RS07035 0.83 -0.6 1,583,971 + LRK53_RS07035 0.83 +1.0 1,583,972 - LRK53_RS07035 0.84 +1.0 1,584,270 + -1.0 1,584,270 + +1.0 1,584,270 + -0.6 1,584,270 + +1.0 1,584,270 + +1.0 1,584,270 + +0.0 1,584,270 + +0.0 1,584,270 + -1.2 1,584,270 + +0.0 1,584,270 + -0.3 1,584,270 + -1.0 1,584,270 + +0.7 1,584,270 + +1.0 1,584,270 + +1.6 1,584,270 + +1.0 1,584,270 + +0.3 1,584,270 + -0.6 1,584,270 + +1.0 1,584,270 + +2.0 1,584,270 + -1.0 1,584,270 + -0.2 1,584,270 + +0.0 1,584,270 + +1.0 1,584,270 + -1.0 1,584,270 + -0.3 1,584,270 + +2.0 1,584,270 + -1.3 1,584,270 + +0.3 1,584,270 + +1.7 1,584,270 + +1.0 1,584,271 - -0.1 1,584,271 - -1.5 1,584,271 - -1.2 1,584,271 - -1.3 1,584,271 - -0.2 1,584,271 - +0.7 1,584,271 - +0.0 1,584,271 - +2.0 1,584,271 - -0.6 1,584,271 - -1.0 1,584,271 - +1.0 1,584,271 - -1.0 1,584,271 - -1.3 1,584,271 - -1.0 1,584,271 - -0.5 1,584,271 - +1.0 1,584,271 - -1.3 1,584,271 - -0.3 1,584,271 - +0.0 1,584,271 - -1.1 1,584,271 - -1.1 1,584,271 - -0.6 1,584,271 - -1.3 1,584,271 - +0.0 1,584,271 - +0.0 1,584,271 - +0.3 1,584,271 - +0.4 1,584,271 - +1.0 1,584,271 - -1.3 1,584,271 - +2.6 1,584,271 - -1.1 1,584,300 - -1.0 1,584,339 - -0.8 1,584,339 - +0.0 1,585,182 + LRK53_RS07040 0.54 -1.1 1,585,182 + LRK53_RS07040 0.54 -1.6 1,585,183 - LRK53_RS07040 0.54 +0.0 1,585,183 - LRK53_RS07040 0.54 -1.6 1,585,472 - LRK53_RS07040 0.74 +1.0 1,585,570 + LRK53_RS07040 0.81 +0.7 1,585,571 - LRK53_RS07040 0.81 -1.2 1,585,571 - LRK53_RS07040 0.81 +0.0 1,585,591 + LRK53_RS07040 0.83 -0.6 1,585,591 + LRK53_RS07040 0.83 +0.0 1,585,592 - LRK53_RS07040 0.83 -1.0 1,585,708 + +1.0 1,585,709 - -1.0 1,585,727 - -0.2 1,585,727 - -2.0 1,585,727 - -0.8 1,585,727 - +1.0 1,585,727 - -1.5 1,585,727 - -1.0 1,585,727 - -0.9 1,585,727 - -1.7 1,585,727 - +0.4 1,585,875 + -0.1 1,585,876 - -1.2 1,585,899 - +1.0 1,586,336 + LRK53_RS07045 0.73 +2.6 1,586,336 + LRK53_RS07045 0.73 -1.2 1,586,337 - LRK53_RS07045 0.73 +0.4 1,586,337 - LRK53_RS07045 0.73 -1.0 1,586,337 - LRK53_RS07045 0.73 +2.0 1,586,538 + -0.1 1,586,538 + -1.8 1,586,538 + +0.0 1,586,539 - -1.3 1,586,539 - -0.6 1,586,539 - -0.4 1,586,539 - -0.9 1,586,609 - -1.0 1,586,609 - -0.3 1,586,609 - -1.2 1,586,609 - -1.0 1,586,665 + -0.4 1,586,665 + -1.0 1,586,665 + -0.4 1,586,665 + +0.3 1,586,665 + -0.4 1,586,665 + +0.0 1,586,666 - +0.0 1,586,666 - +0.4 1,586,666 - -0.6 1,586,666 - +1.0 1,586,666 - -1.5 1,586,666 - -1.1 1,586,695 + -0.2 1,586,696 - +0.0
Or see this region's nucleotide sequence