Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS07010 and LRK53_RS07015 overlap by 1 nucleotides LRK53_RS07015 and LRK53_RS07020 are separated by 67 nucleotides LRK53_RS07020 and LRK53_RS07025 overlap by 4 nucleotides LRK53_RS07025 and LRK53_RS07030 are separated by 5 nucleotides
LRK53_RS07010: LRK53_RS07010 - EamA family transporter, at 1,578,541 to 1,578,921
_RS07010
LRK53_RS07015: LRK53_RS07015 - alpha/beta fold hydrolase, at 1,578,921 to 1,579,757
_RS07015
LRK53_RS07020: LRK53_RS07020 - HlyD family efflux transporter periplasmic adaptor subunit, at 1,579,825 to 1,580,793
_RS07020
LRK53_RS07025: LRK53_RS07025 - ABC transporter ATP-binding protein, at 1,580,790 to 1,581,539
_RS07025
LRK53_RS07030: LRK53_RS07030 - ABC transporter permease, at 1,581,545 to 1,582,675
_RS07030
Position (kb)
1579
1580
1581 Strain fitness (log2 ratio)
-2
-1
0
1 at 1579.027 kb on + strand, within LRK53_RS07015 at 1579.027 kb on + strand, within LRK53_RS07015 at 1579.955 kb on - strand, within LRK53_RS07020 at 1579.955 kb on - strand, within LRK53_RS07020 at 1579.988 kb on + strand, within LRK53_RS07020 at 1579.989 kb on - strand, within LRK53_RS07020 at 1579.989 kb on - strand, within LRK53_RS07020 at 1579.989 kb on - strand, within LRK53_RS07020 at 1580.847 kb on + strand at 1580.847 kb on + strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.848 kb on - strand at 1580.924 kb on + strand, within LRK53_RS07025 at 1580.924 kb on + strand, within LRK53_RS07025 at 1580.937 kb on - strand, within LRK53_RS07025 at 1581.547 kb on - strand at 1581.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,579,027 + LRK53_RS07015 0.13 -0.6 1,579,027 + LRK53_RS07015 0.13 -1.0 1,579,955 - LRK53_RS07020 0.13 -1.3 1,579,955 - LRK53_RS07020 0.13 -0.6 1,579,988 + LRK53_RS07020 0.17 -1.0 1,579,989 - LRK53_RS07020 0.17 -2.2 1,579,989 - LRK53_RS07020 0.17 -0.6 1,579,989 - LRK53_RS07020 0.17 +0.0 1,580,847 + -0.6 1,580,847 + +0.0 1,580,848 - -0.6 1,580,848 - +1.0 1,580,848 - +0.0 1,580,848 - +0.5 1,580,848 - +1.0 1,580,848 - -0.8 1,580,848 - -0.8 1,580,848 - -2.0 1,580,924 + LRK53_RS07025 0.18 -1.3 1,580,924 + LRK53_RS07025 0.18 +0.7 1,580,937 - LRK53_RS07025 0.20 -1.3 1,581,547 - -1.0 1,581,547 - -0.7
Or see this region's nucleotide sequence