Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06935 and LRK53_RS06940 overlap by 4 nucleotides LRK53_RS06940 and LRK53_RS06945 are separated by 320 nucleotides LRK53_RS06945 and LRK53_RS06950 overlap by 1 nucleotides LRK53_RS06950 and LRK53_RS06955 are separated by 91 nucleotides
LRK53_RS06935: LRK53_RS06935 - type II toxin-antitoxin system VapB family antitoxin, at 1,565,928 to 1,566,143
_RS06935
LRK53_RS06940: LRK53_RS06940 - PIN domain nuclease, at 1,566,140 to 1,566,532
_RS06940
LRK53_RS06945: LRK53_RS06945 - beta-ketoacyl-[acyl-carrier-protein] synthase family protein, at 1,566,853 to 1,568,085
_RS06945
LRK53_RS06950: LRK53_RS06950 - phosphopantetheine-binding protein, at 1,568,085 to 1,568,357
_RS06950
LRK53_RS06955: LRK53_RS06955 - GNAT family N-acetyltransferase, at 1,568,449 to 1,569,519
_RS06955
Position (kb)
1566
1567
1568
1569 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.855 kb on + strand at 1565.856 kb on - strand at 1565.927 kb on + strand at 1565.928 kb on - strand at 1565.928 kb on - strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.944 kb on + strand at 1565.960 kb on + strand, within LRK53_RS06935 at 1565.960 kb on + strand, within LRK53_RS06935 at 1566.173 kb on + strand at 1566.173 kb on + strand at 1566.173 kb on + strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.174 kb on - strand at 1566.544 kb on + strand at 1566.544 kb on + strand at 1566.544 kb on + strand at 1566.545 kb on - strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.550 kb on + strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.551 kb on - strand at 1566.684 kb on + strand at 1566.684 kb on + strand at 1566.685 kb on - strand at 1566.685 kb on - strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.748 kb on + strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.749 kb on - strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.760 kb on + strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.761 kb on - strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.956 kb on + strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1566.957 kb on - strand at 1567.196 kb on + strand, within LRK53_RS06945 at 1567.196 kb on + strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.197 kb on - strand, within LRK53_RS06945 at 1567.482 kb on - strand, within LRK53_RS06945 at 1567.482 kb on - strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.598 kb on + strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.599 kb on - strand, within LRK53_RS06945 at 1567.915 kb on + strand, within LRK53_RS06945 at 1567.916 kb on - strand, within LRK53_RS06945 at 1568.430 kb on + strand at 1568.430 kb on + strand at 1568.431 kb on - strand at 1568.431 kb on - strand at 1568.444 kb on + strand at 1568.444 kb on + strand at 1568.444 kb on + strand at 1568.444 kb on + strand at 1568.445 kb on - strand at 1568.445 kb on - strand at 1568.445 kb on - strand at 1568.445 kb on - strand at 1568.626 kb on + strand, within LRK53_RS06955 at 1568.690 kb on - strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.929 kb on + strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1568.930 kb on - strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.082 kb on + strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955 at 1569.083 kb on - strand, within LRK53_RS06955
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,565,855 + -0.9 1,565,855 + -0.4 1,565,855 + -0.6 1,565,855 + +2.4 1,565,855 + +0.6 1,565,855 + -1.2 1,565,856 - +2.0 1,565,927 + +0.3 1,565,928 - -1.3 1,565,928 - +1.6 1,565,944 + +1.0 1,565,944 + -1.1 1,565,944 + -1.6 1,565,944 + +2.0 1,565,960 + LRK53_RS06935 0.15 +1.0 1,565,960 + LRK53_RS06935 0.15 +1.0 1,566,173 + +1.0 1,566,173 + -0.6 1,566,173 + +1.0 1,566,174 - -0.7 1,566,174 - +0.0 1,566,174 - +0.5 1,566,174 - +1.7 1,566,544 + +2.0 1,566,544 + +0.0 1,566,544 + -2.7 1,566,545 - +1.0 1,566,550 + +0.0 1,566,550 + +1.0 1,566,550 + -1.3 1,566,550 + -0.2 1,566,550 + -1.0 1,566,551 - +0.4 1,566,551 - +0.4 1,566,551 - +1.0 1,566,551 - -1.7 1,566,551 - -1.2 1,566,551 - +0.7 1,566,551 - -1.0 1,566,551 - -0.9 1,566,551 - +0.0 1,566,684 + -0.6 1,566,684 + -1.6 1,566,685 - -0.6 1,566,685 - +0.3 1,566,748 + +1.0 1,566,748 + +0.0 1,566,748 + +0.3 1,566,748 + +0.8 1,566,748 + -0.2 1,566,748 + -0.6 1,566,748 + +0.4 1,566,748 + -1.5 1,566,748 + -0.2 1,566,748 + -0.5 1,566,748 + -0.8 1,566,748 + -1.7 1,566,748 + -1.3 1,566,748 + -1.6 1,566,748 + -0.6 1,566,748 + -0.3 1,566,749 - -0.6 1,566,749 - -0.6 1,566,749 - -1.0 1,566,749 - +0.4 1,566,749 - -0.6 1,566,749 - +1.0 1,566,749 - -0.4 1,566,749 - -1.3 1,566,749 - -0.8 1,566,749 - -0.4 1,566,749 - +0.0 1,566,749 - +0.0 1,566,760 + -1.3 1,566,760 + -1.0 1,566,760 + -1.3 1,566,760 + -0.4 1,566,760 + -0.3 1,566,760 + -0.6 1,566,760 + -1.2 1,566,760 + -2.3 1,566,760 + -0.5 1,566,760 + -0.6 1,566,760 + -3.6 1,566,760 + -0.6 1,566,760 + -1.0 1,566,761 - -1.0 1,566,761 - +1.0 1,566,761 - +0.6 1,566,761 - -2.5 1,566,761 - -1.0 1,566,761 - -0.6 1,566,761 - +0.3 1,566,761 - -0.8 1,566,956 + -0.6 1,566,956 + -0.6 1,566,956 + +1.0 1,566,956 + +1.0 1,566,956 + -0.9 1,566,956 + +1.0 1,566,956 + -0.6 1,566,956 + +1.0 1,566,957 - +2.6 1,566,957 - -0.6 1,566,957 - -0.8 1,566,957 - -0.6 1,566,957 - +1.6 1,566,957 - -1.8 1,566,957 - +2.6 1,567,196 + LRK53_RS06945 0.28 -1.0 1,567,196 + LRK53_RS06945 0.28 -1.2 1,567,197 - LRK53_RS06945 0.28 -1.7 1,567,197 - LRK53_RS06945 0.28 -1.6 1,567,197 - LRK53_RS06945 0.28 +0.4 1,567,197 - LRK53_RS06945 0.28 +0.0 1,567,197 - LRK53_RS06945 0.28 -1.8 1,567,197 - LRK53_RS06945 0.28 -1.0 1,567,197 - LRK53_RS06945 0.28 -2.2 1,567,197 - LRK53_RS06945 0.28 +0.0 1,567,197 - LRK53_RS06945 0.28 +1.0 1,567,197 - LRK53_RS06945 0.28 -0.6 1,567,197 - LRK53_RS06945 0.28 -0.6 1,567,482 - LRK53_RS06945 0.51 +0.0 1,567,482 - LRK53_RS06945 0.51 -0.3 1,567,598 + LRK53_RS06945 0.60 +0.0 1,567,598 + LRK53_RS06945 0.60 -1.3 1,567,598 + LRK53_RS06945 0.60 +2.0 1,567,598 + LRK53_RS06945 0.60 -0.6 1,567,598 + LRK53_RS06945 0.60 -1.8 1,567,599 - LRK53_RS06945 0.61 +0.0 1,567,599 - LRK53_RS06945 0.61 -1.0 1,567,599 - LRK53_RS06945 0.61 +0.0 1,567,599 - LRK53_RS06945 0.61 -2.0 1,567,599 - LRK53_RS06945 0.61 -2.5 1,567,599 - LRK53_RS06945 0.61 -0.6 1,567,599 - LRK53_RS06945 0.61 -2.2 1,567,599 - LRK53_RS06945 0.61 +0.0 1,567,599 - LRK53_RS06945 0.61 +0.3 1,567,915 + LRK53_RS06945 0.86 -1.9 1,567,916 - LRK53_RS06945 0.86 +1.0 1,568,430 + -0.6 1,568,430 + +1.0 1,568,431 - +0.2 1,568,431 - -1.0 1,568,444 + +0.0 1,568,444 + -1.0 1,568,444 + -0.4 1,568,444 + -0.6 1,568,445 - +0.1 1,568,445 - -0.5 1,568,445 - -2.6 1,568,445 - -0.9 1,568,626 + LRK53_RS06955 0.17 -0.3 1,568,690 - LRK53_RS06955 0.23 +0.3 1,568,929 + LRK53_RS06955 0.45 -1.7 1,568,929 + LRK53_RS06955 0.45 -0.5 1,568,929 + LRK53_RS06955 0.45 -0.6 1,568,929 + LRK53_RS06955 0.45 -1.0 1,568,929 + LRK53_RS06955 0.45 +0.2 1,568,929 + LRK53_RS06955 0.45 -1.1 1,568,929 + LRK53_RS06955 0.45 -1.0 1,568,929 + LRK53_RS06955 0.45 -0.7 1,568,929 + LRK53_RS06955 0.45 -0.2 1,568,929 + LRK53_RS06955 0.45 +0.1 1,568,930 - LRK53_RS06955 0.45 +0.0 1,568,930 - LRK53_RS06955 0.45 +0.7 1,568,930 - LRK53_RS06955 0.45 +0.0 1,568,930 - LRK53_RS06955 0.45 -0.6 1,568,930 - LRK53_RS06955 0.45 -0.6 1,568,930 - LRK53_RS06955 0.45 -0.4 1,569,082 + LRK53_RS06955 0.59 +1.0 1,569,082 + LRK53_RS06955 0.59 -1.7 1,569,082 + LRK53_RS06955 0.59 -2.0 1,569,082 + LRK53_RS06955 0.59 -1.1 1,569,082 + LRK53_RS06955 0.59 -1.0 1,569,082 + LRK53_RS06955 0.59 -1.6 1,569,082 + LRK53_RS06955 0.59 +1.0 1,569,082 + LRK53_RS06955 0.59 -1.0 1,569,083 - LRK53_RS06955 0.59 +0.0 1,569,083 - LRK53_RS06955 0.59 -1.5 1,569,083 - LRK53_RS06955 0.59 +1.0 1,569,083 - LRK53_RS06955 0.59 -1.3 1,569,083 - LRK53_RS06955 0.59 -1.3 1,569,083 - LRK53_RS06955 0.59 +0.0 1,569,083 - LRK53_RS06955 0.59 -0.4 1,569,083 - LRK53_RS06955 0.59 -0.6 1,569,083 - LRK53_RS06955 0.59 -0.6 1,569,083 - LRK53_RS06955 0.59 -0.6
Or see this region's nucleotide sequence