Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06810 and LRK53_RS06815 are separated by 111 nucleotides LRK53_RS06815 and LRK53_RS06820 are separated by 144 nucleotides LRK53_RS06820 and LRK53_RS06825 are separated by 366 nucleotides
LRK53_RS06810: LRK53_RS06810 - uracil phosphoribosyltransferase, at 1,539,369 to 1,540,013
_RS06810
LRK53_RS06815: LRK53_RS06815 - PsiF family protein, at 1,540,125 to 1,540,490
_RS06815
LRK53_RS06820: LRK53_RS06820 - aminotransferase class III-fold pyridoxal phosphate-dependent enzyme, at 1,540,635 to 1,542,146
_RS06820
LRK53_RS06825: LRK53_RS06825 - ABC transporter ATP-binding protein, at 1,542,513 to 1,543,135
_RS06825
Position (kb) 1540
1541
1542
1543 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.673 kb on + strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.674 kb on - strand, within LRK53_RS06810 at 1539.676 kb on + strand, within LRK53_RS06810 at 1539.676 kb on + strand, within LRK53_RS06810 at 1539.676 kb on + strand, within LRK53_RS06810 at 1539.676 kb on + strand, within LRK53_RS06810 at 1539.676 kb on + strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.677 kb on - strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.892 kb on + strand, within LRK53_RS06810 at 1539.893 kb on - strand, within LRK53_RS06810 at 1539.893 kb on - strand, within LRK53_RS06810 at 1539.893 kb on - strand, within LRK53_RS06810 at 1539.893 kb on - strand, within LRK53_RS06810 at 1539.893 kb on - strand, within LRK53_RS06810 at 1540.061 kb on - strand at 1540.061 kb on - strand at 1540.061 kb on - strand at 1540.126 kb on + strand at 1540.126 kb on + strand at 1540.126 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.505 kb on + strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.506 kb on - strand at 1540.537 kb on + strand at 1540.537 kb on + strand at 1540.537 kb on + strand at 1540.537 kb on + strand at 1540.538 kb on - strand at 1540.538 kb on - strand at 1540.538 kb on - strand at 1540.538 kb on - strand at 1540.538 kb on - strand at 1540.538 kb on - strand at 1540.589 kb on + strand at 1540.589 kb on + strand at 1540.589 kb on + strand at 1540.589 kb on + strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.590 kb on - strand at 1540.795 kb on + strand, within LRK53_RS06820 at 1540.795 kb on + strand, within LRK53_RS06820 at 1540.796 kb on - strand, within LRK53_RS06820 at 1540.796 kb on - strand, within LRK53_RS06820 at 1541.267 kb on - strand, within LRK53_RS06820 at 1541.267 kb on - strand, within LRK53_RS06820 at 1541.450 kb on - strand, within LRK53_RS06820 at 1541.647 kb on + strand, within LRK53_RS06820 at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.204 kb on - strand at 1542.280 kb on + strand at 1542.280 kb on + strand at 1542.281 kb on - strand at 1542.281 kb on - strand at 1542.281 kb on - strand at 1542.449 kb on + strand at 1542.450 kb on - strand at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.792 kb on + strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.793 kb on - strand, within LRK53_RS06825 at 1542.876 kb on - strand, within LRK53_RS06825 at 1543.046 kb on + strand, within LRK53_RS06825 at 1543.046 kb on + strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825 at 1543.047 kb on - strand, within LRK53_RS06825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,539,673 + LRK53_RS06810 0.47 -0.7 1,539,673 + LRK53_RS06810 0.47 -1.3 1,539,673 + LRK53_RS06810 0.47 -0.3 1,539,673 + LRK53_RS06810 0.47 -0.8 1,539,673 + LRK53_RS06810 0.47 -2.2 1,539,673 + LRK53_RS06810 0.47 -0.8 1,539,673 + LRK53_RS06810 0.47 -0.8 1,539,673 + LRK53_RS06810 0.47 -0.0 1,539,674 - LRK53_RS06810 0.47 +1.0 1,539,674 - LRK53_RS06810 0.47 -1.3 1,539,674 - LRK53_RS06810 0.47 -1.0 1,539,674 - LRK53_RS06810 0.47 -2.3 1,539,674 - LRK53_RS06810 0.47 -0.6 1,539,674 - LRK53_RS06810 0.47 -0.3 1,539,674 - LRK53_RS06810 0.47 -0.0 1,539,674 - LRK53_RS06810 0.47 -1.5 1,539,674 - LRK53_RS06810 0.47 +2.0 1,539,676 + LRK53_RS06810 0.48 +0.6 1,539,676 + LRK53_RS06810 0.48 -2.2 1,539,676 + LRK53_RS06810 0.48 -0.0 1,539,676 + LRK53_RS06810 0.48 -0.6 1,539,676 + LRK53_RS06810 0.48 +1.0 1,539,677 - LRK53_RS06810 0.48 +0.6 1,539,677 - LRK53_RS06810 0.48 -0.0 1,539,677 - LRK53_RS06810 0.48 -1.3 1,539,677 - LRK53_RS06810 0.48 -0.6 1,539,677 - LRK53_RS06810 0.48 -1.2 1,539,677 - LRK53_RS06810 0.48 -1.1 1,539,677 - LRK53_RS06810 0.48 -0.6 1,539,677 - LRK53_RS06810 0.48 -2.1 1,539,677 - LRK53_RS06810 0.48 -0.2 1,539,677 - LRK53_RS06810 0.48 -0.0 1,539,677 - LRK53_RS06810 0.48 -0.7 1,539,677 - LRK53_RS06810 0.48 -0.6 1,539,677 - LRK53_RS06810 0.48 -0.0 1,539,677 - LRK53_RS06810 0.48 -0.6 1,539,677 - LRK53_RS06810 0.48 -0.0 1,539,677 - LRK53_RS06810 0.48 +0.4 1,539,892 + LRK53_RS06810 0.81 +0.3 1,539,892 + LRK53_RS06810 0.81 -0.1 1,539,892 + LRK53_RS06810 0.81 -2.0 1,539,892 + LRK53_RS06810 0.81 +2.0 1,539,892 + LRK53_RS06810 0.81 -3.9 1,539,892 + LRK53_RS06810 0.81 -1.6 1,539,892 + LRK53_RS06810 0.81 +0.3 1,539,892 + LRK53_RS06810 0.81 -1.9 1,539,892 + LRK53_RS06810 0.81 -0.0 1,539,892 + LRK53_RS06810 0.81 -0.8 1,539,892 + LRK53_RS06810 0.81 -1.2 1,539,892 + LRK53_RS06810 0.81 -0.6 1,539,892 + LRK53_RS06810 0.81 -0.9 1,539,893 - LRK53_RS06810 0.81 +0.6 1,539,893 - LRK53_RS06810 0.81 -0.0 1,539,893 - LRK53_RS06810 0.81 -0.0 1,539,893 - LRK53_RS06810 0.81 +1.6 1,539,893 - LRK53_RS06810 0.81 -1.3 1,540,061 - -0.0 1,540,061 - +1.0 1,540,061 - -1.0 1,540,126 + -0.6 1,540,126 + -0.6 1,540,126 + +0.4 1,540,505 + +0.7 1,540,505 + -1.6 1,540,505 + +1.0 1,540,505 + -1.3 1,540,505 + -1.3 1,540,505 + +1.0 1,540,505 + +1.0 1,540,505 + -0.4 1,540,506 - -0.1 1,540,506 - -1.2 1,540,506 - -3.6 1,540,506 - -1.0 1,540,506 - -0.6 1,540,506 - -0.2 1,540,506 - -0.3 1,540,506 - -1.6 1,540,506 - -1.6 1,540,506 - -1.2 1,540,506 - -0.9 1,540,506 - -0.6 1,540,506 - +0.1 1,540,506 - +0.4 1,540,537 + -0.3 1,540,537 + +0.4 1,540,537 + -1.6 1,540,537 + -0.6 1,540,538 - -2.0 1,540,538 - -0.0 1,540,538 - -1.0 1,540,538 - -1.3 1,540,538 - -1.3 1,540,538 - -0.2 1,540,589 + +0.4 1,540,589 + -0.0 1,540,589 + -1.3 1,540,589 + -0.4 1,540,590 - -0.9 1,540,590 - +0.4 1,540,590 - -0.5 1,540,590 - -0.6 1,540,590 - -0.3 1,540,590 - -1.2 1,540,590 - -0.0 1,540,590 - +1.0 1,540,590 - -0.2 1,540,590 - -1.0 1,540,590 - -0.3 1,540,590 - +1.0 1,540,590 - -1.2 1,540,590 - -1.1 1,540,590 - +0.4 1,540,795 + LRK53_RS06820 0.11 -1.0 1,540,795 + LRK53_RS06820 0.11 -4.5 1,540,796 - LRK53_RS06820 0.11 -1.0 1,540,796 - LRK53_RS06820 0.11 -2.9 1,541,267 - LRK53_RS06820 0.42 -1.0 1,541,267 - LRK53_RS06820 0.42 -2.5 1,541,450 - LRK53_RS06820 0.54 -0.6 1,541,647 + LRK53_RS06820 0.67 -1.0 1,542,204 - -1.3 1,542,204 - +1.6 1,542,204 - -0.0 1,542,204 - -0.0 1,542,204 - +1.0 1,542,204 - -0.6 1,542,204 - -0.6 1,542,204 - +2.0 1,542,204 - +0.3 1,542,204 - -0.3 1,542,204 - +2.0 1,542,204 - -1.0 1,542,204 - -0.0 1,542,204 - +2.0 1,542,204 - -1.0 1,542,204 - -0.8 1,542,204 - -0.3 1,542,204 - -0.3 1,542,204 - +0.8 1,542,280 + +2.0 1,542,280 + -1.3 1,542,281 - -0.2 1,542,281 - -1.3 1,542,281 - +1.0 1,542,449 + -1.3 1,542,450 - +0.3 1,542,792 + LRK53_RS06825 0.45 -1.0 1,542,792 + LRK53_RS06825 0.45 -0.9 1,542,792 + LRK53_RS06825 0.45 -0.1 1,542,792 + LRK53_RS06825 0.45 +0.4 1,542,792 + LRK53_RS06825 0.45 -1.7 1,542,792 + LRK53_RS06825 0.45 +0.3 1,542,792 + LRK53_RS06825 0.45 +1.0 1,542,792 + LRK53_RS06825 0.45 -0.6 1,542,792 + LRK53_RS06825 0.45 -0.0 1,542,792 + LRK53_RS06825 0.45 +2.6 1,542,792 + LRK53_RS06825 0.45 +2.0 1,542,792 + LRK53_RS06825 0.45 -0.6 1,542,792 + LRK53_RS06825 0.45 -1.1 1,542,792 + LRK53_RS06825 0.45 -0.0 1,542,792 + LRK53_RS06825 0.45 -0.0 1,542,792 + LRK53_RS06825 0.45 -2.0 1,542,792 + LRK53_RS06825 0.45 -0.6 1,542,792 + LRK53_RS06825 0.45 -1.6 1,542,792 + LRK53_RS06825 0.45 -2.3 1,542,792 + LRK53_RS06825 0.45 -0.9 1,542,792 + LRK53_RS06825 0.45 -0.6 1,542,793 - LRK53_RS06825 0.45 -0.7 1,542,793 - LRK53_RS06825 0.45 +0.7 1,542,793 - LRK53_RS06825 0.45 -1.0 1,542,793 - LRK53_RS06825 0.45 +1.0 1,542,793 - LRK53_RS06825 0.45 -1.4 1,542,793 - LRK53_RS06825 0.45 -0.1 1,542,793 - LRK53_RS06825 0.45 -0.0 1,542,793 - LRK53_RS06825 0.45 -0.0 1,542,793 - LRK53_RS06825 0.45 +1.0 1,542,793 - LRK53_RS06825 0.45 -0.4 1,542,793 - LRK53_RS06825 0.45 -1.0 1,542,793 - LRK53_RS06825 0.45 -0.6 1,542,793 - LRK53_RS06825 0.45 +0.8 1,542,793 - LRK53_RS06825 0.45 +0.4 1,542,793 - LRK53_RS06825 0.45 -2.3 1,542,793 - LRK53_RS06825 0.45 +0.1 1,542,793 - LRK53_RS06825 0.45 -1.5 1,542,793 - LRK53_RS06825 0.45 +2.0 1,542,793 - LRK53_RS06825 0.45 -0.6 1,542,793 - LRK53_RS06825 0.45 +1.0 1,542,793 - LRK53_RS06825 0.45 -0.7 1,542,793 - LRK53_RS06825 0.45 +1.6 1,542,876 - LRK53_RS06825 0.58 +0.4 1,543,046 + LRK53_RS06825 0.86 -0.6 1,543,046 + LRK53_RS06825 0.86 -0.6 1,543,047 - LRK53_RS06825 0.86 -0.3 1,543,047 - LRK53_RS06825 0.86 -0.3 1,543,047 - LRK53_RS06825 0.86 +1.6 1,543,047 - LRK53_RS06825 0.86 -1.0 1,543,047 - LRK53_RS06825 0.86 -0.0 1,543,047 - LRK53_RS06825 0.86 -0.0 1,543,047 - LRK53_RS06825 0.86 -0.0 1,543,047 - LRK53_RS06825 0.86 +1.3 1,543,047 - LRK53_RS06825 0.86 -0.0
Or see this region's nucleotide sequence