Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06565 and LRK53_RS06570 are separated by 725 nucleotides LRK53_RS06570 and LRK53_RS06575 are separated by 28 nucleotides LRK53_RS06575 and LRK53_RS06580 are separated by 100 nucleotides
LRK53_RS06565: LRK53_RS06565 - metalloprotease TldD, at 1,488,540 to 1,489,979
_RS06565
LRK53_RS06570: LRK53_RS06570 - DUF615 domain-containing protein, at 1,490,705 to 1,491,262
_RS06570
LRK53_RS06575: LRK53_RS06575 - DUF1653 domain-containing protein, at 1,491,291 to 1,491,542
_RS06575
LRK53_RS06580: LRK53_RS06580 - metalloprotease PmbA, at 1,491,643 to 1,493,010
_RS06580
Position (kb)
1490
1491
1492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1489.761 kb on + strand, within LRK53_RS06565 at 1489.761 kb on + strand, within LRK53_RS06565 at 1489.761 kb on + strand, within LRK53_RS06565 at 1489.762 kb on - strand, within LRK53_RS06565 at 1489.762 kb on - strand, within LRK53_RS06565 at 1489.762 kb on - strand, within LRK53_RS06565 at 1489.762 kb on - strand, within LRK53_RS06565 at 1489.960 kb on + strand at 1489.960 kb on + strand at 1489.960 kb on + strand at 1489.960 kb on + strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.961 kb on - strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.977 kb on + strand at 1489.978 kb on - strand at 1489.978 kb on - strand at 1489.978 kb on - strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.995 kb on + strand at 1489.996 kb on - strand at 1489.996 kb on - strand at 1489.996 kb on - strand at 1489.996 kb on - strand at 1489.996 kb on - strand at 1489.996 kb on - strand at 1490.565 kb on + strand at 1490.565 kb on + strand at 1490.566 kb on - strand at 1490.566 kb on - strand at 1490.571 kb on + strand at 1490.571 kb on + strand at 1490.571 kb on + strand at 1490.571 kb on + strand at 1490.571 kb on + strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.572 kb on - strand at 1490.574 kb on + strand at 1490.574 kb on + strand at 1490.575 kb on - strand at 1490.575 kb on - strand at 1490.575 kb on - strand at 1490.605 kb on + strand at 1490.606 kb on - strand at 1490.696 kb on + strand at 1490.696 kb on + strand at 1490.696 kb on + strand at 1490.697 kb on - strand at 1490.697 kb on - strand at 1490.697 kb on - strand at 1490.766 kb on + strand, within LRK53_RS06570 at 1490.817 kb on - strand, within LRK53_RS06570 at 1491.431 kb on - strand, within LRK53_RS06575 at 1491.431 kb on - strand, within LRK53_RS06575 at 1491.442 kb on + strand, within LRK53_RS06575 at 1491.442 kb on + strand, within LRK53_RS06575 at 1491.443 kb on - strand, within LRK53_RS06575 at 1491.443 kb on - strand, within LRK53_RS06575 at 1491.443 kb on - strand, within LRK53_RS06575 at 1491.443 kb on - strand, within LRK53_RS06575 at 1491.483 kb on - strand, within LRK53_RS06575 at 1491.483 kb on - strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.511 kb on + strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.512 kb on - strand, within LRK53_RS06575 at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.520 kb on + strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.521 kb on - strand at 1491.533 kb on + strand at 1491.533 kb on + strand at 1491.533 kb on + strand at 1491.533 kb on + strand at 1491.533 kb on + strand at 1491.534 kb on - strand at 1491.534 kb on - strand at 1491.608 kb on + strand at 1491.608 kb on + strand at 1491.609 kb on - strand at 1491.609 kb on - strand at 1491.609 kb on - strand at 1491.613 kb on + strand at 1491.614 kb on - strand at 1491.614 kb on - strand at 1491.614 kb on - strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.621 kb on + strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.622 kb on - strand at 1491.623 kb on + strand at 1491.856 kb on + strand, within LRK53_RS06580 at 1491.856 kb on + strand, within LRK53_RS06580 at 1491.856 kb on + strand, within LRK53_RS06580 at 1491.856 kb on + strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.857 kb on - strand, within LRK53_RS06580 at 1491.949 kb on + strand, within LRK53_RS06580 at 1492.156 kb on + strand, within LRK53_RS06580 at 1492.156 kb on + strand, within LRK53_RS06580 at 1492.156 kb on + strand, within LRK53_RS06580 at 1492.255 kb on + strand, within LRK53_RS06580 at 1492.255 kb on + strand, within LRK53_RS06580 at 1492.255 kb on + strand, within LRK53_RS06580 at 1492.255 kb on + strand, within LRK53_RS06580 at 1492.256 kb on - strand, within LRK53_RS06580 at 1492.256 kb on - strand, within LRK53_RS06580 at 1492.261 kb on + strand, within LRK53_RS06580 at 1492.261 kb on + strand, within LRK53_RS06580 at 1492.262 kb on - strand, within LRK53_RS06580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,489,761 + LRK53_RS06565 0.85 +0.0 1,489,761 + LRK53_RS06565 0.85 -0.8 1,489,761 + LRK53_RS06565 0.85 -0.5 1,489,762 - LRK53_RS06565 0.85 -1.3 1,489,762 - LRK53_RS06565 0.85 -0.6 1,489,762 - LRK53_RS06565 0.85 -0.9 1,489,762 - LRK53_RS06565 0.85 +1.0 1,489,960 + -0.4 1,489,960 + +0.0 1,489,960 + -1.3 1,489,960 + -0.6 1,489,961 - -0.6 1,489,961 - +1.0 1,489,961 - -2.0 1,489,961 - -1.3 1,489,961 - -1.5 1,489,961 - -1.3 1,489,961 - -1.3 1,489,961 - -0.6 1,489,961 - +0.0 1,489,961 - +1.0 1,489,977 + +0.3 1,489,977 + -0.6 1,489,977 + -0.6 1,489,977 + -1.0 1,489,977 + -1.0 1,489,977 + -1.2 1,489,977 + -0.6 1,489,977 + +0.0 1,489,977 + +2.2 1,489,977 + -0.5 1,489,977 + -0.7 1,489,978 - -0.2 1,489,978 - -1.6 1,489,978 - +0.0 1,489,995 + -0.6 1,489,995 + +0.4 1,489,995 + -0.7 1,489,995 + -0.8 1,489,995 + -1.6 1,489,995 + -0.4 1,489,995 + +1.0 1,489,995 + -0.6 1,489,996 - -0.6 1,489,996 - -0.4 1,489,996 - +0.0 1,489,996 - -0.4 1,489,996 - +1.0 1,489,996 - -0.3 1,490,565 + -0.3 1,490,565 + +0.3 1,490,566 - +0.3 1,490,566 - -1.0 1,490,571 + +0.1 1,490,571 + -0.6 1,490,571 + -1.0 1,490,571 + -0.3 1,490,571 + -1.3 1,490,572 - -1.3 1,490,572 - +0.4 1,490,572 - +1.3 1,490,572 - +0.0 1,490,572 - -1.2 1,490,572 - +0.4 1,490,572 - +0.7 1,490,572 - +0.5 1,490,572 - +1.0 1,490,574 + -1.4 1,490,574 + -0.3 1,490,575 - +1.0 1,490,575 - -0.9 1,490,575 - -1.3 1,490,605 + -0.3 1,490,606 - -1.3 1,490,696 + -0.8 1,490,696 + -1.0 1,490,696 + -1.6 1,490,697 - -1.3 1,490,697 - +0.7 1,490,697 - -0.6 1,490,766 + LRK53_RS06570 0.11 -1.0 1,490,817 - LRK53_RS06570 0.20 -0.9 1,491,431 - LRK53_RS06575 0.56 -0.2 1,491,431 - LRK53_RS06575 0.56 +0.3 1,491,442 + LRK53_RS06575 0.60 -2.9 1,491,442 + LRK53_RS06575 0.60 -2.7 1,491,443 - LRK53_RS06575 0.60 +2.0 1,491,443 - LRK53_RS06575 0.60 -3.0 1,491,443 - LRK53_RS06575 0.60 +0.0 1,491,443 - LRK53_RS06575 0.60 -0.3 1,491,483 - LRK53_RS06575 0.76 -0.9 1,491,483 - LRK53_RS06575 0.76 -0.6 1,491,511 + LRK53_RS06575 0.87 -0.3 1,491,511 + LRK53_RS06575 0.87 -1.0 1,491,511 + LRK53_RS06575 0.87 +1.0 1,491,511 + LRK53_RS06575 0.87 -1.0 1,491,511 + LRK53_RS06575 0.87 -0.6 1,491,511 + LRK53_RS06575 0.87 -0.9 1,491,511 + LRK53_RS06575 0.87 -2.2 1,491,511 + LRK53_RS06575 0.87 -1.4 1,491,512 - LRK53_RS06575 0.88 -1.2 1,491,512 - LRK53_RS06575 0.88 +0.4 1,491,512 - LRK53_RS06575 0.88 -1.4 1,491,512 - LRK53_RS06575 0.88 +0.0 1,491,512 - LRK53_RS06575 0.88 +0.4 1,491,512 - LRK53_RS06575 0.88 -0.6 1,491,512 - LRK53_RS06575 0.88 -0.2 1,491,520 + +1.0 1,491,520 + -0.6 1,491,520 + -0.2 1,491,520 + +2.0 1,491,520 + -1.0 1,491,520 + +0.2 1,491,520 + -1.3 1,491,520 + +0.0 1,491,520 + -0.1 1,491,520 + -1.0 1,491,520 + +0.0 1,491,520 + +0.0 1,491,520 + -1.0 1,491,520 + +0.0 1,491,520 + -0.6 1,491,520 + -1.5 1,491,521 - -2.3 1,491,521 - -0.6 1,491,521 - +1.0 1,491,521 - +0.0 1,491,521 - +1.3 1,491,521 - -0.6 1,491,521 - +1.0 1,491,521 - -0.6 1,491,521 - +1.0 1,491,521 - -1.2 1,491,521 - +0.3 1,491,521 - -1.0 1,491,521 - -0.3 1,491,521 - -1.3 1,491,521 - -0.3 1,491,521 - -0.3 1,491,521 - -0.3 1,491,521 - +1.0 1,491,521 - +0.0 1,491,521 - +0.6 1,491,521 - -1.4 1,491,521 - -1.1 1,491,521 - -0.7 1,491,521 - -2.2 1,491,521 - +1.0 1,491,533 + -1.0 1,491,533 + -0.1 1,491,533 + -1.8 1,491,533 + +0.0 1,491,533 + -0.1 1,491,534 - -1.3 1,491,534 - -1.6 1,491,608 + +0.0 1,491,608 + +0.0 1,491,609 - +0.3 1,491,609 - -0.3 1,491,609 - +1.0 1,491,613 + +1.3 1,491,614 - -0.6 1,491,614 - +1.0 1,491,614 - -0.9 1,491,621 + -1.8 1,491,621 + -0.6 1,491,621 + +1.0 1,491,621 + +0.6 1,491,621 + -2.1 1,491,621 + -0.5 1,491,621 + +1.0 1,491,621 + +0.0 1,491,621 + -1.0 1,491,621 + -1.4 1,491,621 + -1.8 1,491,621 + +0.4 1,491,621 + +0.0 1,491,621 + +2.0 1,491,621 + -0.8 1,491,621 + -2.3 1,491,621 + -0.6 1,491,621 + +0.4 1,491,621 + -1.3 1,491,622 - +1.0 1,491,622 - -0.8 1,491,622 - +0.0 1,491,622 - +0.0 1,491,622 - +2.0 1,491,622 - +0.4 1,491,622 - -1.1 1,491,622 - +1.0 1,491,622 - -0.6 1,491,622 - -0.7 1,491,622 - -0.5 1,491,622 - +1.0 1,491,622 - -1.3 1,491,622 - +1.0 1,491,623 + -0.8 1,491,856 + LRK53_RS06580 0.16 +1.0 1,491,856 + LRK53_RS06580 0.16 +2.0 1,491,856 + LRK53_RS06580 0.16 -1.6 1,491,856 + LRK53_RS06580 0.16 -2.2 1,491,857 - LRK53_RS06580 0.16 -3.7 1,491,857 - LRK53_RS06580 0.16 -0.8 1,491,857 - LRK53_RS06580 0.16 -0.8 1,491,857 - LRK53_RS06580 0.16 +0.4 1,491,857 - LRK53_RS06580 0.16 -0.6 1,491,857 - LRK53_RS06580 0.16 +1.0 1,491,857 - LRK53_RS06580 0.16 +2.0 1,491,949 + LRK53_RS06580 0.22 +0.2 1,492,156 + LRK53_RS06580 0.38 +0.0 1,492,156 + LRK53_RS06580 0.38 +1.0 1,492,156 + LRK53_RS06580 0.38 -1.0 1,492,255 + LRK53_RS06580 0.45 +1.6 1,492,255 + LRK53_RS06580 0.45 +1.0 1,492,255 + LRK53_RS06580 0.45 +2.0 1,492,255 + LRK53_RS06580 0.45 -1.7 1,492,256 - LRK53_RS06580 0.45 +0.0 1,492,256 - LRK53_RS06580 0.45 -0.9 1,492,261 + LRK53_RS06580 0.45 -0.7 1,492,261 + LRK53_RS06580 0.45 +0.0 1,492,262 - LRK53_RS06580 0.45 -1.4
Or see this region's nucleotide sequence