Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS06540 and LRK53_RS06545 are separated by 36 nucleotides LRK53_RS06545 and LRK53_RS06550 are separated by 67 nucleotides LRK53_RS06550 and LRK53_RS06555 are separated by 169 nucleotides
LRK53_RS06540: LRK53_RS06540 - ribonuclease G, at 1,479,535 to 1,481,022
_RS06540
LRK53_RS06545: LRK53_RS06545 - TIGR02099 family protein, at 1,481,059 to 1,484,997
_RS06545
LRK53_RS06550: LRK53_RS06550 - hypothetical protein, at 1,485,065 to 1,485,715
_RS06550
LRK53_RS06555: LRK53_RS06555 - tetratricopeptide repeat protein, at 1,485,885 to 1,488,140
_RS06555
Position (kb)
1481
1482
1483
1484
1485 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1480.114 kb on + strand, within LRK53_RS06540 at 1480.114 kb on + strand, within LRK53_RS06540 at 1480.114 kb on + strand, within LRK53_RS06540 at 1480.114 kb on + strand, within LRK53_RS06540 at 1480.114 kb on + strand, within LRK53_RS06540 at 1480.114 kb on + strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.115 kb on - strand, within LRK53_RS06540 at 1480.180 kb on + strand, within LRK53_RS06540 at 1480.180 kb on + strand, within LRK53_RS06540 at 1480.181 kb on - strand, within LRK53_RS06540 at 1480.181 kb on - strand, within LRK53_RS06540 at 1480.330 kb on + strand, within LRK53_RS06540 at 1480.330 kb on + strand, within LRK53_RS06540 at 1480.330 kb on + strand, within LRK53_RS06540 at 1480.331 kb on - strand, within LRK53_RS06540 at 1480.331 kb on - strand, within LRK53_RS06540 at 1480.331 kb on - strand, within LRK53_RS06540 at 1480.331 kb on - strand, within LRK53_RS06540 at 1480.361 kb on - strand, within LRK53_RS06540 at 1480.361 kb on - strand, within LRK53_RS06540 at 1480.361 kb on - strand, within LRK53_RS06540 at 1480.423 kb on + strand, within LRK53_RS06540 at 1480.480 kb on + strand, within LRK53_RS06540 at 1480.480 kb on + strand, within LRK53_RS06540 at 1480.481 kb on - strand, within LRK53_RS06540 at 1480.481 kb on - strand, within LRK53_RS06540 at 1480.513 kb on + strand, within LRK53_RS06540 at 1480.684 kb on + strand, within LRK53_RS06540 at 1480.684 kb on + strand, within LRK53_RS06540 at 1480.684 kb on + strand, within LRK53_RS06540 at 1480.684 kb on + strand, within LRK53_RS06540 at 1480.816 kb on + strand, within LRK53_RS06540 at 1480.816 kb on + strand, within LRK53_RS06540 at 1480.816 kb on + strand, within LRK53_RS06540 at 1480.817 kb on - strand, within LRK53_RS06540 at 1480.817 kb on - strand, within LRK53_RS06540 at 1480.817 kb on - strand, within LRK53_RS06540 at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.964 kb on + strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.965 kb on - strand at 1480.987 kb on + strand at 1480.987 kb on + strand at 1480.987 kb on + strand at 1480.988 kb on - strand at 1480.988 kb on - strand at 1480.988 kb on - strand at 1481.020 kb on + strand at 1481.020 kb on + strand at 1481.020 kb on + strand at 1481.020 kb on + strand at 1481.020 kb on + strand at 1481.020 kb on + strand at 1481.021 kb on - strand at 1481.332 kb on - strand at 1481.587 kb on + strand, within LRK53_RS06545 at 1481.587 kb on + strand, within LRK53_RS06545 at 1481.588 kb on - strand, within LRK53_RS06545 at 1481.969 kb on - strand, within LRK53_RS06545 at 1482.430 kb on - strand, within LRK53_RS06545 at 1482.533 kb on - strand, within LRK53_RS06545 at 1482.533 kb on - strand, within LRK53_RS06545 at 1483.294 kb on + strand, within LRK53_RS06545 at 1483.453 kb on - strand, within LRK53_RS06545 at 1483.579 kb on + strand, within LRK53_RS06545 at 1483.580 kb on - strand, within LRK53_RS06545 at 1483.717 kb on + strand, within LRK53_RS06545 at 1483.717 kb on + strand, within LRK53_RS06545 at 1483.717 kb on + strand, within LRK53_RS06545 at 1483.717 kb on + strand, within LRK53_RS06545 at 1483.717 kb on + strand, within LRK53_RS06545 at 1483.717 kb on + strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.718 kb on - strand, within LRK53_RS06545 at 1483.883 kb on - strand, within LRK53_RS06545 at 1483.883 kb on - strand, within LRK53_RS06545 at 1483.883 kb on - strand, within LRK53_RS06545 at 1483.883 kb on - strand, within LRK53_RS06545 at 1483.883 kb on - strand, within LRK53_RS06545 at 1483.883 kb on - strand, within LRK53_RS06545 at 1483.942 kb on + strand, within LRK53_RS06545 at 1483.942 kb on + strand, within LRK53_RS06545 at 1483.943 kb on - strand, within LRK53_RS06545 at 1483.943 kb on - strand, within LRK53_RS06545 at 1484.681 kb on - strand at 1484.996 kb on - strand at 1485.069 kb on + strand at 1485.069 kb on + strand at 1485.070 kb on - strand at 1485.070 kb on - strand at 1485.105 kb on + strand at 1485.105 kb on + strand at 1485.105 kb on + strand at 1485.105 kb on + strand at 1485.106 kb on - strand at 1485.106 kb on - strand at 1485.106 kb on - strand at 1485.106 kb on - strand at 1485.106 kb on - strand at 1485.106 kb on - strand at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.255 kb on + strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.256 kb on - strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.369 kb on + strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550 at 1485.370 kb on - strand, within LRK53_RS06550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,480,114 + LRK53_RS06540 0.39 +1.0 1,480,114 + LRK53_RS06540 0.39 -1.0 1,480,114 + LRK53_RS06540 0.39 -1.2 1,480,114 + LRK53_RS06540 0.39 +0.4 1,480,114 + LRK53_RS06540 0.39 +1.0 1,480,114 + LRK53_RS06540 0.39 -1.0 1,480,115 - LRK53_RS06540 0.39 -0.2 1,480,115 - LRK53_RS06540 0.39 +0.5 1,480,115 - LRK53_RS06540 0.39 +2.0 1,480,115 - LRK53_RS06540 0.39 -0.6 1,480,115 - LRK53_RS06540 0.39 +0.0 1,480,115 - LRK53_RS06540 0.39 +0.0 1,480,115 - LRK53_RS06540 0.39 +0.6 1,480,115 - LRK53_RS06540 0.39 +0.3 1,480,115 - LRK53_RS06540 0.39 +2.0 1,480,115 - LRK53_RS06540 0.39 +0.0 1,480,180 + LRK53_RS06540 0.43 +0.0 1,480,180 + LRK53_RS06540 0.43 -0.6 1,480,181 - LRK53_RS06540 0.43 -1.3 1,480,181 - LRK53_RS06540 0.43 -0.6 1,480,330 + LRK53_RS06540 0.53 +0.7 1,480,330 + LRK53_RS06540 0.53 -0.3 1,480,330 + LRK53_RS06540 0.53 -1.6 1,480,331 - LRK53_RS06540 0.53 +1.0 1,480,331 - LRK53_RS06540 0.53 -1.6 1,480,331 - LRK53_RS06540 0.53 -0.4 1,480,331 - LRK53_RS06540 0.53 -2.8 1,480,361 - LRK53_RS06540 0.56 -0.5 1,480,361 - LRK53_RS06540 0.56 +0.0 1,480,361 - LRK53_RS06540 0.56 +1.0 1,480,423 + LRK53_RS06540 0.60 -1.0 1,480,480 + LRK53_RS06540 0.64 -0.6 1,480,480 + LRK53_RS06540 0.64 -2.5 1,480,481 - LRK53_RS06540 0.64 +0.0 1,480,481 - LRK53_RS06540 0.64 -1.4 1,480,513 + LRK53_RS06540 0.66 -2.0 1,480,684 + LRK53_RS06540 0.77 -0.2 1,480,684 + LRK53_RS06540 0.77 -1.3 1,480,684 + LRK53_RS06540 0.77 -1.6 1,480,684 + LRK53_RS06540 0.77 -1.2 1,480,816 + LRK53_RS06540 0.86 -0.6 1,480,816 + LRK53_RS06540 0.86 +1.7 1,480,816 + LRK53_RS06540 0.86 +0.4 1,480,817 - LRK53_RS06540 0.86 -0.4 1,480,817 - LRK53_RS06540 0.86 -1.3 1,480,817 - LRK53_RS06540 0.86 -1.0 1,480,964 + -0.6 1,480,964 + -0.6 1,480,964 + -1.0 1,480,964 + -0.1 1,480,964 + +0.3 1,480,964 + +1.0 1,480,964 + +1.0 1,480,964 + -2.6 1,480,965 - +1.2 1,480,965 - +0.1 1,480,965 - +0.0 1,480,965 - +2.0 1,480,965 - -0.6 1,480,965 - +1.0 1,480,965 - -0.4 1,480,965 - -1.3 1,480,965 - -2.3 1,480,965 - -0.6 1,480,965 - +0.0 1,480,965 - -0.4 1,480,965 - -2.3 1,480,965 - +0.0 1,480,965 - +1.0 1,480,965 - -0.6 1,480,965 - +1.0 1,480,965 - -1.3 1,480,987 + +1.0 1,480,987 + -0.9 1,480,987 + +1.0 1,480,988 - +0.0 1,480,988 - -2.2 1,480,988 - +0.3 1,481,020 + -0.3 1,481,020 + -0.8 1,481,020 + -0.2 1,481,020 + -1.8 1,481,020 + +0.0 1,481,020 + -1.6 1,481,021 - +0.0 1,481,332 - -1.0 1,481,587 + LRK53_RS06545 0.13 +0.4 1,481,587 + LRK53_RS06545 0.13 +1.0 1,481,588 - LRK53_RS06545 0.13 +1.0 1,481,969 - LRK53_RS06545 0.23 +0.0 1,482,430 - LRK53_RS06545 0.35 +0.0 1,482,533 - LRK53_RS06545 0.37 +1.0 1,482,533 - LRK53_RS06545 0.37 +1.0 1,483,294 + LRK53_RS06545 0.57 +2.0 1,483,453 - LRK53_RS06545 0.61 -0.6 1,483,579 + LRK53_RS06545 0.64 +1.0 1,483,580 - LRK53_RS06545 0.64 +1.0 1,483,717 + LRK53_RS06545 0.67 +1.0 1,483,717 + LRK53_RS06545 0.67 +0.0 1,483,717 + LRK53_RS06545 0.67 -0.6 1,483,717 + LRK53_RS06545 0.67 +2.0 1,483,717 + LRK53_RS06545 0.67 +0.0 1,483,717 + LRK53_RS06545 0.67 +1.0 1,483,718 - LRK53_RS06545 0.68 -1.0 1,483,718 - LRK53_RS06545 0.68 +1.0 1,483,718 - LRK53_RS06545 0.68 -0.8 1,483,718 - LRK53_RS06545 0.68 +1.0 1,483,718 - LRK53_RS06545 0.68 -0.6 1,483,718 - LRK53_RS06545 0.68 +1.0 1,483,718 - LRK53_RS06545 0.68 +1.0 1,483,883 - LRK53_RS06545 0.72 -0.6 1,483,883 - LRK53_RS06545 0.72 -0.6 1,483,883 - LRK53_RS06545 0.72 +0.0 1,483,883 - LRK53_RS06545 0.72 -1.3 1,483,883 - LRK53_RS06545 0.72 +0.0 1,483,883 - LRK53_RS06545 0.72 +1.0 1,483,942 + LRK53_RS06545 0.73 -1.3 1,483,942 + LRK53_RS06545 0.73 -1.3 1,483,943 - LRK53_RS06545 0.73 -0.6 1,483,943 - LRK53_RS06545 0.73 +0.0 1,484,681 - -1.8 1,484,996 - -1.0 1,485,069 + +0.0 1,485,069 + +0.3 1,485,070 - -1.3 1,485,070 - -1.3 1,485,105 + +1.0 1,485,105 + +0.0 1,485,105 + +0.4 1,485,105 + -1.0 1,485,106 - +1.0 1,485,106 - +1.0 1,485,106 - -0.5 1,485,106 - -0.3 1,485,106 - -0.6 1,485,106 - +0.2 1,485,255 + LRK53_RS06550 0.29 -0.8 1,485,255 + LRK53_RS06550 0.29 -0.6 1,485,255 + LRK53_RS06550 0.29 -1.0 1,485,255 + LRK53_RS06550 0.29 -0.6 1,485,255 + LRK53_RS06550 0.29 -1.3 1,485,255 + LRK53_RS06550 0.29 -2.2 1,485,255 + LRK53_RS06550 0.29 -1.1 1,485,255 + LRK53_RS06550 0.29 +3.0 1,485,255 + LRK53_RS06550 0.29 +1.0 1,485,255 + LRK53_RS06550 0.29 -0.5 1,485,255 + LRK53_RS06550 0.29 +1.0 1,485,255 + LRK53_RS06550 0.29 -1.3 1,485,256 - LRK53_RS06550 0.29 -1.0 1,485,256 - LRK53_RS06550 0.29 -0.6 1,485,256 - LRK53_RS06550 0.29 -1.3 1,485,256 - LRK53_RS06550 0.29 +0.0 1,485,256 - LRK53_RS06550 0.29 -0.4 1,485,256 - LRK53_RS06550 0.29 -0.8 1,485,256 - LRK53_RS06550 0.29 -1.0 1,485,256 - LRK53_RS06550 0.29 -0.6 1,485,256 - LRK53_RS06550 0.29 -0.8 1,485,369 + LRK53_RS06550 0.47 -1.3 1,485,369 + LRK53_RS06550 0.47 +0.0 1,485,369 + LRK53_RS06550 0.47 +0.4 1,485,369 + LRK53_RS06550 0.47 +0.0 1,485,369 + LRK53_RS06550 0.47 -0.6 1,485,369 + LRK53_RS06550 0.47 +0.6 1,485,369 + LRK53_RS06550 0.47 +0.0 1,485,369 + LRK53_RS06550 0.47 +0.4 1,485,369 + LRK53_RS06550 0.47 -0.6 1,485,369 + LRK53_RS06550 0.47 -0.2 1,485,369 + LRK53_RS06550 0.47 -1.0 1,485,369 + LRK53_RS06550 0.47 +1.0 1,485,369 + LRK53_RS06550 0.47 -0.6 1,485,370 - LRK53_RS06550 0.47 -0.3 1,485,370 - LRK53_RS06550 0.47 +2.0 1,485,370 - LRK53_RS06550 0.47 -0.6 1,485,370 - LRK53_RS06550 0.47 -0.6 1,485,370 - LRK53_RS06550 0.47 +0.0 1,485,370 - LRK53_RS06550 0.47 +0.0 1,485,370 - LRK53_RS06550 0.47 +1.6 1,485,370 - LRK53_RS06550 0.47 +1.0 1,485,370 - LRK53_RS06550 0.47 -1.1 1,485,370 - LRK53_RS06550 0.47 +0.0 1,485,370 - LRK53_RS06550 0.47 +0.6
Or see this region's nucleotide sequence