Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05855 and LRK53_RS05860 are separated by 138 nucleotides LRK53_RS05860 and LRK53_RS05865 are separated by 286 nucleotides LRK53_RS05865 and LRK53_RS05870 are separated by 70 nucleotides
LRK53_RS05855: LRK53_RS05855 - isoaspartyl peptidase/L-asparaginase, at 1,357,796 to 1,358,791
_RS05855
LRK53_RS05860: LRK53_RS05860 - hypothetical protein, at 1,358,930 to 1,359,253
_RS05860
LRK53_RS05865: LRK53_RS05865 - response regulator transcription factor, at 1,359,540 to 1,360,127
_RS05865
LRK53_RS05870: LRK53_RS05870 - ATP-binding protein, at 1,360,198 to 1,361,472
_RS05870
Position (kb)
1359
1360
1361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.681 kb on + strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.682 kb on - strand, within LRK53_RS05855 at 1358.727 kb on - strand at 1358.804 kb on - strand at 1358.804 kb on - strand at 1358.804 kb on - strand at 1358.804 kb on - strand at 1358.810 kb on - strand at 1358.810 kb on - strand at 1358.810 kb on - strand at 1358.810 kb on - strand at 1358.931 kb on + strand at 1358.931 kb on + strand at 1358.931 kb on + strand at 1358.931 kb on + strand at 1358.932 kb on - strand at 1358.986 kb on + strand, within LRK53_RS05860 at 1358.987 kb on - strand, within LRK53_RS05860 at 1359.132 kb on + strand, within LRK53_RS05860 at 1359.132 kb on + strand, within LRK53_RS05860 at 1359.350 kb on - strand at 1359.350 kb on - strand at 1359.382 kb on + strand at 1359.382 kb on + strand at 1359.382 kb on + strand at 1359.383 kb on - strand at 1359.396 kb on + strand at 1359.397 kb on - strand at 1359.397 kb on - strand at 1359.480 kb on - strand at 1359.480 kb on - strand at 1359.480 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.486 kb on - strand at 1359.524 kb on + strand at 1359.524 kb on + strand at 1359.524 kb on + strand at 1359.805 kb on + strand, within LRK53_RS05865 at 1359.805 kb on + strand, within LRK53_RS05865 at 1359.806 kb on - strand, within LRK53_RS05865 at 1359.806 kb on - strand, within LRK53_RS05865 at 1359.806 kb on - strand, within LRK53_RS05865 at 1359.806 kb on - strand, within LRK53_RS05865 at 1359.806 kb on - strand, within LRK53_RS05865 at 1359.806 kb on - strand, within LRK53_RS05865 at 1359.856 kb on + strand, within LRK53_RS05865 at 1359.873 kb on + strand, within LRK53_RS05865 at 1359.873 kb on + strand, within LRK53_RS05865 at 1359.874 kb on - strand, within LRK53_RS05865 at 1359.874 kb on - strand, within LRK53_RS05865 at 1359.874 kb on - strand, within LRK53_RS05865 at 1359.874 kb on - strand, within LRK53_RS05865 at 1359.874 kb on - strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.879 kb on + strand, within LRK53_RS05865 at 1359.880 kb on - strand, within LRK53_RS05865 at 1359.880 kb on - strand, within LRK53_RS05865 at 1359.880 kb on - strand, within LRK53_RS05865 at 1359.880 kb on - strand, within LRK53_RS05865 at 1359.958 kb on + strand, within LRK53_RS05865 at 1359.958 kb on + strand, within LRK53_RS05865 at 1359.958 kb on + strand, within LRK53_RS05865 at 1359.958 kb on + strand, within LRK53_RS05865 at 1360.199 kb on + strand at 1360.200 kb on - strand at 1360.258 kb on - strand at 1360.258 kb on - strand at 1360.258 kb on - strand at 1360.258 kb on - strand at 1360.258 kb on - strand at 1360.258 kb on - strand at 1360.296 kb on + strand at 1360.296 kb on + strand at 1360.296 kb on + strand at 1360.297 kb on - strand at 1360.297 kb on - strand at 1360.297 kb on - strand at 1360.744 kb on - strand, within LRK53_RS05870 at 1360.744 kb on - strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.051 kb on + strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.052 kb on - strand, within LRK53_RS05870 at 1361.057 kb on + strand, within LRK53_RS05870 at 1361.057 kb on + strand, within LRK53_RS05870 at 1361.057 kb on + strand, within LRK53_RS05870 at 1361.057 kb on + strand, within LRK53_RS05870 at 1361.057 kb on + strand, within LRK53_RS05870 at 1361.057 kb on + strand, within LRK53_RS05870 at 1361.058 kb on - strand, within LRK53_RS05870 at 1361.058 kb on - strand, within LRK53_RS05870 at 1361.058 kb on - strand, within LRK53_RS05870 at 1361.058 kb on - strand, within LRK53_RS05870 at 1361.058 kb on - strand, within LRK53_RS05870 at 1361.058 kb on - strand, within LRK53_RS05870 at 1361.072 kb on + strand, within LRK53_RS05870 at 1361.072 kb on + strand, within LRK53_RS05870 at 1361.072 kb on + strand, within LRK53_RS05870 at 1361.073 kb on - strand, within LRK53_RS05870 at 1361.073 kb on - strand, within LRK53_RS05870 at 1361.073 kb on - strand, within LRK53_RS05870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,358,681 + LRK53_RS05855 0.89 -0.8 1,358,681 + LRK53_RS05855 0.89 -0.4 1,358,681 + LRK53_RS05855 0.89 -0.1 1,358,681 + LRK53_RS05855 0.89 -1.0 1,358,681 + LRK53_RS05855 0.89 -1.6 1,358,681 + LRK53_RS05855 0.89 -0.6 1,358,681 + LRK53_RS05855 0.89 -0.8 1,358,681 + LRK53_RS05855 0.89 -1.5 1,358,682 - LRK53_RS05855 0.89 -0.6 1,358,682 - LRK53_RS05855 0.89 -0.9 1,358,682 - LRK53_RS05855 0.89 +1.0 1,358,682 - LRK53_RS05855 0.89 -0.0 1,358,682 - LRK53_RS05855 0.89 +0.4 1,358,682 - LRK53_RS05855 0.89 -0.6 1,358,682 - LRK53_RS05855 0.89 -0.5 1,358,682 - LRK53_RS05855 0.89 -1.3 1,358,682 - LRK53_RS05855 0.89 -1.1 1,358,682 - LRK53_RS05855 0.89 +1.0 1,358,682 - LRK53_RS05855 0.89 -1.0 1,358,682 - LRK53_RS05855 0.89 -1.8 1,358,682 - LRK53_RS05855 0.89 -0.0 1,358,727 - -0.5 1,358,804 - +0.3 1,358,804 - -0.5 1,358,804 - -1.8 1,358,804 - -0.3 1,358,810 - -1.0 1,358,810 - -0.6 1,358,810 - -0.9 1,358,810 - -1.3 1,358,931 + -0.7 1,358,931 + -0.0 1,358,931 + -1.6 1,358,931 + +0.4 1,358,932 - -0.1 1,358,986 + LRK53_RS05860 0.17 -1.4 1,358,987 - LRK53_RS05860 0.18 -0.0 1,359,132 + LRK53_RS05860 0.62 +1.0 1,359,132 + LRK53_RS05860 0.62 -0.9 1,359,350 - -0.4 1,359,350 - -0.0 1,359,382 + +0.6 1,359,382 + -0.0 1,359,382 + -1.8 1,359,383 - -0.3 1,359,396 + -0.5 1,359,397 - -0.8 1,359,397 - +0.3 1,359,480 - -0.0 1,359,480 - -1.0 1,359,480 - +0.4 1,359,486 - +1.0 1,359,486 - -1.3 1,359,486 - -0.6 1,359,486 - -1.3 1,359,486 - -0.3 1,359,486 - -0.3 1,359,486 - +0.3 1,359,486 - -0.0 1,359,524 + -0.6 1,359,524 + +0.6 1,359,524 + -0.5 1,359,805 + LRK53_RS05865 0.45 +2.0 1,359,805 + LRK53_RS05865 0.45 -0.7 1,359,806 - LRK53_RS05865 0.45 -0.0 1,359,806 - LRK53_RS05865 0.45 -3.1 1,359,806 - LRK53_RS05865 0.45 -0.0 1,359,806 - LRK53_RS05865 0.45 -0.6 1,359,806 - LRK53_RS05865 0.45 -1.3 1,359,806 - LRK53_RS05865 0.45 +1.5 1,359,856 + LRK53_RS05865 0.54 -1.6 1,359,873 + LRK53_RS05865 0.57 -0.0 1,359,873 + LRK53_RS05865 0.57 -0.6 1,359,874 - LRK53_RS05865 0.57 -1.6 1,359,874 - LRK53_RS05865 0.57 -1.6 1,359,874 - LRK53_RS05865 0.57 +1.6 1,359,874 - LRK53_RS05865 0.57 -1.8 1,359,874 - LRK53_RS05865 0.57 +1.0 1,359,879 + LRK53_RS05865 0.58 -0.6 1,359,879 + LRK53_RS05865 0.58 -0.6 1,359,879 + LRK53_RS05865 0.58 -1.5 1,359,879 + LRK53_RS05865 0.58 -2.2 1,359,879 + LRK53_RS05865 0.58 +1.0 1,359,879 + LRK53_RS05865 0.58 -1.6 1,359,879 + LRK53_RS05865 0.58 -0.6 1,359,879 + LRK53_RS05865 0.58 -0.6 1,359,879 + LRK53_RS05865 0.58 -2.3 1,359,879 + LRK53_RS05865 0.58 -1.6 1,359,880 - LRK53_RS05865 0.58 -0.6 1,359,880 - LRK53_RS05865 0.58 -0.6 1,359,880 - LRK53_RS05865 0.58 -1.0 1,359,880 - LRK53_RS05865 0.58 -1.3 1,359,958 + LRK53_RS05865 0.71 +1.0 1,359,958 + LRK53_RS05865 0.71 -1.6 1,359,958 + LRK53_RS05865 0.71 +1.0 1,359,958 + LRK53_RS05865 0.71 -0.6 1,360,199 + -0.0 1,360,200 - -1.3 1,360,258 - +0.4 1,360,258 - -0.0 1,360,258 - -2.5 1,360,258 - -1.0 1,360,258 - +1.3 1,360,258 - -1.0 1,360,296 + -0.3 1,360,296 + -0.0 1,360,296 + -1.3 1,360,297 - -1.0 1,360,297 - -0.2 1,360,297 - -0.9 1,360,744 - LRK53_RS05870 0.43 -0.6 1,360,744 - LRK53_RS05870 0.43 +1.0 1,361,051 + LRK53_RS05870 0.67 +0.6 1,361,051 + LRK53_RS05870 0.67 -2.3 1,361,051 + LRK53_RS05870 0.67 -1.3 1,361,051 + LRK53_RS05870 0.67 -1.8 1,361,051 + LRK53_RS05870 0.67 -1.1 1,361,051 + LRK53_RS05870 0.67 -2.8 1,361,051 + LRK53_RS05870 0.67 -1.3 1,361,051 + LRK53_RS05870 0.67 -0.9 1,361,051 + LRK53_RS05870 0.67 -0.3 1,361,051 + LRK53_RS05870 0.67 -0.4 1,361,051 + LRK53_RS05870 0.67 -1.1 1,361,051 + LRK53_RS05870 0.67 -0.0 1,361,051 + LRK53_RS05870 0.67 -0.7 1,361,051 + LRK53_RS05870 0.67 -1.3 1,361,051 + LRK53_RS05870 0.67 -1.4 1,361,051 + LRK53_RS05870 0.67 -1.0 1,361,052 - LRK53_RS05870 0.67 -0.8 1,361,052 - LRK53_RS05870 0.67 -1.8 1,361,052 - LRK53_RS05870 0.67 -0.0 1,361,052 - LRK53_RS05870 0.67 -1.3 1,361,052 - LRK53_RS05870 0.67 -0.8 1,361,052 - LRK53_RS05870 0.67 -0.0 1,361,052 - LRK53_RS05870 0.67 -2.9 1,361,052 - LRK53_RS05870 0.67 -1.8 1,361,052 - LRK53_RS05870 0.67 -0.7 1,361,052 - LRK53_RS05870 0.67 -1.0 1,361,052 - LRK53_RS05870 0.67 +1.0 1,361,052 - LRK53_RS05870 0.67 -0.1 1,361,052 - LRK53_RS05870 0.67 +1.0 1,361,052 - LRK53_RS05870 0.67 -0.4 1,361,052 - LRK53_RS05870 0.67 -1.6 1,361,052 - LRK53_RS05870 0.67 -1.0 1,361,052 - LRK53_RS05870 0.67 -0.8 1,361,057 + LRK53_RS05870 0.67 -2.3 1,361,057 + LRK53_RS05870 0.67 -1.3 1,361,057 + LRK53_RS05870 0.67 -0.0 1,361,057 + LRK53_RS05870 0.67 -1.7 1,361,057 + LRK53_RS05870 0.67 -0.7 1,361,057 + LRK53_RS05870 0.67 -0.0 1,361,058 - LRK53_RS05870 0.67 -0.0 1,361,058 - LRK53_RS05870 0.67 +1.0 1,361,058 - LRK53_RS05870 0.67 -0.6 1,361,058 - LRK53_RS05870 0.67 -0.0 1,361,058 - LRK53_RS05870 0.67 -1.1 1,361,058 - LRK53_RS05870 0.67 -1.3 1,361,072 + LRK53_RS05870 0.69 -1.0 1,361,072 + LRK53_RS05870 0.69 -2.9 1,361,072 + LRK53_RS05870 0.69 -1.7 1,361,073 - LRK53_RS05870 0.69 -0.4 1,361,073 - LRK53_RS05870 0.69 -1.0 1,361,073 - LRK53_RS05870 0.69 -1.3
Or see this region's nucleotide sequence