Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05100 and LRK53_RS05105 are separated by 49 nucleotides LRK53_RS05105 and LRK53_RS05110 are separated by 98 nucleotides LRK53_RS05110 and LRK53_RS05115 are separated by 139 nucleotides LRK53_RS05115 and LRK53_RS05120 are separated by 34 nucleotides
LRK53_RS05100: LRK53_RS05100 - aspartate--tRNA ligase, at 1,200,937 to 1,202,700
_RS05100
LRK53_RS05105: LRK53_RS05105 - alpha/beta hydrolase, at 1,202,750 to 1,203,421
_RS05105
LRK53_RS05110: LRK53_RS05110 - YebC/PmpR family DNA-binding transcriptional regulator, at 1,203,520 to 1,204,248
_RS05110
LRK53_RS05115: LRK53_RS05115 - crossover junction endodeoxyribonuclease RuvC, at 1,204,388 to 1,204,906
_RS05115
LRK53_RS05120: LRK53_RS05120 - Holliday junction branch migration protein RuvA, at 1,204,941 to 1,205,537
_RS05120
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1202.772 kb on - strand at 1202.772 kb on - strand at 1202.894 kb on + strand, within LRK53_RS05105 at 1202.894 kb on + strand, within LRK53_RS05105 at 1202.894 kb on + strand, within LRK53_RS05105 at 1202.895 kb on - strand, within LRK53_RS05105 at 1202.895 kb on - strand, within LRK53_RS05105 at 1202.947 kb on + strand, within LRK53_RS05105 at 1202.947 kb on + strand, within LRK53_RS05105 at 1202.947 kb on + strand, within LRK53_RS05105 at 1202.947 kb on + strand, within LRK53_RS05105 at 1202.948 kb on - strand, within LRK53_RS05105 at 1202.948 kb on - strand, within LRK53_RS05105 at 1203.337 kb on + strand, within LRK53_RS05105 at 1203.337 kb on + strand, within LRK53_RS05105 at 1203.338 kb on - strand, within LRK53_RS05105 at 1203.526 kb on - strand at 1203.606 kb on + strand, within LRK53_RS05110 at 1203.606 kb on + strand, within LRK53_RS05110 at 1203.607 kb on - strand, within LRK53_RS05110 at 1203.607 kb on - strand, within LRK53_RS05110 at 1203.770 kb on - strand, within LRK53_RS05110 at 1203.770 kb on - strand, within LRK53_RS05110 at 1203.854 kb on - strand, within LRK53_RS05110 at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.323 kb on + strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand at 1204.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 2 mM remove 1,202,772 - -0.0 1,202,772 - -0.6 1,202,894 + LRK53_RS05105 0.21 -0.6 1,202,894 + LRK53_RS05105 0.21 -2.4 1,202,894 + LRK53_RS05105 0.21 -1.4 1,202,895 - LRK53_RS05105 0.22 -0.9 1,202,895 - LRK53_RS05105 0.22 +1.0 1,202,947 + LRK53_RS05105 0.29 -0.3 1,202,947 + LRK53_RS05105 0.29 -1.4 1,202,947 + LRK53_RS05105 0.29 +1.0 1,202,947 + LRK53_RS05105 0.29 -0.3 1,202,948 - LRK53_RS05105 0.29 -1.5 1,202,948 - LRK53_RS05105 0.29 -0.2 1,203,337 + LRK53_RS05105 0.87 -0.6 1,203,337 + LRK53_RS05105 0.87 +1.0 1,203,338 - LRK53_RS05105 0.88 -1.6 1,203,526 - -2.4 1,203,606 + LRK53_RS05110 0.12 -1.6 1,203,606 + LRK53_RS05110 0.12 -1.1 1,203,607 - LRK53_RS05110 0.12 -1.2 1,203,607 - LRK53_RS05110 0.12 -1.2 1,203,770 - LRK53_RS05110 0.34 -0.8 1,203,770 - LRK53_RS05110 0.34 -1.0 1,203,854 - LRK53_RS05110 0.46 -2.2 1,204,323 + -0.0 1,204,323 + +2.6 1,204,323 + -0.0 1,204,323 + -0.4 1,204,323 + -1.2 1,204,323 + -2.4 1,204,323 + -0.7 1,204,323 + +1.0 1,204,324 - -0.9 1,204,324 - -0.0 1,204,324 - -1.4 1,204,324 - -0.6 1,204,324 - -0.8 1,204,324 - -1.6 1,204,324 - -1.0 1,204,324 - +1.0 1,204,324 - -1.0 1,204,324 - +0.7 1,204,324 - -0.4 1,204,324 - -1.3 1,204,324 - -0.9 1,204,324 - -0.0
Or see this region's nucleotide sequence